Full-automatic thin-layer chromatography spray box

Abstract

The utility model provides a full-automatic thin-layer chromatography spraying box. The full-automatic thin-layer chromatography spraying box comprises a box body, a spraying mechanism and an automatic liquid taking mechanism, the spraying mechanism and the automatic liquid taking mechanism are arranged in a first containing cavity and a second containing cavity in the lower portion of the box body respectively. The automatic liquid taking mechanism is connected with the spraying mechanism through a pipeline so as to suck the color developing agent in the second accommodating cavity to spray the thin-layer plate in the first accommodating cavity. According to the utility model, the spraying mechanism and the automatic liquid taking mechanism are arranged in the box body and are connected and controlled by the controller on the box body, and the spraying and color developing processes can be automatically completed through the controller, so that the automation and intelligence of the spraying process are realized, and the spraying uniformity and efficiency are improved.

Description

Technical Field

[0001] The utility model relates to the technical field of thin layer chromatography, in particular to a full-automatic thin layer chromatography spray box. Background Art

[0002] Thin layer chromatography is a commonly used color development and analysis technique, widely used in the fields of chemistry, biology, medicine, etc. Its basic principle is to apply a suitable stationary phase on a glass plate, plastic or aluminum substrate to form a uniform thin layer. After spotting and developing, the relative shift value (Rf) is compared with the relative shift value (Rf) of the chromatogram obtained by the same method with a suitable reference substance, which is used for drug identification, impurity inspection or content determination. After the compounds are spotted and developed during the thin layer chromatography process, a color development step is required before the chromatography results can be observed and analyzed. Different compounds have different development effects under different developing agent conditions, and the optional color development methods are also different.

[0003] However, the existing TLC operation usually uses the developer spray method, which requires manual spraying, which is not only inefficient, but also difficult to control the spraying time and flow rate. Therefore, how to realize the automation of TLC operation and improve efficiency and accuracy is a problem that needs to be solved urgently. Utility Model Content

[0004] The technical problem to be solved by the utility model is: in view of the defects of the prior art, a full-automatic thin layer chromatography spray box is provided which can automatically complete the spraying and coloring process and improve the operation stability and efficiency.

[0005] The utility model adopts the following technical solutions to solve the above technical problems:

[0006] A fully automatic thin layer chromatography spray box, comprising a box body, a spray mechanism and an automatic liquid taking mechanism, wherein:

[0007] The spray mechanism and the automatic liquid taking mechanism are respectively arranged in the first accommodating chamber and the second accommodating chamber at the lower part of the box body;

[0008] The automatic liquid taking mechanism is connected to the spray mechanism through a pipeline to absorb the developer in the second accommodating chamber and spray the thin layer plate in the first accommodating chamber.

[0009] Preferably, the lower part of the box body is provided with the first accommodating cavity and the second accommodating cavity arranged side by side, wherein:

[0010] The connecting window between the first accommodating cavity and the second accommodating cavity and the observation windows on both sides are respectively provided with first observation glasses, and the openings at the rear ends are respectively provided with second observation glasses.

[0011] Preferably, the spray mechanism comprises a spray head and a gas solenoid valve, wherein:

[0012] The top end of the spray head is detachably mounted in the screw hole at the top of the first accommodating chamber, and the liquid inlet thereof is connected to the automatic liquid taking mechanism via a pipeline through the gas solenoid valve.

[0013] Preferably, the automatic liquid taking mechanism comprises a first longitudinal screw slide, a second longitudinal screw slide, a transverse screw slide, a lifting and lowering liquid suction mechanism and an automatic liquid taking device, wherein:

[0014] The two ends of the transverse screw slide are respectively arranged on the slide seats of the first longitudinal screw slide and the second longitudinal screw slide, and the lifting and lowering liquid suction mechanism and the automatic liquid extractor are installed on the lifting and lowering slider thereon.

[0015] Preferably, the lifting and liquid suction mechanism comprises a lifting and liquid suction mounting platform, an L-shaped guide plate, a transmission gear and a drive motor, wherein:

[0016] The lifting installation platform is arranged on the lifting slider of the transverse screw slide, and the L-shaped guide plate and the transmission gear are vertically installed on the outer side wall of the lifting installation platform, and the transmission gear is connected to the output shaft of the driving motor.

