Device for marking renal biopsy sample and slide matched with device for use

Abstract

The utility model provides a device for marking kidney biopsy samples and a slide matched with the device for use, the device comprises a fixing part, the fixing part comprises two limiting edges which are oppositely arranged, and an accommodating space is reserved between the two limiting edges; the marking part comprises a sliding rail extending outwards along the edge of one side of the fixed part and a pointer capable of moving along the sliding rail, a sample containing space is defined by the sliding rail and the fixed part, and the end of the pointer is arranged in the sample containing space. The slide comprises a connecting part and a sample placing frame, the marking device can be connected with the connecting part through the fixing part, and the end part of the pointer is arranged above the sample accommodating part. According to the utility model, the marking device and the slide are fixed by adopting the fixing part, the number of the glomerulus is counted in the observation process of the microscope, and the position to be cut is marked through the pointer arranged on the cantilever, so that the operation of repeatedly taking and placing the slide on the microscope to separate the glomerulus is omitted, the inspection time is saved, and the inspection efficiency is improved. Meanwhile, the difficulty of identifying the tissue cutting position is reduced, and the efficiency is improved.

Description

Technical Field

[0001] The utility model belongs to the field of medical experimental equipment, in particular to a device for marking lower kidney biopsy samples and a glass slide using the device. Background Art

[0002] During the renal biopsy process, in order to obtain the most accurate test results, it is necessary to allocate a sufficient number of glomeruli for electron microscopy, immunofluorescence and light microscopy examinations. This requires that as many glomeruli as possible be collected during the renal puncture stage, and the collected glomerular tissue be divided as quickly as possible according to the number required in each experiment. In actual operation, due to the limitation of human body's tolerance, especially for patients with weak cortex and easy bleeding, the number of punctures needs to be reduced as much as possible to reduce the risk of bleeding. For patients with sclerosis or special kidney diseases, the number of glomeruli in their bodies is small and it is more difficult to obtain glomeruli. Therefore, the inspector needs to observe the obtained tissue sample under a microscope, find the glomeruli contained in the tissue, and cut the tissue according to the distribution of the glomeruli. In the existing method, the tissue collected from the puncture needle is placed on a glass slide, and then the blood vessels in the tissue are used as a reference to estimate the location of the glomerulus. Then the glass slide is removed for cutting, and then the glass slide is placed under a microscope for observation. The operation is repeated until the glomeruli in the tissue are divided according to the predetermined number. However, in this way, the tissue is exposed to the air for a long time, which affects the vitality of the sample; and the inspector is required to have a high level of basic pathological skills, the ability to operate instruments proficiently and strong spatial resolution ability, and the energy of the inspector is wasted, making the inspector easily fatigued. Utility Model Content

[0003] In view of this, the utility model aims to provide a device for marking renal biopsy samples and a glass slide used in conjunction with the device, so as to quickly mark the position of the sample to be segmented and shorten the inspection time.

[0004] In order to achieve the above object, the technical solution of the utility model is implemented as follows:

[0005] A renal biopsy sample marking device, comprising

[0006] The fixing part includes two limiting edges arranged opposite to each other, and a receiving space is left between the two limiting edges;

[0007] The marking part includes a cantilever extending outward from an edge of one side of the fixing part, the cantilever is provided with a slide groove, the slide groove has at least two sliders built in it, and each slider is provided with a pointer, the cantilever and the fixing part surround a sample holding space, and one end of the pointer is placed in the sample holding space.

[0008] Furthermore, a plurality of guide holes are opened on the inner side of one of the limiting edges, and a spring and a ball are respectively placed in each guide hole, wherein one end of the spring abuts against the bottom of the guide hole, and the other end abuts against the top of the ball.

[0009] Furthermore, a long hole is provided on the cantilever, and the long hole passes through the two side groove walls of the slide groove, and a through hole is provided on each slider, and the two ends of the through hole are respectively connected to the long holes; the pointer extends outward with a flange close to one end of the cantilever, and the end of the pointer passes through the slider through the through hole, so that the flange can rest against the side wall of the cantilever, and the end of the pointer is connected to a pressure head, and the pressure head and the flange are respectively placed on both sides of the cantilever and can clamp the cantilever.

