Rapid field detection device based on CRISPR-RPA-LFD technology

Abstract

The utility model relates to the technical field of biological detection, in particular to a rapid on-site detection device based on CRISPR-RPA-LFD (clustered regularly interspaced short palindromic repeats-recombinase polymerase amplification-lateral flow dipstick) technology, which comprises a connecting pipe, a limit strip arranged outside the connecting pipe, a first reaction component fixedly connected to the top inside the connecting pipe, a second reaction component fixedly connected to the inside of the connecting pipe and a buffer component fixedly connected to the inside of the connecting pipe. A flow guide assembly is fixedly connected to the bottom in the connecting pipe, and a test strip is arranged in the connecting pipe; the connecting pipe comprises a pipe body, a top cover is arranged on the top of the pipe body, and a first telescopic groove, a second telescopic groove and a third telescopic groove are formed in the pipe body. The reaction tube has the advantages that in the using process, as long as the limiting strip is not actively taken out of the first telescopic groove, the second telescopic groove and the third telescopic groove, the stability of the tube body can be ensured, and the situation that the reaction tube is punctured under the condition of misoperation or mistaken touch, so that an incompletely reacted reagent flows out of the punctured reaction tube is avoided; therefore, the detection accuracy is influenced.

Description

Technical Field

[0001] The utility model relates to the technical field of biological detection, in particular to a rapid on-site detection device based on CRISPR-RPA-LFD technology. Background Art

[0002] In recent years, with the development of society and the advancement of biomedical technology, the number of nucleic acid tests on various samples has increased significantly each year, and the requirements for on-site rapid detection and processing speed are becoming increasingly higher, and on-site operations require high sensitivity. The demand for simplicity and speed is also becoming increasingly urgent.

[0003] A closed experimental system device and application for rapid identification of nucleic acid amplification products disclosed in Chinese patent publication number CN105039149B includes a reaction tube, a sampling mixer and a reaction indicator box which are arranged in sequence from top to bottom, wherein the reaction tube has a sealable tube cover; the sampling mixer has a cutting object in the middle that can cut the reaction tube, the reaction indicator box has a sharp protrusion in the middle, and the reaction indicator box has a cavity at the tail end that can accommodate a lateral flow test strip.

[0004] However, compared with the prior art and the comparative scheme, it can be seen that the closed experimental system device and application for rapid identification of nucleic acid amplification products have the following problems during use:

[0005] When using the closed experimental system device and application for rapid identification of nucleic acid amplification products, the position of the reaction tube cannot be limited, and the sharp cutting object is located below the reaction tube. If the reaction tube is accidentally touched and moves downward during the mixing reaction, the sharp cutting object will cut the reaction tube, causing the incompletely reacted reagent to flow directly out of the cut reaction tube, thereby affecting the accuracy of the test result.

[0006] Therefore, this application proposes a rapid on-site detection device based on CRISPR-RPA-LFD technology to solve the above problems. Utility Model Content

[0007] The purpose of the present invention is to solve at least one of the above technical deficiencies.

[0008] The purpose of the utility model is to overcome the shortcomings of the prior art, solve the problems mentioned in the background technology, and provide a rapid on-site detection device based on CRISPR-RPA-LFD technology.

[0009] The purpose of the utility model is achieved through the following technical solutions: a rapid on-site detection device based on CRISPR-RPA-LFD technology, comprising a detection tube, a limiting strip is arranged on the outside of the detection tube, a first reaction component is fixedly connected to the top of the detection tube, a second reaction component and a buffer component are fixedly connected to the inside of the detection tube, a flow guide component is fixedly connected to the bottom of the detection tube, and a test strip is arranged inside the detection tube;

[0010] The detection tube comprises a tube body, a top cover is arranged on the top of the tube body, and a first telescopic groove, a second telescopic groove and a third telescopic groove are opened on the tube body;

[0011] The second reaction assembly includes a connecting tube, one end of which is fixedly connected to the first spike and the first flow guide cover, the connecting tube is fixedly connected to the first connecting rod, and the end of the connecting tube away from the first spike is fixedly connected to the second reaction tube;

[0012] The flow guide assembly comprises a flow guide pipe, one end of which is fixedly connected with a second spike and a second flow guide cover, and the flow guide pipe is fixedly connected with a second connecting rod.

