Cell culture device capable of sealing test

Abstract

The utility model discloses a cell culture device capable of testing in a sealing manner. The cell culture device comprises a culture device, a first material supplementing bottle, a second material supplementing bottle and a tail gas bottle, the first gas pipeline and the second gas pipeline are connected in a sealing manner, the gas inlet pipeline is provided with a first switch, the second exhaust pipeline is provided with a second switch, the tail gas bottle can be used for containing sealing test liquid, one end of the first exhaust pipeline extends to the bottom of the tail gas bottle, and one end of the second exhaust pipeline is connected with the upper end of the tail gas bottle. If the cell culture device has the problem of poor sealing, the liquid level height in the sealing test liquid in the tail gas bottle is changed, and if the cell culture device has good sealing performance, the liquid level height in the sealing test liquid in the tail gas bottle is kept unchanged, that is, the cell culture device can realize a sealable test by adding the sealing test liquid; compared with the mode that the whole device is placed at the water bottom for testing, one person can complete the sealing test of the cell culture device, and the sealing performance is judged more accurately through the height of the liquid level.

Description

Technical Field

[0001] The utility model relates to the technical field of biochemical culture, in particular to a cell culture device capable of sealing testing. Background Art

[0002] The 3L reaction culture of the cell culture device needs to ensure that all pipes and joints are sealed after sterilization, and the 3L reactor needs to be tested for sealing before moist heat sterilization. The operator cannot identify tiny damage to the pipes and joints of the cell culture device with the naked eye. If there is damage that destroys the sealing of the cell culture device, it will be contaminated by microorganisms during the cell culture process, causing the culture batch to fail.

[0003] In the prior art, in order to implement a sealing test of a cell culture device, all inlets and outlets of the entire cell culture device are first sealed, and then the entire device is placed underwater to determine whether the sealing of the cell culture device is damaged by checking whether bubbles emerge. Since the cell culture device includes a reactor and multiple bottles, multiple people are required to operate simultaneously, and bubbles are also generated during manual pressing, it is easy to misjudge the sealing of the cell culture device by checking the bubbles. Utility Model Content

[0004] The utility model provides a cell culture device capable of sealing test, which is used to solve the problems of complicated operation and inaccurate test of the sealing test of the cell culture device.

[0005] In one embodiment, a sealable testable cell culture device is provided, comprising:

[0006] A culture vessel, wherein the culture vessel has a culture chamber for culturing cells, the culture vessel is connected to an air intake pipe and a first exhaust pipe, and the air intake pipe is provided with a first switch;

[0007] A first feeding bottle, wherein the first feeding bottle is connected to a first gas pipeline and a first feeding pipeline, and the first feeding pipeline is connected to the culture vessel;

[0008] A second feeding bottle, wherein the second feeding bottle is connected to a second gas pipeline and a second feeding pipeline, the second feeding pipeline is connected to the culture vessel, and the first feeding pipeline and the second feeding pipeline are sealably connected; and

[0009] The tail gas bottle can be used to accommodate sealing test liquid, the first exhaust pipe is connected to the tail gas bottle, and one end of the first exhaust pipe extends to the bottom of the tail gas bottle, the tail gas bottle is connected to the second exhaust pipe, one end of the second exhaust pipe is connected to the upper end of the tail gas bottle, and the second exhaust pipe is provided with a second switch.

[0010] In one embodiment, a sealing test liquid is provided in the tail gas bottle, the sealing test liquid submerges one end of the first exhaust pipe extending into the tail gas bottle, and the sealing test liquid does not submerge one end of the second exhaust pipe connected to the tail gas bottle.

[0011] In one embodiment, the tail gas bottle is a transparent or translucent structure, or the color of the sealing test liquid is different from the color of the tail gas bottle.

[0012] In one embodiment, the first exhaust duct is a transparent or translucent structure.

[0013] In one embodiment, the sealing test liquid is pure water.

[0014] In one embodiment, the first switch is a first clamp clamped on the outside of the intake duct, and / or the second switch is a second clamp clamped on the outside of the second exhaust duct.

