Multi-immunochromatography detection chip

Abstract

The utility model relates to the technical field of immunodiagnosis, and particularly discloses a multiple immunochromatography detection chip which comprises a shell, three groups of detection mechanisms for detecting a sample solution are arranged in the shell, a main channel is formed in the shell, a fixed block is arranged on the main channel and divides the main channel into two sections, and the fixed block is arranged in the shell. A first channel and a second channel are formed in the fixed block, and a shunting mechanism for shunting a sample solution is arranged in the shell; the two detection mechanisms located on the two sides are connected with the two second channels correspondingly, and the two sides of the detection mechanism located in the middle are connected with the two main channels correspondingly. A sample adding cavity is formed in the shell, the sample adding cavity is communicated with the two groups of main channels, a movable block is arranged in the sample adding cavity, and the sample adding cavity is divided into two areas by the movable block; the technical problem of low detection efficiency caused by the defect that an existing detection card cannot simultaneously and rapidly detect a plurality of antigens or antibodies is solved.

Description

Technical Field

[0001] The utility model relates to the technical field of immune diagnosis, and specifically discloses a multiple immune chromatography detection chip. Background Art

[0002] Immunochromatography is widely used in clinical diagnosis, food safety, drug testing and other fields. It combines the analysis methods of immunoassay and chromatography to detect sample solutions through antigen-antibody binding reactions. Immunochromatography uses the capillary action of the microporous filter membrane to move the sample added to one end of the membrane strip to the other end. During the movement, the specific antigen or antibody in the sample solution binds to the antigen or antibody in the binding pad, so that it can be captured by the detection line, and the experimental results are determined by the color or luminescence of the marker.

[0003] Since antigens and antibodies affect each other, in the same sample solution, if the antigens or antibodies targeted by two sets of test lines have cross-reactions and thus affect each other, it is necessary to avoid placing the two test lines that will affect each other on the same nitrocellulose membrane; theoretically, the two ends of the same test line can react with their respective solutions independently and will not interfere with each other, but if the two solutions are mixed or cross-contaminated during the flow process, the signals detected at both ends of the same test line may be interfered; existing test cards have the disadvantage of being unable to simultaneously and quickly detect multiple antigens or antibodies under certain circumstances, resulting in low detection efficiency. Utility Model Content

[0004] In view of this, the purpose of the utility model is to provide a multiple immunochromatographic detection chip to solve the technical problem that the existing detection card cannot detect multiple target proteins simultaneously and quickly, resulting in low detection efficiency.

[0005] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a multiple immunochromatographic detection chip, comprising a shell, wherein three groups of detection mechanisms for detecting sample solutions are arranged in the shell, a main channel is provided in the shell, a fixed block is provided on the main channel, the fixed block divides the main channel into two sections, a first channel and a second channel are provided in the fixed block, and a shunting mechanism for shunting the sample solution is provided in the shell; two groups of the detection mechanisms located on both sides are respectively connected to the two groups of the second channels, and the two sides of the detection mechanism located in the middle are respectively connected to the two groups of the main channels; a sample loading cavity is provided in the shell, the sample loading cavity is composed of an ellipse and a circle with coincident centers, the sample loading cavity is connected to the two groups of the main channels, a movable block is provided in the sample loading cavity, the movable block divides the sample loading cavity into two areas, the shunting mechanism includes and a sliding block, and the movable block contacts the sliding block. The sample loading chamber is divided into two areas by a movable block, and with the help of a diversion mechanism, the same antigen or antibody can be detected on two groups of different sample solutions at the same time, or multiple antigens or antibodies can be detected on the sample solutions. The antigens or antibodies targeted by the two groups of detection lines have cross reactions, and multiple antigens or antibodies can be detected quickly, thereby improving the detection efficiency.