[0013] Preferably, the first telescopic groove is located between the first reaction component and the second reaction component, the second telescopic groove is located between the second reaction component and the buffer component, and the third telescopic groove is located between the buffer component and the guide component. There are multiple limiting strips, which are respectively located inside the first telescopic groove, the second telescopic groove and the third telescopic groove.

[0014] Preferably, the first reaction assembly includes a first reaction tube, a top of the first reaction tube is fixedly connected with a connecting ring, and the first reaction tube is fixedly connected to the inside of the tube body through the connecting ring.

[0015] Preferably, the connecting pipe is fixedly connected to the inside of the pipe body through a first connecting rod.

[0016] Preferably, the structure of the buffer component is the same as that of the second reaction component.

[0017] Preferably, one end of the flow guiding tube away from the second spike is aligned with the test strip, and the flow guiding tube is fixedly connected to the inside of the tube body by a second connecting rod.

[0018] Compared with the existing technology, it has the following advantages:

[0019] During use, the rapid on-site detection device based on visual nucleic acid detection technology can ensure the stability of the tube body as long as the limiting strip is not actively removed from the first telescopic groove, the second telescopic groove and the third telescopic groove, thereby avoiding the reaction tube from being punctured due to operating errors or accidental touches, causing incompletely reacted reagents to flow out of the punctured reaction tube, resulting in a situation that affects the accuracy of the detection.

[0020] The parts not involved in the device are the same as those in the prior art or can be implemented by using the prior art. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is a cross-sectional structural schematic diagram of the utility model;

[0022] Figure 2 It is a schematic diagram of the structure of the utility model;

[0023] Figure 3 This is a schematic diagram of the structure of the detection tube of the utility model;

[0024] Figure 4 This is a schematic diagram of the structure of the first reaction component of the utility model;

[0025] Figure 5 This is a schematic diagram of the structure of the second reaction component of the utility model;

[0026] Figure 6 It is a structural schematic diagram of the flow guide component of the utility model.

[0027] In the figure: 1. detection tube; 101. tube body; 102. top cover; 103. first telescopic slot; 104. second telescopic slot; 105. third telescopic slot; 2. limiting strip; 3. first reaction component; 301. first reaction tube; 302. connecting ring; 4. second reaction component; 401. connecting tube; 402. first spike; 403. first flow guide cover; 404. first connecting rod; 405. second reaction tube; 5. buffer component; 6. flow guide component; 601. flow guide tube; 602. second spike; 603. second flow guide cover; 604. second connecting rod; 7. test strip. DETAILED DESCRIPTION

[0028] In the description of the present utility model, it should be understood that the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present utility model. In addition, the terms "first", "second", etc. are only used for descriptive purposes, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Therefore, features defined as "first", "second", etc. may explicitly or implicitly include one or more of the features. In the description of the present utility model, unless otherwise specified, "multiple" means two or more.

[0029] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installation", "connection" and "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be an indirect connection through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood by specific circumstances.

[0030] Additional aspects and advantages of the present invention will be further described below in conjunction with the accompanying drawings, and some will become apparent from the following description or be learned through practice of the present invention.

