Sampling device of cell culture bottle

By designing the spherical liquid storage section and a separate aspiration controller, the contamination problem of cell culture bottle sampling device during operation is solved, a cleaner and more convenient sampling process is achieved, and precise control of cell suspension volume is provided. .

CN222144605UActive Publication Date: 2024-12-10ASIA STEM CELL BK CORP LTD
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Patent Information

Application Number
CN202420373718.4
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2024-02-28
Publication Date
2024-12-10
Estimated Expiration
2034-02-28

AI Technical Summary

Technical Problem

The sampling device of the existing cell culture bottle is prone to touch the bottle mouth during operation, resulting in contamination of the bottle mouth, and the traditional design is inconvenient for cleaning and replacing the reservoir.

Method used

A sampling device including a spherical liquid storage section is designed. The diameter of the liquid storage section is larger than the bottle mouth and is held in the above part of the liquid storage section. The liquid suction controller is arranged separately from the liquid storage tube, and a vacuum suction assembly and a scale layer are used. The sealing ring has an inclined annular surface for easy connection and cleaning.

Benefits of technology

It reduces the contact between the hands and the bottle mouth, reduces the risk of contamination, keeps the liquid suction controller clean, facilitates cleaning and replacing the reservoir, and can accurately control the amount of cell suspension.

✦ Generated by Eureka AI based on patent content.

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Abstract

The utility model provides a sampling device of a cell culture bottle, which relates to the field of sampling devices, and comprises a liquid storage tube, a sampling tube, a liquid storage tube, a liquid storage tube and a liquid storage tube, and the liquid storage tube comprises a liquid suction section, a liquid storage section and a connecting section which are communicated in sequence; the liquid storage section is spherical, the diameter of the liquid storage section is larger than that of an opening of a cell culture bottle, and the liquid storage section is placed at the opening of the cell culture bottle; the liquid suction controller is detachably connected to the connecting section; a suction assembly for forming vacuum and extracting cell sap is arranged in the liquid suction controller; and the connecting assembly is arranged between the liquid suction controller and the connecting section. The sampling device has the effects that the situation that the sampling device touches the bottle opening of the culture bottle in the sampling process is reduced, and pollution to the bottle opening is reduced.
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Description

Technical Field

[0001] The present application relates to the field of sampling devices, and in particular to a sampling device for a cell culture bottle. Background Art

[0002] Cell culture flasks are a very common consumable in the biological industry. During the cell culture process, it is often necessary to monitor the status, activity, and number of cells, which requires collecting the cell suspension in the culture flask for analysis, and the collection requires a sampling device.

[0003] In the prior art, due to the small mouth of the culture bottle and the deep bottle body, when sampling with a traditional sampling device, the hand will inevitably pass over the bottle mouth, and the mouth of the culture bottle will be touched when moving the traditional sampling device, causing contamination of the bottle mouth. Utility Model Content

[0004] In order to reduce the possibility of the sampling device touching the mouth of the culture bottle during the sampling process, thereby reducing the possibility of contamination of the mouth of the bottle, the present application provides a sampling device for a cell culture bottle.

[0005] To solve the above problems, the technical solution of the utility model is:

[0006] A sampling device for a cell culture bottle, comprising:

[0007] A liquid storage tube, the liquid storage tube comprising a liquid suction section, a liquid storage section and a connecting section which are connected in sequence; the liquid storage section is spherical, and the diameter of the liquid storage section is larger than the diameter of the opening of the cell culture bottle and is used to be placed at the opening of the cell culture bottle;

[0008] A liquid suction controller is detachably connected to the connecting section; a suction component for forming a vacuum and extracting cell fluid is arranged in the liquid suction controller;

[0009] A connecting component, arranged on the liquid suction controller and the suction component, comprises a first piston body, a second piston body, a fixing part and a movable handle; two ends of the fixing part are respectively connected to the first piston body and the second piston body, the outer side walls of the first piston body and the second piston body are both in contact with the inner side wall of the liquid suction controller, and the side wall of the second piston body facing the liquid storage tube and the inner side wall of the liquid suction controller form a vacuum cavity; the vacuum cavity is connected to the inner cavity of the connecting section;

[0010] The moving handle is disposed on the first piston body and enables the first piston body to move.

