Quantitative treatment device for blood culture positive sample

Abstract

The utility model relates to the technical field of biological sample detection, in particular to a quantitative treatment device for a blood culture positive sample, which comprises a treatment tank and a cleat tube mounted at the bottom of the treatment tank and communicated with an inner cavity of the treatment tank. The device has the advantages of being good in sealing performance and convenient to extract, the silica gel stirring paddle is made to make efficient contact with a positive sample in the mode that the motor drives the silica gel stirring paddle to rotate, and therefore target bacteria with negative charges in the positive sample are fully adsorbed to the silica gel stirring paddle; a positive sample entering the treatment tank is uniformly sprayed into the treatment tank from a discharge hole in a manner of rotating a rotating pipe, the mixing contact frequency with a silica gel stirring paddle is further increased, and an eluent is sprayed above an inner cavity of the treatment tank through a second liquid inlet pump and continuously rotates along with the silica gel stirring paddle; the target bacteria on the silica gel stirring paddle are fully eluted.

Description

Technical Field

[0001] The utility model relates to the technical field of biological sample detection, in particular to a blood culture positive sample quantitative processing device. Background Art

[0002] Bloodstream infection is caused by various pathogenic microorganisms and toxins invading the human body's circulating blood. Blood culture is the gold standard for BSI diagnosis and the key to clinical treatment of BSI. It can quickly and accurately determine pathogen information, and then timely use of antibiotics for symptomatic treatment. Although the standard turnaround time for isolation, identification and drug sensitivity analysis in clinical microbiology laboratories is 48 to 96 hours after a positive blood culture, many rapid detection methods can provide results within 6 to 24 hours. Most of these new rapid detection methods use blood after the blood bottle is positive as a sample, which can quickly identify and analyze drug sensitivity.

[0003] The Chinese utility model patent with the authorization announcement number "CN219757866U" is specifically a "blood culture positive sample quantitative processing device". The specific implementation method of its specification records "first use a universal venous blood collection needle to connect the positive blood culture bottle with the present invention, specifically, one end of the venous blood collection needle is inserted into the positive blood culture bottle rubber stopper, and the other end is inserted into the sampling tube from the rubber stopper on the sampling port, and then the push rod is pulled backwards. Under the suction action of the rubber piston moving backwards, the positive sample in the blood culture bottle is sucked. Note that the farthest distance the rubber piston moves backwards at this time should ensure that it does not contact the first rubber protrusion, and then the sampling tube is slightly swung in this state, so that the negatively charged target bacteria in the positive sample can be adsorbed on the silicone fan blades of the sampling head. After the target bacteria are adsorbed, the push rod can be pulled backwards to make the rubber The piston continues to slide backward and pushes the first sliding plate of the first elastic sealing component to open the liquid discharge port. At this time, the positive sample without the target bacteria will flow into the liquid discharge bin through the liquid discharge port. When all the positive samples without the target bacteria flow into the liquid discharge bin, the push rod is pulled backward continuously, so that with a little force, the resistance of the first rubber protrusion to the rubber piston is overcome, and the rubber piston slides over the first rubber protrusion. At this time, the first sliding plate will slide forward again under the rebound action of the first compression spring, and then the liquid discharge port is sealed. The push rod is pulled backward continuously, so that the rubber piston continues to slide backward and pushes the second sliding plate of the second elastic sealing component to open the liquid injection port, so that the eluent in the washing liquid bin flows into the sampling tube. At this time, the sampling tube is swung again, so that the eluent is fully in contact with the sampling head, and the target bacteria adsorbed on the silicone fan blades of the sampling head are washed off. ";

[0004] After the above-mentioned device completes the extraction of positive samples in the blood culture bottle, it is necessary to seal the sampling tube port with a rubber stopper to ensure that the interior of the sampling tube is closed. However, in the process of inserting the rubber stopper, foreign bacteria are easily mixed into the sampling tube along with air, and there is a problem of poor sealing. After the rubber stopper at the end of the sampling tube of the above-mentioned device is sealed, the interior of the sampling tube is a closed space. The positive sample in the blood culture bottle is extracted by pulling the rubber piston, which will make the extraction very difficult due to the negative pressure. The positive sample extracted by the above-mentioned device and the sampling head are slightly shaken, and it is difficult to fully adsorb the negatively charged target bacteria in the positive sample onto the sampling head. In addition, it continues to pull the rubber piston to open the injection port, and the eluent is discharged into the sampling tube and the sampling head is still mixed and contacted by a slight shaking method, and it is difficult to elute the target bacteria on the sampling head. Utility Model Content

[0005] In view of the above-mentioned shortcomings of the prior art, the utility model provides a blood culture positive sample quantitative processing device, which can effectively solve the problem of poor drying effect of the prior art.