[0031] like Figure 1 and Figure 2 As shown, a rapid on-site detection device based on CRISPR-RPA-LFD technology includes a detection tube 1, the detection tube 1 is made of transparent material, a limiting strip 2 is arranged on the outside of the detection tube 1, a first reaction component 3 is fixedly connected to the top of the detection tube 1, a second reaction component 4 and a buffer component 5 are fixedly connected to the inside of the detection tube 1, a flow guide component 6 is fixedly connected to the bottom of the detection tube 1, and a test strip 7 is arranged inside the detection tube 1 (a connecting groove is arranged at the bottom of the detection tube 1, and the test strip 7 is placed inside the connecting groove);

[0032] like Figure 2 and Figure 3 As shown, the detection tube 1 includes a tube body 101, a top cover 102, a first telescopic groove 103, a second telescopic groove 104 and a third telescopic groove 105. The top cover 102 is arranged on the top of the tube body 101, and the first telescopic groove 103, the second telescopic groove 104 and the third telescopic groove 105 are opened on the tube body 101. The first telescopic groove 103 is located between the first reaction component 3 and the second reaction component 4, the second telescopic groove 104 is located between the second reaction component 4 and the buffer component 5, and the third telescopic groove 105 is located between the buffer component 5 and the guide component 6. There are multiple limiting strips 2, which are respectively located inside the first telescopic groove 103, the second telescopic groove 104 and the third telescopic groove 105;

[0033] like Figure 1 and Figure 4 As shown, the first reaction assembly 3 includes a first reaction tube 301 and a connecting ring 302. The top of the first reaction tube 301 is fixedly connected with the connecting ring 302. The first reaction tube 301 is fixedly connected to the inside of the tube body 101 through the connecting ring 302.

[0034] like Figure 1 and Figure 5As shown, the second reaction assembly 4 includes a connecting tube 401, a first spike 402, a first flow guide 403, a first connecting rod 404 and a second reaction tube 405, one end of the connecting tube 401 is fixedly connected to the first spike 402 and the first flow guide 403, the connecting tube 401 is fixedly connected to the first connecting rod 404, the connecting tube 401 is fixedly connected to the inside of the tube body 101 through the first connecting rod 404, and the end of the connecting tube 401 away from the first spike 402 is fixedly connected to the second reaction tube 405;

[0035] like Figure 1 As shown, the structure of the buffer component 5 is the same as that of the second reaction component 4;

[0036] like Figure 1 and Figure 6 As shown, the flow guide assembly 6 includes a flow guide tube 601, a second spike 602, a second flow guide cover 603 and a second connecting rod 604. The end of the flow guide tube 601 away from the second spike 602 is aligned with the test strip 7. One end of the flow guide tube 601 is fixedly connected to the second spike 602 and the second flow guide cover 603. The second connecting rod 604 is fixedly connected to the flow guide tube 601. The flow guide tube 601 is fixedly connected to the inside of the tube body 101 through the second connecting rod 604.

[0037] The working process is as follows:

[0038] S1. Before use, add the RPA amplification reaction reagent into the first reaction tube 301, add the CRISPR reaction reagent into the second reaction tube 405, and add the buffer solution into the reaction tube on the buffer assembly 5;

[0039] S2. When in use, add the sample to be detected into the first reaction tube 301, close the top cover 102, and react the sample to be detected with the RPA amplification reaction reagent in the first reaction tube 301;

[0040] S3. After the reaction is completed, the limiting strip 2 in the first telescopic groove 103 is removed, and then the two ends of the tube body 101 are pushed toward each other, the first telescopic groove 103 shrinks, the first spike 402 pierces the first reaction tube 301, and the reagents in the first reaction tube 301 that have completed the reaction flow into the second reaction tube 405 along the first flow guide 403 and the connecting tube 401, and react with the CRISPR reaction reagents in the second reaction tube 405;

[0041] S4, after the reaction is completed, the limiting strip 2 in the second telescopic groove 104 is removed, and then the two ends of the tube body 101 are pushed toward each other, the second telescopic groove 104 shrinks, and the spikes on the buffer assembly 5 pierce the second reaction tube 405, and the reagents in the second reaction tube 405 that have completed the reaction flow along the guide cover and the pipeline on the buffer assembly 5 into the reaction tube on the buffer assembly 5, so that the solution that has completed the reaction is mixed with the buffer solution in the reaction tube on the buffer assembly 5;

