Nucleic acid sample adding and extracting device

Abstract

The utility model discloses a nucleic acid sample adding and extracting device which comprises a case, the needle cylinder is driven to move in the case, and a suction head is assembled at the end part of the needle cylinder in an inserting manner; the transverse plate is arranged in the case in a sliding mode in the vertical direction, a plurality of U-shaped openings used for containing the suction heads are formed in the transverse plate, and the U-shaped openings and the suction heads are matched in a blocking and detaching mode; and the placing plate is movably arranged in the case, keeps synchronous movement with the transverse plate through a transmission assembly, and is provided with a clamping device, and the clamping device moves upwards along with the transverse plate and clamps the suction head. According to the nucleic acid sample adding and extracting device, through the structural design of the placing plate, when the needle cylinder moves upwards, the placing plate and the clamping device are driven to synchronously move upwards, the clamping device clamps the suction head, when the U-shaped opening stops the suction head from moving upwards, the auxiliary suction head is separated from the needle cylinder, then the clamping device still clamps the suction head to move downwards, and the suction head is kept in a vertical state; suction head deflection can be avoided.

Description

Technical Field

[0001] The utility model relates to the technical field of biological detection, in particular to a nucleic acid sampling and extraction device. Background Art

[0002] The nucleic acid sampling and extraction device is a device used to extract DNA or RNA from biological samples. It uses a pipette gun to repeatedly transfer, lyse, wash, elute and perform a series of operations on the reagent solution in the sample tube. The pipette tip needs to be replaced repeatedly to avoid contamination of the reagent solution.

[0003] In conjunction with publication number CN211848004U, publication date 2020-11-03, a nucleic acid extraction instrument is disclosed.

[0004] In the prior art including the above-mentioned patent, a second horizontal plate is provided above the front end of the material tray, and a plurality of U-shaped openings are provided at the rear end of the second horizontal plate. The pipette tip can be inserted into the U-shaped opening and the upper end surface of the pipette tip can abut against the lower side surface of the second horizontal plate. When the pipette tip mentioned in the above-mentioned technology is inserted into the U-shaped opening, the syringe rises so that the upper end surface of the pipette tip abuts against the lower side surface of the second horizontal plate. The syringe continues to rise so that the pipette tip can fall off the syringe. A slot for accommodating the pipette tip is provided on the reagent plate, and the pipette tip can fall into the slot, which is convenient for quick replacement of the pipette tip. However, when the pipette tip is blocked by the U-shaped opening and pulled off, the pipette tip may be deflected due to the obstruction of the U-shaped opening to the pipette tip. In the process of falling into the slot, the degree of deflection is further increased, and finally deviates from the plug-in position of the slot, which is not conducive to collecting the pipette tip. Utility Model Content

[0005] The utility model aims to provide a nucleic acid sampling and extraction device to solve the above problems.

[0006] In order to achieve the above-mentioned object, the utility model provides the following technical solutions: a nucleic acid sampling and extraction device, comprising a chassis;

[0007] The syringe is driven to move in the chassis, and a suction head is plugged and assembled at the end thereof;

[0008] A horizontal plate is slidably arranged inside the chassis in a vertical direction, and is provided with a plurality of U-shaped openings for accommodating suction heads, wherein the U-shaped openings form a blocking fit with the suction heads;

[0009] A placement plate is movably arranged in the chassis, and moves synchronously with the horizontal plate through a transmission component. A clamp is arranged on the placement plate, and the clamp moves up with the horizontal plate and clamps the suction head.

[0010] Preferably, the placement plate can extend out of the chassis in a horizontal direction, and a plurality of grooves corresponding to the suction heads are formed on the placement plate, and the clamp is circumferentially arranged at the edge of the groove.

[0011] Preferably, the transmission assembly comprises a first rack fixedly arranged on the transverse plate and slidably arranged on the inner wall of the chassis;

[0012] A second rack fixedly disposed on the placement plate and slidably assembled on the inner wall of the chassis;

[0013] The gear set arranged on the inner wall of the chassis comprises two mutually meshing pinions and a large gear, and the pinion and the large gear are respectively meshed with the first rack and the second rack for transmission.