[0015] In one embodiment, the first switch is a first valve disposed inside the intake duct, and / or the second switch is a second valve disposed inside the second exhaust duct.

[0016] In one embodiment, the air intake pipeline, the first exhaust pipeline, the second exhaust pipeline, the first gas pipeline and the second gas pipeline are respectively connected to air filters.

[0017] In one embodiment, the second exhaust duct is connected to a first branch exhaust duct and a second branch exhaust duct, and the first branch exhaust duct and the second branch exhaust duct are respectively connected to air filters.

[0018] In one embodiment, it further comprises an air source, and one end of the air inlet pipe away from the culture vessel is detachably connected to the air source.

[0019] According to the sealable testable cell culture device of the above-mentioned embodiment, since the first gas pipeline of the first feeding bottle and the second gas pipeline of the second feeding bottle can be sealably connected, the air inlet pipeline is provided with a first switch, and the second exhaust pipeline is provided with a second switch, so that the cell culture device can form a sealing structure, and the tail gas bottle can be used to accommodate the sealing test liquid, one end of the first exhaust pipeline extends to the bottom of the tail gas bottle, and one end of the second exhaust pipeline is connected to the upper end of the tail gas bottle. The sealing test liquid in the tail gas bottle can separate the closed cavity formed in the incubator, the first feeding bottle and the second feeding bottle from the closed cavity in the tail gas bottle. If the cell culture device has a poor sealing problem, the liquid level height of the sealing test liquid in the tail gas bottle will change. If the cell culture device has good sealing performance, the liquid level height of the sealing test liquid in the tail gas bottle will remain unchanged. That is, the present cell culture device can achieve a sealable test by adding a sealing test liquid. Compared with placing the entire device under water for testing, one person can complete the sealing test of the present cell culture device, and it is more accurate to judge the sealing performance by the liquid level height. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 A schematic diagram of the structure of a cell culture device in an embodiment;

[0021] Figure 2 A schematic diagram of the structure of a cell culture device in a test state in an embodiment;

[0022] Figure 3 A schematic diagram of the structure of a cell culture device in a test state in an embodiment;

[0023] Figure 4 A schematic diagram of the structure of a cell culture device in a test state in an embodiment;

[0024] The reference numerals are as follows:

[0025] 1-culture device, 1a-culture chamber, 11-air inlet pipe, 12-first exhaust pipe, 13-first switch;

[0026] 2-first feeding bottle, 21-first gas pipeline, 22-first feeding pipeline;

[0027] 3-second feeding bottle, 31-second gas pipeline, 32-second feeding pipeline;

[0028] 4- tail gas bottle, 41- second exhaust pipe, 42- second switch, 43- first branch exhaust pipe, 44-;

[0029] 5-Seal test liquid;

[0030] 6- Air filter. DETAILED DESCRIPTION

[0031] The present invention is further described in detail below by specific embodiments in conjunction with the accompanying drawings. Wherein similar elements in different embodiments adopt associated similar element numbers. In the following embodiments, many detailed descriptions are intended to enable the present application to be better understood. However, those skilled in the art can easily recognize that some of the features can be omitted in different situations, or can be replaced by other elements, materials, and methods. In some cases, some operations related to the present application are not shown or described in the specification, in order to avoid the core part of the present application being overwhelmed by too much description, and for those skilled in the art, it is not necessary to describe these related operations in detail, and they can fully understand the related operations based on the description in the specification and the general technical knowledge in the art.

[0032] In addition, the features, operations or characteristics described in the specification can be combined in any appropriate manner to form various implementations. At the same time, the steps or actions in the method description can also be interchanged or adjusted in a manner that is obvious to those skilled in the art. Therefore, the various sequences in the specification and the drawings are only for the purpose of clearly describing a certain embodiment and are not meant to be a required sequence, unless otherwise specified that a certain sequence must be followed.

[0033] The serial numbers of the components in this document, such as "first", "second", etc., are only used to distinguish the objects described and do not have any order or technical meaning. The "connection" and "coupling" mentioned in this application, unless otherwise specified, include direct and indirect connections (couplings).