[0006] Furthermore, the shunt mechanism includes the sliding block, a sliding groove is provided in the shell, the sliding block is slidably mounted in the sliding groove, a spring is provided in the shell, one end of the spring contacts the sliding block, and the other end contacts the shell; a fixed groove is provided in the shell, the fixed groove is connected to the sliding groove, and two groups of control components for controlling the flow direction of the sample solution are provided in the fixed groove, and the two groups of control components are staggered up and down. According to specific circumstances, by adjusting the shunt mechanism, the flow direction of the sample solution is controlled, and the detection chip is adjusted to the state required by the staff, without the need for the staff to use multiple detection cards for detection, thereby improving the detection efficiency of the staff.

[0007] Furthermore, the control component includes a first rack, the first rack is fixedly connected to the sliding block, a gear is meshed on the first rack, the gear is rotatably connected to the housing, a second rack is meshed on the gear, the second rack is staggered and perpendicular to the first rack, a movable frame is slidably mounted in the fixed block, one end of the second rack is fixedly connected to the movable frame; a stopper is provided on the movable frame, and the stopper blocks the second channel. The control component can control the diversion mechanism. When using the control component, the staff only needs to rotate the movable block to control the diversion mechanism, which is simple to operate.

[0008] Furthermore, one group of the gears is connected to the housing via a first support base, and the other group of the gears is connected to the housing via a second support base, and a fixing platform is provided on the second support base. The two groups of gears are located at different heights through the fixing platform, so that the two groups of control components are located at different heights.

[0009] Furthermore, the housing includes an upper housing and a lower housing, and the upper housing is detachably connected to the lower housing; both sides of the upper housing and the lower housing are slidably mounted with card strips. The housing is detachable, and after use, the staff can disassemble the detection chip, clean and disinfect it, and continue to use it later, saving costs.

[0010] Furthermore, a sealing cover is provided on the upper shell, the sealing cover is fixedly connected in the sample loading chamber, and two groups of sample loading holes are provided on the sealing cover; a twist block is provided on the movable block, and the twist block is rotatably connected to the sealing cover. The movable block is controlled by the twist block, so as to achieve the purpose of controlling the diversion mechanism.

[0011] Furthermore, the detection mechanism includes a sample pad, a mixing pad, a nitrocellulose membrane and a water absorbent pad, wherein the sample pad is connected to one end of the mixing pad, the other end of the mixing pad is connected to one end of the nitrocellulose membrane, and the water absorbent pad is connected to the other end of the nitrocellulose membrane; and the nitrocellulose membrane is provided with several groups of detection lines and quality control lines. The detection mechanism can detect whether a sample solution contains a specific antigen or antibody.

[0012] Furthermore, the detection mechanism in the middle is divided into two parts by a partition block, and the partition block is fixedly connected to the lower shell. The partition block divides one group of detection mechanisms into two groups, so that two groups of sample solutions can be detected simultaneously.

[0013] The working principle and beneficial effects of this solution are:

[0014] When it is necessary to test the same antigen or antibody for two groups of different sample solutions, it is necessary to ensure that the movable block divides the sample loading chamber into two areas, and then the two groups of sample solutions are added to the sample loading chamber through the two groups of sample loading holes respectively, and the two groups of sample solutions flow to the two sides of the corresponding detection mechanism through the two groups of main channels and the two groups of first channels respectively, and the two groups of sample solutions are checked at the same time; when it is necessary to test multiple antigens or antibodies for the sample solution, and there is a cross reaction between the antigens or antibodies targeted by the two groups of detection lines, in order to ensure the accuracy of the test results, the control component drives the diversion mechanism to move by rotating the twist block, and the block is moved to the position where the first channel is blocked and the main channel is connected to the second channel; by controlling the diversion mechanism, two groups of different sample solutions can be tested at the same time or one group of sample solutions can be tested for multiple antigens or antibodies, and the staff does not need to add the sample solution to different detection cards for many times, which reduces the staff's operation time and improves the efficiency of the sample solution.