[0014] Preferably, a stopper is provided in the U-shaped opening to abut against the upper port of the suction head.

[0015] Preferably, the stopper is rotatably arranged on the inner wall of the U-shaped opening, and the stopper comprises a circular abutting portion and an arc-shaped rubber strip enclosing an upper port of the suction head and abutting against the syringe plug port.

[0016] Preferably, the clamp is rotatably arranged on the placement plate, a first end of which is provided with an anti-sliding block to abut against the outer wall of the suction head, and a second end opposite thereto is provided with an oblique fork portion, the oblique fork portion is abutted against by the lower port of the suction head to flip over.

[0017] In the above technical scheme, the utility model provides a nucleic acid sampling and extraction device, which has the following beneficial effects: through the structural design of the placement plate, when the syringe moves upward, the placement plate and the clamp are driven to move upward synchronously, and the clamp clamps the pipette tip, and when the U-shaped opening blocks the pipette tip from moving up, the auxiliary pipette tip is separated from the syringe, and then the clamp still clamps the pipette tip and moves it downward, so that the pipette tip remains in a vertical state, which can prevent the pipette tip from being skewed. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in the present utility model. For ordinary technicians in this field, other drawings can also be obtained based on these drawings.

[0019] Figure 1 An overall three-dimensional schematic diagram provided for an embodiment of the utility model;

[0020] Figure 2 A schematic diagram of the transmission assembly structure provided by an embodiment of the utility model;

[0021] Figure 3 A schematic diagram of the U-shaped opening, syringe and suction head structure provided in an embodiment of the utility model;

[0022] Figure 4 A schematic diagram of a U-shaped opening and a stopper provided in an embodiment of the utility model;

[0023] Figure 5 The embodiment of the utility model provides Figure 3 A is an enlarged schematic diagram.

[0024] Description of reference numerals:

[0025] 1. Chassis; 2. Syringe; 21. Suction head; 3. Horizontal plate; 31. U-shaped opening; 32. First rack; 33. Gear set; 34. Stopper; 341. Resistance part; 342. Arc-shaped rubber strip; 4. Placement plate; 41. Second rack; 42. Clamp; 421. Bevel fork part; 422. Anti-sliding block; 43. Groove. DETAILED DESCRIPTION

[0026] In order to enable those skilled in the art to better understand the technical solution of the present invention, the present invention will be further described in detail below in conjunction with the accompanying drawings.

[0027] like Figure 1-5 As shown, a nucleic acid sampling and extraction device comprises a chassis 1;

[0028] The syringe 2 is driven to move in the housing 1, and a suction head 21 is plugged and assembled at the end thereof;

[0029] A horizontal plate 3 is vertically slidably disposed inside the chassis 1, and is provided with a plurality of U-shaped openings 31 for accommodating the suction heads 21. The U-shaped openings 31 and the suction heads 21 form a blocking fit.

[0030] The placement plate 4 is movably arranged in the chassis 1 , and moves synchronously with the transverse plate 3 through a transmission assembly. A clamp 42 is arranged on the placement plate 4 , and the clamp 42 moves upward with the transverse plate 3 and clamps the suction head 21 .

[0031] Specifically, the syringe 2 is driven by the driving mechanism to slide in the chassis 1 in the horizontal direction, and drives the suction head 21 to move in the vertical direction at the end of the sample tube. The above electronic components are common technical knowledge known to those skilled in the art and will not be described in detail here.

