Pressure-adjustable wet-process automatic column pressing device

Abstract

The utility model relates to the technical field of experimental instruments and devices, and discloses a pressure-adjustable wet-process automatic column pressing device which comprises a column pipe and two spherical buffers, the bottom of the first buffer is communicated with the upper end of the column pipe, the lower end of the column pipe is connected with a straight tee joint, and the lower end of the straight tee joint is connected with a liquid outlet through a first guide pipe. The side end of the straight tee joint is communicated with a side inlet of a second buffer through a second guide pipe, a bottom outlet of the second buffer is communicated with an eluent circulation inlet through a third guide pipe, a circulation loop is provided for eluent circulation, a drain valve is arranged on the first guide pipe, and a first eluent circulation valve is arranged on the second guide pipe. The air inlet is connected with a compression pump through a fourth guide pipe, through pressurization and control of a control valve on the circulation loop, recycling of the eluent is achieved, the trouble that the eluent is manually and repeatedly added for multiple times is avoided, and the device has the advantages of being simple in structure, high in practicability and good in using effect.

Description

Technical Field

[0001] The utility model relates to the technical field of experimental instrument devices, in particular to a wet-method automatic column pressing device with adjustable pressure. Background Art

[0002] Column chromatography is a common method for laboratory separation and purification, and is also one of the most efficient methods for laboratory separation. Neutral alumina powder and silica gel powder are often used for column compression. At present, the common methods of column compression in laboratories are mainly dry column loading and wet column loading. Dry column loading mainly uses a vacuum pump to create negative pressure on the bottom of the column, compact the bottom of the column, pour in the solvent, and manually compact the column. The disadvantage is that solvent heat will be generated when the solvent is added, and low-boiling eluents will cause local bubbles. Wet column loading will not cause local bubbles caused by solvent heat effect. It mainly uses manual column compression and electromagnetic compression pump column compression. The disadvantage is that manual column compression consumes more manpower and the degree of column compression is slightly different each time. In general, manual column compression is a waste of time and will cause human errors. The emergence of electromagnetic compression pump column compression not only saves manpower, liberates the hands of the experimenter, but also avoids human errors in the degree of column compression. However, the only drawback is that the eluent needs to be added manually to achieve circulation until the column is compacted.

[0003] In order to solve the above problems, the utility model provides a wet automatic column pressing device with adjustable pressure, which realizes the recycling of eluent and avoids the disadvantage of repeated manual addition of eluent. Utility Model Content

[0004] The utility model aims to provide a wet automatic column pressing device with adjustable pressure to realize the recycling of eluent, and has the characteristics of simple structure, easy operation, low cost and strong practicality.

[0005] In order to achieve the above purpose, the utility model provides the following technical solutions:

[0006] A wet automatic column pressing device with adjustable pressure comprises a column tube and two spherical buffers, wherein a feed port, an air inlet, a gas regulating port and an eluent circulation inlet are arranged on the first buffer, the gas regulating port is located at the top of the first buffer, and a gas regulating valve is arranged on the gas regulating port, the bottom of the first buffer is connected with the upper end of the column tube, the lower end of the column tube is connected with a straight tee, the lower end of the straight tee is connected with a liquid discharge port through a first conduit, a side inlet and a bottom outlet are arranged on the second buffer, the side end of the straight tee is connected with the side inlet of the second buffer through a second conduit, the bottom outlet of the second buffer is connected with the eluent circulation inlet through a third conduit, a liquid discharge valve is arranged on the first conduit, a first eluent circulation valve is arranged on the second conduit, a second eluent circulation valve is arranged on the third conduit, and the air inlet is connected with a compression pump through a fourth conduit.

[0007] The compression pump is a positive displacement electromagnetic compression pump or a positive displacement diaphragm compression pump.

[0008] The feed port, the air inlet, the gas regulating port and the eluent circulation inlet are all interfaces made of high borosilicate glass or stainless steel.

[0009] The gas regulating valve, eluent circulation valve and drain valve are all pneumatic joint valves.

[0010] The beneficial effects achieved by the utility model compared with the prior art are:

[0011] The utility model arranges a feed port, an air inlet, a gas regulating port and an eluent circulation inlet on the first buffer, and provides a circulation loop for the eluent circulation through a three-way, an eluent circulation valve and a second buffer, so as to realize the recycling of the eluent and avoid the trouble of manually adding the eluent repeatedly. The utility model has the characteristics of simple structure, strong practicality and good use effect, and is worthy of wide promotion and use. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] Figure 1 It is a schematic diagram of the structure of the utility model;

[0013] Figure 2 for Figure 1 Schematic diagram of the interface connector structure. DETAILED DESCRIPTION

