Plant column chromatography purification equipment

Abstract

The utility model relates to the technical field of chromatography, and discloses plant column chromatography purification equipment which comprises an upper-section column body and a lower-section column body, the upper-section column body and the lower-section column body are detachably connected, a separator is arranged between the upper-section column body and the lower-section column body, the separator is used for separating filler in the upper-section column body and filler in the lower-section column body, and the upper-section column body and the lower-section column body are detachably connected. And the separator is a second filter screen, and the second filter screen is arranged at the bottom of the upper-section column body. According to the plant column chromatography purification equipment, the upper section column body is internally provided with the upper sample layer filler, the lower section column body is internally provided with the separation layer filler, the upper section column body and the lower section column body form a split type chromatographic column, the upper section column body is detached after elution of each batch of samples is finished, and the upper sample layer filler and the separation layer filler are separated by a second filter screen in the upper section column body, so that the separation layer filler is separated from the separation layer filler; according to the utility model, an operator can independently take out and replace the loading layer filler, so that the loading layer filler and the separation layer filler can be completely separated, and the column layer separation effect of each batch is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of chromatography, in particular to a plant column chromatography purification device. Background Art

[0002] Chromatography is a technique that uses the differences in the physical and chemical properties of different substances. Column chromatography is a form of chromatography in which a mixture is separated by a columnar filler and is used to separate and purify compounds from a mixture. Plant column chromatography purification is a technical method used to separate and purify compounds from plant extracts. This method uses column chromatography to separate and purify target compounds through differences in the adsorption, distribution and elution characteristics of different compounds on fixed fillers. When performing plant column chromatography, the process of introducing the sample into the column is called loading, and there are two main methods of loading: dry loading and wet loading.

[0003] During the dry loading process, the loading layer absorbs a lot of impurities and is prone to dead adsorption. After each batch of loading and elution, the operator needs to manually remove the adsorbent filler in the loading layer before loading the next batch. However, the fillers in the loading layer and the separation layer are in contact with each other. When removing the adsorbent filler in the loading layer, it is difficult to effectively separate the two fillers completely. The loaded loading layer filler is difficult to achieve uniformity each time, resulting in differences in the column layer separation effect of each batch, and the column chromatography separation effect of the product between batches is not stable enough. Utility Model Content

[0004] The purpose of the utility model is to provide a plant column chromatography purification device to solve the problems raised in the above background technology.

[0005] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a plant column chromatography purification equipment, comprising: an upper column section and a lower column section, the upper column section and the lower column section are detachably connected, a partition is arranged between the upper column section and the lower column section, and the partition is used to separate the filler in the upper column section and the filler in the lower column section.

[0006] Preferably, the separator is a second filter.

[0007] Preferably, the second filter is arranged at the bottom of the upper column.

[0008] Preferably, the upper section of the column is detachably connected to a first head.

[0009] Preferably, the first end cover is connected to a feeding mechanism.

[0010] Preferably, the feeding mechanism comprises a liquid inlet pipe, and the liquid inlet pipe is fixedly mounted on the first head.

[0011] Preferably, the liquid inlet pipe is connected to the upper column.

[0012] Preferably, a regulating valve is provided on the liquid inlet pipe.

[0013] Preferably, the lower column is provided with a liquid outlet, and a valve is provided at the liquid outlet.

[0014] Preferably, a first filter screen is disposed in the lower cylinder, and the first filter screen is located above the liquid outlet.

[0015] The plant column chromatography purification equipment has the following beneficial effects:

[0016] The plant column chromatography purification equipment has a loading layer filler arranged in an upper column body and a separation layer filler arranged in a lower column body. The upper column body and the lower column body form a split chromatography column. After elution of each batch of loading, the upper column body is disassembled. Since the second filter screen in the upper column body separates the loading layer filler from the separation layer filler, an operator can take out and replace the loading layer filler separately, which is convenient for completely separating the loading layer filler from the separation layer filler, thereby improving the column layer separation effect of each batch. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 A three-dimensional diagram of a plant column chromatography purification device provided in an embodiment of the present application;

[0018] Figure 2 for Figure 1 Schematic diagram of the mid-layer chromatography column;

[0019] Figure 3 for Figure 2 Exploded view of the mid-layer chromatography column;

[0020] Figure 4 for Figure 2 A cross-sectional view of a mid-layer chromatography column;

[0021] Figure 5 for Figure 3 Bottom view of the middle and upper section of the column;

[0022] Figure 6 is a schematic diagram of the lower column in a clean state;

[0023] Figure 7 for Figure 6 Cross-sectional view of the middle and lower sections of the column.