[0017] Preferably, the automatic liquid dispenser comprises a liquid dispenser body and a C-shaped guide strip, wherein:

[0018] The upper and lower ends of the C-shaped guide bar are fixedly connected to the liquid extractor body, and a guide groove for accommodating the L-shaped guide plate is formed between the inner side of the C-shaped guide bar and the liquid extractor body, and a bar rack matched with a transmission gear is provided on the outer side wall.

[0019] Preferably, the fully automatic thin layer chromatography spray box further comprises a thin layer plate drawer and a developer drawer, wherein:

[0020] The thin layer plate drawer and the developer drawer are respectively drawable and arranged in the first accommodating cavity and the second accommodating cavity.

[0021] Preferably, first observation windows are respectively provided on the left and right side walls of the thin-layer board drawer, a second observation window is provided on the upper portion of the rear plate, and a plurality of thin-layer board support columns are arranged inside.

[0022] More preferably, a third observation window is respectively provided on the left and right side walls of the developer drawer, a fourth observation window is provided on the upper portion of the rear plate, and a reagent support frame is provided inside.

[0023] Preferably, the fully automatic thin layer chromatography spray box further comprises a controller disposed at the upper front end of the box body, wherein:

[0024] The controller is electrically connected to the spray mechanism and the automatic liquid taking mechanism respectively to control the spray mechanism and the automatic liquid taking mechanism to work according to instructions.

[0025] The utility model adopts the above technical solution, and compared with the prior art, has the following technical effects:

[0026] The fully automatic thin layer chromatography spray box provided by the utility model has a spray mechanism and an automatic liquid taking mechanism arranged in the box body, and is connected and controlled by a controller on the box body, so that the automation and intelligence of the spray process are realized through the controller, and the uniformity and efficiency of the spray are improved; at the same time, the box body adopts a fully closed management, which can maintain constant environmental conditions and prevent pollutants from leaking out, which is conducive to improving the operation stability; and transparent observation glass is adopted on the two side walls and the rear wall of the box body, so that the operator can observe the color development analysis process in real time, adjust the required color developer position and spray time in time, and ensure the accuracy of the operation. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 The three-dimensional structure diagram of a fully automatic thin layer chromatography spray box of the utility model Figure 1 ;

[0028] Figure 2 The three-dimensional structure diagram of a fully automatic thin layer chromatography spray box of the utility model Figure 2 ;

[0029] Figure 3 This is a schematic cross-sectional view of a fully automatic thin layer chromatography spray box of the utility model;

[0030] Figure 4 This is a schematic diagram of the assembly structure of a fully automatic thin layer chromatography spray box of the utility model. Figure 1 ;

[0031] Figure 5 This is a schematic diagram of the assembly structure of a fully automatic thin layer chromatography spray box of the utility model. Figure 2 ;

[0032] Figure 6 This is a schematic diagram of the structure of the box in a fully automatic thin layer chromatography spray box of the utility model. Figure 1 ;

[0033] Figure 7 This is a schematic diagram of the structure of the box in a fully automatic thin layer chromatography spray box of the utility model. Figure 2 ;

[0034] Figure 8 This is a schematic diagram of the structure of an automatic liquid extraction mechanism in a fully automatic thin layer chromatography spray box of the utility model. Figure 1 ;

[0035] Fig. 9 This is a schematic diagram of the structure of an automatic liquid extraction mechanism in a fully automatic thin layer chromatography spray box of the utility model. Figure 2 ;

[0036] Fig.10 This is a schematic diagram of the structure of an automatic liquid extraction mechanism in a fully automatic thin layer chromatography spray box of the utility model. Figure 3 ;

[0037] Fig.11 This is a schematic diagram of the assembly structure of an automatic liquid taking mechanism in a fully automatic thin layer chromatography spray box of the utility model;

[0038] Fig.12 This is a schematic diagram of the assembly structure of a lifting and lowering liquid suction mechanism and an automatic liquid extractor in a fully automatic thin layer chromatography spray box of the utility model;

[0039] Fig.13 This is a structural schematic diagram of a lifting and liquid aspiration mechanism in a fully automatic thin layer chromatography spray box of the utility model;

[0040] Fig.14 The utility model is a structural schematic diagram of a medium-liter automatic liquid dispenser for a fully automatic thin-layer chromatography spray box. DETAILED DESCRIPTION

[0041] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments.

