Cell culture dish and storage incubator thereof
By setting up an air filter membrane and a clamping structure on the cell culture dish, the problem of air pollution during the handling process of cell culture dishes is solved, and the stable storage and prevention of cell culture dishes are achieved through the design of trays and limiting blanks. Contamination improves cell quality and storage safety.
Patent Information
- Application Number
- CN202420323242.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-02-21
- Publication Date
- 2024-12-17
- Estimated Expiration
- 2034-02-21
AI Technical Summary
During cell culture, cell culture dishes are susceptible to external air pollution during the handling process, resulting in cell contamination problems.
A cell culture dish including the body of the Petri dish, cover, air filter membrane and jamming components and its storage incubator are designed. By setting the air filter membrane and jamming structure on the petri dish cover, it ensures air filtration during the handling process. The membrane filters the outside air to prevent cells from contacting with the outside air and uses trays and limiting pads for stable support and storage in the incubator.
It effectively prevents external air pollution, improves the guarantee of cell quality, and ensures stable storage of the Petri dish through the design of trays and limiting baffles, and avoids the problem of tilting and splashing.
Smart Images
Figure CN222182252U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of cell culture, in particular to a cell culture dish and a storage culture box thereof. Background Art
[0002] Cell culture dishes are the preferred consumables for cell expansion and culture, especially for some cells that are difficult to digest or difficult to disperse after digestion. They are particularly convenient to handle during the cell culture process.
[0003] During the cell culture process, the cell culture dishes after cell inoculation are directly placed in the incubator for culture. The cell culture dishes processed in the clean bench are taken to the incubator through the external environment for storage.
[0004] The utility model with the publication number CN209796100U proposes a pull-out double-layer cell culture dish sample storage box, including a box body, wherein two placement cavities are arranged above and below the box body, a mounting seat is slidably installed in each placement cavity, and a plurality of cell culture dishes are arranged on each mounting seat, and a fixing device is arranged between the top of each mounting seat and the placement cavity, and each fixing device includes a first sliding cavity arranged on the top wall of the placement cavity, a fixing plate is slidably installed in each first sliding cavity, and a second sliding cavity is respectively arranged on both sides of each first sliding cavity. When the cell culture dishes of the above-mentioned sample storage box are not provided with an air passing structure, specifically when the cell culture dishes are taken to the incubator through the external environment, the external air can enter the interior of the culture dishes, thereby causing cell contamination, so a cell culture dish and a storage incubator thereof are proposed to solve the above-mentioned problem. Utility Model Content
[0005] In view of this, the utility model proposes a cell culture dish and a storage culture box thereof. Specifically, when the culture dish body is transported, the culture dish cover is closed on the top of the culture dish body in advance. At this time, the culture dish cover is snap-fitted with the culture dish body through a snap-fit structure, thereby completing the rapid positioning of the culture dish cover. At this time, the air filter membrane can filter out external air to prevent the cells stored in the culture dish body from contacting with external air during transportation, thereby preventing external air from contaminating the culture dish body.
[0006] The technical solution of the utility model is implemented as follows: The utility model provides a cell culture dish and a storage culture box thereof, including a culture dish body, a culture dish cover, an air filter membrane and a clamping component, wherein:
[0007] A culture dish cover, which is detachably connected to the culture dish body and covers the culture dish body, and the culture dish cover is provided with a first through hole connected to the culture dish body;
[0008] an air filter membrane, arranged at the inner side of the first through hole and covering the first through hole;
[0009] The clamping component is arranged on the culture dish body and is used for clamping the culture dish cover.
[0010] On the basis of the above technical solution, preferably, the culture dish body includes an annular protrusion, which is located on the top of the culture dish body and inserted into the inner side of the culture dish cover to fit with the inner wall of the culture dish cover, and the clamping component is arranged on the annular protrusion.