[0010] Furthermore, a rubber pad is provided between the flange and the cantilever.

[0011] Furthermore, each pressure head is rotatably connected to the pointer via a rotating shaft, and the pressure head includes a loosening edge and a clamping edge, and the distance between the loosening edge and the axis of the rotating shaft is smaller than the distance between the clamping edge and the axis of the rotating shaft, and when the pressure head rotates, the clamping edge can be tightly pressed against the cantilever.

[0012] Furthermore, a pressing plate is fixedly provided on each pressing head, and when the pressing plate on one of the pressing heads rotates in a direction away from the other pressing head, the clamping edge on the pressing head can be tightly pressed against the cantilever.

[0013] Furthermore, the marking portion also includes a separator, and the separator is placed directly below the pointer.

[0014] Furthermore, the separator extends vertically upwards away from one side of the cantilever to form a rib.

[0015] The present application also relates to a glass slide used in conjunction with the marking device, the glass slide comprising a connecting portion and a sample placement frame, the connecting portion being capable of cooperating with the fixing portion of the above-mentioned device for marking renal biopsy samples, the connecting portion being placed between two limiting edges, the sample placement frame being placed in the sample accommodating space, the pointer being placed above the glass slide, and the end of the pointer being placed on one side of the sample placement frame, and the pointer being capable of moving along the length direction of the sample placement frame.

[0016] Compared with the prior art, the device for marking renal biopsy samples and the glass slide used therewith described in the utility model have the following advantages:

[0017] The utility model adopts a fixing part to fix the marking device and the glass slide. During the microscope observation process, the number of glomeruli is counted, and the position to be cut is marked by the pointer arranged on the cantilever, thereby eliminating the operation of repeatedly taking and placing the glass slide on the microscope to separate the glomeruli, saving inspection time, reducing the difficulty of identifying the tissue cutting position, and improving efficiency;

[0018] A pin is arranged on one limiting edge so that the other limiting edge cooperates with the pin to buckle and fix the fixing part on the glass slide;

[0019] The pointer is fixed to the cantilever at a predetermined position by arranging a pressure head and a flange at the end of the pointer, so that the slide can be removed from the microscope after observation and then cut to prevent the pointer from shifting during the movement of the slide.

[0020] A separator is used to isolate the pointer and the slide to prevent the pointer from interfering with the tissue on the slide during the movement of the pointer, which would affect the observation.

[0021] A connecting part and a sample placement frame are provided on the glass slide to reserve a connection position for the fixing part of the marking device, and to facilitate the operator to place the sample tissue on the glass slide so as to determine the position of the tissue to be cut by the pointer. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] The accompanying drawings constituting a part of the present invention are used to provide a further understanding of the present invention. The illustrative embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute an improper limitation on the present invention. In the accompanying drawings:

[0023] Figure 1 This is a schematic diagram of the sample marking device and the glass slide matching structure in Example 1;

[0024] Figure 2 This is a schematic diagram of the matching structure between the pointer and the cantilever in Example 1;

[0025] Figure 3 Schematic diagram of the explosion of the pointer part in Example 1

[0026] Figure 4 This is a schematic diagram of the structure of the fixing part in Example 1 (the pressing block part is omitted for clear display);

[0027] Figure 5 This is a schematic diagram of the internal structure of the fixed portion with a pin limiting edge in Example 1;

[0028] Figure 6 It is a schematic diagram of the slide structure;

[0029] Figure 7 This is a schematic diagram of the cooperation between the sample marking device and the glass slide in Example 2.

[0030] Description of reference numerals:

[0031] 1-fixed part; 11-limiting edge; 12-guide hole; 13-pin; 14-spring; 15-pressing block; 16-accommodating space; 2-marking part; 21-cantilever; 22-slide; 23-long hole; 24-pointer; 241-flange; 25-slider; 251-through hole; 26-pressing head; 261-pressing sheet; 262-relaxing edge; 263-clamping edge; 27-rubber pad; 28-separator; 29-retaining edge; 3-glass slide; 31-sample placement frame; 32-connecting part. DETAILED DESCRIPTION

[0032] It should be noted that, in the absence of conflict, the embodiments of the present invention and the features in the embodiments can be combined with each other.

