Microfluidic system for generating and detecting hot-melt polymer microspheres

Abstract

The utility model discloses a microfluidic system for generating and detecting hot-melt polymer microspheres, which comprises a generating device and a detecting device, the generation device is provided with a micro-fluidic chip, a liquid supply device communicated with a dispersed phase liquid inlet and a continuous phase liquid inlet of the micro-fluidic chip through an infusion tube A, a collection container communicated with a microsphere outlet of the micro-fluidic chip through an infusion tube B, and a heating unit capable of heating and insulating the micro-fluidic chip, the infusion tube A and the liquid supply device. The detection device is provided with a microscope, and the microscope is arranged above the micro-fluidic chip so as to monitor the working state of the micro-fluidic chip. The micro-fluidic system is simple and reasonable in structure, ensures that the temperature of liquid in the micro-fluidic chip is not changed, and ensures the uniformity of the particle size of the generated microspheres; the microsphere generation efficiency is improved, the microsphere generation state of the micro-fluidic chip can be easily monitored in real time, and the production requirement is met.

Description

Technical Field

[0001] The utility model relates to the technical field of microfluidics, in particular to a microfluidics system for generating and detecting heat-soluble polymer microspheres. Background Art

[0002] At present, the application of hot-soluble gel microspheres (such as gelatin microspheres, methacrylated gelatin microspheres, agarose gel microspheres, etc.) in the field of biochemical applications and analysis is becoming more and more widespread. The current mainstream production methods include suspension polymerization, emulsion polymerization, membrane emulsification, seed method and microfluidics, among which microfluidics has attracted much attention because it can produce microspheres with more uniform particle size.

[0003] Since the state of the hot-soluble gel material will change differently with temperature, strict temperature control is required during the preparation of hot-soluble gel microspheres. The temperature control structure / method currently used in the industry is: placing the microfluidic chip in a constant temperature water bath to ensure the stability and consistency of the generation of hot-soluble gel microspheres. However, the above-mentioned temperature control structure has the following shortcomings: ① The above-mentioned temperature control structure only controls the temperature of the microfluidic chip (i.e., the "microsphere generation end"). In order to control and maintain the temperature of the liquid in the microfluidic chip to an appropriate node, it is required that the liquid flow rate is not too fast, which results in a low efficiency in microsphere generation and cannot meet production needs well. ② The above-mentioned temperature control structure is not conducive to the detection equipment to monitor the state of generated microspheres in the microfluidic chip.

[0004] In view of this, the present utility model is proposed. Summary of the invention

[0005] In order to overcome the above-mentioned defects, the utility model provides a microfluidic system for generating and detecting heat-soluble polymer microspheres. The system has a simple and reasonable structure, which not only well ensures that the temperature of the liquid in the microfluidic chip remains unchanged and ensures the uniformity of the particle size of the generated microspheres, but also greatly improves the efficiency of microsphere generation and facilitates real-time monitoring of the state of generated microspheres in the microfluidic chip, thus meeting production needs.

[0006] The technical solution adopted by the utility model to solve the technical problem is: a microfluidic system for generating and detecting heat-soluble polymer microspheres, comprising:

[0007] A generating device, which is provided with a microfluidic chip, a liquid supply device connected to the dispersed phase liquid inlet and the continuous phase liquid inlet of the microfluidic chip through a liquid infusion tube A, a collecting container connected to the microsphere outlet of the microfluidic chip through a liquid infusion tube B, and a heating unit capable of heating and keeping the microfluidic chip, the liquid infusion tube A and the liquid supply device warm;

[0008] The detection device is provided with a microscope; the microscope is arranged above the microfluidic chip to monitor the working state of the microfluidic chip.

[0009] As a further improvement of the present invention, the liquid supply device includes a first liquid storage container for supplying dispersed phase liquid, a second liquid storage container for supplying continuous phase liquid, and an air pressure pump. The first liquid storage container and the second liquid storage container are respectively connected to the dispersed phase liquid inlet and the continuous phase liquid inlet of the microfluidic chip through the infusion tube A, and the air pressure pump is respectively connected to the first liquid storage container and the second liquid storage container to pump out the liquid in the first liquid storage container and the second liquid storage container.