[0034] In one embodiment, a cell culture device capable of sealing test is provided. The cell culture device can realize sealing test. The sealing test is performed before cell culture, and the sealing test is not performed simultaneously with cell culture. After the cell culture device is confirmed to have no damage that affects the sealing performance through the sealing test, it is sterilized by moist heat before cell culture is performed.

[0035] Please refer to Figure 1 and Figure 2The cell culture device of this embodiment mainly includes a culture vessel 1, a first feeding bottle 2, a second feeding bottle 3 and a tail gas bottle 4. The culture vessel 1 is used to place biochemical samples such as cells, and the culture vessel 1 is also used to provide a place for cell culture. The first feeding bottle 2 and the second feeding bottle 3 are respectively connected to the culture vessel 1, and the first feeding bottle 2 and the second feeding bottle 3 are respectively used to supplement the nutrients required for cell culture in the culture vessel 1. The tail gas bottle 4 is connected to the culture vessel 1, and the tail gas bottle 4 is used to collect and discharge the waste gas generated during the cell culture process in the culture vessel 1. This cell culture device can realize the test of the sealing of the culture vessel 1, the first feeding bottle 2, the second feeding bottle 3 and the tail gas bottle 4, which includes the sealing of the respective cavities of the culture vessel 1, the first feeding bottle 2, the second feeding bottle 3 and the tail gas bottle 4 and the sealing of the connection between each other.

[0036] The culture vessel 1, the first feeding bottle 2, the second feeding bottle 3 and the tail gas bottle 4 all include a detachable cover, which forms a sealed cavity structure when the cover is closed, and the corresponding substance can be added through the detachable cover.

[0037] The culture vessel 1 includes a culture chamber 1a, which is used to place cells and provide a place for cell culture. The culture vessel 1 is connected with an air intake pipe 11 and a first exhaust pipe 12, one end of the air intake pipe 11 extends to the bottom of the culture vessel 1, and the other end of the air intake pipe 11 is located outside the culture vessel 1. The air intake pipe 11 is used to introduce gas required for culture into the culture chamber 1a, such as sterile oxygen, carbon dioxide or air.

[0038] The air inlet pipeline 11 is also provided with a first switch 13, which can be used to open and close the air inlet pipeline 11. When the first switch 13 opens the air inlet pipeline 11, the culture chamber 1a can be connected with the outside through the air inlet pipeline 11 to inject gas into the culture chamber 1a; when the first switch 13 closes the air inlet pipeline 11, the air inlet of the culture chamber 1a is closed, and gas cannot be injected into the culture chamber 1a.

[0039] One end of the first exhaust duct 12 is connected to the upper end of the culture vessel 1, that is, one end of the first exhaust duct 12 is connected to the upper end of the culture chamber 1a, and the other end of the first exhaust duct 12 is connected to the tail gas bottle 4. The first exhaust duct 12 connects the culture vessel 1 and the tail gas bottle 4, so that the gas generated in the culture vessel 1 can be discharged into the tail gas bottle 4.

[0040] The first feeding bottle 2 has a container for storing nutrients, and the first feeding bottle 2 is connected to a first gas pipeline 21 and a first feeding pipeline 22. The first gas pipeline 22 extends into the bottom of the container and is connected to the material source. The first gas pipeline 21 is away from the bottom of the container and is externally connected to an air filter 6. During the cultivation process, a peristaltic pump is used to inject the nutrients in the feeding bottle 2 into the culture vessel 1 through the first feeding pipeline 22.

[0041] The second feeding bottle 3 has a container for storing nutrients, and the second feeding bottle 3 is connected to a second gas pipeline 31 and a second feeding pipeline 32. The second gas pipeline 32 extends into the bottom of the container and is connected to the material source. The second gas pipeline 31 is far away from the bottom of the container and is externally connected to an air filter 6. During the cultivation process, a peristaltic pump is used to inject the nutrients in the feeding bottle 3 into the culture vessel 1 through the second feeding pipeline 32.

[0042] The first gas pipeline 21 and air filter 6 of the first feeding bottle 2 and the second gas pipeline 31 and air filter 6 of the second feeding bottle 3 are sealedly connected by sealing parts such as sealing pipes, so that the culture vessel 1, the first feeding bottle 2 and the second feeding bottle 3 are connected in series to form a cavity.