[0042] Based on the embodiments of the present utility model, all other embodiments obtained by ordinary technicians in the field without making any creative work shall fall within the scope of protection of the present utility model.

[0043] In some embodiments, Figure 1 , Figure 2 , Figure 3 , Figure 4 and Figure 5 As shown, a fully automatic thin layer chromatography spray box is provided, comprising a box body 100, a thin layer plate drawer 200 and a developer drawer 300, a spray mechanism 400 and an automatic liquid dispensing mechanism 500. The thin layer plate drawer 200 and the developer drawer 300 are respectively drawable and arranged in the first accommodating chamber 101 and the second accommodating chamber 102. The spray mechanism 400 and the automatic liquid dispensing mechanism 500 are respectively arranged in the first accommodating chamber 101 and the second accommodating chamber 102 at the lower part of the box body 100;

[0044] The spray mechanism 400 is located above the thin layer plate in the developer drawer 300 and is used to spray the developer onto the thin layer plate. The automatic liquid taking mechanism 500 is located above the developer drawer 300 and can be moved in the second accommodating cavity 102 to absorb the corresponding developer in the developer drawer 300.

[0045] The automatic liquid taking mechanism 500 is connected to the spray mechanism 400 through a pipeline, and the spray mechanism 400 and the automatic liquid taking mechanism 500 are both controlled by the controller 600. When in use, the color developer in the second accommodating chamber 102 is sucked by the automatic liquid taking mechanism 500, and the sucked color developer is sprayed on the thin layer plate in the first accommodating chamber 101 by the spray mechanism 400, and the controller 600 can accurately control the position of the color developer to be sucked and the spraying time.

[0046] In some of the embodiments, Figure 1 , Figure 2 , Figure 6 and Figure 7 As shown, the box body 100 adopts an integrated structure, and the first accommodating cavity 101 and the second accommodating cavity 102 arranged side by side are opened at the lower part of the box body 100. If necessary, a third accommodating cavity can be provided at the upper part of the box body 100 for arranging a storage drawer.

[0047] In order to facilitate real-time observation of the operating status of the equipment in the two accommodating chambers at the bottom, first observation glasses 103 are respectively provided at the connection window between the first accommodating chamber 101 and the second accommodating chamber 102 and the observation windows on both sides, and second observation glasses 104 are respectively provided at the openings at the rear end. The first observation glass 103 and the second observation glass 104 are made of tempered glass, which is both observable and has a certain tempering strength.

[0048] In order to maintain constant environmental conditions in the box and prevent pollutants from leaking out, the first observation glass 103 and the second observation glass 104 are both sealed and installed on the box 100 using screws and sealing strips, which is conducive to improving operational stability. Of course, the first observation glass 103 and the second observation glass 104 can be magnetically attracted glass and fixedly installed on the box 100 using an embedded structure as needed, which is convenient for maintenance and adjustment.

[0049] In some embodiments, the spray mechanism 400 is composed of a spray head 401 and a gas solenoid valve 402. The top of the spray head 401 is detachably mounted in a screw hole at the top of the first accommodating chamber 101, and its liquid inlet is connected to the automatic liquid taking mechanism 500 through the gas solenoid valve 402 via a pipeline.

[0050] The spray mechanism 400 also includes an air compressor disposed on the upper portion of the housing 100, which provides high-pressure gas to the spray head 401 for spraying the thin layer plate through the spray head 401. The spray head 401 sprays the coloring liquid through the siphon effect and sprays it evenly on the thin layer plate.

[0051] In addition, the spray mechanism 400 may include one or more spray heads 401, and the number of the spray heads 401 may be set as required. The spray head 401 may be fixed on the box 100 or movably arranged in the box 100. The controller 600 controls the operation of the spray head 401 according to the calculated spray amount.

[0052] According to actual application requirements, the spray head 401 can be a micro nozzle or a conventional nozzle, which can be selected as needed. In the implementation process, the spray mechanism 400 sprays the thin layer plate uniformly to ensure accuracy and repeatability.

[0053] In some of the embodiments, Figure 8 , Fig. 9 , Fig.10 , Fig.11 and Fig.12 As shown, the automatic liquid taking mechanism 500 mainly includes a first longitudinal screw slide 510, a second longitudinal screw slide 520, a transverse screw slide 530, a lifting liquid suction mechanism 540 and an automatic liquid taker 550. The first longitudinal screw slide 510, the second longitudinal screw slide 520, the transverse screw slide 530, and the lifting liquid suction mechanism 540 constitute an XYZ axis for controlling the color developer sampling operation.