[0010] As a further improvement of the utility model, the heating unit includes a heating platform, a heating belt and a water bath device;

[0011] Correspondingly, the microfluidic chip is arranged on the heating table, the heating belt is wound around the outside of the infusion tube A, and the first liquid storage container and the second liquid storage container are both accommodated in the water bath equipment.

[0012] As a further improvement of the utility model, the heating platform adopts a constant temperature electric heating platform, and the water bath equipment adopts a constant temperature water bath pot.

[0013] As a further improvement of the utility model, a heat-conducting block is further arranged on the table top of the heating table, and the microfluidic chip is arranged on the heat-conducting block.

[0014] As a further improvement of the present invention, both the infusion tube A and the infusion tube B are made of Teflon tube.

[0015] As a further improvement of the present invention, the microfluidic chip comprises:

[0016] A chip substrate is disposed on the heating platform;

[0017] The liquid distribution channel is provided with a first distribution channel and a second distribution channel which are isolated from each other and are arranged on the chip substrate; the first distribution channel is provided with a plurality of first branch channels which are arranged at intervals and can be respectively communicated with the first liquid storage container, and the second distribution channel is provided with a plurality of second branch channels which are arranged at intervals and can be respectively communicated with the second liquid storage container;

[0018] A microsphere preparation structure, which is provided with a plurality of microsphere preparation modules also provided on the chip substrate; each of the microsphere preparation modules is provided with a first flow channel connected with the first branch flow channel through a first resistance flow channel, two second flow channels symmetrically connected at both sides of an output port of the first flow channel and respectively connected with the second branch flow channel through a second resistance flow channel, and a microsphere output flow channel respectively connected with the output ports of the first flow channel and the second flow channel, the first resistance flow channel being able to adjust the flow rate of the dispersed phase liquid flowing into the first flow channel, the second resistance flow channel being able to adjust the flow rate of the continuous phase liquid flowing into the second flow channel, so that the continuous phase liquid in the two second flow channels can symmetrically cut the dispersed phase liquid flowing out of the first flow channel to obtain microspheres, and the obtained microspheres are output via the microsphere output flow channel;

[0019] The converging channel is also arranged on the chip substrate and is selectively isolated from the first distribution channel and / or the second distribution channel; and the converging channel is provided with a plurality of third branch channels which are arranged at intervals and respectively connected to the microsphere output channel and the collection container.

[0020] As a further improvement of the utility model, the chip substrate comprises a first layer, a second layer and a third layer stacked in sequence;

[0021] The second distribution channel is disposed on any one of the first layer, the second layer and the third layer, and the microsphere preparation structure and the second distribution channel are located on the same layer;

[0022] The first distribution channel is also arranged on any one of the first layer, the second layer and the third layer. At the same time, the first distribution channel and the second distribution channel are not located on the same layer and are not arranged opposite to each other. In addition, the converging channel and the first distribution channel are located on the same layer.

[0023] As a further improvement of the present invention, the plurality of the first branch flow channels and the plurality of the third branch flow channels are all straight tube structures, and are arranged in parallel and alternately in sequence along the first direction;

[0024] The plurality of second branch flow channels are all U-shaped pipeline structures and are arranged side by side along a second direction perpendicular to the first direction; and a plurality of the microsphere preparation modules are arranged at intervals along the first direction between the two straight flow channel sections in each of the second branch flow channels;

[0025] In addition, the first distribution channel is further provided with a first main channel, and the first main channel can connect a plurality of the first branch channels to the infusion tube A corresponding to the first liquid storage container;

[0026] The second distribution channel is further provided with a second main channel, and the second main channel can connect a plurality of the second branch channels to the infusion tube A corresponding to the second liquid storage container;

[0027] The converging flow channel is also provided with a third main flow channel, and the third main flow channel can connect a plurality of the third branch flow channels to the infusion tube B.

[0028] As a further improvement of the present invention, the microfluidic system is further provided with an ultraviolet lamp, which is arranged above the outlet of the infusion tube B to irradiate and solidify the microspheres flowing out of the infusion tube B.