[0043] The tail gas bottle 4 has a cavity for accommodating exhaust gas, and the cavity can also be used to accommodate the sealing test liquid 5. The tail gas bottle 4 is connected to the first exhaust pipe 12, and one end of the first exhaust pipe 12 extends to the bottom of the tail gas bottle 4. The tail gas bottle 4 is connected to the second exhaust pipe 41, one end of the second exhaust pipe 41 is connected to the upper end of the tail gas bottle 4, and the other end of the second exhaust pipe 41 is connected to the atmosphere. The tail gas bottle 4 can discharge the collected exhaust gas into the atmosphere through the second exhaust pipe 41.

[0044] The second exhaust pipe 41 is provided with a second switch 42, which can be used to open and close the intake pipe 11. When the second switch 42 opens the second exhaust pipe 41, the tail gas bottle 4 can be connected to the outside through the second exhaust pipe 41 to discharge the tail gas to the atmosphere; when the second switch 42 closes the second exhaust pipe 41, the exhaust of the tail gas bottle 4 is closed, and the gas cannot be discharged to the atmosphere.

[0045] The principle and steps of the sealing test of the cell culture device of this embodiment are as follows:

[0046] (1) Seal and connect the end of the first gas pipeline 21 away from the first feeding bottle 2 and the end of the second gas pipeline 31 away from the second feeding bottle 3. Please refer to Figure 2 ;

[0047] (2) injecting the sealing test liquid 5 into the tail gas bottle 4, the sealing test liquid 5 submerging the first exhaust pipe 12 extending to one end of the tail gas bottle 4, and the sealing test liquid 5 not submerging the end of the second exhaust pipe 41 connected to the tail gas bottle 4; and opening the first switch 13 and the second switch 42;

[0048] The first step and the second step can be performed simultaneously, or the second step can be performed first and then the first step.

[0049] (3) The end of the air inlet pipe 11 away from the culture vessel 1 is connected to the air source, and the air source injects gas into the culture vessel 1 to maintain the compressed air pressure at 0.05 MPa;

[0050] (4) When bubbles appear in the tail gas bottle 4, close the second switch 42 and continue to inject gas into the culture vessel 1;

[0051] The bubbles in the tail gas bottle 4 come from the air discharged from the culture vessel 1 into the tail gas bottle 4. When there are bubbles in the tail gas bottle 4, it means that the culture vessel 1, the first feeding bottle 2 and the second feeding bottle 3 are being filled with gas.

[0052] (5) When there are no bubbles in the tail gas bottle 4, it means that the incubator 1, the first feeding bottle 2 and the second feeding bottle 3 are filled with gas, and the first switch 13 is closed;

[0053] Among them, when there are no bubbles in the tail gas bottle 4, it means that the air pressure in the cavity formed by the culture vessel 1, the first feeding bottle 2 and the second feeding bottle 3 is balanced with the air pressure in the tail gas bottle 4.

[0054] (6) Keep it still for 5 to 10 minutes, and observe the change in the liquid level of the sealing test liquid 5 in the tail gas bottle 4; if part of the sealing test liquid 5 is absorbed by the incubator 1, the liquid level of the sealing test liquid 5 in the tail gas bottle 4 will decrease, which means that there is leakage in the cavity formed by the incubator 1, the first feeding bottle 2 and the second feeding bottle 3, that is, the incubator 1, the first feeding bottle 2 and the second feeding bottle 3 and their connections need to be reconnected; if the liquid level of the sealing test liquid 5 in the tail gas bottle 4 remains unchanged, it means that the cavity formed by the incubator 1, the first feeding bottle 2 and the second feeding bottle 3 is well sealed and there is no damage that destroys the sealing.

