Separation culture kit for hepatic sinus endothelial cells
By introducing refrigeration plate, sponge pad and fixing assembly into the hepatic sinusoidal endothelial cell isolation and culture kit, the problem of the kit lacking refrigeration function and test tube sliding is solved, cell activity and experimental stability are improved, and the effective refrigeration of test tubes is achieved and Anti-pollution.
Patent Information
- Application Number
- CN202420789648.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-04-16
- Publication Date
- 2024-12-17
- Estimated Expiration
- 2034-04-16
AI Technical Summary
The existing hepatic sinusoidal endothelial cell isolation and culture kit lacks the refrigeration function, resulting in short storage time of the reagent, affecting the experimental results, and the test tubes are prone to slide during transportation, affecting the stability of the experimental results.
A hepatic sinusoidal endothelial cell isolation and culture kit including a reagent box and a fixing component is designed. The reagent box is equipped with multiple tanks and breathable holes, and is equipped with a refrigeration plate and a sponge pad to refrigerate the test tube, and passes through the external threaded part with The connection of the internal threaded part and the engagement structure of the bumps and grooves fix the test tube to prevent tilt and contamination.
The refrigeration function of the refrigeration plate improves the activity and yield of the hepatic sinusoidal endothelial cells. The sponge pad keeps the test tube dry, the connection between the external thread part and the internal thread part prevents contamination, the fixed structure of the bumps and grooves prevents the test tube from tilting, the controller Maintain a constant temperature state and reduce the impact of external temperature on cells.
Smart Images
Figure CN222182275U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of separation and culture of hepatic sinusoidal endothelial cells, in particular to a kit for separation and culture of hepatic sinusoidal endothelial cells. Background Art
[0002] Sinusoidal endothelial cells are the most numerous non-parenchymal cells in the liver. Their role in liver microcirculation, liver regeneration, ischemia-reperfusion injury in liver transplantation, and the induction of transplantation immune tolerance has attracted the attention of researchers. The primary isolation and culture of high-purity and high-viability sinusoidal endothelial cells is the basis for studying their mechanism of action in various pathological and physiological environments of the liver. At present, the isolation and culture of sinusoidal endothelial cells need to be placed in the corresponding test kit, but the existing test kits usually do not have refrigeration function. At the same time, during transportation, the test tubes are prone to slipping in the test kit, thus affecting the results of the experiment.
[0003] Chinese patent CN205420442U discloses a liver sinusoidal endothelial cell isolation and culture kit, including a box body and four sterile sealed bottles arranged in the box body, wherein the four sterile sealed bottles are respectively filled with a cleaning concentrate, a digestion solution, a separation solution, and a culture solution for the isolation and culture of liver sinusoidal endothelial cells. The kit contains all reagents required for the isolation and culture of liver sinusoidal endothelial cells, and no additional reagent preparation is required, and the experiment is convenient and fast; all reagent bottles are sterile sealed bottles, and no sterilization is required, and the operation is simple.
[0004] When storing the hepatic sinusoidal endothelial cells in this patent, since the kit does not have a refrigeration function, the storage time of the reagent is short, which affects the subsequent experimental results. Therefore, we propose a hepatic sinusoidal endothelial cell isolation and culture kit. Utility Model Content
[0005] The purpose of the utility model is to provide a liver sinusoidal endothelial cell isolation and culture kit in order to solve the above problems, thereby solving the problems mentioned in the background technology.
[0006] In order to solve the above problems, the utility model provides a technical solution:
[0007] A hepatic sinusoidal endothelial cell isolation and culture kit comprises a reagent box and a fixing component, wherein the reagent box is provided with a plurality of slots, the inner wall of each of the slots is provided with a plurality of air holes, a refrigeration plate is fixedly connected to the interior of the reagent box, a test tube is detachably connected to the interior of each of the slots, a sponge pad is placed inside each of the slots, the bottom of each of the test tubes contacts the top of the corresponding sponge pad, and the fixing component is arranged outside the test tube.
