Cell slide imaging support
By designing a scaffold that allows cells to climb onto slides for direct microscopic imaging, the problem of time consumption and sample loss during the sealing process is solved, and stable and flexible microscopic imaging effects are achieved.
Patent Information
- Application Number
- CN202420907537.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-04-29
- Publication Date
- 2025-01-24
- Estimated Expiration
- 2034-04-29
AI Technical Summary
In the prior art, cell crawlers need to be sealed before microscopic imaging observation, which not only takes time, but also requires high operating technology, which can easily lead to cells falling off or introducing bubbles, and the cleaning process may lose samples.
A cell climbing imaging bracket is designed, which includes a bottom plate, a pressure ring and a fixed structure. It allows cells to slide directly for microscopic imaging observation, eliminating the sealing step and adding liquid through the vessel structure to achieve short-term activity. Cell imaging.
A micro -imaging can be performed without sealing, avoiding the loss of cell samples, and improving the stability and flexibility of the imaging. It is suitable for low and middle and high -end microscopic imaging.
Smart Images
Figure CN222394061U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of biological experimental equipment, in particular to a cell climbing slice imaging bracket. Background Art
[0002] Cell slides, also known as slides, refer to growing cells on a glass slide immersed in cell culture medium. The surface of the glass slide used to prepare cell slides has a permanent cationic charge, which can make the frozen tissue or cells adhere firmly to the slide through electrostatic action and not easily detach. It is mainly used in immunocytochemistry, in situ hybridization, immunofluorescence and other technologies, and is an essential step in the above technologies.
[0003] Another necessary step in immunocytochemistry, in situ hybridization, immunofluorescence and other techniques is microscopic imaging. That is, after the cell slide is prepared, according to different research purposes, after processes such as washing, fixation, membrane penetration, adding antibodies, blocking, staining, etc., the cells on the slide are observed by microscopic imaging.
[0004] For common inverted microscopes, the working distance of medium and high power objective lenses that are generally greater than ten times is mostly 0.17 mm. Because the objective lens is located at the bottom of the sample, the distance between it and the cell can only accommodate a cover glass, that is, the thickness of the cell slide. Therefore, after the cell slide is prepared and processed accordingly according to the research purpose, it is generally not directly used for microscopic observation. It is also necessary to seal the slide, that is, the slide is adhered to the cell side facing down, and is turned upside down on a slide with a sealing agent added, so that the two fit as closely as possible, and the cells are sandwiched between the two. Wait for the sealing agent to dry, and the slide is adhered to the slide. Since the thickness of the slide is generally around 1 mm, which is far beyond the working distance of some medium and high power objective lenses, for an inverted microscope, the sample needs to be placed face down on the slide, and the slide only acts as a carrier to facilitate the sample to be placed on the microscope imaging bracket for observation. Since the common slide size diameter is too small for most microscope sample brackets, if the slide is not sealed on the slide, it is impossible to perform microscopic imaging on the slide.
[0005] Therefore, although sealing is beneficial for the long-term preservation of samples, it is not a necessary step for microscopic imaging observation, and in many cases it only serves as a carrier. However, sealing is not only time-consuming, but also has certain technical requirements for the operator. For example, improper operation may cause cell detachment or introduce bubbles around the cells that affect microscopic imaging. In addition, cleaning before sealing can also damage samples for some experiments. For example, in apoptosis and necrosis experiments, apoptotic cells are often not firmly attached to the wall or even suspended, and this part of the sample will be lost after cleaning. Utility Model Content
[0006] The utility model aims to provide a cell climbing slice imaging bracket to solve the problems existing in the above-mentioned prior art, without the need for steps such as sealing and the like, and can avoid the loss of cell samples.
[0007] To achieve the above purpose, the utility model provides the following solutions:
[0008] The utility model provides a cell slide imaging bracket, comprising a bracket bottom plate, a pressure ring that can extend into the receiving groove and press on the cell slide, and a fixing structure that can lock the position of the pressure ring. The bracket bottom plate is provided with a receiving groove for placing the cell slide, and an observation hole is provided at the bottom of the receiving groove; a waterproof structure is fixedly arranged at the bottom of the pressure ring.