[0055] The cell culture device of this embodiment, since the first gas pipeline 21 of the first feeding bottle 2 and the second gas pipeline 31 of the second feeding bottle 3 can be sealed and connected, the air inlet pipeline 11 is provided with a first switch 13, and the second exhaust pipeline 41 is provided with a second switch 42, so that the cell culture device can form a sealed structure, and the tail gas bottle 4 can be used to accommodate the sealing test liquid, one end of the first exhaust pipeline 12 extends to the bottom of the tail gas bottle 4, and one end of the second exhaust pipeline 41 is connected to the upper end of the tail gas bottle 4. The sealing test liquid 5 in the tail gas bottle 4 can separate the closed cavity formed in the culture vessel 1, the first feeding bottle 2 and the second feeding bottle 3 from the closed cavity in the tail gas bottle 4. If the cell culture device has a poor sealing problem, the liquid level height in the sealing test liquid in the tail gas bottle 4 will change. If the cell culture device has good sealing performance, the liquid level height in the sealing test liquid in the tail gas bottle 4 will remain unchanged. That is, the cell culture device can achieve a sealable test by adding a sealing test liquid. Compared with placing the entire device under water for testing, one person can complete the sealing test of the cell culture device, and it is more accurate to judge the sealing performance by the liquid level height.

[0056] In one embodiment, the tail gas bottle 4 may be pre-filled with a sealing test liquid 5. After the sealing test is completed, the sealing test liquid 5 is discharged, and the whole is sterilized before cell culture.

[0057] Among them, the tail gas bottle 4 contains a sealing test liquid 5 with half its volume, that is, the upper half of the tail gas bottle 4 still contains air, and the sealing test liquid 5 submerges the first exhaust pipe 12 extending to one end of the bottom of the tail gas bottle 4, and the sealing test liquid 5 does not submerge the second exhaust pipe 41 and one end of the top of the tail gas bottle 4. The sealing test liquid 5 separates the cavity in the incubator 1 from the cavity of the air part in the tail gas bottle 4.

[0058] In one embodiment, the tail gas bottle 4 can be a transparent or translucent structure, or the tail gas bottle 4 can be provided with a transparent window. The tail gas bottle 4 is set to a transparent structure, which can facilitate the staff to observe the liquid level of the sealed test liquid 5 in the tail gas bottle 4.

[0059] The sealing test liquid 5 can be pure water, which is sterilized and will not pollute the cell culture device. The sealing test liquid 5 can also be other sterilized liquids, such as distilled water.

[0060] In one embodiment, the color of the sealing test liquid 5 can be different from the color of the tail gas bottle 4. For example, the tail gas bottle 4 is transparent white, and the sealing test liquid 5 is red or yellow. The color of the sealing test liquid 5 can more clearly observe the change in the liquid level of the sealing test liquid 5.

[0061] In one embodiment, the first exhaust pipe 12 is a transparent or translucent structure, and the change in the liquid level of the sealing test liquid 5 is caused by the first exhaust pipe 12 sucking liquid, so the sealing of the cell culture device can also be judged by observing whether there is liquid in the first exhaust pipe 12. In addition, the change in the height of the liquid in the first exhaust pipe 12 is much greater than the change in the liquid level of the sealing test liquid 5 in the tail gas bottle 4. Therefore, the transparent structure of the first exhaust pipe 12 is more convenient for observing and judging the sealing of the cell culture device.

[0062] In one embodiment, the first switch 13 may be a first clip, which is clamped on the outer side of the air intake duct 11. When the first clip is removed, the air intake duct 11 is opened, and when the first clip is clamped on the air intake duct 11, the air intake duct 11 is closed.

[0063] The first switch 13 may also be a switch such as a first valve disposed in the air intake pipe 11 , which can also realize the opening and closing control of the air intake pipe 11 .

[0064] In one embodiment, the second switch 42 may be a second clamp, which is clamped on the outer side of the second exhaust duct 41. When the second clamp is removed, the second exhaust duct 41 is opened, and when the second clamp is clamped on the second exhaust duct 41, the second exhaust duct 41 is closed.

[0065] The second switch 42 may also be a switch such as a second valve disposed in the second exhaust duct 41 , and may also realize the opening and closing control of the second exhaust duct 41 .

