Equipment for biological purification

By adopting a low voltage drop circuit structure in the magnetic bead purification equipment, the loss problem during voltage transmission is solved, the force output of the magnetic rod sleeve and the magnetic rod is ensured, and the effective mixing of the reaction liquid in the porous reagent kit is achieved.

CN222356147UActive Publication Date: 2025-01-14KANGMA (SHANGHAI) BIOTECH LTD
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Patent Information

Application Number
CN202323666797.2
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2023-12-29
Publication Date
2025-01-14
Estimated Expiration
2033-12-29

AI Technical Summary

Technical Problem

When the existing magnetic bead purification equipment deals with porous reagent kits with partition layers, due to the voltage drop problem during voltage transmission, the force output of the magnetic rod sleeve and magnetic rod is insufficient, and the partition layer cannot be effectively broken down, causing the reaction liquid. Unable to mix and meet purification requirements.

Method used

A low voltage drop circuit structure is designed. Through the design of different wire diameters of power lines and cables and independent motor power interfaces, voltage transmission loss is reduced, and the output voltage of the working motor reaches 24V, meeting the strength requirements of the purification equipment.

Benefits of technology

It realizes that the voltage transmission loss is reduced without replacing the large torque working motor, ensuring that the magnetic rod sleeve and the magnetic rod can effectively break the partition layer, realize the mixing of the reaction liquid, and meet the actual purification needs.

✦ Generated by Eureka AI based on patent content.

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Abstract

The utility model provides equipment for biological purification, which comprises a circuit module, the circuit module is provided with a PCB (printed circuit board), the PCB is provided with at least one motor power supply interface and a power strip interface, working motors are connected with the corresponding motor power supply interfaces through respective power lines, and the power lines are connected with the power strip interface. And all the sensors are connected with the power strip interface through respective flat cables. According to the utility model, under the conditions that a high-power working motor does not need to be replaced and a whole set of purification structure needs to be redesigned in order to be matched with the high-power working motor, the loss in the voltage transmission process can be reduced only through a low-voltage-drop circuit structure, and the actual purification requirement is met.
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Description

Technical Field

[0001] The utility model relates to the field of purification equipment, in particular to equipment used for biological purification. Background Art

[0002] A working motor, commonly known as a "motor", refers to an electromagnetic device that realizes the conversion or transmission of electrical energy according to the law of electromagnetic induction. Its main function is to generate driving torque as a power source for electrical appliances or various machines. In existing magnetic bead purification equipment, the force output of the magnetic rod and the magnetic rod sleeve is provided by the working motor. When it comes to porous test kits with a partition layer, the purification equipment needs to rely on the magnetic rod sleeve and the magnetic rod to break the partition layer so that the reaction liquids in the two reaction chambers separated by the partition layer can be mixed to complete the subsequent purification process, which increases the force output requirements of the magnetic rod and the magnetic rod sleeve.

[0003] However, there is an obvious voltage drop problem in the current actual use process: the output voltage and current of the working motor are measured by an oscilloscope. The test results show that the current is consistent with the set value, the voltage power supply end outputs 24V, and the working motor end inputs 21V, causing the power output of the working motor to drop by about 12.5% ​​compared with the set value, which cannot meet the actual purification needs. The most direct impact of the above voltage drop is that the output force of the magnetic rod sleeve and the magnetic rod becomes smaller, resulting in the inability of the purification equipment to penetrate the partition layer in the porous reagent kit under the design structure framework of the original equipment, making it impossible for the reaction liquids in the two reaction chambers to mix and react. Utility Model Content

[0004] The technical problem to be solved by the utility model is to provide a device for biological purification, which can reduce the loss in the voltage transmission process and meet the actual purification needs only through the low voltage drop circuit structure of the application without replacing the high-torque working motor and redesigning the entire purification structure to match the high-torque working motor.

[0005] In order to solve the above technical problems, the utility model provides a device for biological purification, including: a circuit module, the circuit module has a PCB circuit board, the PCB circuit board is provided with at least one motor power interface and a socket strip interface, the working motors are connected to the corresponding motor power interfaces through their respective power lines, and all sensors are connected to the socket strip interfaces through their respective cables.

[0006] Specifically, the wire diameter of the power wire is larger than the wire diameter of the flat cable.