[0011] On the basis of the above technical solution, preferably, the clamping component includes a clamping ball and a spring, wherein:
[0012] The annular protrusion is provided with a plurality of mounting grooves, the clamping ball is slidably arranged inside the plurality of mounting grooves, and the inner wall of the culture dish cover is provided with a clamping groove for the clamping ball to be inserted;
[0013] A spring is arranged between the locking ball and the mounting groove and is used for resetting the sliding position of the locking ball.
[0014] On the basis of the above technical solution, preferably, it also includes a gusset plate, wherein:
[0015] A plurality of buckle grooves are provided on the peripheral side of the culture dish body, and the buckle plates are arranged inside the buckle grooves and form gaps between the buckle plates and the buckle grooves.
[0016] The present invention also provides a storage incubator for cell culture dishes, comprising the above-mentioned cell culture dishes, a culture box body, a transfer door and a sealing insert ring, wherein:
[0017] The culture box is used to store the culture dish body, and the transfer door is rotatably arranged on the culture box to cover the culture box;
[0018] The sealing insert ring is a rubber insert ring. When the transfer door covers the culture box body, the sealing insert ring is inserted into the inner side of the culture dish body and fits with the inner wall of the culture dish body.
[0019] On the basis of the above technical solution, preferably, it comprises a plurality of trays, wherein:
[0020] The plurality of trays are arranged on the inner side of the culture box and are equidistantly distributed in the height direction of the culture box, and are used to support the culture dish body, and the trays are provided with first air holes;
[0021] The culture box body is provided with a plurality of sliding grooves, and the sliding grooves are connected to the front side of the culture box body, and the tray is slidably connected to the sliding grooves.
[0022] On the basis of the above technical solution, preferably, it further comprises a plurality of limit baffles, wherein:
[0023] A plurality of limit baffles are respectively arranged on each of the trays, and the plurality of limit baffles on the tray are circumferentially equidistantly distributed, and the limit baffles include a guide portion and a supporting portion, wherein the guide portion and the supporting portion are both located on a side of the limit baffle close to the culture dish body, the side wall of the guide portion is an inclined wall, and the supporting portion is in contact with the peripheral wall of the culture dish body.
[0024] On the basis of the above technical solution, preferably, it also includes an ultraviolet sterilization lamp, wherein,
[0025] The ultraviolet sterilization lamp is arranged on one side of the top wall of the inner cavity of the culture box body, and is used for emitting ultraviolet rays to the inner cavity of the culture box body.
[0026] On the basis of the above technical solution, preferably, it further comprises a connecting pipe, a top cover and a breathable filter membrane, wherein:
[0027] The connecting pipe is connected to the top of the culture box;
[0028] The top cover is covered on the top of the connecting pipe, and a second through hole is opened on the top cover;
[0029] The air permeable filter membrane is arranged on the inner side of the second through hole and covers the second through hole.
[0030] On the basis of the above technical solution, preferably, it further comprises a sealing cover, wherein:
[0031] The inner side of the connecting pipe is provided with threads, and the sealing cover is inserted into the inner side of the connecting pipe and is threadedly connected with the connecting pipe.
[0032] The cell culture dish and storage culture box of the utility model have the following beneficial effects compared with the prior art:
[0033] (1) Specifically, when the culture dish body is being transported, the culture dish cover is placed on the top of the culture dish body in advance. At this time, the culture dish cover is engaged with the culture dish body through a snap-on structure, thereby completing the rapid positioning of the culture dish cover. At this time, the air filter can filter out the external air, preventing the cells stored in the culture dish body from contacting with the external air during the transportation process, thereby preventing the external air from contaminating the culture dish body, thereby increasing the quality assurance of the cells in the culture dish body when the culture dish body is transported externally.
[0034] (2) Specifically, when the culture dish bodies are stored inside the culture box, multiple culture dish bodies can be placed on top of multiple trays respectively. At this time, a culture dish body is placed on each tray, and the culture dish bodies are supported by the trays to prevent multiple trays from being stacked on top of each other, thereby preventing the support between the multiple trays from being unstable and causing tilting and spilling, which is convenient for use. BRIEF DESCRIPTION OF THE DRAWINGS
[0035] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.