[0011] In the sampling device for a cell culture bottle of the utility model, a connecting shell is arranged on the outer side wall of the liquid aspiration controller, and a gap exists between the inner wall of the connecting shell and the outer side wall of the liquid aspiration controller; a first regulating groove is arranged on the connecting shell, and the first regulating groove extends along the length direction of the liquid storage tube;

[0012] A second adjusting groove is provided on the side wall of the liquid suction controller, the second adjusting groove is connected to the gap, and the adjusting groove extends along the length direction of the first adjusting groove;

[0013] The movable handle is arranged on the connecting shell, and one end of the movable handle is located in the first adjusting groove and slides along the length direction of the first adjusting groove, and the end of the movable handle located in the first adjusting groove extends into the second adjusting groove and is connected to the first piston body.

[0014] In the sampling device for a cell culture bottle of the utility model, a scale layer is arranged on the connecting shell or the liquid aspiration controller.

[0015] In the sampling device of the cell culture bottle of the utility model, the connection assembly comprises a sealing ring, which is arranged on the inner side wall of the connection section; the sealing ring abuts against the outer side wall of the inlet end of the vacuum chamber.

[0016] In the sampling device for a cell culture bottle of the utility model, the sealing ring is provided with an inclined annular surface, and the diameter of the inclined annular surface gradually decreases from the connecting section toward the liquid aspiration section.

[0017] In the sampling device for the cell culture bottle of the utility model, the length of the liquid storage tube is A, and the value range of A is [15, 25] cm;

[0018] The diameter of the liquid suction section is L, and the value range of L is [3,7] mm.

[0019] In the sampling device of the cell culture bottle of the utility model, the material of the liquid storage tube is glass or plastic.

[0020] In the sampling device for a cell culture bottle of the utility model, the diameter of the liquid storage section is B, and the value range of B is [15, 25] mm.

[0021] Due to the adoption of the above technical solution, the utility model has the following advantages and positive effects compared with the prior art:

[0022] 1. In this solution, the liquid storage section of the liquid storage tube is set in a spherical style; when the operator moves the sampling device, he holds the liquid storage section or the part above the liquid storage section, and then extends the washing liquid section into the cell culture bottle. Due to the protruding setting of the liquid storage section, the contact between the hand and the bottle mouth of the cell culture bottle is reduced; when aspirating liquid, the liquid storage section is placed at the bottle mouth of the cell culture bottle, making it difficult for the hand to touch it, thereby reducing contamination.

[0023] On the other hand, unlike the traditional sampling device, the cell suspension is not located in the liquid pipetting controller, but is arranged in the liquid storage section, which reduces the contact between the cell suspension and the liquid pipetting controller, thereby keeping the liquid pipetting control clean;

[0024] At the same time, the liquid storage tube and the liquid suction controller are arranged separately, on the one hand, to facilitate cleaning, making cleaning more convenient; on the other hand, it is convenient to replace the liquid storage tube.

[0025] 2. The setting of the scale layer allows the operator to control the amount of cell suspension extracted.

[0026] 3. The sealing ring is provided with an inclined annular surface, which makes it more convenient to connect the liquid suction controller to the liquid storage tube. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 Schematic diagram of the overall structure of the sampling device for the cell culture bottle in the embodiment of the present application

[0028] Figure 2 Schematic diagram of the structure of the liquid aspiration controller of the sampling device for the cell culture bottle of the embodiment of the present application

[0029] Figure 3 Schematic diagram of the connection shell structure of the sampling device for the cell culture bottle in the embodiment of the present application

[0030] Figure 4 Schematic diagram of the sealing ring structure of the sampling device of the cell culture bottle in the embodiment of the present application

[0031] Explanation of the accompanying drawings: 1. Liquid storage tube; 2. Liquid suction section; 3. Liquid storage section; 4. Connecting section; 5. Liquid suction controller; 6. First piston body; 7. Second piston body; 8. Fixed part; 9. Moving handle; 10. Connecting shell; 11. First adjusting groove; 12. Second adjusting groove; 13. Scale layer; 14. Sealing ring; 15. Inclined annular surface. DETAILED DESCRIPTION

[0032] The following is a further detailed description of a sampling device for a cell culture bottle proposed by the utility model in conjunction with the accompanying drawings and specific embodiments. The advantages and features of the utility model will become more apparent according to the following description and claims.

[0033] See also Figures 1 to 4The embodiment of the present application provides a sampling device for a cell culture bottle, comprising a liquid storage tube 1, a liquid suction controller 5 and a connecting component; the liquid storage tube 1 comprises a liquid suction section 2, a liquid storage section 3 and a connecting section 4 which are connected in sequence; the liquid storage section 3 is spherical, and the diameter of the liquid storage section 3 is larger than the diameter of the opening of the cell culture bottle and is used to be placed at the opening of the cell culture bottle; the liquid suction controller 5 is detachably connected to the connecting section 4; a suction component for forming a vacuum and extracting cell fluid is arranged in the liquid suction controller 5; and the connecting component is arranged between the liquid suction controller 5 and the connecting section 4.

