Anti-volatilization cell culture device

Abstract

The utility model discloses an anti-volatilization cell culture device which comprises a pore plate body and a plate cover, the pore plate body is provided with a containing groove, a plurality of culture tubes are arranged in the containing groove in an array mode, and liquid storage spaces are formed between the adjacent culture tubes and between the culture tubes and the containing groove. And the plate cover covers the pore plate body on one side of the accommodating groove. The device can effectively prevent the culture solution from volatilizing.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture, in particular to an anti-volatile cell culture device. Background Art

[0002] Cell culture vessels are commonly used devices for 2D cell adherent culture, cell suspension culture, and organoid 3D culture. During the culture process, the cell culture vessels need to be transferred under a microscope to observe the cell morphology in order to determine the progress of cell culture.

[0003] Existing cell culture vessels are generally porous structures, which can be 384-well, 96-well, 48-well or 12-well, specifically a multi-well plate, which has multiple culture wells arranged in an array. During the culture process, the solution in the culture wells will evaporate, especially the internal solution of the holes in the outermost circle or the outermost two circles of the multi-well plate. The solution evaporates too quickly, resulting in deviations in the culture results. Therefore, when using, it is customary to not use the outermost two circles of holes. This practice greatly reduces the number of cultures at one time, which is not conducive to use. Summary of the invention

[0004] The technical problem to be solved by the utility model is to provide an anti-volatile cell culture device which can effectively prevent the volatilization of culture solution.

[0005] In order to solve the above technical problems, the utility model provides an anti-volatile cell culture device, including a well plate body and a plate cover, the well plate body is provided with a receiving groove, a plurality of culture tubes are arrayed in the receiving groove, and a liquid storage space is formed between adjacent culture tubes and between the culture tubes and the receiving groove, and the plate cover is arranged on the well plate body on one side of the receiving groove.

[0006] Furthermore, a liquid absorbing sponge block is arranged in the liquid storage space.

[0007] Furthermore, an annular boss is provided on the inner surface of the cover plate corresponding to the culture tube.

[0008] Furthermore, a liquid injection hole is provided on the plate cover corresponding to the liquid storage space.

[0009] Furthermore, a rubber plug is provided on the injection hole.

[0010] Furthermore, a puncture portion is provided in the middle of the rubber plug.

[0011] Furthermore, an avoidance portion is provided on the side of the plate cover, and a gripping recess is provided on the orifice plate body corresponding to the avoidance portion.

[0012] Furthermore, a stepped edging is provided on the outer surface of the orifice plate body, the plate cover is provided on the small end of the stepped edging, and a limiting protrusion is provided on the top surface of the plate cover, and the limiting protrusion is used to limit the position of the stepped edging when the orifice plate body is stacked on the plate cover.

[0013] Furthermore, it also includes a mechanical clamp, which is provided with a placement groove, the orifice plate body is arranged in the placement groove, and a positioning pin and a positioning sleeve for matching positioning are arranged between the bottom of the orifice plate body and the bottom of the placement groove.

[0014] Furthermore, a magnet and stainless steel are provided between the bottom of the orifice plate body and the bottom of the placement slot for fixing in cooperation.

[0015] Beneficial effects of the utility model:

[0016] 1. In the well plate body, each hole for placing the culture solution is formed by an independent culture tube, and each culture tube exists independently, thereby forming a storage space for placing the solution. By placing the solution, the volatilization rate of the culture solution in the culture tube can be effectively reduced.

[0017] 2. The setting of the liquid-absorbing sponge block can absorb the solution in the liquid storage space, so that during the transfer process, the solution will not spill out due to shaking, and the solution in the liquid-absorbing sponge block can also be effectively retained and stored, providing a better culture environment. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0019] Figure 2 It is a schematic diagram of the structure of the orifice plate body of the utility model;

[0020] Figure 3 It is a schematic diagram of the plate cover structure of the utility model;

[0021] Figure 4 It is an exploded schematic diagram of the overall structure of the utility model with a liquid absorbing sponge block;

[0022] Figure 5 It is a schematic diagram of the structure of the plate cover with a liquid injection hole and a rubber plug of the utility model;

[0023] Figure 6 This is a schematic diagram of the utility model using a manually operated clamp in conjunction with the use;

[0024] Figure 7 This is a schematic diagram of the utility model using a mechanical operating fixture in conjunction with the use;

[0025] Figure 8 This utility model Figure 7 Schematic diagram of the cross section of the positioning structure;

[0026] Fig. 9 This utility model Figure 7 Schematic diagram of the fixed structure section;

[0027] Fig.10 It is a schematic structural diagram of an embodiment of the utility model in which a baffle is used instead of a moisture-absorbing sponge. DETAILED DESCRIPTION

[0028] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments so that those skilled in the art can better understand the present invention and implement it, but the embodiments are not intended to limit the present invention.