[0006] In order to achieve the above objectives, the present invention is implemented through the following technical solutions:

[0007] The utility model provides a quantitative processing device for positive blood culture samples, comprising a processing tank and a sheep horn tube installed at the bottom of the processing tank and connected to the inner cavity of the processing tank, an adsorption component comprising a rotating tube rotatably installed in the inner cavity of the processing tank, and also comprising a plurality of silicone stirring paddles installed on the outer tube wall of the rotating tube and located at the bottom of the inner cavity of the processing tank, the outer tube wall of the rotating tube passes through the inner cavity and is provided with a plurality of drainage holes, a first liquid inlet pump is installed on the outer tank wall of the processing tank, an input end of the first liquid inlet pump is connected to a positive blood sample bottle, an output end extends into the processing tank and is connected to the inner cavity of the rotating tube, a second liquid inlet pump is installed on the outer tank wall of the processing tank, an input end of the second liquid inlet pump is connected to an eluent storage box, an output end extends into the processing tank, and is connected to an annular spray head installed at the top of the inner cavity of the processing tank.

[0008] Furthermore, a connected liquid storage box is installed at the bottom end of the processing tank, a discharge port of the liquid storage box is connected to the input end of the sheep horn tube, and two output ends of the sheep horn tube are respectively installed with a first solenoid valve and a second solenoid valve.

[0009] Furthermore, a plurality of support columns are installed at the bottom end of the processing tank and are distributed in a circumferential shape with equal intervals about the liquid storage box.

[0010] Furthermore, the adsorption assembly also includes a motor installed on the top of the processing tank, and the output end is connected to the top of the rotating tube. The motor and the outer tube wall of the rotating tube penetrate the inner cavity and are also provided with a plurality of first liquid inlet holes distributed in a ring array.

[0011] Furthermore, a connecting ring is sleeved on the outer tube wall of the rotating tube, and the inner hole wall of the connecting ring penetrates the inner cavity to open a second liquid inlet hole corresponding to the position of the first liquid inlet hole, and a first liquid inlet pipe is installed between the outer ring wall of the connecting ring and the inner cavity wall of the processing tank, and the output end of the first liquid inlet pump is connected with the inner cavity of the rotating tube through the first liquid inlet pipe and the connecting ring.

[0012] Furthermore, a second liquid inlet pipe is connected between the output end of the second liquid inlet pump and the annular spray head.

[0013] Compared with the known prior art, the technical solution provided by the utility model has the following beneficial effects:

[0014] 1. A sealed processing tank is set up to extract the positive sample from the blood sample bottle through the first liquid extraction pump. There is no need to control the opening and closing of the processing tank feed port through a rubber stopper. There will be no problem of external bacteria mixed with air entering the sampling tube to contaminate the positive sample. It has the advantage of good sealing performance and is also easy to extract;

[0015] 2. By arranging multiple silicone stirring paddles in contact with the positive samples in the processing tank and driving the silicone stirring paddles to rotate by a motor, the silicone stirring paddles can efficiently contact the positive samples, so that the negatively charged target bacteria in the positive samples are fully adsorbed on the silicone stirring paddles. The positive samples entering the processing tank first enter the rotating tube that drives the silicone stirring paddles to rotate, and are evenly sprayed in the processing tank from the discharge hole by the rotation of the rotating tube, further increasing the frequency of mixing and contact with the silicone stirring paddles. The eluent is sprayed above the inner cavity of the processing tank through the second liquid inlet pump, and the target bacteria on the silicone stirring paddles continue to rotate, so that the target bacteria on the silicone stirring paddles are fully eluted. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments or the prior art descriptions are briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model, and for ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0017] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0018] Figure 2 For the utility model Figure 1 The enlarged structural diagram at A in the middle;

[0019] Figure 3 This is a schematic diagram of the installation structure of the adsorption component of the utility model;

[0020] Figure 4It is a schematic diagram of the installation structure of the rotating tube and the connecting ring of the utility model.

