Nucleic acid extraction and pipetting integrated device and nucleic acid extraction and pipetting system

Abstract

The utility model relates to a nucleic acid extraction and pipetting integrated device and a nucleic acid extraction and pipetting system.The nucleic acid extraction and pipetting integrated device comprises a sleeve, a piston rod, an extraction sleeve and a pipetting suction head, the extraction sleeve and the pipetting suction head can be detachably connected with the bottom of the sleeve, and one end of the piston rod penetrates through the top of the sleeve and extends into an inner cavity of the sleeve; the piston rod is in sliding connection with the sleeve, a first sealing ring is arranged on the side wall of an inner cavity in the top of the sleeve, the piston rod penetrates through the first sealing ring and is tightly attached to the first sealing ring, and a magnetic part is arranged at the end, extending into the inner cavity of the sleeve, of the piston rod. The piston rod can penetrate through the sleeve to move until the magnetic part extends into the bottom of the inner cavity of the extraction sleeve. According to the nucleic acid extraction and pipetting device in the scheme, nucleic acid extraction and pipetting can be completed by using one set of device, the occupation of the surface space of an experiment table during nucleic acid extraction and sample application is reduced, the integration level of experiment equipment can be increased, and the purchase cost of the experiment equipment is reduced.

Description

Technical Field

[0001] The utility model relates to the technical field of nucleic acid extraction and liquid transfer, and in particular to a nucleic acid extraction and liquid transfer integrated device and a nucleic acid extraction and liquid transfer system. Background Art

[0002] At present, when conducting scientific research on nucleic acids, most of them use the magnetic bead method to extract nucleic acids. The main principle of magnetic bead method nucleic acid extraction is: using nanotechnology to improve and modify the surface of superparamagnetic nanoparticles to prepare superparamagnetic silica nanomagnetic beads. The magnetic beads can specifically identify and efficiently bind to nucleic acid molecules at the microscopic interface. Using the superparamagnetism of silica-coated nanomagnetic microspheres, under the action of Chaotropic salt and external magnetic field, DNA and RNA are separated from samples such as blood, animal tissues, food, and pathogenic microorganisms. Magnetic bead method nucleic acid extraction has the advantages of simple operation, short time, safety and non-toxicity, high purity and high concentration of extracted nucleic acids, and can be automated and operated in large quantities.

[0003] After the nucleic acid is extracted and purified, researchers also need to use a specific pipetting device to transfer and spot the extracted and purified nucleic acid in order to carry out subsequent research on the nucleic acid. During the nucleic acid pipetting process, the magnetic bar in the nucleic acid extraction device needs to be removed from the test tube. After that, the nucleic acid extraction device no longer participates in the nucleic acid pipetting process. This requires researchers to use two sets of devices during the nucleic acid extraction and pipetting process, which not only takes up additional limited space on the test bench, but also has the disadvantages of low integration of experimental equipment and increased procurement costs. Utility Model Content

[0004] The utility model provides a nucleic acid extraction and liquid transfer integrated device and a nucleic acid extraction and liquid transfer system to solve the problem that two sets of devices are needed for nucleic acid extraction and liquid transfer in the above-mentioned prior art solutions, which not only occupies the limited space on the test bench, but also makes the integration of experimental equipment low and increases the procurement cost. The device in this solution can also perform nucleic acid liquid transfer after completing nucleic acid extraction. One set of devices can complete nucleic acid extraction and liquid transfer, avoiding excessive occupation of the limited space on the test bench by two sets of devices, improving the integration of experimental equipment, and reducing equipment procurement costs.