[0009] Preferably, the bracket base plate includes a reinforcing plate and a supporting plate fixedly arranged below the reinforcing plate, the reinforcing plate is provided with a first through hole, the supporting plate is provided with a second through hole coaxial with the first through hole, the diameter of the first through hole is larger than the diameter of the second through hole, the accommodating groove is formed between the supporting plate and the inner wall of the first through hole, and the second through hole forms the observation hole.
[0010] Preferably, the waterproof structure is a waterproof silicone ring.
[0011] Preferably, a mounting groove is provided at the bottom of the pressure ring, and the waterproof silicone ring is fixedly arranged in the mounting groove.
[0012] Preferably, the fixing structure comprises a plurality of spring sheets evenly distributed around the first through hole, and each of the spring sheets is rotatably connected to the reinforcing plate at one end, and the other end can be pressed tightly onto the pressing ring.
[0013] Preferably, the spring sheet includes a flat section and a curved section, a screw can pass through the flat section and be screwed into the reinforcing plate, the spring sheet can rotate around the screw, and the end of the curved section has a clamping portion, which can be clamped onto the pressure ring.
[0014] Preferably, the diameter of the first through hole is 28.1 mm, and the diameter of the second through hole is 16 mm.
[0015] Preferably, the reinforcing plate and the supporting plate are both 70 mm in length and 50 mm in width, the reinforcing plate is not less than 3 mm in thickness, and the supporting plate is not more than 0.2 mm in thickness.
[0016] Preferably, the reinforcing plate and the supporting plate are both made of high-hardness materials.
[0017] Preferably, the spring sheet is made of stainless steel.
[0018] Compared with the prior art, the utility model has achieved the following technical effects:
[0019] The cell slide imaging support provided by the utility model can directly use the cell slide for microscopic imaging observation from the cell culture state by arranging the support bottom plate, the pressure ring and the fixing structure of the pressure ring, thereby eliminating the steps of sealing and the like, and avoiding the loss of cell samples caused by the steps of fixation and rinsing before sealing and the like; at the same time, a vessel structure can be formed between the upper part of the cell slide and the pressure ring, and culture medium, various buffer solutions and other liquids can be directly added, so that the cells on the cell slide can be imaged and observed for a short time of living cells; super-resolution microscopic experiments based on single-molecule localization can be performed by adding fluorescent flashing buffer solution; and the size of the support bottom plate is suitable for most microscope sample clamps; the thickness of the bottom support plate is ultra-thin, which largely eliminates the obstruction of the support plate to the objective lens, so that the effective imaging area of the cell slide is expanded to the vicinity of the edge of the slide; the reinforcing plate with a hard structure and the high-elasticity spring sheet made of stainless steel ensure the stability of the fixation of the cell slide, which is beneficial to reducing the focal plane drift in the imaging experiment. BRIEF DESCRIPTION OF THE DRAWINGS
[0020] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.
[0021] Figure 1 This is a schematic diagram of the overall structure of the cell slide imaging support provided by the utility model;
[0022] Figure 2 An assembly diagram of the pressure ring and the waterproof silicone ring provided by the utility model;
[0023] Figure 3 A top view of the cell slide imaging support provided by the utility model;
[0024] Figure 4 A schematic diagram of the structure of the cell slide imaging support provided by the utility model when in use
[0025] In the figure: 1- bracket bottom plate; 11- reinforcement plate; 12- supporting plate; 2- pressure ring; 21- waterproof silicone ring; 3- cell climbing sheet; 4- spring sheet; 5- objective lens; 6- lens oil; 7- imaging buffer. DETAILED DESCRIPTION
[0026] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.
[0027] The utility model aims to provide a cell climbing slice imaging bracket to solve the problems existing in the prior art, and can avoid the loss of cell samples without the need for sealing steps.
[0028] In order to make the above-mentioned purposes, features and advantages of the present invention more obvious and easy to understand, the present invention is further described in detail below in conjunction with the accompanying drawings and specific implementation methods.