[0033] In the description of the present utility model, it should be understood that the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present utility model. In addition, the terms "first", "second", etc. are only used for descriptive purposes, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Therefore, features defined as "first", "second", etc. may explicitly or implicitly include one or more of the features. In the description of the present utility model, unless otherwise specified, "multiple" means two or more.

[0034] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installation", "connection" and "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be an indirect connection through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood by specific circumstances.

[0035] The present invention will be described in detail below with reference to the accompanying drawings and in combination with embodiments.

[0036] Example 1

[0037] The renal biopsy sample marking device of the utility model comprises a fixing part 1 and a marking part 2, wherein the fixing part 1 comprises two limiting edges 11 arranged opposite to each other, and a receiving space 16 is left between the two limiting edges 11. During operation, a glass slide 3 with a renal puncture sample tissue can be placed in the receiving space 16, and the two limiting edges 11 can be stuck on the side of the glass slide 3. In this embodiment, a plurality of guide holes 12 are opened on one of the limiting edges 11, and a spring 14 and a ball 13 are respectively placed in each guide hole 12. The spring 14 and a ball 13 are respectively placed in each guide hole 12. One end of 14 abuts against the bottom of the guide hole 12, and the other end abuts against the pin 13, so that the operator can more conveniently clamp the fixing part 1 and the glass slide 3. For the convenience of production, the end edge of the guide hole 12 on the side of the accommodating space 16 extends inward to form a closing, so that the diameter of the closing position is smaller than the diameter of the guide hole 12 as a whole. In this embodiment, the pin 13 is a steel ball, and the diameter of the steel ball is larger than the closing diameter to prevent the steel ball from falling out of the guide hole 12. Optionally, the pin 13 can also be a columnar structure or a wedge-shaped structure. In order to facilitate installation, the guide hole 12 is a blind hole. An installation window is provided on the lower surface of the limiting edge 11. The installation window is connected to the guide hole 12. The assembler can place the pin 13 and the spring 14 in the guide hole 12 through the installation window, and embed the pressure block 15 in the installation window to fix the position of the spring 14 and prevent the spring 14 from accidentally popping out of the installation window. The installation of the pressure block 15 can be fixed by interference fit plug-in installation, or a fixing buckle can be provided on the installation window. Those skilled in the art can determine the specific installation dimension tolerance according to the prior art, which will not be repeated here. The marking portion 2 includes a cantilever 21 extending outward from the edge of one side of the fixing portion 1. In this embodiment, the cantilever 21 and the pin 13 are arranged on the same side of the fixing portion 1. A slide groove 22 is provided on the cantilever 21. At least two sliders 25 are built in the slide groove 22. A pointer 24 is provided on each slider 25. The cantilever 21 and the fixing portion 1 surround a sample accommodation space, and one end of the pointer 24 is placed in the sample accommodation space. In this embodiment, a long hole 23 is formed on the cantilever 21, and the long hole 23 passes through the groove walls on both sides of the slide groove 22. A through hole 251 is formed on the slider 25, and the two ends of the through hole 251 are respectively connected to the long hole 23. The pointer 24 passes through the slider 25 and the cantilever 21 through the long hole 23 and the through hole 251. The width of the end of the pointer 24 close to the sample holding space 1 is smaller than the width of the end close to the cantilever 21, so as to improve the reliability of the connection between the pointer 24 and the slider 25. A flange 241 extends outward from the end of the pointer 24 close to the cantilever 21. The end of the pointer 24 that passes through the cantilever 21 is rotatably connected to a pressure head 26, so that the flange 241 can be against the side wall of the cantilever 21. The end of the pointer 24 is connected to the pressure head 26, and the pressure head 26 and the flange 241 are respectively placed on both sides of the cantilever 21 and can clamp the cantilever 21. The cantilever 21 is clamped by the pressure head 26 and the flange 241 so that the pointer 24 can be fixed at a predetermined position.In this embodiment, each of the pressure heads 26 is rotatably connected to the pointer 24 via a rotating shaft, and the pressure head 26 includes a loose edge 262 and a clamping edge 263, and the distance between the loose edge 262 and the axis of the rotating shaft is smaller than the distance between the clamping edge 263 and the axis of the rotating shaft, and when the pressure head 26 rotates, the clamping edge 263 can be tightly against the cantilever 21, and each pressure head 26 is respectively fixed with a pressing piece 261, so that the operator can rotate the pressure head 26. When the pressure on one of the pressure heads 26 is When the sheet 261 rotates in a direction away from the other pressing head 26, the clamping edge 263 on the pressing head 26 can be pressed against the cantilever 21. In this way, when the two pressing heads 26 are pressed against the cantilever 21, the two pressing sheets 261 can be oriented in directions away from each other, preventing the two pointers 24 from being spaced too close together when the number of glomeruli required is small or the local distribution of glomeruli is relatively dense, resulting in interference between the two pressing sheets 261 or between the pressing sheet 261 and the other pressing head 26, causing the pointer 24 to be unable to be fixed. In other optional embodiments, a thread can also be provided on the portion of the pointer 24 after it passes through the cantilever 21, so that the pressing head 26 is screwed to the pointer 24, and the cantilever 21 is clamped by rotating the pressing head 26 and cooperating with the flange 241. A rubber pad 27 is provided between the flange 241 and the cantilever 21. The rubber pad 27 has a hole through which the end of the pointer 24 can pass, thereby increasing the friction between the pointer 24 and the cantilever 21 after the pressing block 15 and the flange 241 clamp the cantilever 21. At the same time, the elasticity of the rubber pad 27 makes it easier for the operator to pull the pressing plate 261 to rotate the pressing block 15.

