Phospholipase activity detection device

Abstract

The utility model provides a phospholipase activity detection device which comprises a cleaning assembly, the cleaning assembly comprises a cuvette, two clamping seats, a servo motor, a lifting frame, a mounting seat, an electric telescopic rod, a mounting frame, an electric turntable, a high-pressure pump, a spray hole, a spray head, a sewage collection box and a clean water tank; the two clamping seats are fixedly connected to the upper part of the outer side wall of the cuvette through screws. According to the cuvette cleaning device, under the cooperation of the electric telescopic rod, the electric rotating disc and the servo motor, the cuvette can be inverted, the cuvette is connected to the outer portion of the spray head in a sleeving mode, cleaning liquid is conveyed through the high-pressure pump, the cuvette can be cleaned, after cleaning is completed, the cuvette is reset under the cooperation of the electric telescopic rod, the servo motor and the electric rotating disc, and then the cuvette is cleaned. Compared with the prior art, the cuvette detection device has the advantages that the labor intensity of detection personnel is reduced, and the situation that the cuvette is collided and scratched is avoided.

Description

Technical Field

[0001] The utility model relates to a detection device, in particular to a phospholipase activity detection device, belonging to the technical field of detection devices. Background Art

[0002] Phospholipases are a class of enzymes that exist in organisms and have the function of hydrolyzing glycerophospholipids. They mainly include phospholipases A1, A2, C and D, which are widely present in animals and plants. As a potential target for drug action, the detection of phospholipases' activity is of great significance for the development of new drugs. By screening compounds that can regulate the activity of phospholipases, new drugs with therapeutic potential can be developed, providing new means for the treatment of diseases.

[0003] Enzyme-linked colorimetry is one of the methods for detecting phospholipase activity. It is a method for determining phospholipase activity based on the generation of choline by phospholipase-catalyzed hydrolysis of the phosphodiester bond at the end of phosphatidylcholine, and the generation of H2O2 as a benchmark. This method is based on a specific chemical reaction and determines the activity of phospholipase by colorimetry. During the test, the generated colored product is transferred to a cuvette, and then the sample is tested using a spectrophotometer to calculate the absorbance of the sample, and then the activity of the phospholipase is inferred based on the absorbance.

[0004] At present, when using a spectrophotometer to detect the activity of phospholipase, multiple groups of tests are often required. Through multiple groups of tests, the absorbance data of these samples can be obtained, and then their phospholipase activity differences can be compared, which is of great significance for revealing the function, mechanism of action and influencing factors of phospholipase under different conditions. The spectrophotometer in the prior art requires manual cleaning of the colorimetric dish. When conducting multiple groups of tests, multiple cleanings will not only increase the labor intensity of the test personnel, but also easily cause the colorimetric dish to be bumped and scratched during manual cleaning, thereby affecting the test results. Therefore, a phospholipase activity detection device is proposed. Utility Model Content

[0005] In view of this, the utility model provides a phospholipase activity detection device to solve or alleviate the technical problems existing in the prior art and at least provide a beneficial choice.

[0006] The technical solution of the embodiment of the utility model is implemented as follows: a phospholipase activity detection device, including a cleaning component, the cleaning component includes a cuvette, two holders, a servo motor, a lifting frame, a mounting seat, an electric telescopic rod, a mounting frame, an electric turntable, a high-pressure pump, a spray hole, a spray head, a sewage collection box and a clean water tank;

[0007] The two holders are fixedly connected to the upper part of the outer wall of the cuvette by screws, one holder is installed on the output shaft of the servo motor, the servo motor is installed on one side of the lifting frame, the mounting seat is installed on the front surface of the mounting frame, the electric telescopic rod is installed on the lower surface of the mounting seat, and the upper surface of the mounting seat is symmetrically fixedly connected with two guide rods, the bottom end of the mounting frame is installed at the center of the upper surface of the electric turntable, the bottom end of the nozzle is installed at the center of the inner bottom wall of the sewage collection box, the spray holes are equidistantly arranged on the outer wall of the nozzle, and the high-pressure pump is installed on one side of the clean water tank.

[0008] Further preferably, the sewage collecting box is installed on the upper surface of the clean water tank, and a sewage pipe is installed on the outer side wall of the sewage collecting box.