[0034] In this solution, the liquid storage section 3 of the liquid storage tube 1 is arranged in a spherical style; when the operator moves the sampling device, the operator holds the liquid storage section 3 or the part above the liquid storage section 3 and extends the washing liquid section into the cell culture bottle. Due to the protruding setting of the liquid storage section 3, the contact between the hand and the bottle mouth of the cell culture bottle is reduced; when aspirating liquid, the liquid storage section 3 is placed at the bottle mouth of the cell culture bottle, making it difficult for the hand to touch it, thereby reducing contamination.

[0035] On the other hand, unlike the conventional sampling device, the cell suspension is not located in the liquid pipetting controller 5, but is arranged in the liquid storage section 3, which reduces the contact between the cell suspension and the liquid pipetting controller 5, thereby keeping the liquid pipetting control clean.

[0036] At the same time, the liquid storage tube 1 and the liquid suction controller 5 are separately arranged, on the one hand, to facilitate cleaning, making cleaning more convenient; on the other hand, to facilitate the replacement of the liquid storage tube 1.

[0037] The specific structure of the sampling device for the cell culture bottle of this embodiment is further described below:

[0038] In this embodiment, the suction assembly includes a first piston body 6, a second piston body 7, a fixed portion 8 and a movable handle 9; the two ends of the fixed portion 8 are respectively connected to the first piston body 6 and the second piston body 7, the outer side walls of the first piston body 6 and the second piston body 7 are both in contact with the inner wall of the liquid suction controller 5, and the second piston body 7 forms a vacuum cavity toward the side wall of the liquid storage tube 1 and the inner wall of the liquid suction controller 5; the vacuum cavity is connected to the inner cavity of the connecting end;

[0039] The moving handle 9 is disposed on the first piston body 6 and enables the first piston body 6 to move.

[0040] To further explain, the first piston body 6, the fixing portion 8 and the second piston body 7 are combined to form an I-shaped cross-section, and the connecting chamber formed by the inner wall of the liquid suction controller 5 and the side wall of the first piston body 6 facing away from the liquid storage tube 1 is connected to the outside atmosphere, so that the first piston body 6 can move in the liquid suction controller 5.

[0041] To further explain, the movable handle 9 may be disposed on the first piston body 6 and located in the communicating chamber.

[0042] In this embodiment, a connecting shell 10 is provided on the outer side wall of the liquid suction controller 5, and there is a gap between the inner side wall of the connecting shell 10 and the outer side wall of the liquid suction controller 5; a first adjusting groove 11 is provided on the connecting shell 10, and the first adjusting groove 11 extends along the length direction of the liquid storage tube 1;

[0043] A second adjusting groove 12 is provided on the side wall of the liquid suction controller 5. The second adjusting groove 12 is connected to the gap, and the adjusting groove extends along the length direction of the first adjusting groove 11.

[0044] The movable handle 9 is arranged on the connecting shell 10 , and one end of the movable handle 9 is located in the first adjusting groove 11 and slides along the length direction of the first adjusting groove 11 , and the end of the movable handle 9 located in the first adjusting groove 11 extends into the second adjusting groove 12 and is connected to the first piston body 6 .

[0045] It is further configured that, since the second regulating groove 12 is opened on the side wall of the liquid suction controller 5, there is a risk of the vacuum chamber and the second regulating groove 12 being connected; therefore, during the design process, when the liquid storage tube 1 is vertically arranged and the liquid suction controller 5 is located above the liquid storage tube 1, the highest point of the second piston body 7 movement is lower than the lowest point of the second regulating groove 12.

[0046] In this embodiment, a scale layer 13 is provided on the connecting shell 10 or the liquid pipetting controller 5 .

[0047] It is further explained that the setting of the scale layer 13 allows the operator to accurately control the amount of the aspirated cell suspension.

[0048] In this embodiment, the connection assembly includes a sealing ring 14, which is disposed on the inner wall of the connection section 4; the sealing ring 14 abuts against the outer wall of the inlet end of the vacuum chamber.

[0049] In this embodiment, the sealing ring 14 is provided with an inclined annular surface 15 , and the diameter of the inclined annular surface 15 gradually decreases from the connecting section 4 toward the liquid suction section 2 .