[0005] The technical solution adopted by the utility model is: a nucleic acid extraction and liquid transfer integrated device, comprising a sleeve, a piston rod, an extraction sleeve and a liquid transfer pipette tip, the extraction sleeve and the liquid transfer pipette tip can be detachably connected to the bottom of the sleeve, one end of the piston rod passes through the top of the sleeve and extends into the inner cavity of the sleeve, the piston rod is slidably connected to the sleeve, a first sealing ring is provided on the inner cavity side wall of the top of the sleeve, the first sealing ring is fixedly connected to the sleeve, the piston rod passes through the first sealing ring and fits tightly with the first sealing ring, a magnetic part is provided on one end of the piston rod extending into the inner cavity of the sleeve, when the sleeve is connected to the extraction sleeve, the piston rod can pass through the sleeve and move until the magnetic part extends into the bottom of the inner cavity of the extraction sleeve.

[0006] When the magnetic bead method is used to extract nucleic acid, the extraction sleeve is connected to the bottom of the sleeve, and then the piston rod is pushed through the sleeve so that the magnetic part on the piston rod extends into the bottom of the inner cavity of the extraction sleeve, and then the extraction sleeve is extended into the extracting liquid in the test tube, and the sleeve is connected to the driving device. The driving device drives the sleeve to move in the test tube. During the movement of the sleeve, the magnetic beads in the test tube are adsorbed on the outer surface of the extraction sleeve by the magnetic part, and the extraction and purification of the nucleic acid is completed. After the pipette tip is connected to the bottom of the sleeve, the seal between the two is completed. Since a first sealing ring is provided on the sleeve, when the pipette tip is connected to the sleeve, the space between the pipette tip and the first sealing ring is a closed space, and the air pressure in the closed space can be changed by changing the volume of the piston rod in the closed space. When it is necessary to perform pipetting and spotting of nucleic acid, the extraction sleeve on the sleeve is removed from the sleeve, the pipette tip is connected to the sleeve, and then the piston rod is pushed into the sleeve so that the magnetic part is close to the pipette tip, and then the pipette tip is inserted into the test tube containing the purified nucleic acid, and the piston rod is pulled away from the pipette tip. The nucleic acid in the test tube is extracted into the pipette tip. After the pipette tip is aligned with the spotting equipment, the piston rod is pushed toward the pipette tip to remove the nucleic acid liquid inside the pipette tip, and the nucleic acid spotting is completed.

[0007] The nucleic acid extraction and liquid transfer device in this scheme can use one set of devices to complete the extraction and liquid transfer of nucleic acids, reduce the surface space occupied by the laboratory table during nucleic acid extraction and sampling, and can also increase the integration of experimental equipment and reduce the procurement cost of experimental equipment.

[0008] Preferably, the bottom surface of the extraction sleeve is hemispherical. The arc-shaped bottom surface of the extraction sleeve is more closely matched to the shape of the bottom of the test tube, and when the magnetic member extends into the bottom of the inner cavity of the extraction sleeve, the extraction sleeve with an arc-shaped bottom surface has a larger area to adsorb the magnetic beads in the test tube to the extraction sleeve, thereby increasing the number of magnetic beads adsorbed, thereby improving the efficiency of nucleic acid extraction.

[0009] Preferably, the pipette tip is a transparent tip and is provided with a scale on the pipette tip. A second sealing ring is provided on one end of the pipette tip for connecting to the bottom of the sleeve, and the second sealing ring is located in the inner cavity of the pipette tip and is fixedly connected to the pipette tip. When the pipette tip is connected to the sleeve, the second sealing ring fits tightly with the bottom end surface of the sleeve. The pipette tip is a transparent tip and is provided with a scale on the pipette tip. The operator can observe the amount of nucleic acid liquid in the pipette tip, and can control the amount of each spotting when spotting and ensure that the amount of each spotting is roughly the same. After the sealing ring is provided on the pipette tip, the sealing between the pipette tip and the sleeve can be increased to avoid the inability to draw the nucleic acid reagent in the test tube into the pipette tip when the piston rod is extracted due to air leakage between the pipette tip and the sleeve.