[0029] The beneficial effects of the present invention are as follows: compared with the prior art, the microfluidic system of the present invention has the following advantages: ① The microfluidic system is equipped with a heating unit capable of heating and keeping warm the microfluidic chip, the infusion tube A and the liquid supply device, that is, the present invention can heat and keep warm the "infusion end" and "microsphere generation end" of the system throughout the entire process, which not only well ensures that the temperature of the dispersed phase and continuous phase liquid in the microfluidic chip remains unchanged, and always maintains excellent hot soft glue fluidity, thereby well ensuring the uniformity of the particle size of the generated microspheres; and effectively avoids the constraints of temperature control on liquid flow rate in the prior art, thereby greatly improving the efficiency of microsphere generation and meeting production needs. ② The microscope provided in the microfluidic system is not affected by the heating unit, which is not only convenient and flexible to install, but also can monitor the state of generated microspheres of the microfluidic chip in real time. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 This is a schematic structural diagram of a microfluidic system for generating and detecting heat-soluble polymer microspheres according to the utility model;

[0031] Figure 2 This is a schematic diagram of the exploded structure of the microfluidic chip of the utility model;

[0032] Figure 3 This is a schematic structural diagram of the second distribution channel and the microsphere preparation structure of the utility model being arranged on the second layer of the chip substrate;

[0033] Figure 4 for Figure 3 The enlarged structural diagram of the A part is shown in FIG.

[0034] Figure 5 for Figure 4 Schematic diagram of the enlarged structure of the microsphere preparation module shown in;

[0035] Figure 6This is a schematic diagram of the structure in which the first distribution channel and the converging channel are arranged on the third layer of the chip substrate of the utility model.

[0036] Combined with the accompanying drawings, the following description is given:

[0037] 10. Microfluidic chip; 100. Chip substrate; 1001. First layer; 1002. Second layer; 1003. Third layer; 101. First distribution channel; 1010. First branch channel; 1011. First main channel; 102. Second distribution channel; 1020. Second branch channel; 10200. Direct flow channel; 1021. Second main channel; 103. Microsphere preparation module; 1030. First resistance channel; 1031. First channel; 1032. Second resistance channel channel; 1033, second channel; 1034, microsphere output channel; 1035, first through hole; 1036, second through hole; 104, converging channel; 1040, third branch channel; 1041, third main channel; 11, infusion tube A; 12, infusion tube B; 13, collecting container; 14, first liquid storage container; 15, second liquid storage container; 16, air pressure pump; 17, heating table; 18, heating belt; 19, water bath equipment; 110, trachea; 2, microscope; 3, ultraviolet lamp. DETAILED DESCRIPTION

[0038] The preferred embodiments of the present invention are described in detail below in conjunction with the accompanying drawings.

[0039] Example:

[0040] Please refer to the attached Figure 1 As shown, this embodiment provides a microfluidic system for generating and detecting heat-soluble polymer microspheres, which mainly includes a generating device and a detecting device, wherein the generating device is provided with a microfluidic chip 10, a liquid supply device connected with the dispersed phase liquid inlet and the continuous phase liquid inlet of the microfluidic chip 10 through an infusion tube A11, a collecting container 13 connected with the microsphere outlet of the microfluidic chip 10 through an infusion tube B12, and a heating unit capable of heating and keeping warm the microfluidic chip 10, the infusion tube A11 and the liquid supply device; the detecting device is provided with a microscope 2, which is arranged above the microfluidic chip 10 to monitor the working state of the microfluidic chip 10 (i.e., the state of generating microspheres).

[0041] As can be seen from the above, compared with the prior art, the microfluidic system provided in this embodiment has the following advantages: ① The microfluidic system described in this embodiment is equipped with a heating unit that can heat and keep warm the microfluidic chip, the infusion tube A and the liquid supply device, that is, this embodiment can heat and keep warm the "infusion end" and "microsphere generation end" of the system throughout the process, which not only well ensures that the temperature of the dispersed phase and continuous phase liquid in the microfluidic chip remains unchanged, and always maintains excellent hot soft glue fluidity, thereby well ensuring the uniformity of the particle size of the generated microspheres; and effectively avoids the constraints of temperature control on liquid flow rate in the prior art, thereby greatly improving the efficiency of microsphere generation and meeting production needs. ② The microscope equipped in the microfluidic system described in this embodiment is not affected by the heating unit, which is not only convenient and flexible to install, but also can monitor the state of generated microspheres of the microfluidic chip in real time.