[0050] Furthermore, the sealing ring 14 may also be arranged at the liquid inlet opening of the liquid suction controller 5 . In this case, the diameter of the inclined annular surface 15 gradually increases from the connecting section 4 toward the liquid suction section 2 .

[0051] To further explain, when the connecting section 4 and the liquid suction controller 5 are connected, the inclined annular surface 15 is first used as a guide surface to facilitate insertion, and then the sealing ring 14 is used to achieve sealing.

[0052] In this embodiment, the length of the liquid storage tube 1 is A, and the value range of A is [15, 25] cm;

[0053] The diameter of the liquid suction section 2 is L, and the value range of L is [3,7] mm.

[0054] In this embodiment, the liquid storage tube 1 is made of glass or plastic.

[0055] It is further configured that when the liquid storage tube 1 is made of glass, it can be reused by cleaning and high-temperature sterilization; when the liquid storage tube 1 is made of plastic, the liquid storage tube 1 can be easily separated and discarded through a connecting component.

[0056] In this embodiment, the diameter of the liquid storage section 3 is B, and the value range of B is [15, 25] mm.

[0057] The above is a detailed description of the embodiments of the present invention in conjunction with the accompanying drawings, but the present invention is not limited to the above embodiments. Even if various changes are made to the present invention, if these changes fall within the scope of the claims of the present invention and their equivalents, they still fall within the protection scope of the present invention.

Claims

1. A sampling device for a cell culture bottle, characterized in that: include, A liquid storage tube, the liquid storage tube comprising a liquid suction section, a liquid storage section and a connecting section which are connected in sequence; the liquid storage section is spherical, and the diameter of the liquid storage section is larger than the diameter of the opening of the cell culture bottle and is used to be placed at the opening of the cell culture bottle; A liquid suction controller is detachably connected to the connecting section; a suction component for forming a vacuum and extracting cell fluid is arranged in the liquid suction controller; A connecting component is arranged between the liquid suction controller and the connecting section.

2. The sampling device for a cell culture bottle according to claim 1, characterized in that: The suction assembly comprises a first piston body, a second piston body, a fixing part and a movable handle; two ends of the fixing part are respectively connected to the first piston body and the second piston body, the outer side walls of the first piston body and the second piston body are both in contact with the inner side wall of the liquid suction controller, and the side wall of the second piston body facing the liquid storage tube and the inner side wall of the liquid suction controller form a vacuum cavity; the vacuum cavity is connected to the inner cavity of the connecting section; The moving handle is disposed on the first piston body and enables the first piston body to move.

3. The sampling device for a cell culture bottle according to claim 2, characterized in that: A connecting shell is provided on the outer side wall of the liquid pipetting controller, and a gap exists between the inner wall of the connecting shell and the outer side wall of the liquid pipetting controller; a first adjusting groove is provided on the connecting shell, and the first adjusting groove extends along the length direction of the liquid storage tube; A second adjusting groove is provided on the side wall of the liquid suction controller, the second adjusting groove is connected to the gap, and the adjusting groove extends along the length direction of the first adjusting groove; The movable handle is arranged on the connecting shell, and one end of the movable handle is located in the first adjusting groove and slides along the length direction of the first adjusting groove, and the end of the movable handle located in the first adjusting groove extends into the second adjusting groove and is connected to the first piston body.

4. The sampling device for a cell culture bottle according to claim 3, characterized in that: A scale layer is provided on the connecting shell or the liquid pipetting controller.

5. The sampling device for a cell culture bottle according to claim 2, characterized in that: The connection assembly comprises a sealing ring, which is arranged on the inner side wall of the connection section; the sealing ring abuts against the outer side wall of the inlet end of the vacuum chamber.

6. The sampling device for a cell culture bottle according to claim 5, characterized in that: The sealing ring is provided with an inclined annular surface, and the diameter of the inclined annular surface gradually decreases from the connecting section to the liquid suction section.

7. The sampling device for a cell culture bottle according to claim 1, characterized in that: The length of the liquid storage tube is A, and the value range of A is [15, 25] cm; The diameter of the liquid suction section is L, and the value range of L is [3,7] mm.

8. The sampling device for a cell culture bottle according to claim 1, characterized in that: The material of the liquid storage tube is glass or plastic.

9. The sampling device for a cell culture bottle according to claim 1, characterized in that: The diameter of the liquid storage section is B, and the value range of B is [15,25] mm.