[0010] Preferably, a handle is provided on the end of the piston rod away from the magnetic member, and the handle is located outside the sleeve and fixedly connected to the piston rod. After the handle is provided, the operator can pull the piston rod by the handle, which is more convenient for operation. In addition, the handle can be connected to an automated instrument, and the length of the piston rod extending into the sleeve can be controlled by the automated instrument, thereby completing automated liquid transfer sampling.

[0011] Preferably, the handle is provided with a mounting seat for fixing the extraction sleeve or the pipette tip, the mounting seat is fixedly connected to the handle, and the extraction sleeve and the pipette tip can be detachably connected to the mounting seat. It also includes a dust cover for covering the mounting seat, the dust cover is threadedly connected to the mounting seat, and the extraction sleeve or the pipette tip can be located in the inner cavity of the dust cover. After the mounting seat is set, when the extraction sleeve or the pipette tip is not in use, it can be fixed to the mounting seat to avoid loss of experimental equipment. The dust cover can cover the extraction sleeve or the pipette tip on the mounting seat to prevent it from being contaminated by impurities such as dust in the air.

[0012] Preferably, a cavity is provided inside the piston rod, and a reinforcing rib is provided inside the cavity. Providing a cavity inside the piston rod can further reduce the weight of the piston rod, and avoid the piston rod being too heavy, which affects the operator's hand feeling when pulling the piston rod. At the same time, reinforcing ribs are provided in the cavity, and the reinforcing ribs can prevent the piston rod from reducing its strength due to the cavity.

[0013] Preferably, a driving gear for connecting with an external driving mechanism is sleeved on the outer wall of the sleeve, and the driving gear is fixedly connected to the sleeve. The driving gear is sleeved on the outer wall of the sleeve, so that the driving mechanism can drive the driving gear to rotate through a power gear meshing with the driving gear, and then the driving sleeve drives the extraction sleeve to rotate in the test tube to complete the nucleic acid extraction.

[0014] A nucleic acid extraction and liquid transfer system comprises a driving unit, a base and a nucleic acid extraction and liquid transfer integrated device. The nucleic acid extraction and liquid transfer integrated device is the above-mentioned nucleic acid extraction and liquid transfer integrated device. The base comprises a fixed frame for placing a test tube and a support frame for placing the nucleic acid extraction and liquid transfer integrated device. There are several nucleic acid extraction and liquid transfer integrated devices, and the several nucleic acid extraction and liquid transfer integrated devices are arranged in an array on the support frame. Each nucleic acid extraction and liquid transfer integrated device has a test tube corresponding to it on the fixed frame. The driving gears in two adjacent nucleic acid extraction and liquid transfer integrated devices are meshed with each other. The driving unit comprises a power gear, and the power gear is meshed with one of the several driving gears. When nucleic acid extraction is performed, the driving unit drives the power gear to rotate, and the rotation of the power gear drives the driving gear meshed with it to rotate, and then drives the sleeve where the driving gear is located to rotate, and nucleic acid extraction is performed. The rotation of a driving gear can drive the adjacent driving gear meshed with it to rotate. By analogy, the rotation of a driving gear can drive all the driving gears on the support frame to rotate, that is, it can drive all the sleeves on the driving frame to rotate, and nucleic acid extraction is performed.

[0015] Compared with the prior art, the beneficial effect of the utility model is that the nucleic acid extraction and liquid transfer device in the present scheme can use one set of devices to complete the extraction and liquid transfer of nucleic acids, reduce the occupation of the surface space of the laboratory table during nucleic acid extraction and sampling, and can also increase the integration of experimental equipment and reduce the procurement cost of experimental equipment. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is a schematic structural diagram of Embodiment 1 of a nucleic acid extraction and liquid transfer integrated device of the utility model;

[0017] Figure 2 This is a schematic diagram of the internal structure of a sleeve and a piston rod of Example 1 of a nucleic acid extraction and liquid transfer integrated device of the utility model;

[0018] Figure 3 This is a schematic diagram of the working state of nucleic acid extraction of Example 1 of a nucleic acid extraction and liquid transfer integrated device of the utility model;