[0007] Specifically, the wire diameter of the power line is 1 square millimeter, and the wire diameter of the flat cable is 0.3 square millimeter.

[0008] Specifically, the PCB circuit board supplies a 24V power supply to the working motor, and the output current of the working motor is 2.2A.

[0009] Specifically, the working motor can drive the corresponding magnetic rod to be inserted into the corresponding magnetic rod sleeve, drive the magnetic rod and the magnetic rod sleeve to move downward to the specified position in the sleeve state, and move back and forth vertically according to the set cavity breaking force and cavity breaking times to break the separation layer in the porous reagent box, so that the reaction liquids in the two reaction chambers separated by the separation layer are mixed.

[0010] Specifically, the biological purification equipment also includes: a moving mechanism for driving the magnetic rod sleeve and / or the magnetic rod to move vertically and / or horizontally. Further, the moving mechanism includes a horizontal moving mechanism and a vertical moving mechanism. Further, the moving mechanism includes a horizontal moving mechanism for driving the magnetic rod and the magnetic rod sleeve to move horizontally and a first vertical moving mechanism for driving the magnetic rod to move vertically respectively and a second vertical moving mechanism for driving the magnetic rod sleeve to move vertically.

[0011] Specifically, the first vertical moving mechanism is a screw transmission structure, the second vertical moving mechanism is a screw transmission structure, and the horizontal moving mechanism is a pulley transmission structure.

[0012] Specifically, the biological purification device also includes: a frame, and a shell surrounding the frame, wherein:

[0013] The frame is a rectangular frame, the PCB circuit board is arranged at the rear part of the frame, and a pair of first extension parts and second extension parts extending forward are symmetrically fixed on the left and right frames of the front side of the frame facing the user, the second extension part is longer than the first extension part, and the second extension part on the same frame is parallel to the first extension part and is located below the first extension part;

[0014] The outer shell includes a mounting front shell, which has a first opening and a second opening parallel to each other for mounting a display screen and a hatch, and both sides of the mounting front shell are fitted in contact with the first extension portion and the second extension portion and are obliquely mounted on the frame, and the upper rear side of the mounting front shell is fitted in contact with the upper front end side of the frame.

[0015] Specifically, the bio-purification equipment also includes any one or more of the following:

[0016] A magnetic bar assembly, including a magnetic bar holder for mounting the magnetic bar;

[0017] The magnetic rod sleeve assembly comprises a magnetic rod sleeve frame for installing the magnetic rod sleeve, the magnetic rod sleeve frame is located below the magnetic rod frame, and each magnetic rod can enter the corresponding magnetic rod sleeve;

[0018] A placement component, used for placing at least one multi-porous reagent box, wherein the placement component is located below the magnetic rod sleeve rack, and each of the magnetic rod sleeves can be correspondingly inserted into the corresponding multi-porous reagent box;

[0019] A studio, used for arranging the magnetic bar assembly, the magnetic bar sleeve assembly and the placement assembly;

[0020] A moving mechanism, used to drive the magnetic rod sleeve and / or the magnetic rod to move vertically and / or horizontally, further, the moving mechanism includes a horizontal moving mechanism and a vertical moving mechanism, further, the moving mechanism includes a horizontal moving mechanism that drives the magnetic rod and the magnetic rod sleeve to move horizontally and a first vertical moving mechanism that drives the magnetic rod to move vertically and a second vertical moving mechanism that drives the magnetic rod sleeve to move vertically;

[0021] A circuit module, including a circuit structure integrated board for controlling the purification equipment to implement the purification operation;

[0022] A heat dissipation device, used for dissipating heat from the working room;

[0023] A disinfection device is used to disinfect the working room.

[0024] Specifically, the placement assembly further includes a locking structure, which includes: a positioning portion, a connecting rod, a rotation fixing portion and a pushing portion, wherein:

[0025] One end of the connecting rod is rotatably connected to the pushing part, and the other end is rotatably connected to the rotating fixing part;

[0026] The positioning portion is used to be fixed on the base;

[0027] The rotating fixing part is sleeved on the positioning part and can be driven by the connecting rod to rotate on the positioning part;

[0028] The pushing part is used to push the connecting rod to drive the rotating fixing part:

[0029] When the pushing part pushes the connecting rod, the connecting rod drives the rotating fixing part to rotate to a suitable position to separate from the porous reagent box.