[0009] Further preferably, the water inlet of the high-pressure pump is connected to the water outlet of the clean water tank through a pipe, and the water outlet of the high-pressure pump is connected to the water inlet of the nozzle through a pipe.

[0010] Further preferably, the top end of the guide rod is fixedly connected to the inner top wall of the mounting frame, and the lifting frame is slidably connected to the outer side walls of the two guide rods.

[0011] Further preferably, the telescopic shaft of the electric telescopic rod is fixedly connected to the lower surface of the lifting frame.

[0012] Further preferably, a main body component is installed on the lower surface of the electric turntable, and the main body component includes a spectrophotometer, an operation panel, a cover plate, a base and a detection port;

[0013] The spectrophotometer is installed on the upper surface of the base, the operation panel is installed on the front surface of the spectrophotometer, the detection port is opened on the upper surface of the spectrophotometer, and the cover plate is installed on the upper surface of the spectrophotometer.

[0014] Further preferably, the electric turntable and the cleaning water tank are both installed on the upper surface of the base, and a controller is installed on the upper surface of the base.

[0015] Further preferably, the cuvette is located inside the detection port, the position of the cover plate corresponds to the position of the detection port, and the position of the nozzle corresponds to the position of the cuvette.

[0016] Since the embodiment of the utility model adopts the above technical scheme, it has the following advantages: the utility model detects the absorbance of the sample by a spectrophotometer, and then calculates the activity of phospholipase according to the absorbance analysis. When a group of sample detection is completed, the cover plate is opened, and the cuvette can be inverted with the cooperation of the electric telescopic rod, the electric turntable and the servo motor, and the cuvette is sleeved on the outside of the nozzle. The cleaning liquid is transported by a high-pressure pump to clean the cuvette, and the sewage is collected by the sewage collection box to avoid pollution of the surrounding environment. When the cleaning is completed, the cuvette is reset with the cooperation of the electric telescopic rod, the servo motor and the electric turntable, and the detection personnel can continue to add samples into the cuvette for detection. Compared with the prior art, the labor intensity of the detection personnel is reduced, and the cuvette will not be bumped or scratched.

[0017] The above summary is for the purpose of description only and is not intended to be limiting in any way. In addition to the illustrative aspects, embodiments and features described above, further aspects, embodiments and features of the utility model will be readily apparent by reference to the accompanying drawings and the following detailed description. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0019] Figure 1 It is a structural diagram of the utility model;

[0020] Figure 2 This is a structural diagram of the main components of the utility model;

[0021] Figure 3 This is a schematic diagram of the connection between the card holder and the cuvette of the utility model;

[0022] Figure 4 This is a structural diagram of the nozzle of the utility model;

[0023] Figure 5 It is a schematic diagram of the cuvette of the utility model when being cleaned.

[0024] Figure numerals: 101, cleaning component; 11, cuvette; 12, holder; 13, servo motor; 14, lifting frame; 15, guide rod; 16, mounting seat; 17, electric telescopic rod; 18, mounting frame; 19, electric turntable; 20, high-pressure pump; 21, sewage pipe; 22, spray hole; 23, nozzle; 24, sewage collection box; 25, clean water tank; 301, main body component; 31, spectrophotometer; 32, operation panel; 33, cover plate; 34, base; 35, controller; 36, detection port. DETAILED DESCRIPTION

[0025] In the following, only some exemplary embodiments are briefly described. As those skilled in the art will appreciate, the described embodiments may be modified in various ways without departing from the spirit or scope of the present invention. Therefore, the drawings and descriptions are considered to be exemplary and non-restrictive in nature.

[0026] The embodiments of the present utility model are described in detail below with reference to the accompanying drawings.

[0027] like Figure 1-5 As shown, the embodiment of the utility model provides a phospholipase activity detection device, including a cleaning component 101, the cleaning component 101 includes a cuvette 11, two holders 12, a servo motor 13, a lifting frame 14, a mounting seat 16, an electric telescopic rod 17, a mounting frame 18, an electric turntable 19, a high-pressure pump 20, a spray hole 22, a spray head 23, a sewage collection box 24 and a clean water tank 25;

[0028] Two holders 12 are fixedly connected to the upper part of the outer wall of the cuvette 11 by screws, one holder 12 is installed on the output shaft of the servo motor 13, the servo motor 13 is installed on one side of the lifting frame 14, the mounting seat 16 is installed on the front surface of the mounting frame 18, the electric telescopic rod 17 is installed on the lower surface of the mounting seat 16, and the upper surface of the mounting seat 16 is symmetrically fixedly connected with two guide rods 15, the bottom end of the mounting frame 18 is installed at the center of the upper surface of the electric turntable 19, the bottom end of the nozzle 23 is installed at the center of the inner bottom wall of the sewage collection box 24, the spray holes 22 are equidistantly arranged on the outer wall of the nozzle 23, and the high-pressure pump 20 is installed on one side of the clean water tank 25.