[0019] Figure 4 This is a schematic diagram of the working state of liquid transfer in Example 1 of a nucleic acid extraction and liquid transfer integrated device of the utility model;

[0020] Figure 5 This is a schematic diagram of the nucleic acid extraction working state of Example 2 of a nucleic acid extraction and liquid transfer integrated device of the utility model;

[0021] Figure 6 This is a schematic diagram of the working state of liquid transfer in Example 2 of a nucleic acid extraction and liquid transfer integrated device of the utility model;

[0022] Figure 7It is a schematic diagram of the partial internal structure of the piston rod and sleeve of Example 2 of a nucleic acid extraction and liquid transfer integrated device of the utility model;

[0023] Figure 8 It is a schematic structural diagram of a liquid transfer tip of a nucleic acid extraction and liquid transfer integrated device according to embodiment 2 of the utility model;

[0024] Fig. 9 The utility model is a structural schematic diagram of a nucleic acid extraction and liquid transfer system. DETAILED DESCRIPTION

[0025] The drawings are only for illustrative purposes and cannot be construed as limiting the present invention. To better illustrate the present embodiment, some parts in the drawings may be omitted, enlarged, or reduced, and do not represent the size of the actual product. For those skilled in the art, it is understandable that some well-known structures and their descriptions in the drawings may be omitted. The positional relationships described in the drawings are only for illustrative purposes and cannot be construed as limiting the present invention.

[0026] The same or similar numbers in the drawings of the embodiments of the present invention correspond to the same or similar parts; in the description of the present invention, it should be understood that if the terms "upper", "lower", "left", "right", "long", "short" and the like indicate directions or positional relationships based on the directions or positional relationships shown in the drawings, they are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific direction, be constructed and operated in a specific direction. Therefore, the terms describing the positional relationship in the drawings are only used for illustrative purposes and cannot be understood as limitations on this patent. For ordinary technicians in this field, the specific meanings of the above terms can be understood according to specific circumstances.

[0027] The technical solution of the utility model is further described in detail below through specific embodiments and in conjunction with the accompanying drawings:

[0028] Example 1

[0029] like Figure 1-Figure 4 The figure shows an embodiment 1 of an integrated nucleic acid extraction and liquid transfer device, comprising a sleeve 1, a piston rod 2, an extraction sleeve 3 and a pipette tip 4. The extraction sleeve 3 and the pipette tip 4 can be threadedly connected to the bottom of the sleeve 1. One end of the piston rod 2 passes through the top of the sleeve 1 and extends into the inner cavity of the sleeve 1. The piston rod 2 is slidably connected to the sleeve 1. A first sealing ring 5 is provided on the side wall of the inner cavity at the top of the sleeve 1. The first sealing ring 5 is fixedly connected to the sleeve 1. The piston rod 2 passes through the first sealing ring 5 and fits tightly with the first sealing ring 5. A magnetic part 6 is provided on the end of the piston rod 2 that extends into the inner cavity of the sleeve 1. When the sleeve 1 is connected to the extraction sleeve 3, the piston rod 2 can pass through the sleeve 1 and move to the magnetic part 6 to extend into the bottom of the inner cavity of the extraction sleeve 3.