[0030] When the pushing part drives the connecting rod to reset, the connecting rod drives the rotating fixing part to rotate to a suitable position to abut against the multi-porous reagent box.

[0031] Specifically, the placement component also includes a limit member, which is used to fix and limit the porous test kit. The side of the limit member close to the porous test kit is also provided with at least one elastic clamping member installation groove for installing an elastic clamping member. The elastic clamping member is used to clamp the porous test kit. Preferably, the elastic clamping member is a marble.

[0032] Specifically, the single-side distance between the limiter and the porous reagent box is not greater than 0.7 mm. Preferably, the single-side distance between the limiter and the porous reagent box is not greater than 0.5 mm. More preferably, the single-side distance between the limiter and the porous reagent box is not greater than 0.2 mm.

[0033] Beneficial effects of the utility model:

[0034] (1) It is possible to reduce the loss in the voltage transmission process by only using the low voltage drop circuit structure of the present application without replacing the high-power working motor or redesigning the entire purification structure to match the high-power working motor, thereby meeting the actual purification needs;

[0035] (2) Separate the motor power interface of the working motor and do not use the same socket interface as the cable to cause mutual interference;

[0036] (3) Use a power cord to directly connect the motor power interface to shorten the length of the connection between the power supply and the working motor to reduce the loss during voltage transmission;

[0037] (4) The original adapter board and corresponding terminals are omitted, and the power cord is much thicker than the cable, so the loss during voltage transmission is less. BRIEF DESCRIPTION OF THE DRAWINGS

[0038] In order to more clearly illustrate the technical solution of the utility model, the following briefly introduces the drawings required for use in the utility model. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0039] Figure 1 It is the connection relationship between the working motor and the sensor on the PCB circuit board of the utility model;

[0040] Figure 2 It is the connection relationship between the working motor and the sensor on the existing PCB circuit board;

[0041] Figure 3 It is a schematic diagram of the mobile mechanism of the purification device of the utility model;

[0042] Figure 4It is a schematic diagram of the framework of the purification device of the utility model;

[0043] Figure 5 It is a schematic diagram of the structure of the purification equipment of the utility model after the side shell is removed;

[0044] Figure 6 It is a schematic diagram of the position of the locking structure of the purification equipment of the utility model when in use;

[0045] Figure 7 It is a schematic diagram of the locking structure of the purification device of the utility model;

[0046] Figure 8 It is a schematic diagram of the components of the locking structure of the purification equipment of the utility model;

[0047] Fig. 9 It is a side view and a top view of the limiting component of the purification equipment of the utility model when in use.

[0048] Among them, the reference numerals in the figure are:

[0049] 0. Multi-well kit,

[0050] 1. Shell, 11. Install the front shell,

[0051] 2. Frame, 21. First extension, 22. Second extension,

[0052] 3. Magnetic bar assembly,

[0053] 4. Magnetic rod sleeve assembly,

[0054] 5. Placement assembly, 51. Locking structure; 511. Positioning part, 512. Rotation fixing part, 513. Connecting rod, 514. Pushing part, 52. Limiting part, 521. Elastic clamping part;

[0055] 6. Circuit module, 61. Working motor, 62. Power cord, 63. Motor power interface, 64. Sensor, 65. Cable, 66. Adapter board, 67. Socket interface,

[0056] 7. Horizontal moving mechanism, 71. Third motor, 72. Synchronous pulley, 73. Idler pulley, 74. Belt, 75. Horizontal slide rail, 76. Connecting block, 77. Third slide block,

[0057] 8. First vertical moving mechanism, 81. First motor, 82. First screw rod, 83. First screw rod nut,

[0058] 9. Second vertical moving mechanism, 91. Second motor, 92. Second screw rod, 93. Second screw rod nut. DETAILED DESCRIPTION

[0059] The following will be combined with the accompanying drawings to clearly and completely describe the technical solutions in the utility model. Obviously, the described embodiments are part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0060] The "purification" of the present application is to extract or separate the target biomolecules by magnetic bead purification to obtain purified target biomolecules, and the purified target biomolecules include but are not limited to nucleic acids and proteins. Nucleic acid purification and protein purification have the same semantics as the common expressions "nucleic acid extraction" and "purified protein". In addition, the "purification" process may include the process of extracting from, for example, cell lysate and then purifying by magnetic bead adsorption, or it may be just the process of purifying by magnetic bead adsorption.