[0008] As a preferred solution of the utility model, the fixing assembly includes a ferrule, each of the ferrules is fixedly connected to the outer wall of the corresponding test tube, and the bottom of each of the ferrules is symmetrically fixedly connected with two protrusions.
[0009] As a preferred solution of the utility model, a plurality of grooves 1 are provided on the top of the reagent box, and the bottom of each of the protrusions extends to the inside of the corresponding groove 1.
[0010] As a preferred solution of the utility model, the outer wall of each test tube is fixedly connected with an external threaded portion, the top of each test tube is detachably connected with a cap, and the interior of each cap is fixedly connected with an internal threaded portion used in conjunction with the external threaded portion.
[0011] As a preferred solution of the utility model, a cover plate is rotatably connected to the top of the reagent box, and a plurality of grooves 2 are provided inside the cover plate.
[0012] As a preferred solution of the utility model, a controller is fixedly connected to one side of the reagent box, and the controller is electrically connected to the refrigeration plate.
[0013] The beneficial effects of the utility model are as follows: by arranging a refrigeration plate inside the reagent box, cold air can pass through the air holes to refrigerate and preserve the test tubes, thereby improving the activity and yield of the hepatic sinusoidal endothelial cells; by placing a sponge pad inside the groove body, water vapor outside the test tube can be absorbed, so that the outer wall of the test tube remains dry; by the threaded connection between the external threaded part and the internal threaded part, the cap can be covered on the top of the test tube to prevent the external environment from polluting the test tube; by the snap-fit connection between the protrusion and the groove one, the test tube can be fixed inside the corresponding groove body to prevent the test tube from tilting during transportation; by controlling the temperature of the refrigeration plate by the controller, the hepatic sinusoidal endothelial cells inside the test tube can always be in a constant temperature state, reducing the influence of the external temperature on the hepatic sinusoidal endothelial cells. BRIEF DESCRIPTION OF THE DRAWINGS
[0014] For ease of explanation, the present invention is described in detail by the following specific implementations and drawings.
[0015] Figure 1 It is a schematic diagram of the appearance of the utility model;
[0016] Figure 2 It is a schematic internal cross-sectional view of the utility model;
[0017] Figure 3 This utility model Figure 2 The enlarged view of point A in the middle;
[0018] Figure 4 It is a schematic diagram of the appearance of the cover in the utility model.
[0019] In the figure: 1. reagent box; 2. tank body; 3. test tube; 4. external threaded portion; 5. air vent; 6. sponge pad; 7. fixing assembly; 71. ring; 72. bump; 73. groove one; 8. cooling plate; 9. cap; 10. internal threaded portion; 11. cover plate; 12. groove two; 13. controller. DETAILED DESCRIPTION
[0020] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the accompanying drawings in the embodiments of the present invention.
[0021] Example
[0022] See also Figure 1-Figure 4 The utility model provides a technical solution: a hepatic sinusoidal endothelial cell isolation and culture kit, comprising a reagent box 1 and a fixing component 7, wherein a plurality of slots 2 are provided inside the reagent box 1, and a plurality of air holes 5 are provided on the inner wall of each slot 2, and a refrigeration plate 8 is fixedly connected to the inside of the reagent box 1, and by arranging the refrigeration plate 8 inside the reagent box 1, cold air can pass through the air holes 5 to refrigerate and preserve the test tubes 3, thereby improving the activity and yield of hepatic sinusoidal endothelial cells; a test tube 3 is detachably connected to the inside of each slot 2, and a sponge pad 6 is placed inside each slot 2, and the bottom of each test tube 3 contacts the top of the corresponding sponge pad 6, and by placing the sponge pad 6 inside the slot 2, the water vapor outside the test tube 3 can be absorbed, so that the outer wall of the test tube 3 remains dry; the fixing component 7 is provided It is placed on the outside of the test tube 3, and is characterized in that the outer wall of each test tube 3 is fixedly connected with an external threaded portion 4, the top of each test tube 3 is detachably connected with a cap 9, the inside of each cap 9 is fixedly connected with an internal threaded portion 10 used in conjunction with the external threaded portion 4, and the threaded connection between the external threaded portion 4 and the internal threaded portion 10 allows the cap 9 to cover the top of the test tube 3 to prevent the external environment from contaminating the test tube 3; the top of the reagent box 1 is rotatably connected with a cover plate 11, and a plurality of grooves 12 are provided inside the cover plate 11. A controller 13 is fixedly connected to one side of the reagent box 1, and the controller 13 is electrically connected to the refrigeration plate 8. The temperature of the refrigeration plate 8 is controlled by the controller 13, so that the hepatic sinusoidal endothelial cells inside the test tube 3 can always be in a constant temperature state, reducing the influence of the external temperature on the hepatic sinusoidal endothelial cells.