[0021] The numbers in the figure represent: 1. treatment tank; 11. liquid storage box; 12. horn tube; 13. first solenoid valve; 14. second solenoid valve; 15. support column; 2. motor; 21. rotating tube; 22. silicone stirring paddle; 23. drainage hole; 24. first liquid inlet hole; 3. first liquid inlet pump; 31. connecting ring; 32. second liquid inlet hole; 33. first liquid inlet pipe; 4. second liquid inlet pump; 41. annular spray head; 42. second liquid inlet pipe. DETAILED DESCRIPTION

[0022] In order to make the purpose, technical solution and advantages of the embodiment of the utility model clearer, the technical solution in the embodiment of the utility model will be clearly and completely described below in conjunction with the drawings in the embodiment of the utility model. Obviously, the described embodiment is a part of the embodiment of the utility model, not all of the embodiments. Based on the embodiment of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0023] The utility model is further described below in conjunction with embodiments.

[0024] Example 1

[0025] Reference Figure 1-4 , which is the first embodiment of the utility model, discloses a blood culture positive sample quantitative processing device, including a processing tank 1 and a sheep horn tube 12 installed at the bottom of the processing tank 1 and connected to the inner cavity of the processing tank 1, the adsorption component includes a rotating tube 21 rotatably installed in the inner cavity of the processing tank 1, and also includes a plurality of silica gel stirring paddles 22 installed on the outer tube wall of the rotating tube 21 and located at the bottom of the inner cavity of the processing tank 1, and the outer tube wall of the rotating tube 21 is provided with a plurality of drainage holes 23 through the inner cavity, so that the positive sample is evenly sprayed in the processing tank 1, The first liquid inlet pump 3 is installed on the outer tank wall of the processing tank 1, the input end of the first liquid inlet pump 3 is connected to a positive blood sample bottle, the output end extends into the processing tank 1, and is connected to the inner cavity of the rotating tube 21, the second liquid inlet pump 4 is installed on the outer tank wall of the processing tank 1, the input end of the second liquid inlet pump 4 is connected to an eluent storage box, the output end extends into the processing tank 1, and is connected to an annular spray head 41 installed at the top of the inner cavity of the processing tank 1, the annular spray head 41 sprays eluent from a high place to fully elute the target bacteria on the surface of the silica gel stirring paddle 22.

[0026] Example 2

[0027] Reference Figure 1-4, which is the second embodiment of the utility model. This embodiment is different from the first embodiment in that: a connected liquid storage box 11 is installed at the bottom end of the processing tank 1, and the discharge port of the liquid storage box 11 is connected to the input end of the horn tube 12. The two output ends of the horn tube 12 are respectively installed with a first solenoid valve 13 and a second solenoid valve 14, which respectively control the discharge of the positive sample and the eluent. The bottom end of the processing tank 1 is installed with a plurality of support columns 15 distributed in a circular shape with equal spacing about the liquid storage box 11. The adsorption component also includes a motor 2 installed on the top of the processing tank 1 and connected to the top of the rotating tube 21 at the output end. The outer tube wall of the rotating tube 21 passes through the inner cavity and is also provided with a plurality of circular The first liquid inlet holes 24 are distributed in a shaped array, and a connecting ring 31 is rotated on the outer tube wall of the rotating tube 21. The inner hole wall of the connecting ring 31 penetrates the inner cavity and opens a second liquid inlet hole 32 corresponding to the position of the first liquid inlet hole 24, ensuring that the first liquid inlet pump 3 can still add quantitative positive samples to the inner cavity of the processing tank 1 during the rotation of the rotating tube 21. A first liquid inlet pipe 33 is installed between the outer ring wall of the connecting ring 31 and the inner cavity wall of the processing tank 1. The output end of the first liquid inlet pump 3 is connected to the inner cavity of the rotating tube 21 through the first liquid inlet pipe 33 and the connecting ring 31, and the second liquid inlet pipe 42 is connected between the output end of the second liquid inlet pump 4 and the annular spray head 41.

[0028] The remaining structures are the same as those of Example 1.