[0061] like Figure 1 As shown, the utility model provides a device for biological purification, including a circuit module 6, the circuit module has a PCB circuit board, and the PCB circuit board is provided with at least one motor power interface 63 and a socket strip interface 67. The working motors 61 are connected to the corresponding motor power interfaces 63 through their respective power lines 62, and all sensors 64 are connected to the socket strip interface 67 through their respective flat cables 65.

[0062] In one embodiment, the wire diameter of the power line 62 is larger than the wire diameter of the flat wire 65. The wire diameter of the power line 62 is 1 square millimeter, and the wire diameter of the flat wire 65 is 0.3 square millimeter. The power supply of the PCB circuit board to the working motor 61 is a 24 volt power supply, and the output current of the working motor 61 is 2.2A.

[0063] The output voltage and current of the working motor 61 when working are measured with an oscilloscope. The test results show that the current is consistent with the set value. The voltage power supply output is 24V, and the working motor input is 23.8V. The voltage from power supply output to motor input drops from 3V to 0.2V, meeting the actual use requirements.

[0064] like Figure 2 As shown, in a comparative embodiment, the working motor 61 is connected to the adapter terminal on the adapter board 66 through the respective power lines 62, and then connected to the power strip interface 67 through the flat line 65. The sensor 64 is connected to the adapter board 66 through the respective flat lines 65, and then connected to the same power strip interface 67 through the flat lines 65. The adapter board 66 and the power strip interface 67 are both on the PCB circuit board. Specifically, the wire diameter of the power line 62 is 1 square millimeter, and the wire diameter of the flat line 65 is 0.3 square millimeter.

[0065] When it comes to the porous reagent kit 0 with a separation layer, the comparative embodiment cannot meet the requirements due to the obvious voltage drop problem. The output voltage and current of the working motor when working were measured with an oscilloscope. The test results showed that the current was consistent with the set value. The voltage power supply end output 24V, and the working motor end input 21V, causing the power output of the working motor to drop by about 12.5% ​​compared with the set value, which cannot meet the actual purification needs. The most direct impact of the above-mentioned voltage drop is that the output force of the magnetic rod sleeve and the magnetic rod becomes smaller, resulting in the inability of the purification equipment to penetrate the separation layer in the porous reagent kit 0 under the design structure framework of the original equipment, making it impossible for the reaction liquids in the two reaction chambers to undergo a mixed reaction.

[0066] In one embodiment, the working motor 61 can drive the corresponding magnetic rod to be inserted into the corresponding magnetic rod sleeve, drive the magnetic rod and the magnetic rod sleeve to move downward to the specified position in the sleeve state, and move vertically back and forth according to the set cavity breaking force and cavity breaking number to break the separation layer in the porous reagent kit 0, so that the reaction liquid in the two reaction chambers separated by the separation layer is mixed. Specifically, the separation layer is made of a soft inert material that is not easy to react chemically with the chemical solution it contacts, so that it is convenient to be broken by subsequent operations, and under normal conditions, it is easy to be physically separated from water, such as different specific gravity, such as floating on water. Therefore, a paraffin layer is preferred, because the chemical properties of paraffin are very stable and it is not easy to react chemically with the separated reagent buffer; paraffin is lighter than water, and after being broken, it automatically floats up without affecting the components in the solution; and it is soft and can be easily broken to release the reaction reagents therein. When the separation layer is a paraffin layer, the paraffin fragments float directly on the liquid surface, which can not only allow the magnetic rod and the magnetic rod sleeve to smoothly pass through the separation position during the entire purification process and enter from one reaction chamber to another reaction chamber separated by the separation layer, but also avoid chemical or physical barriers to the purification process.