[0015] When the sample solution is tested by the detection mechanism, the sample solution is first absorbed by the sample pad, which adsorbs the interfering substances in the sample solution and adjusts its pH value. The sample solution then moves to the conjugate pad to label the designated antibody or antigen in the sample solution. The sample solution then moves from the conjugate pad to the nitrocellulose membrane, which captures the labeled antigen or antibody. The detection line adsorbs the labeled antigen or antibody. Finally, the sample solution moves to the absorbent pad, which is convenient for the staff to clean up later. The staff only needs to observe whether a color reaction occurs at the corresponding place of the detection line. The same antigen or antibody can be detected on two different sample solutions at the same time, making the detection efficiency higher.

[0016] Other advantages, objectives and features of the present invention will be described in the following description to some extent, and will be apparent to those skilled in the art based on the following examination and research, or can be taught from the practice of the present invention to some extent. The objectives and other advantages of the present invention can be achieved and obtained through the following description. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a structural schematic diagram of an embodiment;

[0018] Figure 2 An exploded view of an embodiment;

[0019] Figure 3 This is an enlarged schematic diagram of region A in the embodiment;

[0020] Figure 4 is a side sectional view of an embodiment;

[0021] Figure 5A schematic diagram of the structure of one form of the embodiment;

[0022] Figure 6 This is an enlarged schematic diagram of region B in the embodiment;

[0023] Figure 7 It is a structural schematic diagram of another form of the embodiment;

[0024] Figure 8 It is an enlarged schematic diagram of the C region in the embodiment.

[0025] The markings in the accompanying drawings are as follows: outer shell 1, upper shell 2, lower shell 3, card strip 4, sample loading chamber 5, sealing cover 6, sample loading hole 7, movable block 8, twist block 9, sample pad 10, mixing pad 11, nitrocellulose membrane 12, absorbent pad 13, detection line 14, quality control line 15, partition block 16, sliding block 17, sliding groove 18, spring 19, limit column 20, through groove 21, first rack 22 gear 23, second rack 24, movable frame 25, stop block 26, main channel 27, fixed block 28, first channel 29, second channel 30, first support seat 31, second support seat 32. DETAILED DESCRIPTION

[0026] The following is further described in detail through specific implementation methods:

[0027] Example

[0028] like Figures 1 to 8 As shown, a multiple immunochromatographic detection chip is disclosed, including a shell 1, the shell 1 includes an upper shell 2 and a lower shell 3, the upper shell 2 and the lower shell 3 are fixed by two sets of clamping strips 4, the two sets of clamping strips 4 are respectively located on both sides of the shell 1, two sets of limit blocks are arranged on the clamping strips 4, the upper shell 2 and the lower shell 3 are both provided with limit grooves, the two sets of limit blocks are respectively slidably clamped in the two sets of limit grooves; a sample adding cavity 5 is provided on the shell 1, the sample adding cavity 5 is composed of an ellipse and a circle, the center of the ellipse is aligned with the center of the circle The center of the circle of the shape coincides with that of the sample loading chamber 5, a sealing cover 6 is provided on the sample loading chamber 5, the shape of the sealing cover 6 is the same as that of the sample loading chamber 5, the sealing cover 6 is fixedly connected to the housing 1, two groups of sample loading holes 7 are opened on the sealing cover 6, and the two groups of sample loading holes 7 are respectively located on both sides of the sealing cover 6, a movable block 8 is provided in the sample loading chamber 5, the movable block 8 contacts the inner wall of the sample loading chamber 5, the movable block 8 separates the sample loading chamber 5 into two non-connected areas, a twisting block 9 is provided on the movable block 8, and the movable block 8 is rotatably connected to the sealing cover 6, such as Figure 1 and Figure 2 shown.