[0029] Reference Figures 1 to 5 As shown, an embodiment of the anti-volatile cell culture device of the utility model comprises a well plate body 1 and a plate cover 2, wherein a receiving groove 3 is provided on the well plate body, and a plurality of culture tubes 4 are arranged in an array in the receiving groove, and a liquid storage space is formed between adjacent culture tubes and between the culture tubes and the receiving groove, and the plate cover is provided on the well plate body on one side of the receiving groove. Water can be injected into the liquid storage space, and the humidity in the receiving groove of the well plate body can be maintained, so that the culture solution will not evaporate, thereby achieving an anti-volatile effect. Since water can flow in the liquid storage space, when the cell culture device is moved manually or by a manipulator, the water inside will sway around, even sway out, and may enter the culture tube, so a liquid absorbing sponge block 5 is provided in the liquid storage space, and the injected water will be absorbed by the liquid absorbing sponge block, and the water will not sway during the movement, which ensures the humidity in the receiving groove and is more convenient to use.

[0030] The above-mentioned culture tube and orifice plate body can be formed by integral injection molding, which is low in cost, stable in structure and accurate in alignment. The top of the culture tube is open and the bottom is a trough, forming a trough space for accommodating the culture solution. The culture tube can be round or square, preferably square, which can accommodate more culture solution. And an annular boss 6 is provided on the inner surface of the cover plate corresponding to the culture tube. The annular boss can form a relative sealing effect at the top opening of the culture tube while cooperating with the cover, providing good culture conditions.

[0031] Since the water injected into the liquid storage space will evaporate, in order to be able to replenish the liquid without opening the plate cover, an injection hole 7 is provided on the plate cover corresponding to the liquid storage space. There can be multiple injection holes, which are convenient for uniform water injection. The injection hole is an open hole. In order to prevent pollutants in the environment from entering the interior, a rubber plug 8 is provided on the injection hole for blocking, and a puncture portion is provided in the middle of the rubber plug, which can be a small hole. After the rubber plug made of rubber material is inserted into the injection hole, the small hole will be squeezed and closed, and cannot be connected to the outside. When the metal needle of the syringe is used to extend into the liquid storage space along the small hole, the rubber plug is deformed and avoided at the position of the small hole, and water can be smoothly injected. After the syringe is taken away, it can be closed again. Of course, the puncture portion can also be a thinner rubber layer, and the metal needle of the syringe can easily pierce it, which can also achieve the effect of isolating water injection.

[0032] In order to improve the storage effect of the cell culture device, a stepped edging 11 is provided on the outer surface of the well plate body, the plate cover is provided on the small end of the stepped edging, and a limiting protrusion 12 is provided on the top surface of the plate cover. The limiting protrusion is used to limit the position of the stepped edging when the well plate body is stacked on the plate cover. The multi-layer stacking can form a whole and will not slip.

[0033] Reference Figure 6 As shown, when the manual operation fixture 111 is used to cooperate with the microscope for observation, the orifice plate body located at the bottom of the plate cover will extend into the placement groove of the manual operation fixture, and at least two spring pieces on the manual operation fixture will squeeze the orifice plate body to the positioning position. Therefore, when the cell culture device is taken out manually, there is a pressing force, and the orifice plate body cannot be directly contacted, that is, the plate cover needs to be removed, and then the orifice plate body can be contacted and taken out, which will cause cross contamination and volatilization of the solution. Based on this, an avoidance portion 9 is provided on the side of the plate cover, through which the orifice plate body can be directly contacted, and the orifice plate body can be taken out without taking the plate cover. A grasping recess 10 is also provided on the orifice plate body corresponding to the avoidance portion, and the grasping recess can increase the contact area during manual grasping, and the fingertips enter the grasping recess to improve the grasping holding force. Of course, it can also cooperate with the manipulator to grasp and provide a grasping fulcrum.