[0024] In the figure: 1. bracket; 2. lower section of the cylinder; 21. liquid outlet; 22. first filter screen; 3. upper section of the cylinder; 31. second filter screen; 4. first end cap; 41. liquid inlet pipe; 42. regulating valve; 5. quick release clamp; 6. second end cap; 61. motor; 62. cleaning brush. DETAILED DESCRIPTION

[0025] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0026] like Figure 1-Figure 2 As shown, the utility model provides a plant column chromatography purification device, comprising: a bracket 1, the bracket 1 is detachably connected with a chromatography column, the chromatography column comprises an upper column 3 and a lower column 2, the upper column 3 and the lower column 2 are detachably connected to form a split chromatography column, the connecting piece between the upper column 3 and the lower column 2 is a quick-release clamp 5, the filler in the upper column 3 forms a sample loading layer, and the filler in the lower column 2 forms a separation layer.

[0027] Specifically, Figure 3-Figure 5 As shown, a partition is provided between the upper column 3 and the lower column 2, and the partition is used to separate the filler in the upper column 3 and the filler in the lower column 2. The partition includes but is not limited to a filter screen and a filter cloth. Preferably, the partition is a filter screen, i.e., a second filter screen 31. The second filter screen 31 is provided at the bottom of the upper column 3. The second filter screen 31 separates the upper layer filler from the separation layer filler to avoid contact and adhesion between the two fillers, and also allows the eluent to flow downward through the second filter screen 31.

[0028] Among them, the upper section column 3 is detachably connected to the first head 4 through a quick-release clamp 5, and a seal is arranged between the upper section column 3 and the first head 4. The first head 4 is connected to a feeding mechanism, and the feeding mechanism includes a liquid inlet pipe 41, and the liquid inlet pipe 41 is fixedly installed on the first head 4, and the liquid inlet pipe 41 is connected to the upper section column 3. One end of the liquid inlet pipe 41 is connected to an external liquid supply system, and a regulating valve 42 is arranged on the liquid inlet pipe 41, and the outlet of the eluent is adjusted by the regulating valve 42.

[0029] Furthermore, a liquid outlet 21 is formed in the lower section of the column 2, and a valve (not shown in the figure) is arranged at the liquid outlet 21. A first filter screen 22 is arranged in the lower section of the column 2, and the first filter screen 22 is located above the liquid outlet 21, so that the solution can pass through the first filter screen 22 and flow out of the liquid outlet 21 while preventing the separation layer filler from falling.

[0030] The inventors have found that in the prior art, when adding materials to a chromatography column, the eluent is generally poured directly into the top of the chromatography column. This method of adding materials easily causes external impurities to enter the interior of the chromatography column.

[0031] In this embodiment, when adding eluent to the chromatography column, the regulating valve 42 can be opened, and the eluent provided by the external liquid supply system flows into the upper column 3 through the liquid inlet pipe 41. The eluent penetrates into the upper layer filler and slowly flows downward and flows along the inner wall of the chromatography column. The difference in affinity of different compounds in the product on the separation layer filler in the lower column 2 will cause them to move at different rates to achieve separation. According to the migration speed of the separated substance, solutions of different components are collected one by one at the liquid outlet 21 to finally obtain the purified target substance.

[0032] It can be understood that after the elution of each batch of samples is completed, the upper column 3 is disassembled. Since the second filter 31 in the upper column 3 separates the loading layer filler from the separation layer filler, the operator can take out and replace the loading layer filler separately, which is convenient for completely separating the loading layer filler from the separation layer filler, thereby improving the column layer separation effect of each batch.