[0032] Further, such as Figure 2As shown, both upper and lower ends of the vertical sliding track of the horizontal plate 3 are provided with limit blocks. In the initial state, the horizontal plate 3 is located at the lower limit block. After the task of sucking liquid is completed, the syringe 2 moves horizontally to the opening of the U-shaped opening 31, drives the suction head 21 to enter the U-shaped opening 31 and cooperates with the blocking of the U-shaped opening 31, then the syringe 2 is driven to move upward, and the suction head 21 moves upward accordingly, and drives the horizontal plate 3 to move upward by blocking the U-shaped opening 31. After the horizontal plate 3 moves a certain distance, it is blocked by the upper limit block and stops, and the original position of the placement plate 4 is located directly below the horizontal plate 3, and When the suction head 21 is located in the U-shaped opening 31, the clamp 42 is located directly below the suction head 21. When the horizontal plate 3 moves upward, the placement plate 4 is driven by the transmission assembly, so that the placement plate 4 moves upward synchronously, and drives the clamp 42 to move upward and clamp the suction head 21. At this time, the syringe 2 is still moving upward, and the suction head 21 is blocked by the U-shaped opening 31 and is pulled out of the plug-in interface of the syringe 2. The horizontal plate 3 moves downward under the action of its own weight until it is blocked and stopped by the lower end limit block. The placement plate 4 moves downward synchronously through the transmission assembly, and drives the clamp 42 and the suction head 21 to move down to their original positions.

[0033] In the above technology, through the structural design of the placement plate 4, when the syringe 2 moves upward, it drives the placement plate 4 and the clamp 42 to move upward synchronously, and the clamp 42 clamps the suction head 21, and when the U-shaped opening 31 blocks the suction head 21 from moving upward, the auxiliary suction head 21 is separated from the syringe, and then the clamp 42 still clamps the suction head 21 and moves it downward, so that the suction head 21 remains in a vertical state, which can prevent the suction head 21 from being skewed.

[0034] As an embodiment further provided by the present invention, the placement plate 4 can extend out of the chassis 1 in the horizontal direction, and a plurality of grooves 43 corresponding to the suction heads 21 are formed on the placement plate 4 , and the clamps 42 are circumferentially arranged at the edges of the grooves 43 .

[0035] Specifically, a groove for the placement plate 4 to slide is provided on the outer shell of the chassis 1, and a plurality of grooves 43 are provided in an array on the placement plate 4, so that the placement plate 4 and the replaced suction head 21 can extend out of the chassis 1 and be replaced by the staff. The new suction head 21 corresponds to the position of the groove 43 and moves the suction head 21 downward along the axis of the groove 43, and fixes the suction head 21 by the clamp 42, and then slides the placement plate 4 back to its original position in the horizontal direction, driving the syringe 2 downward through the U-shaped opening 31, and the syringe 2 is plugged into the suction head 21. Since the friction force of the clamp 42 on the suction head 21 is less than the friction force when the syringe 2 and the suction head 21 are plugged, the syringe 2 separates the suction head 21 from the clamp 42 when moving upward, and then the syringe 2 drives the suction head 21 to move between the U-shaped opening 31 and the clamp 42, and then moves horizontally along the opening of the U-shaped opening 31 to continue to pump liquid.

[0036] As another embodiment further provided by the present invention, the transmission assembly includes a first rack 32 fixedly disposed on the horizontal plate 3 and slidably disposed on the inner wall of the chassis 1;

[0037] A second rack 41 fixedly disposed on the placement plate 4 and slidably assembled on the inner wall of the chassis 1;

[0038] The gear set 33 disposed on the inner wall of the chassis 1 includes two mutually meshing pinions and a large gear, and the pinion and the large gear are respectively meshed with the first rack 32 and the second rack 41 for transmission.

[0039] Specifically, when the cross plate 3 moves upward, it drives the first rack 32 to move upward, thereby driving the gear set 33 to rotate, and driving the second rack 41 to move upward synchronously through the gear set 33. Since there are two gears in the gear set 33, the moving directions of the first rack 32 and the second rack 41 are always the same, and the moving stroke ratio of the first rack 32 and the second rack 41 is equal to the gear ratio of the small gear and the large gear, so that the moving stroke of the second rack 41 is greater than that of the first rack 32. When the cross plate 3 moves upward, the placement plate 4 moves up a certain distance relative to the cross plate 3.