[0042] The structure of the microfluidic system described in this embodiment is described in detail below.

[0043] First, regarding the liquid supply device and the heating unit.

[0044] Please continue to refer to the attached Figure 1 As shown, in this embodiment, the liquid supply device includes a first liquid storage container 14 for supplying dispersed phase liquid, a second liquid storage container 15 for supplying continuous phase liquid, and an air pressure pump 16, wherein the first liquid storage container 14 and the second liquid storage container 15 are respectively connected to the dispersed phase liquid inlet and the continuous phase liquid inlet of the microfluidic chip 10 through the infusion tube A11, and the air pressure pump 16 is respectively connected to the first liquid storage container 14 and the second liquid storage container 15 through the air pipe 110 to realize pumping out the liquid in the first liquid storage container 14 and the second liquid storage container 15. It can be understood that the air pressure pump 16 can provide independent air pressure kinetic energy for the first liquid storage container 14 and the second liquid storage container 15, so that the liquid in the first liquid storage container 14 and the second liquid storage container 15 flows out and flows into the dispersed phase liquid inlet and the continuous phase liquid inlet of the microfluidic chip 10 through the two infusion tubes A11.

[0045] Furthermore, the first liquid storage container 14 and the second liquid storage container 15 both adopt a tank structure.

[0046] Please continue to refer to the attached Figure 1 As shown, in this embodiment, the heating unit includes a heating platform 17, a heating belt 18 and a water bath device 19. Based on the above structure of the heating unit, the microfluidic chip 10 is arranged on the heating platform 17, the heating belt 18 is wound around the outside of the infusion tube A11, and the first liquid storage container 14 and the second liquid storage container 15 are both accommodated in the water bath device 19.

[0047] That is, in this embodiment, the microfluidic chip 10 is heated and kept warm by the heating platform 17, the infusion tube A11 is heated and kept warm by the heating belt 18, and the first liquid storage container 14 and the second liquid storage container 15 are heated and kept warm by the water bath device 19, which can well ensure that the dispersed phase and continuous phase liquid in the microfluidic chip remain unchanged and have good stability, thereby achieving good stability and uniformity in the microsphere generation process. In addition, heating and keeping warm the microfluidic chip 10 by the heating platform 17 is also very beneficial to the layout of the microscope 2, making it easy to install and layout.

[0048] It can be understood that in order to better heat and keep warm the microfluidic chip 10, the infusion tube A11 and the liquid supply device, in this embodiment, the heating platform 17 preferably adopts a constant temperature electric heating platform, the heating tape 18 preferably adopts a constant power electric heating tape, and the water bath equipment 19 preferably adopts a constant temperature water bath pot.

[0049] In addition, the structure of the present embodiment to realize that the microfluidic chip 10 is arranged on the heating platform 17 is as follows: a metal heat-conducting block is arranged on the surface of the heating platform 17, and the microfluidic chip 10 is arranged on the heat-conducting block. Based on the above-mentioned installation structure of the microfluidic chip 10, the microfluidic chip 10 is not only freely installed and not restricted by its own type, but also heated more evenly.

[0050] In addition, in this embodiment, the infusion tube A11 and the infusion tube B12 are both made of Teflon tube. Teflon tube has excellent chemical stability, aging resistance, electrical insulation, anti-stickiness, low friction coefficient, high transparency, easy observation and other characteristics; it can well meet production needs.

[0051] Next, the microfluidic chip 10 is described.