[0067] In one embodiment, Figure 3 As shown, the biological purification equipment also includes: a moving mechanism for driving the magnetic rod sleeve and / or the magnetic rod to move vertically and / or horizontally, further, the moving mechanism includes a horizontal moving mechanism and a vertical moving mechanism, further, the moving mechanism includes a horizontal moving mechanism 7 for driving the magnetic rod and the magnetic rod sleeve to move horizontally, and a first vertical moving mechanism 8 for driving the magnetic rod to move vertically respectively, and a second vertical moving mechanism 9 for driving the magnetic rod sleeve to move vertically. Specifically, the purification equipment adopts the same magnetic bead purification principle as the prior art, that is, the magnetic field around the magnetic rod sleeve is turned on and off by moving the magnetic rod up and down relative to the magnetic rod sleeve. When the magnetic rod is inside the magnetic rod sleeve, the magnetic beads are adsorbed to the surface of the magnetic rod sleeve and follow the magnetic rod sleeve to different slots of the multi-porous reagent kit 0; when the magnetic rod is at the upper part of the magnetic rod sleeve, the magnetic beads are separated from the magnetic rod sleeve and enter the reagent of the corresponding slot, and finally the purification of the target biological molecules is automatically realized.

[0068] In one embodiment, the first vertical moving mechanism 8 is a screw transmission structure, the second vertical moving mechanism 9 is a screw transmission structure, and the horizontal moving mechanism 7 is a pulley transmission structure. Specifically, the first vertical moving mechanism 8 includes: a first motor 81 (i.e., a working motor 61), a first screw 82, and a first screw nut 83. The first motor 81 is located at the bottom of the mounting frame, the output shaft of the first motor 81 is vertically upward, the first screw 82 is located on the output shaft of the first motor 81, the first screw nut 83 is sleeved on the first screw 82, and the first screw nut 83 is fixedly connected to the first slip ring. Specifically, when the first motor 81 is powered on, the first screw 82 rotates and drives the first screw nut 83 to move along the first screw 82, and at the same time, the first screw nut 83 links the first slider and the magnetic rod assembly 3 to move up and down along the vertical slide rail.

[0069] In the same embodiment, the second vertical moving mechanism 9 includes: a second motor 91 (i.e., the working motor 61), a second screw 92, and a second screw nut 93. The second motor 91 is located at the top of the mounting frame, the output shaft of the second motor 91 is vertically downward, the second screw 92 is located on the output shaft of the second motor, the second screw nut 93 is sleeved on the second screw 92, and the second screw nut 93 is fixedly connected to the second slip ring. Specifically, when the second motor 91 is powered on, the second screw 92 rotates and drives the second screw nut 93 to move along the second screw 92, and at the same time, the second screw nut 93 links the second slider and the magnetic rod sleeve assembly 4 to move up and down along the vertical slide rail.

[0070] In the same embodiment, the horizontal moving mechanism 7 includes: a synchronous pulley 72, an idler wheel 73, a belt 74, a third motor 71 (i.e., a working motor 61), and a horizontal slide rail 75. The belt 74 connects the synchronous pulley 72 and the idler wheel 73. The third motor 71 is rotatably connected to the synchronous pulley 72. A pair of third sliders 77 located at the bottom of the mounting frame 321 are respectively arranged on the two single rails of the horizontal slide rail 75. The third slider 77 is driven by the belt 74 to drive the mounting frame to move horizontally. Furthermore, the horizontal moving mechanism 7 also includes a connecting block 76. One end of the connecting block 76 is fixed on the belt 74, and the other end is fixedly connected to the mounting frame. Furthermore, the connecting block 76 is integrally formed with the mounting frame. Specifically, when the third motor 71 is turned, the synchronous pulley 72 rotates and drives the idler wheel 73 and the belt 74 to rotate together. At the same time, the belt 74 drives the connecting block 76 to move together, and the connecting block 76 is fixedly connected to the mounting frame on the top of the slider 77, so the belt 74 drives the mounting frame to move left and right along the horizontal slide rail 75, and accordingly, the magnetic bar and the magnetic bar sleeve arranged on the mounting frame also move left and right.