[0029] A diversion mechanism is provided in the housing 1, and the diversion mechanism includes a sliding block 17. A sliding groove 18 is provided in the housing 1. The sliding block 17 is slidably mounted in the sliding groove 18. A circular hole is provided in the housing 1, and the circular hole is connected to the sliding groove 18. A spring 19 is provided in the circular hole. One end of the spring 19 contacts the sliding block 17, and the other end contacts the bottom end of the circular hole. A limiting column 20 is provided on the upper housing 2. The limiting column 20 is installed in the spring 19, and one end of the limiting column 20 is installed on the sliding block 17. Figures 2 to 4 As shown; a fixed groove 33 is provided in the housing, and the fixed groove 33 is staggered with the sliding groove 18. Two groups of control components for controlling the flow direction of the sample solution are arranged in the fixed groove 33. The two groups of control components are respectively located on both sides of the sliding block 17. The two groups of control components are staggered up and down and are not located in the same horizontal plane; the control component includes a first rack 22, and a through groove 21 is provided on the sliding block 17. The first rack 22 is fixedly connected to the through groove 21, and the two ends of the first rack 22 are respectively in contact with the two side walls of the through groove 21. The first rack 22 is meshed with a gear 23, and the gear 23 is rotatably connected to the lower housing 3. The gear 23 is meshed with a second rack 24, and the second rack 24 is staggered with the first rack 22 up and down, which will not affect it. The first rack 22 is perpendicular to the second rack 24, the second rack 24 is fixedly connected to the movable frame 25, the movable frame 25 is slidably mounted in the fixed block 28, and a stopper 26 is provided on the movable frame 25; at this time, the sliding block 17 is completely located in the sliding groove 18, one end of the sliding block 17 is in contact with the movable block 8, and the spring 19 is in a compressed state; the first support seat 31 and the second support seat 32 are provided in the fixed groove 33, and a fixed platform is provided at the bottom end of the second support seat 32, and two sets of tooth grooves are respectively installed in the first support seat 31 and the second support seat 32, one set of gears 23 is in contact with the bottom surface of the fixed groove 33, and the other set of gears 23 is in contact with the top surface of the fixed platform, and the two sets of gears 23 are not located in the same horizontal plane, such as Figure 2 and Figure 3 shown.

[0030] Three groups of detection mechanisms are arranged in the housing 1. The detection mechanisms are card-mounted in the housing 1. The detection mechanisms include a sample pad 10, a mixing pad 11, a nitrocellulose membrane 12 and a water absorbent pad 13. One end of the sample pad 10 is placed above the mixing pad 11, one end of the mixing pad 11 is placed above the nitrocellulose membrane 12, the mixing pad 11 and the water absorbent pad 13 are respectively located at both ends of the nitrocellulose membrane 12, one end of the water absorbent pad 13 is located above the nitrocellulose membrane 12, and a plurality of groups of detection lines 14 and quality control lines 15 are arranged on the nitrocellulose membrane 12. The quality control lines 15 are close to the water absorbent pad 13, such as Figure 2As shown; the sample pad 10 is used to transport the sample solution while performing pretreatment, removing interfering substances in the sample solution, and adjusting its pH value, thereby improving the adsorption effect of the sample solution, thereby improving the sensitivity and accuracy of the detection; the mixing pad 11 contains specific reagents or materials for reacting with the target antigen or antibody, thereby separating or marking specific components in the sample solution, thereby improving the sensitivity and accuracy of the detection; the nitrocellulose membrane 12 is used to capture the antigen or antibody marked by the mixing pad 11, and cooperates with the detection line 14 to ensure that the sample solution contains the target antigen or antibody, and the quality control line 15 is used to judge the validity of the test paper; the absorbent pad 13 is used to absorb the sample solution, which is convenient for the staff to clean the sample solution; wherein the sample pad 10, the mixing pad 11, the nitrocellulose membrane 12 and the absorbent pad 13 are all technologies well known to those skilled in the art.