[0052] The microfluidic chip 10 described in this embodiment can adopt a variety of implementation structures, and the following is only an example for illustration: Figure 2 To Attachment Figure 6As shown, the microfluidic chip 10 includes a chip substrate 100, a liquid distribution channel, a microsphere preparation structure and a collection channel 104, wherein the chip substrate 100 is arranged on the heating platform 17 (specifically, it is arranged on the heat conductive block); the liquid distribution channel is provided with a first distribution channel 101 and a second distribution channel 102 which are isolated from each other and are arranged on the chip substrate 100, and the first distribution channel 101 is provided with a plurality of first branch channels which are arranged at intervals and can be connected to the first liquid storage container 14 respectively. 1010, the second distribution channel 102 is provided with a plurality of second branch channels 1020 which are arranged at intervals and can be respectively connected to the second liquid storage container 15; the microsphere preparation structure is provided with a plurality of microsphere preparation modules 103 which are also provided on the chip substrate 100, each of the microsphere preparation modules 103 is provided with a first channel 1031 which is connected to the first branch channel 1010 through a first resistance channel 1030, two second channels 1033 which are symmetrically connected to both sides of the output port of the first channel 1031 and are respectively connected to the second branch channel 1020 through a second resistance channel 1032, and a microsphere output channel 1034 which is respectively connected to the output ports of the first channel 1031 and the second channel 1033, the first resistance channel 1030 can adjust the flow rate of the dispersed phase liquid flowing into the first channel 1031, the second resistance channel 1032 can adjust the flow rate of the continuous phase liquid flowing into the second channel 1033, so as to achieve the two second channels 1033 having the same flow rate. The continuous phase liquid can symmetrically cut the dispersed phase liquid flowing out of the first channel 1031 to obtain microspheres, and the obtained microspheres are output through the microsphere output channel 1034; the converging channel 104 is also arranged on the chip substrate 100, and is selectively isolated from the first distribution channel 101 and / or the second distribution channel 102; and the converging channel 104 is provided with a plurality of third branch channels 1040 arranged at intervals and respectively connected to the microsphere output channel 1034 and the collection container 13.

[0053] It can be understood that the dispersed phase liquid in the first liquid storage container 14 flows through the infusion tube A11, the first distribution channel 101 (including the first branch channel 1010) and the first resistance channel 1030 in sequence, and then flows into the first channel 1031; the continuous phase liquid in the second liquid storage container 15 flows through another infusion tube A11, the second distribution channel 102 (including the second branch channel 1020) and the second resistance channel 1032 in sequence, and then flows into the second channel 1033; the continuous phase liquid in the two second channels 1033 efficiently and symmetrically cuts the dispersed phase liquid flowing out of the first channel 1031 to obtain a large number of microspheres with regular shapes and high monodispersity, and the obtained microspheres flow through the microsphere output channel 1034, the converging channel 104 (including the third branch channel 1040) and the infusion tube B12 in sequence, and then merge into the collecting container 13.

[0054] Furthermore, in this embodiment, the chip substrate 100 preferably adopts a three-layer plate structure, that is, please continue to refer to the attached Figure 2 As shown, the chip substrate 100 includes a first layer 1001, a second layer 1002 and a third layer 1003 stacked in sequence. Based on the three-layer plate structure of the chip substrate 100, ① the second distribution channel 102 can be arranged on any one of the first layer 1001, the second layer 1002 and the third layer 1003, and the microsphere preparation structure and the second distribution channel 102 are located on the same layer; Figure 2 1 shows the situation that the second distribution channel 102 and the microsphere preparation structure are both designed on the front of the second layer 1002; 2 the first distribution channel 101 can also be arranged on any one of the first layer 1001, the second layer 1002 and the third layer 1003, and the first distribution channel 101 and the second distribution channel 102 are not located on the same layer and are not arranged opposite to each other; in addition, the converging channel 104 and the first distribution channel 101 are located on the same layer; Figure 2 It shows a situation where both the first distribution channel 101 and the converging channel 104 are designed on the front side of the third layer 1003 .

[0055] This embodiment optimizes the layout of the liquid distribution channel, the microsphere preparation structure and the converging channel 104, so that the limited area of ​​the chip substrate can be fully utilized, thereby achieving the purpose of improving the chip structure integration.