[0071] In one embodiment, Figure 4-5As shown, the biological purification device also includes: a frame 2, and a shell 1 surrounding the frame, wherein the frame 2 is a rectangular frame, the PCB circuit board is arranged at the rear part of the frame 2, and a pair of first extension parts 21 and second extension parts 22 extending forward are symmetrically fixed on the left and right frames of the front side of the frame 2 facing the user, respectively, the second extension part 22 is longer than the first extension part 21, and the second extension part 22 on the same frame is parallel to the first extension part 21 and is located below the first extension part 21;

[0072] The outer shell 1 includes an installation front shell 11, which has a first opening and a second opening parallel to each other for installing a display screen and a hatch. Both sides of the installation front shell 11 fit in with the first extension part 21 and the second extension part 22 and are obliquely installed on the frame 2, and the rear upper side of the installation front shell 11 fits in with the front upper end side of the frame 2.

[0073] Specifically, the rear upper side of the front shell 11 refers to the upper side of the rear of the front shell 11 facing away from the user, and the front upper end of the frame 2 refers to the uppermost frame of the front side of the frame 2. Figure 4-5 As shown, a pair of first extension parts 21 and second extension parts 22 of unequal lengths and parallel to each other extending forward are symmetrically fixed on the front side of the frame 2, and the two sides of the front shell 11 are fitted with the first extension parts 21 and the second extension parts 21 to be installed obliquely on the frame 2, and the upper rear side of the front shell 11 is fitted with the upper front end of the frame 2, and a first opening for installing a display screen and a second opening for installing a cabin door are provided in parallel above and below the front shell 11, so that the front shell 11 can be installed obliquely on the frame 2 as a whole. In this way, the structures of the front shell 11 and the frame 2 are simplified: the frame 2 is a rectangular frame, and can be mass-produced by simply setting the first extension part 21 and the second extension part 22. The entire rectangular panel of the front shell 11 can be tilted and fitted to the frame 2, and the display screen and the cabin door can be integrated into one mounting structure and tilted at the same time, which further simplifies the mounting structure.

[0074] In one embodiment, the bio-purification device further comprises any one or more of the following:

[0075] The magnetic rod assembly 3 includes a magnetic rod frame for installing the magnetic rod;

[0076] The magnetic rod sleeve assembly 4 includes a magnetic rod sleeve frame for installing the magnetic rod sleeve, the magnetic rod sleeve frame is located below the magnetic rod frame, and each magnetic rod can be correspondingly inserted into the corresponding magnetic rod sleeve;

[0077] A placement component 5 is used to place at least one multi-porous reagent kit 0. The placement component 5 is located below the magnetic rod sleeve rack, and each magnetic rod sleeve can be correspondingly inserted into a corresponding multi-porous reagent kit 0;

[0078] A studio, used for setting a magnetic bar assembly 3, a magnetic bar sleeve assembly 4 and a placement assembly 5;

[0079] A moving mechanism, used to drive the magnetic rod sleeve and / or the magnetic rod to move vertically and / or horizontally. Further, the moving mechanism includes a horizontal moving mechanism and a vertical moving mechanism. Further, the moving mechanism includes a horizontal moving mechanism 7 for driving the magnetic rod and the magnetic rod sleeve to move horizontally, a first vertical moving mechanism 8 for driving the magnetic rod to move vertically, and a second vertical moving mechanism 9 for driving the magnetic rod sleeve to move vertically.

[0080] A circuit module, including a circuit structure integrated board for controlling the purification equipment to realize the purification operation;

[0081] A heat dissipation device, used for dissipating heat from the studio;

[0082] Disinfection device, used to disinfect the studio.

[0083] In one embodiment, Figure 6-8 As shown, the placement assembly 5 further includes: a locking structure 51, the locking structure 51 includes: a positioning portion 511, a connecting rod 513, a rotation fixing portion 512 and a pushing portion 514, wherein:

[0084] One end of the connecting rod 513 is rotatably connected to the pushing portion 514, and the other end is rotatably connected to the rotating fixing portion 512;

[0085] The positioning portion 511 is used to be fixed on the base;

[0086] The rotating fixing portion 512 is sleeved on the positioning portion 511 and can be driven by the connecting rod 513 to rotate on the positioning portion 511;

[0087] The pushing portion 514 is used to push the connecting rod 513 to drive the rotating fixing portion 512:

[0088] When the pushing part 514 pushes the connecting rod 513, the connecting rod 513 drives the rotating fixing part 512 to rotate to a suitable position to separate from the porous reagent kit 0, thereby achieving the fixing and unlocking of the porous reagent kit 0;

[0089] When the pushing portion 514 drives the connecting rod 513 to return to its original position, the connecting rod 513 drives the rotating fixing portion 512 to rotate to a suitable position to abut against the multi-porous reagent kit 0 , thereby achieving locking and fixing of the multi-porous reagent kit 0 .