[0023] Furthermore, the fixing assembly 7 includes a ring 71, each ring 71 is fixedly connected to the outer wall of the corresponding test tube 3, and the bottom of each ring 71 is symmetrically fixedly connected to two protrusions 72. The top of the reagent box 1 is provided with a plurality of grooves 73, and the bottom of each protrusion 72 extends to the inside of the corresponding groove 73. Through the snap-fit connection between the protrusion 72 and the groove 73, the test tube 3 can be fixed inside the corresponding trough body 2 to prevent the test tube 3 from tilting during transportation.
[0024] To sum up: first place the sponge pad 6 in order inside the slot body 2, then start the controller 13, the controller 13 controls the temperature of the refrigeration plate 8, and then place the test tube 3 inside the slot body 2. In order to prevent the test tube 3 from shaking and tilting, press the ring 71, the ring 71 drives the protrusion 72 to extend into the inside of the groove 73, and realize the engagement with the groove 73. At the same time, rotate the test tube cover 9 so that the top of each test tube 3 is sealed to prevent the external environment from polluting the inside of the test tube 3. Finally, rotate the cover plate 11 so that the top of the reagent box 1 is covered. It should be noted that the staff chooses to replace the sponge pad 6 according to the situation.
[0025] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.
Claims
1. A kit for isolating and culturing hepatic sinusoidal endothelial cells, characterized in that: The invention comprises a reagent box (1) and a fixing component (7), wherein a plurality of slots (2) are provided inside the reagent box (1), the inner wall of each slot (2) is provided with a plurality of air holes (5), a refrigeration plate (8) is fixedly connected inside the reagent box (1), a test tube (3) is detachably connected inside each slot (2), a sponge pad (6) is placed inside each slot (2), the bottom of each test tube (3) is in contact with the top of the corresponding sponge pad (6), and the fixing component (7) is arranged outside the test tube (3).
2. A kit for isolating and culturing hepatic sinusoidal endothelial cells according to claim 1, characterized in that: The fixing assembly (7) comprises a ferrule (71), each of the ferrules (71) being fixedly connected to the outer wall of the corresponding test tube (3), and the bottom of each of the ferrules (71) being symmetrically fixedly connected to two protrusions (72).
3. A kit for isolating and culturing hepatic sinusoidal endothelial cells according to claim 2, characterized in that: The top of the reagent box (1) is provided with a plurality of grooves (73), and the bottom of each protrusion (72) extends into the interior of the corresponding groove (73).
4. A kit for isolating and culturing hepatic sinusoidal endothelial cells according to claim 1, characterized in that: The outer wall of each test tube (3) is fixedly connected to an external threaded portion (4), the top of each test tube (3) is detachably connected to a cap (9), and the interior of each cap (9) is fixedly connected to an internal threaded portion (10) used in conjunction with the external threaded portion (4).
5. A kit for isolating and culturing hepatic sinusoidal endothelial cells according to claim 1, characterized in that: The top of the reagent box (1) is rotatably connected to a cover plate (11), and a plurality of grooves (12) are provided inside the cover plate (11).
6. A kit for isolating and culturing hepatic sinusoidal endothelial cells according to claim 1, characterized in that: A controller (13) is fixedly connected to one side of the reagent box (1), and the controller (13) is electrically connected to the refrigeration plate (8).