[0030] The working principle or working process of this embodiment is as follows: when the magnetic bead method is used to extract nucleic acid, the extraction sleeve 3 is connected to the bottom of the sleeve 1, and then the piston rod 2 is pushed through the sleeve 1, so that the magnetic member 6 on the piston rod 2 extends into the bottom of the inner cavity of the extraction sleeve 3, and then the extraction sleeve 3 is extended into the extracting liquid in the test tube 14, and the sleeve 1 is connected to the driving device, and the driving device drives the sleeve 1 to move in the test tube 14. During the movement of the sleeve 1, the magnetic beads in the test tube 14 are adsorbed on the outer surface of the extraction sleeve 3 by the magnetic member 6, and the extraction and purification of nucleic acid is completed. After the pipette tip 4 is connected to the bottom of the sleeve 1, the seal between the two is completed. Since the first sealing ring 5 is provided on the sleeve 1, when the pipette tip 4 is connected to the sleeve 1, the space between the pipette tip 4 and the first sealing ring 5 is a closed space, and the air pressure in the closed space can be changed by changing the volume of the piston rod 2 in the closed space. When it is necessary to perform pipetting and spotting of nucleic acid, the extraction sleeve 3 on the sleeve 1 is removed from the sleeve 1, the pipette tip 4 is connected to the sleeve 1, and then the piston rod 2 is pushed into the sleeve 1, so that the magnetic member 6 is close to the pipette tip 4, and then the pipette tip 4 is inserted into the test tube 14 containing the purified nucleic acid, and the piston rod 2 is pulled away from the pipette tip 4. The nucleic acid in the test tube 14 is extracted into the pipette tip 4. After the pipette tip 4 is aligned with the spotting equipment, the piston rod 2 is pushed to move toward the pipette tip 4 to remove the nucleic acid liquid inside the pipette tip 4, and the nucleic acid spotting is completed.

[0031] Beneficial effects of this embodiment: The nucleic acid extraction and pipetting device in this scheme can use one set of devices to complete nucleic acid extraction and pipetting, reduce the surface space occupied by the laboratory table during nucleic acid extraction and sampling, and can also increase the integration of experimental equipment and reduce the procurement cost of experimental equipment.

[0032] Example 2

[0033] Embodiment 2 of a nucleic acid extraction and liquid transfer integrated device, as Figure 5-Figure 8 As shown, based on Example 1, the structure of the device is further limited.

[0034] Specifically, the bottom surface of the extraction sleeve 3 is hemispherical. The pipette tip 4 is a transparent tip and is provided with a scale. The end of the pipette tip 4 for connecting with the bottom of the sleeve 1 is provided with a second sealing ring 7, which is located in the inner cavity of the pipette tip 4 and is fixedly connected to the pipette tip 4. When the pipette tip 4 is connected to the sleeve 1, the second sealing ring 7 is tightly fitted with the bottom end surface of the sleeve 1.

[0035] Specifically, a handle 8 is provided on the end of the piston rod 2 away from the magnetic member 6. The handle 8 is located outside the sleeve 1 and fixedly connected to the piston rod 2. A mounting seat 9 for fixing the extraction sleeve 3 or the pipette tip 4 is provided on the handle 8. The mounting seat 9 is fixedly connected to the handle 8. The extraction sleeve 3 and the pipette tip 4 can be threadedly connected to the mounting seat 9. A dust cover 10 for covering the mounting seat 9 is also included. The dust cover 10 is threadedly connected to the mounting seat 9. The extraction sleeve 3 or the pipette tip 4 can be located in the inner cavity of the dust cover 10. A cavity is provided inside the piston rod 2, and a reinforcing rib is provided inside the cavity.

[0036] Specifically, a driving gear 11 for connecting with an external driving mechanism is sleeved on the outer wall of the sleeve 1 , and the driving gear 11 is fixedly connected to the sleeve 1 .