[0031] Two groups of main channels 27 are provided in the housing 1, and a fixing block 28 is located on the main channels 27. The fixing block 28 divides the main channels 27 into two sections. A first channel 29 and a second channel 30 are provided on the fixing block 28. The stopper 26 is L-shaped. The stopper 26 blocks the second channel 30. Both ends of the first channel 29 are respectively connected to the two groups of main channels 27, and the two groups of second channels 30 are respectively connected to two of the detection mechanisms; the remaining group of detection mechanisms are respectively connected to the two groups of main channels 27 on both sides, and a partition block 16 is provided on the lower housing 3, and one group of detection mechanisms is divided into two parts by the partition block 16; Figure 5 and Figure 6 shown.

[0032] Figure 5 and Figure 7 All of them are top views of the immunochromatographic detection chip after the upper shell 2 is removed.

[0033] When implementing it specifically,

[0034] During use, when it is necessary to test the same antigen or antibody for two groups of different sample solutions, the current state is maintained, and the two groups of sample solutions are added to the sample loading chamber 5 through the two groups of sample loading holes 7 respectively. At this time, the sample loading chamber 5 is divided into two areas by the movable block 8, and the two groups of sample solutions will not mix with each other. The two groups of sample solutions pass through the two groups of main channels 27 respectively, and flow to the two ends of one group of detection mechanisms through the first channel 29. The sample solution is first absorbed by the sample pad 10, and the sample pad 10 adsorbs the interfering substances in the sample solution and adjusts the pH value of the sample solution to a suitable value. The sample solution moves to the binding pad through capillary phenomenon, and the binding pad labels the designated antibody or antigen in the sample solution, while improving the sensitivity and accuracy of the antigen or antibody. The sample solution moves from the binding pad to the nitrocellulose membrane 12, and the nitrocellulose membrane 12 captures the labeled antigen or antibody. The detection line 14 adsorbs the labeled antigen or antibody and reacts with the labeled antigen or antibody, thereby generating a color reaction. Finally, the sample solution moves to the absorbent pad, which is convenient for the staff to clean up later. Figure 5 and Figure 6 As shown, the same antigen or antibody can be detected in two groups of different sample solutions at the same time, making the detection efficiency higher.

[0035] When it is necessary to test the sample solution for multiple antigens or antibodies, and there is a cross reaction between the antigens or antibodies targeted by the two groups of detection lines 14, the twist block 9 is rotated, and the twist block 9 rotates on the sealing cover 6, and the twist block 9 drives the movable block 8 to rotate in the sample loading chamber 5 until the sample loading chamber 5 is connected to form a whole; at the same time, the movable block 8 is separated from the sliding block 17, and the spring 19 is extended to drive the sliding block 17 to slide in the sliding groove 18. When the sliding block 17 slides, the gear 23 is driven to rotate through the first rack 22, and the gear 23 drives the second rack 24 to move horizontally, and the second rack 24 drives the movable frame 25 to move, and the movable frame 25 drives the block 26 to move, and the block 26 disconnects the connection between the main channel 27 and the first channel 29, and connects the main channel 27 with the second channel 30; only need to add the sample solution from the sample loading hole 7, the sample solution passes through the two groups of main channels 27 and the two groups of second channels 30, and moves to the two groups of detection mechanisms respectively, and the sample solution is tested, such as Figure 7 and Figure 8 As shown, it is possible to avoid dividing the sample solution into two parts for separate detection, thereby reducing the time of the sample solution and improving the efficiency of the sample solution.

[0036] When the immunochromatographic detection chip is tested, the two sets of card strips 4 are slid on the outer shell 1 until the card block is separated from the outer shell 1, the fixation between the upper shell 2 and the lower shell 3 is released, and then the upper shell 2 is separated from the lower shell 3, and the three sets of detection mechanisms are taken out, the unused detection mechanisms are saved, and the used detection mechanisms are tested for their results, and then the upper shell 2 and the lower shell 3 are cleaned, disinfected and sterilized, and the shells are reused; the three sets of detection mechanisms are installed on the lower shell 3, and then the upper shell 2 is installed on the upper shell 2, and then the upper shell 2 and the lower shell 3 are fixed by the two sets of card strips 4.