[0036] Figure 1 A three-dimensional diagram of a cell culture dish of the present invention;
[0037] Figure 2 An exploded view of the cell culture dish of the utility model;
[0038] Figure 3 It is a schematic cross-sectional structure diagram of the cell culture dish of the utility model;
[0039] Figure 4 The cell culture dish of the utility model Figure 3 An enlarged schematic diagram of point A is shown;
[0040] Figure 5 A three-dimensional view of a storage incubator for cell culture dishes of the utility model;
[0041] Figure 6 It is a three-dimensional schematic diagram of the structure of the tray of the storage incubator of the cell culture dish of the utility model;
[0042] Figure 7 It is a three-dimensional schematic diagram of the structure of the connecting pipe of the storage culture box of the cell culture dish of the utility model;
[0043] Figure 8 The storage incubator for the cell culture dish of the utility model Figure 7 an exploded view of the structure shown;
[0044] Fig. 9 It is a schematic diagram of the connection method between the connecting pipe and the sealing cover of the storage culture box of the cell culture dish of the utility model;
[0045] Fig.10 This is a schematic diagram of the state of the sealing cover of the storage incubator for cell culture dishes of the present invention after the breathable cover is replaced. DETAILED DESCRIPTION
[0046] The following will be combined with the implementation of the utility model to clearly and completely describe the technical solutions in the implementation of the utility model. Obviously, the described implementation is only a part of the implementation of the utility model, not all of the implementations. Based on the implementation of the utility model, all other implementations obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.
[0047] like Figures 1 to 10 As shown, the cell culture dish and the storage culture box thereof of the utility model include a culture dish body 1, a culture dish cover 2, an air filter membrane 3 and a clamping component 4, wherein the culture dish cover 2 is detachably connected to the culture dish body 1 and covers the culture dish body 1, and a first through hole 21 connected to the culture dish body 1 is opened on the culture dish cover 2; the air filter membrane 3 is arranged on the inner side of the first through hole 21 and covers the first through hole 21; the clamping component 4 is arranged on the culture dish body 1 and is used to clamp the culture dish cover 2.
[0048] Specifically, when the culture dish body 1 is being transported, the culture dish cover 2 is covered on the top of the culture dish body 1 in advance. At this time, the culture dish cover 2 is engaged with the culture dish body 1 through the snap-fit structure, thereby completing the rapid positioning of the culture dish cover 2. At this time, the air filter 3 can filter out the external air to prevent the cells stored in the culture dish body 1 from contacting with the external air during the transportation process, thereby preventing the external air from contaminating the culture dish body 1, thereby increasing the protection of the cell quality in the culture dish body 1 when the culture dish body 1 is transported externally.
[0049] As a preferred embodiment, the culture dish body 1 includes an annular protrusion 11, which is located at the top of the culture dish body 1 and is inserted into the inner side of the culture dish cover 2 and fits with the inner wall of the culture dish cover 2. The clamping component 4 is arranged on the annular protrusion 11.
[0050] With this design, after the culture dish cover 2 is covered on the culture dish body 1 , the annular protrusion 11 presses against the culture dish cover 2 from the inner side of the culture dish cover 2 , thereby increasing the structural stability and the convenience of the structural design of the clamping component 4 .
[0051] As a preferred embodiment, the locking component 4 includes a locking ball 41 and a spring 42, wherein the annular protrusion 11 is provided with a plurality of mounting grooves 111, the locking ball 41 is slidably arranged on the inner side of the plurality of mounting grooves 111, and the inner wall of the culture dish cover 2 is provided with a locking groove 22 for the locking ball 41 to be inserted; the spring 42 is arranged between the locking ball 41 and the mounting groove 111, and is used to reset the sliding position of the locking ball 41.