[0038] The utility model also relates to a glass slide 3 used in conjunction with the marking device, the glass slide 3 comprising a connecting portion 32 and a sample placement frame 31, the connecting portion 32 can cooperate with the fixing portion 1 of the above-mentioned device for marking renal biopsy samples, the connecting portion 32 is placed between two limiting edges 11, the sample placement frame 31 is placed in the sample accommodating space, the pointer 24 is placed above the glass slide 3, and the end of the pointer 24 is placed on one side of the sample placement frame 31, preferably, the length of the sample placement frame 31 is less than or equal to the length of the slide 22, and the pointer 24 moves along the length direction of the sample placement frame 31. Optionally, the sample placement frame 31 is a colored rectangular marking line drawn on the pick, or a rectangular scratch directly engraved on the glass slide. The width of the rectangle is smaller than the diameter of the objective lens and larger than the caliber of the puncture needle, so that the width of the sample in the sample placement frame 31 can be completely exposed to the field of view of the microscope, and at the same time it is convenient for the sample in the puncture needle to be placed in the sample placement frame. The length of the rectangle is larger than the length of the puncture needle, so that the operator can place the sample tissue obtained by puncture into the sample placement frame. For example, for a puncture needle with a caliber of 1.2 mm and a length of 18 mm, the size of the sample placement frame can be set to 3 mm*18 mm.

[0039] The renal biopsy sample marking device of the utility model is used to transfer the sample tissue obtained by puncture into the sample placement frame 31 of the glass slide 3 during use. During the transfer process, the tissue that is deviated from the position can be moved into the sample placement frame 31 by forceps or a puncture needle, and then the limiting edge 11 without the pin 13 is pressed against the edge of the connecting portion 32 of the glass slide 3 to press the marking device downward. During the movement of the marking device, the pin 13 first contacts the edge of the glass slide 3 and is pressed into the guide hole 12. The spring 14 applies an external force to the pin 13 to clamp the glass slide 3 through the pin 13 and the limiting edge 11 to prevent the marking device from deviating from the glass slide 3 during the movement of the pointer 24. In the initial stage, the two pointers 24 can be placed at the two ends of the slide 22 respectively to move the glass slide 3 and the guide hole 12. The marking device is placed as a whole at a predetermined position of the microscope so that one end of the sample placement frame 31 corresponds to the objective lens of the microscope. The sample tissue in the sample placement frame 31 is observed through the microscope. The glass slide 3 is moved on the microscope during the observation. When the number of glomeruli passing through the field of view of the objective lens reaches a predetermined number, one of the pointers 24 is moved to this position, and the pressure head 26 is rotated to fix the pointer 24. During this process, the operator can observe whether the end of the pointer 24 has moved into place through the field of view provided by the objective lens, and then repeat the above operation to move the other pointer 24 to the predetermined position and lock it. At this time, the glomeruli in the sample tissue are divided into three parts according to the required number through the marked positions of the two pointers 24. Finally, the glass slide 3 is removed and the sample tissue is cut with a special blade.