[0056] Further, in this embodiment, the plurality of first branch flow channels 1010 and the plurality of third branch flow channels 1040 are all straight tube structures with one end closed, and are arranged in parallel and alternately in sequence along the first direction D1; Figure 2 , Attachment Figure 3 and attached Figure 6 The plurality of second branch flow channels 1020 are all U-shaped pipeline structures and are arranged side by side along a second direction D2 perpendicular to the first direction D1; and between the two straight flow sections 10200 in each of the second branch flow channels 1020, a plurality of the microsphere preparation modules 103 are arranged at intervals along the first direction D1; for details, please refer to the attached Figure 2 To Attachment Figure 4 As shown above, it can be seen that based on the first, second and third branch flow channels and the above layout of the microsphere preparation module, a large number of the microsphere preparation modules can be integrated on the limited area of ​​the chip substrate, thereby greatly improving the microsphere production speed in the field of microfluidic technology, that is, improving the production efficiency of the microfluidic chip, and well meeting the production needs of mass production of microspheres.

[0057] For further information, please refer to the attached Figure 2 and attached Figure 6 As shown, the first distribution channel 101 is also provided with a first main channel 1011, and the first main channel 1011 can connect the plurality of the first branch channels 1010 to an infusion tube A11 corresponding to the first liquid storage container 14; that is: while the first main channel 1011 is connected to the plurality of the first branch channels 1010, a port of the first main channel 1011 is also connected to an infusion tube A11 corresponding to the first liquid storage container 14. It can be understood that a port of the first main channel 1011 is used as the dispersed phase liquid inlet of the microfluidic chip 10.

[0058] Please continue to refer to the attached Figure 3 and attached Figure 4 As shown, the second distribution channel 102 is also provided with a second main channel 1021, and the second main channel 1021 can connect the plurality of second branch channels 1020 to an infusion tube A11 corresponding to the second liquid storage container 15; that is: while the second main channel 1021 is connected to the plurality of second branch channels 1020, a port of the second main channel 1021 is also connected to an infusion tube A11 corresponding to the second liquid storage container 15. It can be understood that a port of the second main channel 1021 is used as the continuous phase liquid inlet of the microfluidic chip 10.

[0059] Please continue to refer to the attached Figure 2 and attached Figure 6As shown, the converging channel 104 is also provided with a third main channel 1041, and the third main channel 1041 can connect several of the third branch channels 1040 to the infusion tube B12; that is: while the third main channel 1041 is connected with several of the third branch channels 1040, one port of the third main channel 1041 is also connected with the infusion tube B12. It can be understood that one port of the third main channel 1041 is used as the microsphere outlet of the microfluidic chip 10.

[0060] For further information, please refer to the attached Figure 5 As shown, in each of the microsphere preparation modules 103, the inner cross-sectional area of ​​the first resistance channel 1030 is much smaller than the inner cross-sectional area of ​​the first channel 1031, and the inner cross-sectional areas of the two second resistance channels 1032 are the same and much smaller than the inner cross-sectional area of ​​the second channel 1033. That is, in this embodiment, the first resistance channel 1030 and the second resistance channel 1032 are both formed by the circuitous micro-flow channel to ensure that the liquid transported through the first distribution channel 101 and the second distribution channel 102 is evenly distributed in different microsphere preparation modules 103, so as to complete the cutting action with high consistency and high symmetry, thereby greatly improving the monodispersity of microsphere preparation. In addition, by setting the first resistance channel 1030 and the second resistance channel 1032, the influence of the blockage of a single channel on the flow of other channels can also be reduced, thereby improving the service life and utilization rate of the chip.

[0061] In addition, in each of the microsphere preparation modules 103, the first flow channel 1031, the two second flow channels 1033 and the microsphere output flow channel 1034 together form a cross channel structure; the two second resistance flow channels 1032 are arranged in mirror symmetry. Moreover, in the plurality of microsphere preparation modules 103 connected to the same second branch flow channel 1020, each two adjacent microsphere preparation modules 103 are arranged in mirror symmetry, that is: each two adjacent microsphere preparation modules 103 share a first through hole 1035 for communicating with the first branch flow channel 1010, or share a second through hole 1036 for communicating with the third branch flow channel 1040; thereby, space can be well saved and the integrated layout of the product structure is facilitated.

[0062] Finally, about the other components.