[0090] The base here refers to any plane for placing the porous reagent kit 0 during use, not limited to the base of the equipment, such as a desktop, a laboratory table, etc.; pushing refers to moving the pushing portion 54 forward compared to its starting position. At this time, the connecting rod 53 is also driven by the pushing portion 54 to deviate from its initial position, and resetting refers to the connecting rod 53 returning to its initial position driven by the backward movement of the pushing portion 54. The locking structure stabilizes and firmly positions the porous reagent kit 0 by rotating the fixing portion 52, and the porous reagent kit 0 will not be displaced or even lifted during the purification process. The porous reagent kit 0 is easy to put in and take out, and it is not necessary to use a lot of force to complete the removal, fixation and positioning of the porous reagent kit 0, which is convenient to use.

[0091] In one embodiment, Fig. 9 As shown, the placement assembly 5 also includes a limiter 52, which is used to fix and limit the porous reagent kit 0. The limiter 52 is also provided with at least one elastic clamping member installation groove near the side of the porous reagent kit 0 to install the elastic clamping member 521, and the elastic clamping member 521 is used to clamp the porous reagent kit 0. Preferably, the elastic clamping member 521 is a marble. Specifically, the limiter 52 is provided with an elastic clamping member 521 for clamping the porous reagent kit 0, which increases the friction force on the porous reagent kit 0 and prevents the porous reagent kit 0 from not being displaced or even being lifted during the purification process.

[0092] In one embodiment, the unilateral distance between the stopper 52 and the porous reagent box 0 is not greater than 0.7 mm. In another embodiment, the unilateral distance between the stopper 52 and the porous reagent box 0 is not greater than 0.5 mm. In another embodiment, the unilateral distance between the stopper 52 and the porous reagent box 0 is not greater than 0.2 mm. Specifically, the unilateral distance between the stopper 52 and the porous reagent box 0 is limited to an appropriate range, that is, taking into account the dimensional tolerance of the porous reagent box, the dimensional range of the gap between the stopper 52 and the porous reagent box 0 is limited, so that the elastic force released when the elastic clamp 521 is separated from the elastic clamp mounting groove is greater than the friction force generated by the porous reagent box 0 being driven, thereby preventing the porous reagent box 0 from not being displaced or even being lifted during the purification process.

[0093] The above description is only a preferred embodiment of the present invention and is not intended to limit the scope of protection of the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A device for biological purification, characterized in that include: The circuit module has a PCB circuit board, on which at least one motor power interface and a socket strip interface are arranged, the working motors are connected to the corresponding motor power interfaces through their respective power lines, and all sensors are connected to the socket strip interfaces through their respective cables.

2. The biological purification device according to claim 1, characterized in that The wire diameter of the power wire is larger than the wire diameter of the flat wire.

3. The biological purification device according to claim 1 or 2, characterized in that: The wire diameter of the power line is 1 square millimeter, and the wire diameter of the flat cable is 0.3 square millimeter.

4. The biological purification device according to claim 1, characterized in that The PCB circuit board supplies a 24V power supply to the working motor, and the output current of the working motor is 2.2A.

5. The biological purification device according to claim 1, characterized in that The working motor can drive the corresponding magnetic rod to be inserted into the corresponding magnetic rod sleeve, drive the magnetic rod and the magnetic rod sleeve to move downward to the specified position in the sleeve state, and move back and forth vertically according to the set cavity breaking force and cavity breaking times to break the separation layer in the porous reagent box, so that the reaction liquids in the two reaction chambers separated by the separation layer are mixed.

6. The biological purification device according to claim 5, characterized in that Also includes: A moving mechanism is used to drive the magnetic rod sleeve and / or the magnetic rod to move vertically and / or horizontally. Further, the moving mechanism includes a horizontal moving mechanism and a vertical moving mechanism. Further, the moving mechanism includes a horizontal moving mechanism that drives the magnetic rod and the magnetic rod sleeve to move horizontally and a first vertical moving mechanism that drives the magnetic rod to move vertically and a second vertical moving mechanism that drives the magnetic rod sleeve to move vertically.