[0033] like Figure 6-Figure 7 As shown, the plant column chromatography purification equipment also includes a second head 6, which is detachably connected to the lower column 2, and the connecting piece is a quick-release clamp 5. The second head 6 is connected to a cleaning mechanism, and the cleaning mechanism is used to clean the lower column 2. The cleaning mechanism includes a motor 61, and the motor 61 is fixedly installed on the top of the second head 6 through a motor frame. A cleaning brush 62 is fixedly installed at the output end of the motor 61, and the cleaning brush 62 passes through the second head 6 and extends into the lower column 2, and the cleaning brush 62 is rotatably connected to the second head 6 through a bearing, and the brush rod of the cleaning brush 62 adopts a telescopic brush rod, which can be adjusted according to the length of the lower column 2.

[0034] In the present application, when the lower section column 2 needs to be cleaned after the operation is completed, the upper section column 3 is disassembled and manually cleaned. Since the lower section column 2 is a long hollow cylinder with a long length, it is difficult to manually clean it after use. The bottom of the inner wall of the lower section column 2 is prone to cleaning dead corners. Therefore, after the separation layer filler is taken out from the lower section column 2, the second head 6 is fixed on the lower section column 2, and the motor 61 is turned on. The motor 61 drives the cleaning brush 62 to rotate, and the rotating cleaning brush 62 cleans the inner wall of the lower section column 2.

[0035] Working principle: When loading by dry method, the product is first dissolved in a solvent, and then the solvent is removed by evaporation or drying to deposit the product on a solid adsorbent to form a dry powder. The powder containing the product is then evenly loaded into the upper column 3 to form a loading layer filler. The upper column 3 is installed on the lower column 2 containing the separation layer filler, and then an eluent is added to the chromatography column. The regulating valve 42 is opened, and the eluent provided by the external liquid supply system flows into the upper column 3 through the liquid inlet pipe 41, and the eluent penetrates into the loading layer filler. The liquid slowly flows downward and along the inner wall of the chromatography column. The difference in affinity of different compounds in the product on the separation layer filler in the lower column 2 will cause them to move at different rates to achieve separation. According to the migration speed of the separated substance, solutions of different components are collected one by one at the liquid outlet 21 to finally obtain the purified target substance. After each batch of sample loading and elution, the sample layer filler needs to be taken out, and the upper column 3 is disassembled by the quick-release clamp 5. The sample layer filler in the upper column 3 is taken out separately and replaced, and then the next batch of sample loading operation is carried out.

[0036] In summary, although the embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and variations may be made to these embodiments without departing from the principles and spirit of the present invention, and the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A plant column chromatography purification device, characterized in that: include: An upper column section (3) and a lower column section (2), wherein the upper column section (3) and the lower column section (2) are detachably connected, and a partition is provided between the upper column section (3) and the lower column section (2), and the partition is used to separate the filler in the upper column section (3) and the filler in the lower column section (2).

2. A plant column chromatography purification device according to claim 1, characterized in that: The separator is a second filter screen (31).

3. A plant column chromatography purification device according to claim 2, characterized in that: The second filter screen (31) is arranged at the bottom of the upper column (3).

4. A plant column chromatography purification device according to claim 1, characterized in that: The upper column (3) is detachably connected to a first sealing head (4).

5. A plant column chromatography purification device according to claim 4, characterized in that: The first sealing head (4) is connected to a feeding mechanism.

6. A plant column chromatography purification device according to claim 5, characterized in that: The feeding mechanism comprises a liquid inlet pipe (41), and the liquid inlet pipe (41) is fixedly mounted on the first sealing head (4).

7. A plant column chromatography purification device according to claim 6, characterized in that: The liquid inlet pipe (41) is connected to the upper column (3).

8. A plant column chromatography purification device according to claim 7, characterized in that: The liquid inlet pipe (41) is provided with a regulating valve (42).

9. A plant column chromatography purification device according to claim 1, characterized in that: The lower column (2) is provided with a liquid outlet (21), and a valve is arranged at the liquid outlet (21).

10. A plant column chromatography purification device according to claim 9, characterized in that: A first filter screen (22) is arranged in the lower column (2), and the first filter screen (22) is located above the liquid outlet (21).

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