[0037] The beneficial effects of this embodiment are as follows: the arcuate surface at the bottom of the extraction sleeve 3 is more closely matched to the shape of the bottom of the test tube 14, and when the magnetic member 6 extends into the bottom of the inner cavity of the extraction sleeve 3, the extraction sleeve 3 with an arcuate bottom has a larger area to adsorb the magnetic beads in the test tube 14 to the extraction sleeve 3, thereby increasing the number of magnetic beads absorbed, and thus improving the efficiency of nucleic acid extraction. The pipette tip 4 is a transparent tip and a scale is provided on the pipette tip 4. The operator can observe the amount of nucleic acid liquid in the pipette tip 4, and can control the amount of each spotting when spotting and ensure that the amount of each spotting is roughly the same. After the second sealing ring 7 is provided on the pipette tip 4, the sealing between the pipette tip 4 and the sleeve 1 can be increased to avoid the inability to draw the nucleic acid reagent in the test tube 14 into the pipette tip 4 when the piston rod 2 is extracted due to air leakage between the pipette tip 4 and the sleeve 1. After the handle 8 is set, the operator can pull the piston rod 2 through the handle 8, which is more convenient for operation. In addition, the handle can also be connected to the automated instrument, and the length of the piston rod extending into the sleeve can be controlled by the automated instrument to complete the automated liquid transfer. After the mounting seat 9 is set, when the extraction sleeve 3 or the pipetting tip 4 is not in use, it can be fixed on the mounting seat 9 to avoid the loss of experimental equipment. The dust cover 10 can cover the extraction sleeve 3 or the pipetting tip 4 on the mounting seat 9 to avoid it being contaminated by impurities such as dust in the air. A cavity is provided in the piston rod 2, which can further reduce the weight of the piston rod 2 and avoid the excessive weight of the piston rod 2, which affects the feel of the operator when pulling the piston rod 2. At the same time, a reinforcing rib is provided in the cavity, and the reinforcing rib can prevent the piston rod 2 from being reduced in strength after the cavity is set. A driving gear 11 is sleeved on the outer wall of the sleeve 1, so that the driving mechanism can drive the driving gear 11 to rotate through the power gear meshing with the driving gear 11, and then drive the sleeve 1 to drive the extraction sleeve 3 to rotate in the test tube 14 to complete the nucleic acid extraction.

[0038] Example 3

[0039] A nucleic acid extraction pipetting system, such as Fig. 9As shown, it includes a driving unit, a base and a nucleic acid extraction and liquid transfer integrated device. The nucleic acid extraction and liquid transfer integrated device is a nucleic acid extraction and liquid transfer integrated device described in Example 2. The base includes a fixing frame 12 for placing a test tube 14 and a supporting frame 13 for placing the nucleic acid extraction and liquid transfer integrated device. There are several nucleic acid extraction and liquid transfer integrated devices, and the several nucleic acid extraction and liquid transfer integrated devices are arranged in an array on the supporting frame 13. Each nucleic acid extraction and liquid transfer integrated device has a test tube 14 corresponding to it on the fixing frame 12. The driving gears 11 in two adjacent nucleic acid extraction and liquid transfer integrated devices are meshed with each other. The driving unit includes a power gear, and the power gear is meshed with one of the several driving gears 11.

[0040] The working principle or working process of this embodiment is as follows: when nucleic acid extraction is performed, the driving unit drives the power gear to rotate, and the rotation of the power gear drives the driving gear 11 meshed with it to rotate, and then drives the sleeve 1 where the driving gear 11 is located to rotate, and nucleic acid extraction is performed. The rotation of one driving gear 11 can drive the adjacent driving gear 11 meshed with it to rotate, and so on, the rotation of one driving gear 11 can drive all the driving gears 11 on the support frame 13 to rotate, that is, it can drive all the sleeves 1 on the driving frame to rotate, and nucleic acid extraction is performed.

[0041] Beneficial effects of this embodiment: The nucleic acid extraction and liquid transfer system in this scheme can simultaneously drive multiple groups of nucleic acid extraction devices to perform nucleic acid extraction through a driving unit, thereby improving the efficiency of nucleic acid extraction. Obviously, the above embodiments of the utility model are only examples for clearly illustrating the utility model, and are not limitations on the implementation methods of the utility model. For ordinary technicians in the relevant field, other different forms of changes or modifications can be made on the basis of the above description. It is not necessary and impossible to list all the implementation methods here. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the utility model should be included in the scope of protection of the claims of the utility model.