[0054] Among them, the two ends of the transverse screw slide 530 are respectively arranged on the sliding seats of the first longitudinal screw slide 510 and the second longitudinal screw slide 520, that is, the transverse screw slide 530 can move forward and backward through the first longitudinal screw slide 510 and the second longitudinal screw slide 520.

[0055] The lifting and lowering liquid suction mechanism 540 and the automatic liquid dispenser 550 are installed on the lifting and lowering slider 531 of the transverse screw slide 530. The lifting and lowering liquid suction mechanism 540 can drive the automatic liquid dispenser 550 to move up and down to insert the needle into the corresponding color developing reagent bottle.

[0056] Specifically, Fig.12 and Fig.13 As shown, the lifting and liquid suction mechanism 540 includes a lifting installation platform 541, an L-shaped guide plate 542, a transmission gear 543 and a driving motor 544. The lifting and liquid suction mechanism 540 is arranged on the lifting slider 531 of the transverse screw slide 530, and the lifting and liquid suction mechanism 540 can slide left and right as a whole along with the lifting slider 531.

[0057] The L-shaped guide plate 542 and the transmission gear 543 are vertically installed on the outer side wall of the lifting installation platform 541, and the transmission gear 543 is connected to the output shaft of the driving motor 544. The L-shaped guide plate 542 plays a guiding and limiting role, and a C-shaped guide bar 552 is movably arranged on it. The C-shaped guide bar 552 is meshed and connected with the transmission gear 543. When the driving motor 544 starts the transmission gear 543 to rotate, the C-shaped guide bar 552 can be synchronously driven to move up and down along the L-shaped guide plate 542.

[0058] like Fig.12 and Fig.14 As shown, the automatic liquid dispenser 550 includes a liquid dispenser body 551 and a C-shaped guide bar 552. The upper and lower ends of the C-shaped guide bar 552 are fixedly connected to the liquid dispenser body 551, and a guide groove 553 for accommodating the L-shaped guide plate 542 is formed between the inner side of the C-shaped guide bar 552 and the liquid dispenser body 551, and a bar-shaped rack 554 matching the transmission gear 543 is provided on the outer wall.

[0059] In some of the embodiments, Figure 1 , Figure 2 , Figure 4 and Figure 5 As shown, first observation windows 201 are respectively provided on the left and right side walls of the thin layer board drawer 200, a second observation window 202 is provided on the upper part of the rear plate, and a plurality of thin layer board support columns 203 are provided inside. The first observation window 201 and the second observation window 202 are square grooves provided on the drawer plate body, and the thin layer board support columns 203 are used to place the thin layer boards.

[0060] Correspondingly, a third observation window 301 is respectively provided on the left and right side walls of the developer drawer 300, a fourth observation window 302 is provided on the upper part of the rear plate, and a reagent support frame 303 is provided inside. The reagent support frame 303 adopts a modular liquid sample tray structure, on which placement holes of different calibers are provided for placing liquid bottles of different calibers.

[0061] Furthermore, in some of the embodiments, Figure 1 , Figure 4 , Figure 5 , Figure 6 and Figure 7 As shown, the fully automatic thin layer chromatography spray box further includes a controller 600, which is disposed at the upper front end of the box body 100. The controller 600 is provided with a control panel that can display the spray flow rate and time. The controller 600 is electrically connected to the spray mechanism 400 and the automatic liquid taking mechanism 500, respectively, to control the spray mechanism 400 and the automatic liquid taking mechanism 500 to work according to instructions.

[0062] The controller 600 adopts a PLC control panel, which can be a microprocessor or a microcontroller, for receiving instructions input by the user and controlling the operation of the spray mechanism 400 and the automatic liquid taking mechanism 500 according to the instructions. Specifically, the controller 600 can automatically perform corresponding operations according to the required developer position and spray time input by the user.

[0063] The controller 600 can receive sample information and color development conditions input by the user through an operation interface. The operation interface can be a touch screen or a keyboard input device, and the user can input information such as sample name, concentration, color development conditions, etc. through the operation interface.