7. The bio-purification device according to claim 6, characterized in that: The first vertical moving mechanism is a screw transmission structure, the second vertical moving mechanism is a screw transmission structure, and the horizontal moving mechanism is a pulley transmission structure.

8. The biological purification device according to claim 1, characterized in that Also includes: A frame, and a housing surrounding the frame, wherein The frame is a rectangular frame, the PCB circuit board is arranged at the rear part of the frame, and a pair of first extension parts and second extension parts extending forward are symmetrically fixed on the left and right frames of the front side of the frame facing the user, the second extension part is longer than the first extension part, and the second extension part on the same frame is parallel to the first extension part and is located below the first extension part; The outer shell includes a mounting front shell, which has a first opening and a second opening parallel to each other for mounting a display screen and a hatch, and both sides of the mounting front shell are fitted in contact with the first extension portion and the second extension portion and are obliquely mounted on the frame, and the upper rear side of the mounting front shell is fitted in contact with the upper front end side of the frame.

9. The biological purification device according to claim 1, characterized in that Also includes any one or more of the following: A magnetic bar assembly, including a magnetic bar holder for mounting the magnetic bar; The magnetic rod sleeve assembly comprises a magnetic rod sleeve frame for installing the magnetic rod sleeve, the magnetic rod sleeve frame is located below the magnetic rod frame, and each magnetic rod can enter the corresponding magnetic rod sleeve; A placement component, used for placing at least one multi-porous reagent box, wherein the placement component is located below the magnetic rod sleeve rack, and each of the magnetic rod sleeves can be correspondingly inserted into the corresponding multi-porous reagent box; A studio, used for arranging the magnetic bar assembly, the magnetic bar sleeve assembly and the placement assembly; A moving mechanism, used to drive the magnetic rod sleeve and / or the magnetic rod to move vertically and / or horizontally, further, the moving mechanism includes a horizontal moving mechanism and a vertical moving mechanism, further, the moving mechanism includes a horizontal moving mechanism that drives the magnetic rod and the magnetic rod sleeve to move horizontally and a first vertical moving mechanism that drives the magnetic rod to move vertically and a second vertical moving mechanism that drives the magnetic rod sleeve to move vertically; A circuit module, including a circuit structure integrated board for controlling the purification equipment to implement the purification operation; A heat dissipation device, used for dissipating heat from the working room; A disinfection device is used to disinfect the working room.

10. The biological purification device according to claim 9, characterized in that The placement assembly further includes a locking structure, which includes: a positioning portion, a connecting rod, a rotation fixing portion and a pushing portion, wherein: One end of the connecting rod is rotatably connected to the pushing part, and the other end is rotatably connected to the rotating fixing part; The positioning portion is used to be fixed on the base; The rotating fixing part is sleeved on the positioning part and can be driven by the connecting rod to rotate on the positioning part; The pushing part is used to push the connecting rod to drive the rotating fixing part: When the pushing part pushes the connecting rod, the connecting rod drives the rotating fixing part to rotate to a suitable position to separate from the porous reagent box. When the pushing part drives the connecting rod to reset, the connecting rod drives the rotating fixing part to rotate to a suitable position to abut against the multi-porous reagent box.

11. The biological purification device according to claim 9, characterized in that The placement assembly also includes a limiting member, which is used to fix and limit the porous reagent kit. The limiting member is also provided with at least one elastic clamping member installation groove on the side close to the porous reagent kit to install the elastic clamping member, and the elastic clamping member is used to clamp the porous reagent kit.

12. The bio-purification device according to claim 11, characterized in that: The elastic clamping member is a marble.

13. The biological purification device according to claim 11, characterized in that The single-side distance between the limiting member and the porous reagent box is no more than 0.7 mm.

14. The biological purification device according to claim 11, characterized in that The single-side distance between the limiting member and the porous reagent box is no more than 0.5 mm.

15. The biological purification device according to claim 11, characterized in that The single-side distance between the limiting member and the porous reagent box is no more than 0.2 mm.