[0029] In one embodiment, a sewage collection box 24 is installed on the upper surface of a clean water tank 25, and a sewage pipe 21 is installed on the outer wall of the sewage collection box 24. The sewage collection box 24 can be used to collect samples in the cuvette 11, and the sewage generated during cleaning can also be collected and then discharged through the sewage pipe 21.

[0030] In one embodiment, the water inlet of the high-pressure pump 20 is connected to the water outlet of the cleaning water tank 25 through a pipe, and the water outlet of the high-pressure pump 20 is connected to the water inlet of the nozzle 23 through a pipe, so that when the high-pressure pump 20 is working, the nozzle 23 sprays cleaning liquid through the spray hole 22.

[0031] In one embodiment, the top end of the guide rod 15 is fixedly connected to the inner top wall of the mounting frame 18, the lifting frame 14 is slidably connected to the outer side walls of the two guide rods 15, and the telescopic shaft of the electric telescopic rod 17 is fixedly connected to the lower surface of the lifting frame 14. The lifting frame 14 is driven by the electric telescopic rod 17 to control the height position of the cuvette 11, and the lifting frame 14 can be guided by the guide rod 15 so that the lifting frame 14 can be lifted and lowered vertically.

[0032] In one embodiment, a main body assembly 301 is installed on the lower surface of the electric turntable 19, and the main body assembly 301 includes a spectrophotometer 31, an operation panel 32, a cover 33, a base 34 and a detection port 36;

[0033] The spectrophotometer 31 is installed on the upper surface of the base 34, the operation panel 32 is installed on the front surface of the spectrophotometer 31, the detection port 36 is opened on the upper surface of the spectrophotometer 31, and the cover 33 is installed on the upper surface of the spectrophotometer 31. The spectrophotometer 31 detects the sample in the colorimetric dish 11 to calculate the absorbance of the sample, and then the activity of the phospholipase is calculated according to the absorbance.

[0034] In one embodiment, the electric turntable 19 and the cleaning water tank 25 are both installed on the upper surface of the base 34, and a controller 35 is installed on the upper surface of the base 34. The electrical output end of the controller 35 is electrically connected to the electrical input ends of the servo motor 13, the electric telescopic rod 17, the electric turntable 19 and the high-pressure pump 20 through a relay. The electrical input end of the controller 35 is connected to an external power supply to power the servo motor 13, the electric telescopic rod 17, the electric turntable 19 and the high-pressure pump 20. The model of the controller 35 is: OHR-PR10.

[0035] In one embodiment, the cuvette 11 is located inside the detection port 36, the position of the cover 33 corresponds to the position of the detection port 36, and the position of the nozzle 23 corresponds to the position of the cuvette 11, so that the detection port 36 can be sealed by the cover 33 for absorbance detection.

[0036] When the utility model is working, the cuvette 11 is installed inside the holder 12, and the colored product generated by the reaction is dripped into the cuvette 11, then the electric telescopic rod 17 is shortened, the electric telescopic rod 17 drives the lifting frame 14, and the lifting frame 14 drives the cuvette 11 to move into the detection port 36, and the detection work can be carried out after the cover plate 33 is covered, the absorbance of the sample is detected by the spectrophotometer 31, and then the activity of the phospholipase is calculated according to the absorbance analysis. When a group of sample tests are completed, the cover plate 33 is opened, and the electric telescopic rod 17 is extended, and the lifting frame 14 drives the cuvette 11 to move out of the detection port 36, and the electric turntable 19 rotates ninety degrees clockwise, and then the servo motor 13 drives the holder 12 to rotate one hundred and eighty degrees clockwise. At this time, the cuvette 11 is moved out of the detection port 36. The cuvette 11 is inverted, and the sample inside is poured into the sewage collection box 24. The cuvette 11 is located directly above the nozzle 23, and then the electric telescopic rod 17 is reset. The cuvette 11 is sleeved on the outside of the nozzle 23, and then the high-pressure pump 20 works, and the cleaning liquid in the clean water tank 25 is sprayed out through the spray hole 22. The water flow of the sprayed water can clean the cuvette 11. The sewage generated during cleaning flows into the sewage collection box 24, and then the sewage is collected through the sewage pipe 21. When the cleaning is completed, the electric telescopic rod 17 moves upward, the cuvette 11 is away from the nozzle 23, and then the servo motor 13 rotates one hundred and eighty degrees counterclockwise, and then the electric turntable 19 rotates ninety degrees counterclockwise. At this time, the cleaned cuvette 11 is reset, and samples can continue to be added for detection.