[0066] Please refer to Figure 3 In one embodiment, the air intake duct 11, the first exhaust duct 12, the second exhaust duct 41, the first gas duct 21 and the second gas duct 31 are respectively connected to an air filter 6, and the air filter 6 can filter other air entering or discharged to avoid contaminating the cell culture device or polluting the atmosphere.

[0067] The air filter 6 is a 0.2 μm air filter, which can filter substances with a diameter greater than 0.2 μm.

[0068] Please refer to Figure 4 In one embodiment, the second exhaust duct 41 is connected to the first branch exhaust duct 43 and the second branch exhaust duct 44, and the first branch exhaust duct 43 and the second branch exhaust duct 44 are respectively connected to the air filter 6. The second exhaust duct 41 is provided with a branch duct, so that when the air filter 6 of one of the first branch exhaust ducts 43 is blocked, the second branch exhaust duct 44 and the air filter 6 can be enabled for exhaust, avoiding the problem of air filter 6 being blocked and unable to exhaust.

[0069] In one embodiment, the cell culture device may further include an air source, and the end of the air inlet pipe 11 away from the culture vessel 1 is detachably connected to the air source. The air source may be a compressed air device, and the air source is used to provide compressed air to the cell culture device to achieve a sealing test.

[0070] The above specific examples are used to illustrate the present invention, which are only used to help understand the present invention and are not intended to limit the present invention. For those skilled in the art of the present invention, some simple deductions, deformations or substitutions can be made based on the idea of ​​the present invention.

Claims

1. A sealable testable cell culture device, characterized in that: include: A culture vessel, wherein the culture vessel has a culture chamber for culturing cells, the culture vessel is connected to an air intake pipe and a first exhaust pipe, and the air intake pipe is provided with a first switch; A first feeding bottle, wherein the first feeding bottle is connected to a first gas pipeline and a first feeding pipeline, and the first feeding pipeline is connected to the culture vessel; A second feeding bottle, wherein the second feeding bottle is connected to a second gas pipeline and a second feeding pipeline, the second feeding pipeline is connected to the culture vessel, and the first feeding pipeline and the second feeding pipeline are sealably connected; and The tail gas bottle can be used to accommodate sealing test liquid, the first exhaust pipe is connected to the tail gas bottle, and one end of the first exhaust pipe extends to the bottom of the tail gas bottle, the tail gas bottle is connected to the second exhaust pipe, one end of the second exhaust pipe is connected to the upper end of the tail gas bottle, and the second exhaust pipe is provided with a second switch.

2. The sealable testable cell culture device according to claim 1, characterized in that: The tail gas bottle is provided with a sealing test liquid, the sealing test liquid submerges one end of the first exhaust pipe extending into the tail gas bottle, and the sealing test liquid does not submerge one end of the second exhaust pipe connected to the tail gas bottle.

3. The sealable testable cell culture device according to claim 2, characterized in that: The tail gas bottle is a transparent or translucent structure, or the color of the sealing test liquid is different from the color of the tail gas bottle.

4. The sealable testable cell culture device according to claim 3, characterized in that: The first exhaust duct is a transparent or semi-transparent structure.

5. The sealable testable cell culture device according to claim 2, characterized in that: The sealing test liquid is pure water.

6. The sealable testable cell culture device according to claim 1, characterized in that: The first switch is a first clamp clamped on the outside of the intake duct, and / or the second switch is a second clamp clamped on the outside of the second exhaust duct.

7. The sealable testable cell culture device according to claim 1, characterized in that: The first switch is a first valve disposed inside the intake pipe, and / or the second switch is a second valve disposed inside the second exhaust pipe.

8. The sealable testable cell culture device according to claim 1, characterized in that: The air intake pipeline, the first exhaust pipeline, the second exhaust pipeline, the first gas pipeline and the second gas pipeline are respectively connected with air filters.

9. The sealable testable cell culture device according to claim 1, characterized in that: The second exhaust duct is connected to a first branch exhaust duct and a second branch exhaust duct, and the first branch exhaust duct and the second branch exhaust duct are respectively connected to air filters.

10. The sealable testable cell culture device according to claim 1, characterized in that: It also includes an air source, and one end of the air inlet pipe away from the culture vessel is detachably connected to the air source.

Images