Claims

1. A nucleic acid extraction and liquid transfer integrated device, characterized in that: The invention comprises a sleeve (1), a piston rod (2), an extraction sleeve (3) and a liquid transfer tip (4), wherein the extraction sleeve (3) and the liquid transfer tip (4) can be detachably connected to the bottom of the sleeve (1). One end of the piston rod (2) passes through the top of the sleeve (1) and extends into the inner cavity of the sleeve (1); the piston rod (2) is slidably connected to the sleeve (1); a first sealing ring (5) is provided on the inner cavity side wall of the top of the sleeve (1); the first sealing ring (5) is fixedly connected to the sleeve (1); the piston rod (2) passes through the first sealing ring (5) and fits tightly with the first sealing ring (5); A magnetic member (6) is provided on one end of the piston rod (2) that extends into the inner cavity of the sleeve (1); when the sleeve (1) is connected to the extraction sleeve (3), the piston rod (2) can pass through the sleeve (1) and move to the bottom of the inner cavity of the extraction sleeve (3) where the magnetic member (6) extends.

2. A nucleic acid extraction and liquid transfer integrated device according to claim 1, characterized in that: The bottom of the extraction sleeve (3) is hemispherical.

3. A nucleic acid extraction and liquid transfer integrated device according to claim 1, characterized in that: The pipette tip (4) is a transparent tip and a scale is provided on the pipette tip (4).

4. A nucleic acid extraction and liquid transfer integrated device according to claim 3, characterized in that: A second sealing ring (7) is provided at one end of the pipette tip (4) for connecting with the bottom of the sleeve (1); the second sealing ring (7) is located in the inner cavity of the pipette tip (4) and is fixedly connected to the pipette tip (4); when the pipette tip (4) is connected to the sleeve (1), the sealing ring is tightly fitted with the bottom end surface of the sleeve (1).

5. The nucleic acid extraction and liquid transfer integrated device according to claim 1, characterized in that: A handle (8) is provided on one end of the piston rod (2) away from the magnetic member (6); the handle (8) is located outside the sleeve (1) and is fixedly connected to the piston rod (2).

6. The nucleic acid extraction and liquid transfer integrated device according to claim 5, characterized in that: The handle (8) is provided with a mounting seat (9) for fixing the extraction sleeve (3) or the pipette tip (4); the mounting seat (9) is fixedly connected to the handle (8); and both the extraction sleeve (3) and the pipette tip (4) can be detachably connected to the mounting seat (9).

7. The nucleic acid extraction and liquid transfer integrated device according to claim 6, characterized in that: It also includes a dust cover (10) for covering the mounting seat (9), wherein the dust cover (10) is threadedly connected to the mounting seat (9), and the extraction sleeve (3) or the pipette tip (4) can be located in the inner cavity of the dust cover (10).

8. The nucleic acid extraction and liquid transfer integrated device according to claim 5, characterized in that: A cavity is provided inside the piston rod (2), and reinforcing ribs are provided inside the cavity.

9. A nucleic acid extraction and liquid transfer integrated device according to any one of claims 1 to 8, characterized in that: A driving gear (11) for connecting to an external driving mechanism is sleeved on the outer wall of the sleeve (1), and the driving gear (11) is fixedly connected to the sleeve (1).

10. A nucleic acid extraction and liquid transfer system, characterized in that: The invention comprises a driving part, a base and a nucleic acid extraction and liquid transfer integrated device, wherein the nucleic acid extraction and liquid transfer integrated device is a nucleic acid extraction and liquid transfer integrated device as claimed in claim 9, The base comprises a fixing frame (12) for placing a test tube (14) and a supporting frame (13) for placing the nucleic acid extraction and liquid transfer integrated device. There are a plurality of nucleic acid extraction and liquid transfer integrated devices, and the plurality of nucleic acid extraction and liquid transfer integrated devices are arranged in an array on the supporting frame (13). Each nucleic acid extraction and liquid transfer integrated device has a test tube (14) corresponding to it on the fixing frame (12). The driving gears (11) in two adjacent nucleic acid extraction and liquid transfer integrated devices mesh with each other, and the driving part comprises a power gear, which meshes with one of the plurality of driving gears (11).

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