[0037] The above is only an embodiment of the utility model, and the common knowledge such as the known specific structure and characteristics in the scheme is not described in detail here. It should be pointed out that for those skilled in the art, several deformations and improvements can be made without departing from the structure of the utility model, which should also be regarded as the protection scope of the utility model, and these will not affect the effect of the implementation of the utility model and the practicality of the utility model.

Claims

1. A multiple immunochromatographic detection chip, characterized in that: The invention comprises a shell, wherein three groups of detection mechanisms for detecting a sample solution are arranged in the shell, a main channel is opened in the shell, a fixing block is arranged on the main channel, the fixing block divides the main channel into two sections, a first channel and a second channel are opened in the fixing block, and a diversion mechanism for diverting the sample solution is arranged in the shell; The two groups of detection mechanisms located on both sides are respectively connected to the two groups of the second channels, and the two sides of the detection mechanism located in the middle are respectively connected to the two groups of the main channels; A sample loading chamber is provided in the shell, the sample loading chamber is composed of an ellipse and a circle with overlapping centers, the sample loading chamber is communicated with the two groups of main channels, a movable block is provided in the sample loading chamber, the movable block divides the sample loading chamber into two areas, the flow diversion mechanism includes a sliding block, and the movable block contacts the sliding block.

2. A multiple immunochromatographic detection chip according to claim 1, characterized in that: The diversion mechanism includes the sliding block, a sliding groove is provided in the housing, the sliding block is slidably mounted in the sliding groove, a spring is provided in the housing, one end of the spring is in contact with the sliding block, and the other end is in contact with the housing; A fixed groove is provided in the shell, the fixed groove is communicated with the sliding groove, and two groups of control components for controlling the flow direction of the sample solution are arranged in the fixed groove, and the two groups of control components are staggered up and down.

3. A multiple immunochromatographic detection chip according to claim 2, characterized in that: The control assembly includes a first rack, the first rack is fixedly connected to the sliding block, a gear is meshed on the first rack, the gear is rotatably connected to the housing, a second rack is meshed on the gear, the second rack is staggered with the first rack up and down and perpendicular to each other, a movable frame is slidably mounted in the fixed block, and one end of the second rack is fixedly connected to the movable frame; A stopper is arranged on the movable frame, and the stopper blocks the second passage.

4. A multiple immunochromatographic detection chip according to claim 3, characterized in that: One group of the gears is connected to the housing via a first support seat, and the other group of the gears is connected to the housing via a second support seat, wherein a fixing platform is arranged on the second support seat.

5. A multiple immunochromatographic detection chip according to claim 4, characterized in that: The housing comprises an upper housing and a lower housing, wherein the upper housing is detachably connected to the lower housing; Both sides of the upper shell and the lower shell are slidably mounted with clamping strips.

6. A multiple immunochromatographic detection chip according to claim 5, characterized in that: The upper shell is provided with a sealing cover, the sealing cover is fixedly connected in the sample loading chamber, and the sealing cover is provided with two groups of sample loading holes; The movable block is provided with a twist block, and the twist block is rotatably connected to the sealing cover.

7. A multiple immunochromatographic detection chip according to claim 6, characterized in that: The detection mechanism comprises a sample pad, a mixing pad, a nitrocellulose membrane and a water absorbent pad, wherein the sample pad is connected to one end of the mixing pad, the other end of the mixing pad is connected to one end of the nitrocellulose membrane, and the water absorbent pad is connected to the other end of the nitrocellulose membrane; Several groups of detection lines and quality control lines are arranged on the nitrocellulose membrane.

8. A multiple immunochromatographic detection chip according to claim 7, characterized in that: The detection mechanism located in the middle is divided into two parts by a partition block, and the partition block is fixedly connected to the lower shell.

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