[0063] Please continue to refer to the attached Figure 1 As shown, in this embodiment, the microfluidic system may also be provided with an ultraviolet lamp 3, which is arranged above the outlet of the infusion tube B12 to irradiate and solidify the microspheres flowing out of the infusion tube B12.

[0064] In addition, when the collection container 13 adopts an ice bath device, it can cool and solidify the microspheres, and the configuration of the ultraviolet lamp 3 can be omitted.

[0065] Finally, the prefixes "first", "second", etc. of the component names in this application specification (such as the first distribution channel, the second distribution channel, etc.) and the suffixes "A", "B", etc. of the component names (such as infusion tube A, infusion tube B, etc.) are only for the convenience of description and are not used to limit the scope of the implementation of this utility model patent.

[0066] In summary, compared with the prior art, the microfluidic system of the utility model has a simple and reasonable structure, which not only ensures that the liquid temperature in the microfluidic chip remains unchanged and ensures the uniformity of the particle size of the generated microspheres; it also greatly improves the microsphere generation efficiency and makes it easy to realize real-time monitoring of the generated microsphere state of the microfluidic chip, thus meeting production needs.

[0067] In the above description, many specific details are described to facilitate a full understanding of the present invention. However, the above description is only a preferred embodiment of the present invention. The present invention can be implemented in many other ways different from those described herein, so the present invention is not limited by the specific implementation disclosed above. At the same time, any person familiar with the technical field can make many possible changes and modifications to the technical solution of the present invention by using the methods and technical contents disclosed above without departing from the scope of the technical solution of the present invention, or modify it into an equivalent embodiment of equivalent changes. Any simple modification, equivalent change and modification made to the above embodiments based on the technical essence of the present invention without departing from the content of the technical solution of the present invention still falls within the scope of protection of the technical solution of the present invention.

Claims

1. A microfluidic system for generating and detecting thermosoluble polymer microspheres, characterized in that: include: A generating device, comprising a microfluidic chip (10), a liquid supply device connected to a dispersed phase liquid inlet and a continuous phase liquid inlet of the microfluidic chip (10) via a liquid infusion tube A (11), a collecting container (13) connected to a microsphere outlet of the microfluidic chip (10) via a liquid infusion tube B (12), and a heating unit capable of heating and keeping the microfluidic chip (10), the liquid infusion tube A (11) and the liquid supply device warm; A detection device is provided with a microscope (2); the microscope (2) is arranged above the microfluidic chip (10) to monitor the working state of the microfluidic chip (10).

2. The microfluidic system for generating and detecting heat-soluble polymer microspheres according to claim 1, characterized in that: The liquid supply device comprises a first liquid storage container (14) for supplying dispersed phase liquid, a second liquid storage container (15) for supplying continuous phase liquid, and an air pressure pump (16); the first liquid storage container (14) and the second liquid storage container (15) are respectively connected to the dispersed phase liquid inlet and the continuous phase liquid inlet of the microfluidic chip (10) through the infusion tube A (11); the air pressure pump (16) is respectively connected to the first liquid storage container (14) and the second liquid storage container (15) to pump out the liquid in the first liquid storage container (14) and the second liquid storage container (15).

3. The microfluidic system for generating and detecting heat-soluble polymer microspheres according to claim 2, characterized in that: The heating unit comprises a heating platform (17), a heating belt (18) and a water bath device (19); Correspondingly, the microfluidic chip (10) is arranged on the heating platform (17), the heating belt (18) is wound around the outside of the infusion tube A (11), and the first liquid storage container (14) and the second liquid storage container (15) are both accommodated in the water bath device (19).

4. The microfluidic system for generating and detecting heat-soluble polymer microspheres according to claim 3, characterized in that: The heating platform (17) is a constant temperature electric heating platform, and the water bath equipment (19) is a constant temperature water bath pot.

5. The microfluidic system for generating and detecting heat-soluble polymer microspheres according to claim 3, characterized in that: A heat-conducting block is also arranged on the table surface of the heating table (17), and the microfluidic chip (10) is arranged on the heat-conducting block.

6. The microfluidic system for generating and detecting heat-soluble polymer microspheres according to claim 1, characterized in that: The infusion tube A (11) and the infusion tube B (12) are both made of Teflon tubes.