[0029] The utility model provides a cell climbing imaging bracket, such as Figures 1 to 4 As shown, in this embodiment, it includes a bracket base plate 1, a pressure ring 2 that can be extended into the receiving groove and pressed on the cell climbing sheet 3, and a fixing structure that can lock the position of the pressure ring 2. A receiving groove for placing the cell climbing sheet 3 is provided on the bracket base plate 1, and an observation hole is provided at the bottom of the receiving groove; a waterproof structure is fixedly provided at the bottom of the pressure ring 2.
[0030] The bracket base plate 1 includes a reinforcing plate 11 and a supporting plate 12 fixedly arranged under the reinforcing plate 11. The reinforcing plate 11 and the supporting plate 12 are tightly fitted and fixed by screws. A first through hole is opened on the reinforcing plate 11, and a second through hole coaxial with the first through hole is opened on the supporting plate 12. The diameter of the first through hole is larger than the diameter of the second through hole. A receiving groove for supporting the cell climbing sheet 3 and the pressure ring 2 is formed between the supporting plate 12 and the inner wall of the first through hole. The second through hole forms an observation hole, which is a field of view window for microscopic imaging of the cell climbing sheet 3.
[0031] The waterproof structure is a waterproof silicone ring 21.
[0032] A mounting groove is provided at the bottom of the pressure ring 2. The depth of the mounting groove is 1.5 mm, the outer diameter of the groove is 19 mm, the outer diameter of the waterproof silicone ring 21 is 19 mm, and the wire diameter is 1.5 mm. The waterproof silicone ring 21 can be completely embedded in the mounting groove.
[0033] The fixed structure includes a plurality of spring sheets 4 evenly distributed around the first through hole, preferably four spring sheets 4, one end of each spring sheet 4 is rotatably connected to the reinforcing plate 11, and the other end can be pressed against the pressure ring 2. Before placing the cell climbing sheet 3, the spring sheet 4 can rotate arbitrarily in the horizontal direction to ensure that when the pressure ring 2 is placed, the position of the pressure ring 2 is not blocked. The cell climbing sheet 3 is placed in the receiving groove and covered with the pressure ring 2. The pressure ring 2 is pressed by the spring sheets 4. Liquids such as buffer solution can be added to the space formed between the pressure ring 2 and the cell climbing sheet 3. Under the action of the waterproof silicone ring 21, liquid extravasation can be avoided.
[0034] The shape of the spring sheet 4 in the vertical direction can be divided into two sections, including a flat section and a semicircular curved section. The purpose of setting the flat section is to eliminate the obstacle of the gap between the pressure ring 2 and the bracket bottom plate 1 to the rotation and movement of the spring sheet 4, while being able to flexibly rotate and compress the pressure ring 2. A screw can pass through the flat section and be tightened in the reinforcing plate 11. A gasket is provided between the nut of the screw and the reinforcing plate 11. The spring sheet 4 can rotate around the screw. The end of the curved section has a clamping part, which can be clamped on the pressure ring 2. After the pressure ring 2 equipped with a waterproof silicone ring 21 is pressed on the cell climbing sheet 3, the four spring sheets 4 clamp and fix the pressure ring 2.
[0035] The diameter of the first through hole is 28.1 mm, and the diameter of the second through hole is 16 mm, so that the support plate 12 protrudes a ring in the first through hole, which can support the cell climbing sheet 3. The outer diameter of the pressure ring 2 is 28 mm, which is 0.1 mm smaller than the diameter of the first through hole, so as to facilitate the removal and placement of the pressure ring 2. The inner diameter of the pressure ring 2 is 16 mm, which is the same as the diameter of the second through hole, so as to facilitate the observation of the relative position of the objective lens 5 and the cell climbing sheet 3. The diameter of the cell climbing sheet 3 is 18 mm to 28 mm. Within this diameter range, the cell climbing sheet 3 can be placed in the receiving groove for use.
[0036] The length of the reinforcing plate 11 and the supporting plate 12 are both 70 mm, and the width is 50 mm. The bracket base plate 1 of this size is suitable for being placed on most microscope sample racks. The thickness of the reinforcing plate 11 is not less than 3 mm, preferably 3 mm, and the thickness of the supporting plate 12 is not more than 0.2 mm, preferably 0.2 mm. The thickness of the pressure ring 2 is the same as the thickness of the reinforcing plate 11, preferably 3 mm.