[0052] By providing the locking ball 41 to be locked with the culture dish cover 2 , the locking ball 41 can be automatically locked with the culture dish cover 2 when the culture dish cover 2 is closed, thereby improving convenience and facilitating the removal of the culture dish cover 2 .
[0053] As a preferred embodiment, it further comprises a buckle plate 5 , wherein a plurality of buckle grooves 12 are opened on the peripheral side of the culture dish body 1 , and the buckle plate 5 is arranged on the inner side of the buckle groove 12 and a gap is formed between the buckle plate 5 and the buckle groove 12 .
[0054] Specifically, when opening the culture dish cover 2, one can insert one's fingers into the inner side of the buckle groove 12 to assist in opening the culture dish cover 2. Meanwhile, by providing the buckle plate 5, the culture dish body 1 can be conveniently lifted up by lifting the buckle plate 5 for transporting the culture dish body 1.
[0055] The present invention also proposes a storage incubator for cell culture dishes, comprising the above-mentioned cell culture dishes, and also comprising a culture box body 61, a transfer door 62 and a sealing insert ring 63, wherein the culture box body 61 is used to store the culture dish body 1, and the transfer door 62 is rotatably set on the culture box body 61 to cover the culture box body 61; the sealing insert ring 63 is a rubber insert ring, and when the transfer door 62 covers the culture box body 61, the sealing insert ring 63 is inserted into the inner side of the culture dish body 1 and fits with the inner wall of the culture dish body 1.
[0056] Specifically, when storing the culture dish body 1, the culture dish body 1 is placed inside the culture box 61 and rotated to cover the transfer door 62, thereby completing the storage of the culture dish body 1. By providing the sealing insert 63, when the transfer door 62 is closed, the sealing insert 63 can be synchronously inserted into the inside of the culture box 61 to increase the sealing performance.
[0057] It should be noted that when the transfer door 62 is opened or closed, it is performed in a clean bench to ensure that the gas in the chamber is sterile, thereby preventing contamination.
[0058] As a preferred embodiment, it also includes multiple trays 71, wherein the multiple trays 71 are all arranged on the inner side of the culture box body 61 and are equidistantly distributed in the height direction of the culture box body 61, for supporting the culture dish body 1, and a first air vent 711 is provided on the tray 71; the culture box body 61 is provided with multiple sliding grooves 611, and the sliding grooves 611 are connected to the front side of the culture box body 61, and the trays 71 are slidably connected to the sliding grooves 611.
[0059] With such a design, when the culture dish body 1 is stored inside the culture box 61, multiple culture dish bodies 1 can be placed on the top of multiple trays 71 respectively. At this time, a culture dish body 1 is placed on each tray 71, and the culture dish body 1 is supported by the tray 71 to prevent multiple trays 71 from being stacked on each other, thereby preventing unstable support between the multiple trays 71 and causing tilting and spilling problems, which is convenient to use.
[0060] As a preferred embodiment, it also includes a plurality of limit baffles 72, wherein the plurality of limit baffles 72 are respectively arranged on each tray 71, and the plurality of limit baffles 72 on the tray 71 are circumferentially equidistantly distributed, and the limit baffles 72 include a guide portion 721 and a supporting portion 722, wherein the guide portion 721 and the supporting portion 722 are both located on a side of the limit baffle 72 close to the culture dish body 1, the side wall of the guide portion 721 is an inclined wall, and the supporting portion 722 is in contact with the peripheral wall of the culture dish body 1.
[0061] In a specific implementation, the limiting baffle 72 is an arc-shaped piece. With this design, after the culture dish body 1 is placed on the tray 71, the multiple limiting baffles 72 can limit and block the culture dish body 1 from the surrounding side of the culture dish body 1, thereby increasing the stability of the culture dish body 1 when placed and preventing tilting and spilling. Specifically, when preventing the culture dish body 1 from tilting, the inclined wall of the guide part 721 can guide the culture dish body 1 between the multiple limiting baffles 72 for easy placement.