[0040] As another embodiment further provided by the present invention, the stopper 34 is rotatably disposed on the inner wall of the U-shaped opening 31 , and the stopper 34 includes a circular abutment portion 341 and an arc-shaped rubber strip 342 enclosing the upper end of the suction head 21 to abut against the insertion port of the syringe 2 .

[0041] Specifically, when the syringe 2 drives the suction head 21 to move along the opening of the U-shaped opening 31, the arcuate rubber strip 342 opens to allow the suction head 21 to enter the U-shaped opening 31, and then the suction head 21 pushes the abutment portion 341, causing the stopper 34 to rotate and drive the arcuate rubber strip 342 to approach each other to enclose the upper port of the suction head 21 and abut against the plug-in port of the syringe 2. The syringe 2 moves upward and drives the suction head 21 upward, and the arcuate rubber strip 342 is blocked by the upper port of the suction head 21, and the cross plate 3 is driven to move up to a certain distance and then stopped by the upper end limit block. The syringe 2 moves upward and detaches from the suction head 21, and the suction head 21 is clamped by the clamper 42 and its position remains unchanged. At this time, the arcuate rubber strip 342 still abuts against the plug-in port, and the plug-in port is scraped and cleaned when the syringe 2 moves upward.

[0042] As another embodiment further provided by the present invention, the clamper 42 is rotatably arranged on the placement plate 4, and a first end thereof is provided with an anti-sliding block 422 to abut against the outer wall of the suction head 21, and a second end opposite thereto is provided with an inclined fork portion 421, which is abutted against the lower end of the suction head 21 to flip over.

[0043] Specifically, a sponge absorbing head is provided at the end of the oblique fork portion 421. When the clamp 42 moves upward, the lower port of the suction head 21 abuts against the oblique fork portion 421, and the residual reagent liquid at the lower port is absorbed by the sponge absorbing head, while the oblique fork portion 421 rotates, driving the clamp 42 to flip as a whole, and driving the anti-sliding block 422 to abut against the outer wall of the suction head 21 and clamp it. The suction head 21 is fixed by the pressure and friction force of the anti-sliding block 422 on the outer wall. Since the pressure of multiple anti-sliding blocks 422 on the suction head 21 is directed to the axis of the suction head 21, the suction head 21 can be prevented from deflecting.

[0044] Working principle: Figure 2As shown, both upper and lower ends of the vertical sliding track of the horizontal plate 3 are provided with limit blocks. In the initial state of the horizontal plate 3, the horizontal plate 3 is located at the lower limit block. After the task of sucking liquid is completed, the syringe 2 moves horizontally to the opening of the U-shaped opening 31, drives the suction head 21 to enter the U-shaped opening 31 and blocks with the U-shaped opening 31. When the syringe 2 drives the suction head 21 to move along the opening of the U-shaped opening 31, the arc-shaped rubber strip 342 opens to allow the suction head 21 to enter the U-shaped opening 31, and then the suction head 21 pushes the abutment portion 341, so that the stopper 34 rotates, and drives the arc-shaped rubber strip 342 to approach each other to enclose the upper port of the suction head 21 and cooperate with the needle The plug interface of the syringe 2 conflicts with each other, and the upward movement of the syringe 2 drives the suction head 21 to move upward. The upper end of the suction head 21 blocks the arc-shaped rubber strip 342, and drives the cross plate 3 to move up to a certain distance, and then stops due to the upper limit block. The original position of the placement plate 4 is located directly below the cross plate 3, and when the suction head 21 is located in the U-shaped opening 31, the clamp 42 is located directly below the suction head 21. When the cross plate 3 moves upward, it drives the first rack 32 to move upward, thereby driving the gear set 33 to rotate, and the second rack 41 is driven to move upward synchronously through the gear set 33. Since there are two gears in the gear set 33, the movement of the first rack 32 and the second rack 41 The directions are always the same, and the travel ratio of the first rack 32 to the second rack 41 is equal to the ratio of the number of teeth of the small gear and the large gear, so that the travel of the second rack 41 is greater than that of the first rack 32. During the upward movement of the horizontal plate 3, the placement plate 4 moves up a certain distance relative to the horizontal plate 3, and drives the clamp 42 to move upward, the lower port of the suction head 21 abuts against the inclined fork 421, and the residual reagent liquid at the lower port is absorbed by the sponge suction head, while it abuts against the rotation of the inclined fork 421, driving the clamp 42 to flip over as a whole, and driving the anti-sliding block 422 to abut against the outer wall of the suction head 21 and clamp it, and the suction head 21 is fixed by the pressure and friction of the anti-sliding block 422 against the outer wall. The suction head 21 is prevented from deflecting because the pressure of the multiple anti-sliding blocks 422 on the suction head 21 is directed to the axis of the suction head 21. At this time, the syringe 2 is still moving up, the syringe 2 is separated from the suction head 21, and the suction head 21 is clamped by the clamp 42, and the position remains unchanged. At this time, the arc rubber strip 342 is still in contact with the plug-in interface, and the plug-in interface is scraped and cleaned when the syringe 2 moves up. The cross plate 3 then moves downward under the action of its own weight until it is stopped by the lower limit block. The placement plate 4 moves downward synchronously through the transmission assembly, and drives the clamp 42 and the suction head 21 to move down to their original position, so that the suction head 21 remains in a vertical state, which can prevent the suction head 21 from deflecting.