7. The microfluidic system for generating and detecting heat-soluble polymer microspheres according to claim 3, characterized in that: The microfluidic chip (10) comprises: A chip substrate (100) is disposed on the heating platform (17); A liquid distribution channel, comprising a first distribution channel (101) and a second distribution channel (102) which are isolated from each other and arranged on the chip substrate (100); the first distribution channel (101) is provided with a plurality of first branch channels (1010) which are arranged at intervals and can be respectively communicated with the first liquid storage container (14); the second distribution channel (102) is provided with a plurality of second branch channels (1020) which are arranged at intervals and can be respectively communicated with the second liquid storage container (15); A microsphere preparation structure, comprising a plurality of microsphere preparation modules (103) also arranged on the chip substrate (100); each of the microsphere preparation modules (103) is provided with a first flow channel (1031) connected to the first branch flow channel (1010) through a first resistance flow channel (1030), two second flow channels (1033) symmetrically connected to both sides of an output port of the first flow channel (1031) and respectively connected to the second branch flow channel (1020) through second resistance flow channels (1032), and two second flow channels (1034) respectively connected to the first flow channel (1031) and the second flow channel (1010). The first resistance flow channel (1030) is capable of adjusting the flow rate of the dispersed phase liquid flowing into the first flow channel (1031), and the second resistance flow channel (1032) is capable of adjusting the flow rate of the continuous phase liquid flowing into the second flow channel (1033), so that the continuous phase liquid in the two second flow channels (1033) can symmetrically cut the dispersed phase liquid flowing out of the first flow channel (1031) to obtain microspheres, and the obtained microspheres are output through the microsphere output flow channel (1034); A converging flow channel (104) is also provided on the chip substrate (100) and is selectively isolated from the first distribution flow channel (101) and / or the second distribution flow channel (102); and the converging flow channel (104) is provided with a plurality of third branch flow channels (1040) which are arranged at intervals and are respectively connected to the microsphere output flow channel (1034) and the collection container (13).

8. The microfluidic system for generating and detecting heat-soluble polymer microspheres according to claim 7, characterized in that: The chip substrate (100) comprises a first layer (1001), a second layer (1002) and a third layer (1003) which are stacked in sequence; The second distribution channel (102) is disposed on any one of the first layer (1001), the second layer (1002) and the third layer (1003), and the microsphere preparation structure and the second distribution channel (102) are located on the same layer; The first distribution channel (101) is also arranged on any one of the first layer (1001), the second layer (1002) and the third layer (1003), and the first distribution channel (101) and the second distribution channel (102) are not located on the same layer and are not arranged opposite to each other; in addition, the converging channel (104) and the first distribution channel (101) are located on the same layer.

9. The microfluidic system for generating and detecting heat-soluble polymer microspheres according to claim 8, characterized in that: The plurality of first branch flow channels (1010) and the plurality of third branch flow channels (1040) are all straight tube structures, and are arranged in parallel and alternately in sequence along a first direction; The plurality of second branch flow channels (1020) are all U-shaped pipeline structures and are arranged side by side along a second direction perpendicular to the first direction; and a plurality of the microsphere preparation modules (103) are arranged at intervals along the first direction between the two straight flow channel sections (10200) in each of the second branch flow channels (1020); In addition, the first distribution channel (101) is further provided with a first main channel (1011), and the first main channel (1011) is capable of connecting a plurality of the first branch channels (1010) to a liquid infusion tube A (11) corresponding to the first liquid storage container (14); The second distribution channel (102) is further provided with a second main channel (1021), and the second main channel (1021) is capable of connecting a plurality of the second branch channels (1020) to a liquid infusion tube A (11) corresponding to the second liquid storage container (15); The converging flow channel (104) is further provided with a third main flow channel (1041), and the third main flow channel (1041) is capable of connecting a plurality of the third branch flow channels (1040) to the infusion tube B (12).

10. The microfluidic system for generating and detecting heat-soluble polymer microspheres according to claim 1, characterized in that: The microfluidic system is also provided with an ultraviolet lamp (3), which is arranged above the outlet of the infusion tube B (12) to irradiate and solidify the microspheres flowing out of the infusion tube B (12).

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