[0062] As a preferred embodiment, it further comprises an ultraviolet sterilization lamp 8 , wherein the ultraviolet sterilization lamp 8 is arranged on one side of the top wall of the inner cavity of the culture box 61 , and is used for emitting ultraviolet rays into the inner cavity of the culture box 61 .
[0063] In a specific implementation, the ultraviolet sterilization lamp 8 can be activated to sterilize and disinfect the interior of the culture box 61 to optimize the storage environment.
[0064] As a preferred embodiment, it also includes a connecting tube 91, a top cover 92 and a breathable filter membrane 10, wherein the connecting tube 91 is connected to the top of the culture box body 61; the top cover 92 covers the top of the connecting tube 91, and a second through hole 921 is opened on the top cover 92; the breathable filter membrane 10 is arranged on the inner side of the second through hole 921 and covers the second through hole 921.
[0065] The connecting tube 91 further comprises a sealing cover 93 , wherein the inner side of the connecting tube 91 is provided with threads, and the sealing cover 93 is inserted into the inner side of the connecting tube 91 and is threadedly connected with the connecting tube 91 .
[0066] With this design, the culture conditions of some cells do not require CO2, and the sealing cover 93 is closed on the connecting tube 91 to facilitate the storage of cells that do not require CO2 conditions.
[0067] In a specific implementation, the connecting tube 91 has two tube bodies, wherein the diameter of the tube body connected to the culture box body 61 is smaller than the diameter of the other tube body, and the sealing cover 93 is threadedly connected to the inside of the other tube body.
[0068] Preferably, the connecting pipe 91 includes a supporting portion 911 . Specifically, when the sealing cover 93 is connected, the supporting portion 911 is used to cover the sealing cover 93 .
[0069] Preferably, the sealing cover 93 may be replaced by a breathable cover 94 , which is threadedly connected to the inner wall of the connecting pipe 91 , and has a second breathable hole 941 .
[0070] Preferably, both the sealing cover 93 and the breathable cover 94 are provided with pull rings 95 for facilitating the adjustment of the rotation of the sealing cover 93 and the breathable cover 94 .
[0071] Preferably, a storage bin temperature control component is also provided inside the incubator body 61, and the storage bin temperature control component is used to heat the inside of the incubator body 61. When the incubator is frequently opened and the temperature is unstable, the storage bin temperature control component will automatically and quickly control the setting constant temperature.
[0072] The working principle of the utility model is introduced as follows:
[0073] After the cells are stored inside the culture dish body 1 in the clean bench, the culture dish cover 2 is covered on the top of the culture dish body 1, and the card ball 41 is inserted into the inside of the card slot 22 under the action of the spring 42, thereby completing the card connection and positioning processing of the culture dish cover 2. At this time, the air filter membrane 3 on the culture dish cover 2 can filter the air, thereby preventing the air from contaminating the cells during the external transportation process. In the clean bench, the culture dish body 1 is placed on the top of each tray 71 and located between multiple limit baffles 72 for storage. At this time, the tray 71 and the limit baffle 72 can increase the storage stability of the culture dish body 1. During storage, the ultraviolet sterilization lamp 8 can be turned on to disinfect the inside of the culture box 61. Usually, after each group of cell culture is completed, the ultraviolet sterilization storage bin is opened after the culture dish is taken out to avoid cross contamination caused by the next group of cells being placed in the bin. When it is necessary to store cells that do not require CO2 conditions, the sealing cover 93 will be covered on the connecting pipe 91 to facilitate the storage of cells that do not require CO2 conditions.
[0074] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.
Claims
1. A cell culture dish, Features: It comprises a culture dish body (1), a culture dish cover (2), an air filter membrane (3) and a clamping component (4), wherein: a culture dish cover (2) which is detachably connected to the culture dish body (1) and covers the culture dish body (1); the culture dish cover (2) is provided with a first through hole (21) which is in communication with the culture dish body (1); An air filter membrane (3) is arranged on the inner side of the first through hole (21) and covers the first through hole (21); A clamping component (4) is arranged on the culture dish body (1) and is used for clamping the culture dish cover (2).