[0040] Example 2

[0041] On the basis of Example 1, the marking portion 2 also includes a separator 28, which is placed directly below the pointer 24. In this embodiment, the separator 28 is integrally formed with the cantilever 21 and the fixing portion 1. The end of the separator 28 also extends vertically upward to form a retaining edge 29, and the retaining edge 29 is placed on the edge of the sample placement frame 31. During operation, the marking device and the glass slide 3 can be fixed first so that the separator 28 is placed between the pointer 24 and the glass slide 3, and then the sample tissue is moved onto the glass slide 3. The retaining edge 29 prevents the sample tissue from falling onto the separator 28. While making it easier to place the sample tissue at the specified position on the glass slide 3, it prevents the pointer 24 from interfering with the sample tissue during movement and affecting the position of the glomerulus.

[0042] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A device for labeling a renal biopsy sample, characterized in that: include The fixing part includes two limiting edges arranged opposite to each other, and a receiving space is left between the two limiting edges; The marking part includes a cantilever extending outward from an edge of one side of the fixing part, the cantilever is provided with a slide groove, the slide groove has at least two sliders built in it, and each slider is provided with a pointer, the cantilever and the fixing part surround a sample holding space, and one end of the pointer is placed in the sample holding space.

2. A device for renal biopsy sample labeling according to claim 1, characterized in that: A plurality of guide holes are opened on the inner side of one of the limiting edges, and a spring and a ball are respectively arranged in each guide hole. One end of the spring abuts against the bottom of the guide hole, and the other end abuts against the top of the ball.

3. The device for renal biopsy sample labeling according to claim 1, characterized in that: The cantilever is also provided with a long hole, and the long hole passes through the two side groove walls of the slide groove. Each slider is provided with a through hole, and the two ends of the through hole are respectively connected to the long hole; the pointer extends outward with a flange close to one end of the cantilever, and the end of the pointer passes through the slider through the through hole, so that the flange can be against the side wall of the cantilever. The end of the pointer is connected to a pressure head, and the pressure head and the flange are respectively placed on both sides of the cantilever and can clamp the cantilever.

4. A device for renal biopsy sample labeling according to claim 3, characterized in that: A rubber pad is provided between the flange and the cantilever.

5. The device for renal biopsy sample labeling according to claim 3, characterized in that: Each pressure head is rotatably connected to the pointer via a rotating shaft, and the pressure head comprises a loosening edge and a clamping edge, and the distance between the loosening edge and the axis of the rotating shaft is smaller than the distance between the clamping edge and the axis of the rotating shaft, and when the pressure head rotates, the clamping edge can be tightly pressed against the cantilever.

6. The device for renal biopsy sample labeling according to claim 3, characterized in that: A pressing sheet is fixedly arranged on each pressing head, and when the pressing sheet on one pressing head rotates in a direction away from the other pressing head, the clamping edge on the pressing head can be tightly pressed against the cantilever.

7. The device for renal biopsy sample labeling according to claim 1, characterized in that: The marking portion further comprises a separator, and the separator is placed just below the pointer.

8. The device for renal biopsy sample labeling according to claim 7, characterized in that: The separator extends vertically upwards away from one side of the cantilever to form a retaining edge.

9. A slide glass, characterized in that: It comprises a connecting part and a sample placement frame, wherein the connecting part can be placed in the accommodating space of the fixing part of the device for marking renal biopsy samples according to any one of claims 1 to 6, the sample placement frame is placed in the sample placement frame, the pointer is placed above the glass slide, and the end of the pointer is placed on one side of the sample placement frame, and the pointer can move along the length direction of the sample placement frame.

10. A slide glass according to claim 9, characterized in that: The marking part comprises a separator, and the separator is arranged between the paddle and the pointer, and a retaining edge is extended upward from one side of the separator away from the cantilever, and the retaining edge is arranged at the edge of the sample placement frame.

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