[0037] The above is only a specific implementation of the utility model, but the protection scope of the utility model is not limited thereto. Any technician familiar with the technical field can easily think of various changes or substitutions within the technical scope disclosed by the utility model, which should be included in the protection scope of the utility model. Therefore, the protection scope of the utility model should be based on the protection scope of the claims.

Claims

1. A phospholipase activity detection device, comprising a cleaning component (101), characterized in that: The cleaning assembly (101) comprises a cuvette (11), two holders (12), a servo motor (13), a lifting frame (14), a mounting seat (16), an electric telescopic rod (17), a mounting frame (18), an electric turntable (19), a high-pressure pump (20), a spray hole (22), a spray head (23), a sewage collection box (24) and a clean water tank (25); The two holders (12) are fixedly connected to the upper part of the outer wall of the cuvette (11) by screws, one holder (12) is mounted on the output shaft of the servo motor (13), the servo motor (13) is mounted on one side of the lifting frame (14), the mounting seat (16) is mounted on the front surface of the mounting frame (18), the electric telescopic rod (17) is mounted on the lower surface of the mounting seat (16), the upper surface of the mounting seat (16) is symmetrically fixedly connected with two guide rods (15), the bottom end of the mounting frame (18) is mounted on the center of the upper surface of the electric turntable (19), the bottom end of the nozzle (23) is mounted on the center of the inner bottom wall of the sewage collection box (24), the spray holes (22) are equidistantly arranged on the outer wall of the nozzle (23), and the high-pressure pump (20) is mounted on one side of the clean water tank (25).

2. A phospholipase activity detection device according to claim 1, characterized in that: The sewage collection box (24) is installed on the upper surface of the clean water tank (25), and a sewage discharge pipe (21) is installed on the outer side wall of the sewage collection box (24).

3. A phospholipase activity detection device according to claim 2, characterized in that: The water inlet of the high-pressure pump (20) is connected to the water outlet of the clean water tank (25) through a pipeline, and the water outlet of the high-pressure pump (20) is connected to the water inlet of the nozzle (23) through a pipeline.

4. The phospholipase activity detection device according to claim 1, characterized in that: The top end of the guide rod (15) is fixedly connected to the inner top wall of the mounting frame (18), and the lifting frame (14) is slidably connected to the outer side walls of the two guide rods (15).

5. A phospholipase activity detection device according to claim 4, characterized in that: The telescopic shaft of the electric telescopic rod (17) is fixedly connected to the lower surface of the lifting frame (14).

6. A phospholipase activity detection device according to claim 1, characterized in that: A main body component (301) is installed on the lower surface of the electric turntable (19), and the main body component (301) includes a spectrophotometer (31), an operation panel (32), a cover plate (33), a base (34) and a detection port (36); The spectrophotometer (31) is mounted on the upper surface of the base (34), the operation panel (32) is mounted on the front surface of the spectrophotometer (31), the detection port (36) is opened on the upper surface of the spectrophotometer (31), and the cover plate (33) is mounted on the upper surface of the spectrophotometer (31).

7. A phospholipase activity detection device according to claim 6, characterized in that: The electric turntable (19) and the cleaning water tank (25) are both mounted on the upper surface of a base (34), and a controller (35) is mounted on the upper surface of the base (34).

8. The phospholipase activity detection device according to claim 6, characterized in that: The cuvette (11) is located inside the detection port (36), the position of the cover plate (33) corresponds to the position of the detection port (36), and the position of the nozzle (23) corresponds to the position of the cuvette (11).

Images