[0037] The reinforcing plate 11 and the supporting plate 12 are both made of high-hardness materials, including but not limited to aluminum, copper, synthetic resin, plastic, etc., preferably 5052 aluminum alloy. The reinforcing plate 11 and the supporting plate 12 are both treated to prevent reflection, including but not limited to black anodizing, pasting black light-absorbing materials, etc.
[0038] The spring sheet 4 is a highly elastic spring sheet 4 made of stainless steel.
[0039] Figure 4The schematic diagram of the structure of the cell climbing imaging support provided by the utility model when in use, the imaging buffer 7 is installed in the vessel structure formed between the pressure ring 2 and the cell climbing sheet 3, and the sealing performance of the vessel structure reaches more than 24 hours without leakage under the action of the waterproof silicone ring 21, and the objective lens 5 of the inverted microscope is below the cell climbing sheet 3, and the objective lens 5 and the cell climbing sheet 3 can be dripped with mirror oil 6, which is matched according to the actual situation of the objective lens 5 used, and is shown in this embodiment, but it is not necessarily used, because most of the medium and low magnification objective lenses 5 do not need to be used. The cell climbing imaging support provided by the utility model can easily realize a variety of low, medium and high-end microscopic imaging, such as bright field microscope, fluorescence microscope, confocal microscope, total internal reflection fluorescence microscope, single molecule localization super-resolution microscope, etc.
[0040] The present invention uses specific examples to illustrate the principle and implementation of the present invention. The above examples are only used to help understand the method and core idea of the present invention. At the same time, for those skilled in the art, according to the idea of the present invention, there will be changes in the specific implementation and application scope. In summary, the content of this specification should not be understood as limiting the present invention.
Claims
1. A cell slide imaging support, characterized in that: include: A support bottom plate, wherein a receiving groove capable of placing a cell slide is formed on the support bottom plate, and an observation hole is formed at the bottom of the receiving groove; A pressure ring capable of extending into the receiving groove and pressing on the cell climbing sheet, wherein a waterproof structure is fixedly arranged at the bottom of the pressure ring; And a fixing structure capable of locking the position of the pressure ring.
2. A cell-climbing imaging support according to claim 1, characterized in that: The bracket base plate includes a reinforcing plate and a supporting plate fixedly arranged below the reinforcing plate, the reinforcing plate is provided with a first through hole, the supporting plate is provided with a second through hole coaxial with the first through hole, the diameter of the first through hole is larger than the diameter of the second through hole, the accommodating groove is formed between the supporting plate and the inner wall of the first through hole, and the second through hole forms the observation hole.
3. A cell-climbing imaging support according to claim 1, characterized in that: The waterproof structure is a waterproof silicone ring.
4. A cell-climbing imaging support according to claim 3, characterized in that: A mounting groove is provided at the bottom of the pressure ring, and the waterproof silicone ring is fixedly arranged in the mounting groove.
5. A cell-climbing imaging support according to claim 2, characterized in that: The fixing structure comprises a plurality of spring sheets evenly distributed around the first through hole, one end of each of the spring sheets is rotatably connected to the reinforcing plate, and the other end can be pressed tightly on the pressing ring.
6. A cell-climbing imaging support according to claim 5, characterized in that: The spring sheet includes a flat section and a curved section. A screw can pass through the flat section and be screwed into the reinforcing plate. The spring sheet can rotate around the screw. The end of the curved section has a clamping portion, which can be clamped onto the pressure ring.
7. A cell-climbing imaging support according to claim 2, characterized in that: The diameter of the first through hole is 28.1 mm, and the diameter of the second through hole is 16 mm.
8. A cell-climbing imaging support according to claim 2, characterized in that: The reinforcing plate and the supporting plate are both 70 mm in length and 50 mm in width. The reinforcing plate is not less than 3 mm thick, and the supporting plate is not more than 0.2 mm thick.
9. A cell-climbing imaging support according to claim 2, characterized in that: The reinforcing plate and the supporting plate are both made of high-hardness materials.
10. The cell-climbing imaging support according to claim 5, characterized in that: The spring sheet is made of stainless steel.