[0029] The workflow of this utility model is as follows:

[0030] First, connect the two liquid receiving ports at the two output ends of the sheep horn tube 12, close the two solenoid valves, start the first liquid inlet pump 3, extract the positive sample in the blood culture bottle through the first liquid inlet tube 33, enter the inner cavity of the connecting ring 31, and enter the inner cavity of the rotating tube 21 through the second liquid inlet hole 32 and the first liquid inlet hole 24, and then start the motor 2 to drive the rotating tube 21 to rotate. By accelerating centrifugation, the positive sample is evenly sprayed into the inner cavity of the processing tank 1 through the multiple drainage holes 23 on the rotating tube 21, and the rotating tube 21 will also drive the silica gel stirring paddle 22 at the bottom to rotate. In the process of contacting the positive sample, the silica gel stirring paddle 22 contacts the target bacteria with negative ions in the positive sample, and adsorbs the target bacteria onto the silica gel stirring paddle 22;

[0031] Secondly, after the adsorption of target bacteria is completed, the first solenoid valve 13 is opened, and the second solenoid valve 14 is kept closed to discharge the positive sample after the target bacteria are extracted. Then, the first solenoid valve 13 is closed, and the second liquid inlet pump 4 is started to discharge the eluent into the annular spray head 41 through the second liquid inlet pipe 42, and then sprayed into the processing tank 1 through the annular spray head 41. The silicone stirring paddle 22 is kept rotating so that the target bacteria on the surface of the silicone stirring paddle 22 are fully contacted and mixed with the eluent, thereby improving the elution effect of the target bacteria.

[0032] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some of the technical features therein by equivalents. These modifications or replacements will not cause the essence of the corresponding technical solutions to deviate from the protection scope of the technical solutions of the embodiments of the present invention.

Claims

1. A blood culture positive sample quantitative processing device, comprising a processing tank (1) and a sheep horn tube (12) installed at the bottom of the processing tank (1) and connected to the inner cavity of the processing tank (1), characterized in that: Also includes: An adsorption assembly comprises a rotating tube (21) rotatably mounted in the inner cavity of a processing tank (1), and also comprises a plurality of silica gel stirring paddles (22) mounted on the outer tube wall of the rotating tube (21) and located at the bottom of the inner cavity of the processing tank (1); the outer tube wall of the rotating tube (21) penetrates the inner cavity and is provided with a plurality of drainage holes (23); A first liquid inlet pump (3) is mounted on the outer tank wall of the processing tank (1); the input end of the first liquid inlet pump (3) is connected to a positive blood sample bottle, and the output end extends into the processing tank (1) and is connected to the inner cavity of the rotating tube (21); A second liquid inlet pump (4) is mounted on the outer tank wall of the processing tank (1); the input end of the second liquid inlet pump (4) is connected to an eluent storage box, and the output end extends into the processing tank (1) and is connected to an annular spray head (41) mounted at the top of the inner cavity of the processing tank (1).

2. A blood culture positive sample quantitative processing device according to claim 1, characterized in that: A communicating liquid storage box (11) is installed at the bottom end of the processing tank (1), a discharge port of the liquid storage box (11) is connected to an input end of a horn tube (12), and two output ends of the horn tube (12) are respectively installed with a first solenoid valve (13) and a second solenoid valve (14).

3. A blood culture positive sample quantitative processing device according to claim 1, characterized in that: A plurality of support columns (15) are installed at the bottom end of the processing tank (1) and are distributed in a circular pattern at equal intervals about the liquid storage box (11).

4. A blood culture positive sample quantitative processing device according to claim 1, characterized in that: The adsorption assembly also includes a motor (2) mounted on the top of the treatment tank (1) and having an output end connected to the top of the rotating tube (21); the motor (2) and the rotating tube (21) have an outer tube wall that penetrates the inner cavity and is provided with a plurality of first liquid inlet holes (24) distributed in a ring array.

5. A blood culture positive sample quantitative processing device according to claim 1, characterized in that: The outer tube wall of the rotating tube (21) is sleeved with a connecting ring (31); the inner hole wall of the connecting ring (31) penetrates the inner cavity and is provided with a second liquid inlet hole (32) corresponding to the first liquid inlet hole (24); a first liquid inlet pipe (33) is installed between the outer ring wall of the connecting ring (31) and the inner cavity wall of the processing tank (1); and the output end of the first liquid inlet pump (3) is connected to the inner cavity of the rotating tube (21) through the first liquid inlet pipe (33) and the connecting ring (31).

6. A blood culture positive sample quantitative processing device according to claim 1, characterized in that: A second liquid inlet pipe (42) is connected between the output end of the second liquid inlet pump (4) and the annular spray head (41).

Images