[0045] The above only describes some exemplary embodiments of the present invention by way of illustration. It is undoubted that those skilled in the art can modify the described embodiments in various ways without departing from the spirit and scope of the present invention. Therefore, the above drawings and descriptions are illustrative in nature and should not be construed as limiting the scope of protection of the claims of the present invention.

Claims

1. A nucleic acid sampling and extraction device, characterized in that: including a chassis (1); A syringe (2) is driven to move in the housing (1), and a suction head (21) is plugged and mounted on the end thereof; A horizontal plate (3) is slidably arranged inside the chassis (1) in a vertical direction, and is provided with a plurality of U-shaped openings (31) for accommodating suction heads (21), wherein the U-shaped openings (31) and the suction heads (21) form a blocking fit; A placement plate (4) is movably arranged in the chassis (1), and moves synchronously with the transverse plate (3) via a transmission assembly. A clamp (42) is arranged on the placement plate, and the clamp (42) moves upward along with the transverse plate (3) and clamps the suction head (21).

2. A nucleic acid sampling and extraction device according to claim 1, characterized in that: The placement plate (4) can extend out of the chassis (1) in a horizontal direction, and a plurality of grooves (43) corresponding to the suction heads (21) are provided on the placement plate (4), and the clamp (42) is circumferentially arranged at the edges of the grooves (43).

3. A nucleic acid sampling and extraction device according to claim 1, characterized in that: The transmission assembly comprises a first rack (32) fixedly mounted on the transverse plate (3) and slidably mounted on the inner wall of the chassis (1); A second rack (41) fixedly mounted on the placement plate (4) and slidably mounted on the inner wall of the chassis (1); The gear set (33) is arranged on the inner wall of the chassis (1), and comprises two mutually meshing pinions and a large gear, and the pinion and the large gear are respectively meshed with the first rack (32) and the second rack (41) for transmission.

4. A nucleic acid sampling and extraction device according to claim 1, characterized in that: A stopper (34) is provided in the U-shaped opening (31) to abut against the upper port of the suction head (21).

5. A nucleic acid sampling and extraction device according to claim 4, characterized in that: The stopper (34) is rotatably disposed on the inner wall of the U-shaped opening (31), and the stopper (34) comprises a circular abutment portion (341) and an arc-shaped rubber strip (342) enclosing the upper port of the suction head (21) and abutting against the insertion port of the syringe (2).

6. A nucleic acid sampling and extraction device according to claim 1, characterized in that: The clamp (42) is rotatably disposed on the placement plate (4), a first end of which is provided with an anti-sliding block (422) for contacting the outer wall of the suction head (21), and a second end opposite thereto is provided with an oblique fork portion (421), the oblique fork portion (421) being contacted by the lower end of the suction head (21) for flipping.

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