[0014] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0015] Example

[0016] See also Figure 1A wet automatic column pressing device with adjustable pressure comprises a column tube 1 and two spherical buffers 2 and 3. A feed port 21, an air inlet 23, a gas regulating port 22 and an eluent circulation inlet 24 are arranged on the first buffer 2. The eluent and the chromatography powder are added through the feed port 21. A glass stopper 4 is arranged on the feed port 21 to seal the feed port 21. The gas regulating port 22 is located at the top of the first buffer 2, and a gas regulating valve 5 is arranged on the gas regulating port 22. The gas regulating valve 5 is connected to the gas regulating port 22 through a pipeline and an interface connector 6. The bottom of the first buffer 2 is connected to the upper end of the column tube 1 through a connecting port 25. The lower end of the column tube 1 is connected to a straight tee 7. The lower end of the straight tee 7 is connected to a drain port through a first conduit. The second buffer 3 is provided with a side inlet 31 and a bottom outlet The side end of the straight tee 7 is connected with the side inlet 31 of the second buffer 3 through the second conduit, the bottom outlet 32 ​​of the second buffer 3 is connected with the eluent circulation inlet 24 of the first buffer 2 through the third conduit, a drain valve 8 is arranged on the first conduit, an eluent circulation valve one 9 is arranged on the second conduit, and an eluent circulation valve two 10 is arranged on the third conduit. The air inlet 23 is connected with a compression pump 11 through a fourth conduit. The compression pump preferably adopts a volumetric electromagnetic compression pump or a volumetric diaphragm compression pump. The feed port 21, the air inlet 23, the gas regulating port 22, the eluent circulation inlet 24, the side inlet 31 and the bottom outlet 32 ​​are preferably made of high borosilicate glass or stainless steel interfaces, and the gas regulating valve 5, the eluent circulation valve and the drain valve 8 are preferably pneumatic joint valves.

[0017] See also Figure 2 The interface connector 6 is provided with multiple sealing rings 61, and the air inlet 23, the gas regulating port 22, the eluent circulation inlet 24, the side inlet 31 and the bottom outlet 32 ​​are all connected to the corresponding conduits using the same interface connector.

[0018] Principle and use process of this utility model:

[0019] Add eluent and chromatography powder into the first buffer 2 and column tube 1 through the feed port until the liquid level reaches about 1 cm from the bottom of the air inlet of the first buffer 2, plug in a glass stopper, and clamp it with a clamp. Close the drain valve 8, open the gas regulating valve 5 and the eluent circulation valve 9, install the interface connector of the inlet gas 23 and clamp it with a clamp, and turn on the switch of the compression pump 11. Slowly close the opening of the gas regulating valve 5 to control the pressure. Preferably, a pressure gauge can be set between the gas regulating valve 5 and the corresponding interface connector 6 to visually observe the pressure change. During the column pressing process, the air in the second buffer 3 is compressed, and the eluent is pressed into the second buffer 3 for temporary storage. During this process, the height of the chromatographic powder in the column tube 1 is observed and replenished in time. When the pressure reaches a certain level or the eluent in the second buffer 3 reaches a certain amount, the gas regulating valve 5 and the eluent circulation valve 10 are opened to make the eluent temporarily stored in the second buffer 3 flow back to the first buffer 2 and the column tube 1, and the eluent is circulated in sequence to realize the recycling of the eluent. It only needs to control the valve, which avoids the trouble of repeatedly opening the device to circulate and add eluent in the prior art.

[0020] Keep the compression pump in working condition, and after the chromatographic powder height no longer changes and the column is compacted, slowly open the gas regulating valve and the drain valve to allow the eluent to flow out slowly, and retain a certain amount of eluent in the column.

[0021] The above are preferred embodiments of the present application, and the protection scope of the present application is not limited thereto. Therefore, any equivalent changes made according to the structure, shape, and principle of the present application should be included in the protection scope of the present application.

Claims

1. A wet automatic column pressing device with adjustable pressure, characterized in that: The utility model comprises a column tube and two spherical buffers, wherein a feed port, an air inlet, a gas regulating port and an eluent circulation inlet are arranged on the first buffer, the gas regulating port is located at the top of the first buffer, and a gas regulating valve is arranged on the gas regulating port, the bottom of the first buffer is connected with the upper end of the column tube, the lower end of the column tube is connected with a straight tee, the lower end of the straight tee is connected with a liquid discharge port through a first conduit, a side inlet and a bottom outlet are arranged on the second buffer, the side end of the straight tee is connected with the side inlet of the second buffer through a second conduit, the bottom outlet of the second buffer is connected with the eluent circulation inlet through a third conduit, a liquid discharge valve is arranged on the first conduit, a first eluent circulation valve is arranged on the second conduit, a second eluent circulation valve is arranged on the third conduit, and the air inlet is connected with a compression pump through a fourth conduit.

2. The column pressing device according to claim 1, characterized in that: The compression pump is a positive displacement electromagnetic compression pump or a positive displacement diaphragm compression pump.

3. The column pressing device according to claim 1, characterized in that: The feed port, the air inlet, the gas regulating port and the eluent circulation inlet are all interfaces made of high borosilicate glass or stainless steel.

4. The column pressing device according to claim 1, characterized in that: The gas regulating valve, eluent circulation valve and drain valve are all pneumatic joint valves.

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