[0034] Reference Figures 7 to 9 As shown, when a mechanical clamp 112 is used in conjunction with an inverted microscope for observation, a placement slot 13 is provided on the mechanical clamp, the well plate body is provided in the placement slot, and a positioning pin 14 and a positioning sleeve 15 for matching positioning are provided between the bottom of the well plate body and the bottom of the placement slot. After being grasped by the robot, it is placed in the mechanical clamp. By cooperating with at least two groups of positioning pins and positioning sleeves, the placement position can be consistent each time, thereby ensuring that the imaging position is consistent each time. If the imaging position is inconsistent each time, the growth curve of the organoid or cell will be inaccurate.

[0035] In order to further improve the placement accuracy, a magnet 16 and stainless steel 17 are arranged between the bottom of the orifice plate body and the bottom of the placement groove. During the placement of the orifice plate body, the positioning pin and the positioning sleeve cooperate to guide the movement, and finally the magnet and stainless steel are adsorbed to ensure that the orifice plate body cannot move. The magnetic suction method greatly improves the convenience of operation. There is no need to lock it. It can be taken away as long as the gripping force of the robot is greater than the magnetic suction force.

[0036] In one embodiment, referring to Fig.10 As shown, the moisture-absorbing sponge can also be replaced by a baffle 113. After the liquid is injected into the liquid storage space, the baffle can be placed on the surface of the liquid. The baffle is a transparent PC plate with a thickness of about 0.5mm to 1mm, which can prevent the liquid from shaking on the liquid surface.

[0037] After the baffle is set, it will cause obstruction to the replenishment of liquid. Therefore, an opening can be set on the surface of the baffle corresponding to the injection hole on the plate cover, so that the syringe used for injection can penetrate under the baffle, which is convenient and reliable to operate.

[0038] The above embodiments are only preferred embodiments for fully illustrating the present utility model, and the protection scope of the present utility model is not limited thereto. Equivalent substitutions or changes made by technicians in this technical field on the basis of the present utility model are all within the protection scope of the present utility model. The protection scope of the present utility model shall be subject to the claims.

Claims

1. A cell culture device for preventing volatility, characterized in that: It includes a well plate body and a plate cover. The well plate body is provided with a receiving groove. A plurality of culture tubes are arranged in an array in the receiving groove. Liquid storage spaces are formed between adjacent culture tubes and between the culture tubes and the receiving groove. The plate cover is arranged on the well plate body on one side of the receiving groove.

2. The anti-volatile cell culture device according to claim 1, characterized in that: A liquid absorbing sponge block or a baffle is arranged in the liquid storage space; the liquid absorbing sponge block is used to absorb the liquid in the liquid storage space; the baffle is arranged on the liquid surface in the liquid storage space.

3. The anti-volatile cell culture device according to claim 1, characterized in that: An annular boss is arranged on the inner surface of the cover plate corresponding to the culture tube.

4. The anti-volatile cell culture device according to claim 1, characterized in that: A liquid injection hole is arranged on the plate cover corresponding to the liquid storage space.

5. The anti-volatile cell culture device according to claim 4, characterized in that: The injection hole is provided with a rubber plug.

6. The anti-volatile cell culture device according to claim 5, characterized in that: A puncture portion is arranged in the middle of the rubber plug.

7. The anti-volatile cell culture device according to claim 1, characterized in that: An avoidance portion is arranged on the side of the plate cover, and a grabbing recess is arranged on the orifice plate body corresponding to the avoidance portion.

8. The anti-volatile cell culture device according to claim 1, characterized in that: A stepped edging is arranged on the outer surface of the orifice plate body, the plate cover is arranged on the small end of the stepped edging, and a limiting protrusion is arranged on the top surface of the plate cover, and the limiting protrusion is used to limit the position of the stepped edging when the orifice plate body is stacked on the plate cover.

9. The anti-volatile cell culture device according to claim 1, characterized in that: It also includes a mechanical clamp, which is provided with a placement groove, the orifice plate body is arranged in the placement groove, and a positioning pin and a positioning sleeve for matching positioning are arranged between the bottom of the orifice plate body and the bottom of the placement groove.

10. The anti-volatile cell culture device according to claim 9, characterized in that: A matched and fixed magnet and stainless iron are arranged between the bottom of the orifice plate body and the bottom of the placement slot.

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