2. The cell culture dish according to claim 1, Features: The culture dish body (1) comprises an annular protrusion (11), which is located at the top of the culture dish body (1) and is inserted into the inner side of the culture dish cover (2) and fits with the inner wall of the culture dish cover (2). The clamping component (4) is arranged on the annular protrusion (11).
3. The cell culture dish according to claim 2, Features: The clamping component (4) comprises a clamping ball (41) and a spring (42), wherein: The annular protrusion (11) is provided with a plurality of mounting grooves (111), the locking ball (41) is slidably arranged inside the plurality of mounting grooves (111), and the inner wall of the culture dish cover (2) is provided with a locking groove (22) for the locking ball (41) to be inserted into; A spring (42) is arranged between the locking ball (41) and the mounting groove (111) and is used to reset the sliding position of the locking ball (41).
4. The cell culture dish according to claim 1, Features: It also includes a gusset plate (5), wherein: A plurality of buckle grooves (12) are provided on the circumferential side of the culture dish body (1), and the buckle plates (5) are arranged inside the buckle grooves (12) and form gaps between the buckle plates (5) and the buckle grooves (12).
5. A storage incubator for cell culture dishes, Features: The cell culture dish comprises the cell culture dish as claimed in any one of claims 1 to 4, and further comprises a culture box (61), a transfer door (62) and a sealing insert (63), wherein: The culture box (61) is used to store the culture dish body (1), and the transfer door (62) is rotatably arranged on the culture box (61) to cover the culture box (61); The sealing insert ring (63) is a rubber insert ring. When the transfer door (62) covers the culture box body (61), the sealing insert ring (63) is inserted into the inner side of the culture dish body (1) and fits with the inner wall of the culture dish body (1).
6. The storage incubator for cell culture dishes according to claim 5, Features: Also included are a plurality of trays (71), wherein: The plurality of trays (71) are arranged on the inner side of the culture box (61) and are equidistantly distributed in the height direction of the culture box (61) for supporting the culture dish body (1), and the trays (71) are provided with first air holes (711); The culture box body (61) is provided with a plurality of sliding grooves (611), and the sliding grooves (611) are connected to the front side of the culture box body (61), and the tray (71) is slidably connected to the sliding grooves (611).
7. The storage incubator for cell culture dishes according to claim 6, Features: It also includes a plurality of limit stoppers (72), wherein: A plurality of limit blocking pieces (72) are respectively arranged on each of the trays (71); the plurality of limit blocking pieces (72) on the tray (71) are equidistantly distributed in the circumferential direction; the limit blocking piece (72) comprises a guide portion (721) and a support portion (722); wherein the guide portion (721) and the support portion (722) are both located on a side of the limit blocking piece (72) close to the culture dish body (1); the side wall of the guide portion (721) is an inclined wall, and the support portion (722) is in contact with the peripheral wall of the culture dish body (1).
8. The storage incubator for cell culture dishes according to claim 5, Features: It also includes an ultraviolet sterilization lamp (8), wherein: The ultraviolet sterilization lamp (8) is arranged on one side of the top wall of the inner cavity of the culture box (61) and is used to emit ultraviolet rays into the inner cavity of the culture box (61).
9. The storage incubator for cell culture dishes according to claim 5, Features: It also includes a connecting pipe (91), a top cover (92) and a breathable filter membrane (10), wherein: The connecting pipe (91) is connected to the top of the culture box (61); The top cover (92) covers the top of the connecting pipe (91), and a second through hole (921) is formed on the top cover (92); The air-permeable filter membrane (10) is arranged on the inner side of the second through hole (921) and covers the second through hole (921).
10. The storage incubator for cell culture dishes according to claim 9, Features: Also included is a sealing cover (93), wherein: The inner side of the connecting pipe (91) is provided with threads, and the sealing cover (93) is inserted into the inner side of the connecting pipe (91) and is threadedly connected to the connecting pipe (91).