[0042] S5, then remove the limiting strip 2 in the third telescopic groove 105, push the two ends of the tube body 101 toward each other, so that the third telescopic groove 105 shrinks, and the second spike 602 punctures the reaction tube on the buffer assembly 5, and the mixed solution in the reaction tube on the buffer assembly 5 flows along the second flow guide cover 603 and the flow guide tube 601 to the test strip 7, and flows along the test strip 7 to the connecting groove inside the tube body 101, ensuring that the test strip 7 can absorb all the liquid;

[0043] S6. During use, as long as the limiting strip 2 is not actively removed from the first telescopic groove 103, the second telescopic groove 104 and the third telescopic groove 105, the stability of the tube body 101 can be ensured, avoiding the reaction tube from being punctured due to operational errors or accidental touches, causing the incompletely reacted reagents to flow out of the punctured reaction tube, resulting in a situation that affects the accuracy of the test. At the same time, it is convenient for rapid detection of samples in the field, preventing air from entering and causing pollution, and providing convenience for nucleic acid detection in the field.

[0044] The above shows and describes the basic principle, main features and advantages of the utility model. Those skilled in the art should understand that the utility model is not limited by the above embodiments, and the above embodiments and descriptions are only for explaining the principle of the utility model. Without departing from the spirit and scope of the utility model, the utility model may have various changes and improvements, and these changes and improvements fall within the scope of the utility model to be protected.

Claims

1. A rapid on-site detection device based on CRISPR-RPA-LFD technology, characterized in that: It comprises a detection tube, wherein a limiting strip is arranged on the outside of the detection tube, a first reaction component is fixedly connected to the top of the detection tube, a second reaction component and a buffer component are fixedly connected to the inside of the detection tube, a flow guide component is fixedly connected to the bottom of the detection tube, and a test strip is arranged inside the detection tube; The detection tube comprises a tube body, a top cover is arranged on the top of the tube body, and a first telescopic groove, a second telescopic groove and a third telescopic groove are opened on the tube body; The second reaction assembly comprises a connecting tube, one end of which is fixedly connected to a first spike and a first flow guide cover, a first connecting rod is fixedly connected to the connecting tube, and an end of the connecting tube away from the first spike is fixedly connected to a second reaction tube; The flow guide assembly comprises a flow guide pipe, one end of which is fixedly connected to a second spike and a second flow guide cover, and the flow guide pipe is fixedly connected to a second connecting rod.

2. A rapid on-site detection device based on CRISPR-RPA-LFD technology according to claim 1, characterized in that: The first telescopic groove is located between the first reaction component and the second reaction component, the second telescopic groove is located between the second reaction component and the buffer component, the third telescopic groove is located between the buffer component and the guide component, and there are multiple limiting strips, which are respectively located inside the first telescopic groove, the second telescopic groove and the third telescopic groove.

3. A rapid on-site detection device based on CRISPR-RPA-LFD technology according to claim 1, characterized in that: The first reaction assembly includes a first reaction tube. A connecting ring is fixedly connected to the top of the first reaction tube. The first reaction tube is fixedly connected to the inside of the tube body through the connecting ring.

4. A rapid on-site detection device based on CRISPR-RPA-LFD technology according to claim 1, characterized in that: The connecting pipe is fixedly connected to the inside of the pipe body through the first connecting rod.

5. A rapid on-site detection device based on CRISPR-RPA-LFD technology according to claim 1, characterized in that: The structure of the buffer component is the same as that of the second reaction component.

6. A rapid on-site detection device based on CRISPR-RPA-LFD technology according to claim 1, characterized in that: One end of the flow guiding tube away from the second spike is aligned with the test strip, and the flow guiding tube is fixedly connected to the inside of the tube body through the second connecting rod.

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