[0064] After receiving the instruction input by the user, the controller 600 automatically calculates the spray volume and the color development time according to the instruction. The controller 600 can also be built-in with a preset algorithm or calculate according to the sample information and color development conditions input by the user. The calculated spray volume and color development time can be a range value or a specific value. The controller 600 controls the spray mechanism 400 and the automatic liquid taking mechanism 500 to perform corresponding operations according to the calculation results.

[0065] Finally, a few points should be explained: First, in the description of this application, it should be noted that, unless otherwise specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, which may refer to mechanical connection or electrical connection, or internal communication between two components, or direct connection. "upper", "lower", "left", "right", etc. are only used to indicate relative positional relationships. When the absolute position of the object being described changes, the relative positional relationship may change;

[0066] Secondly, the drawings of the embodiments disclosed in the present utility model only involve structures related to the embodiments disclosed in the present utility model, and other structures can refer to the general design. In the absence of conflict, the same embodiment and different embodiments of the present utility model can be combined with each other;

[0067] Finally, the above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A fully automatic thin layer chromatography spray box, characterized in that, It includes a box body, a spray mechanism and an automatic liquid dispensing mechanism, wherein: The spray mechanism and the automatic liquid taking mechanism are respectively arranged in the first accommodating chamber and the second accommodating chamber at the lower part of the box body; The automatic liquid taking mechanism is connected to the spray mechanism through a pipeline to absorb the developer in the second accommodating chamber and spray the thin layer plate in the first accommodating chamber; The lower part of the box body is provided with the first accommodating cavity and the second accommodating cavity arranged side by side, wherein: The connecting window between the first accommodating cavity and the second accommodating cavity and the observation windows on both sides are respectively provided with first observation glasses, and the openings at the rear end are respectively provided with second observation glasses; The spray mechanism comprises a spray head and a gas solenoid valve, wherein: The top end of the spray head is detachably mounted in the screw hole at the top of the first accommodating chamber, and its liquid inlet is connected to the automatic liquid taking mechanism through the gas solenoid valve via a pipeline; The automatic liquid taking mechanism comprises a first longitudinal screw slide, a second longitudinal screw slide, a transverse screw slide, a lifting and lowering liquid suction mechanism and an automatic liquid taking device, wherein: The two ends of the transverse screw slide are respectively arranged on the slide seats of the first longitudinal screw slide and the second longitudinal screw slide, and the lifting and lowering liquid suction mechanism and the automatic liquid extractor are installed on the lifting and lowering slider thereon.

2. The fully automatic thin layer chromatography spray box according to claim 1, characterized in that: The lifting and liquid suction mechanism comprises a lifting and mounting platform, an L-shaped guide plate, a transmission gear and a driving motor, wherein: The lifting installation platform is arranged on the lifting slider of the transverse screw slide, and the L-shaped guide plate and the transmission gear are vertically installed on the outer side wall of the lifting installation platform, and the transmission gear is connected to the output shaft of the driving motor.

3. The fully automatic thin layer chromatography spray box according to claim 1, characterized in that: The automatic liquid dispenser comprises a liquid dispenser body and a C-shaped guide strip, wherein: The upper and lower ends of the C-shaped guide bar are fixedly connected to the liquid extractor body, and a guide groove for accommodating the L-shaped guide plate is formed between the inner side of the C-shaped guide bar and the liquid extractor body, and a bar rack matched with a transmission gear is provided on the outer side wall.

4. The fully automatic thin layer chromatography spray box according to claim 1, characterized in that: Also includes a thin layer plate drawer and a developer drawer, wherein: The thin layer plate drawer and the developer drawer are respectively drawable and arranged in the first accommodating cavity and the second accommodating cavity.

5. The fully automatic thin layer chromatography spray box according to claim 4, characterized in that: The left and right side walls of the thin-layer board drawer are respectively provided with first observation windows, the upper part of the rear plate is provided with a second observation window, and a plurality of thin-layer board supporting columns are arranged inside.

6. The fully automatic thin layer chromatography spray box according to claim 5, characterized in that: The left and right side walls of the developer drawer are respectively provided with third observation windows, the upper part of the rear plate is provided with a fourth observation window, and a reagent support frame is arranged inside.

7. The fully automatic thin layer chromatography spray box according to claim 1, characterized in that: It also includes a controller disposed at the upper front end of the box, wherein: The controller is electrically connected to the spray mechanism and the automatic liquid taking mechanism respectively to control the spray mechanism and the automatic liquid taking mechanism to work according to instructions.

Images