Bioreactor automatic feeding device capable of preventing external infection

Abstract

The utility model discloses an external infection prevention bioreactor automatic feeding device which comprises a base, a support frame is fixedly mounted at the upper end of the base, a control module is fixedly mounted at the upper end of the support frame, and a timer is fixedly mounted at the upper end of the support frame; the device further comprises a first electronic scale installed at the upper end of the base, the first electronic scale is connected with the upper surface of the base through a suction cup, a reaction tank is arranged at the upper end of the first electronic scale, and the reaction tank is connected with a material supplementing bottle through a material conveying pipe. The timer in the bioreactor automatic feeding device capable of preventing external infection sends a signal to the control module according to set time, so that the control module controls the servo motor to operate, and the output end of the servo motor drives the different-direction threaded rod to rotate, so that the threaded connector in threaded connection with the outer side moves in the opposite direction or in the reverse direction; and the material conveying pipe is clamped, so that the opening state of the material conveying pipe is controlled, and the automatic material supplementing effect is achieved.

Description

Technical Field

[0001] The utility model relates to the technical field of bioreactors, in particular to an automatic feeding device for bioreactors which can prevent external infection. Background Art

[0002] Although process intensification for continuous operation has been successfully applied in the chemical industry, the biopharmaceutical industry mainly uses batch feeding rather than continuous or perfusion methods to produce stable monoclonal antibodies from Chinese hamster ovary cells. At the same time, in terms of capacity, the fed-batch process has been confirmed to be able to scale from laboratory scale to commercial production scale up to 25,000L, while the perfusion process is usually run to 1000L;

[0003] With years of development, bioreactor systems have reached a high level of integration and automation, but the operation of the reactor is still at the stage of manual operation. Whether it is feeding or sampling, it is still highly dependent on manual operation and requires manual recording of data. This makes the scale of the laboratory completely positively correlated with the personnel cost, making it difficult to form economies of scale in the process of enterprise expansion. On the other hand, manual operation also has the possibility of misoperation. Although the company has formulated a series of operating procedures to prevent the possibility of misoperation, there are still various situations that lead to unconscious misoperation and cause certain losses. At the same time, since the experimental process and data are completely recorded manually by the operator, in the increasingly stringent compliance review environment, the hidden dangers brought by this problem are also increasing day by day;

[0004] Therefore, it is necessary to take practical and effective technical measures to improve the degree of automation of reactor-related operations, improve operational efficiency, and reduce operational errors caused by human factors.

[0005] In view of the above problems, it is urgent to carry out innovative design based on the structure of the original bioreactor automatic feeding device. Utility Model Content

[0006] The purpose of the utility model is to provide an automatic feeding device for a bioreactor that can prevent external infection, so as to solve the problem that the operation of the reactor proposed in the above background technology still remains in the stage of manual operation, whether it is feeding or sampling, it is still highly dependent on manual operation, and manual data recording is required, which makes the scale of the laboratory completely positively correlated with the personnel cost, and it is difficult to form economies of scale during the expansion of the enterprise.

[0007] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: an automatic feeding device for a bioreactor to prevent external infection, comprising a base, and a support frame is fixedly installed on the upper end of the base, a control module is fixedly installed on the upper end of the support frame, and a timer is fixedly installed on the upper end of the support frame;

[0008] It also includes: a first electronic scale is installed on the upper end of the base, and the first electronic scale is connected to the upper surface of the base through a suction cup, a reaction tank is arranged on the upper end of the first electronic scale, and the reaction tank is connected to a feeding bottle through a feeding pipe, and a clamping assembly is installed on the outer side of the feeding pipe, and the feeding pipe is clamped by the clamping assembly to achieve the effect of automatic feeding.

[0009] Preferably, a second electronic scale is installed on the upper end of the base, and the second electronic scale is connected to the upper surface of the base through a suction cup, and a monitoring component is installed on the outside of the second electronic scale, so as to grasp the changes of materials in real time through the monitoring component, thereby reducing the error between the replenishment amount and the planned replenishment amount.

[0010] Preferably, the clamping assembly includes a servo motor and a counter-rotating threaded rod, the servo motor is fixedly mounted on the bottom of the support frame, and the counter-rotating threaded rod is fixedly mounted on the output end of the servo motor, both ends of the counter-rotating threaded rod are threadedly connected with threaded connectors, and a clamping plate is fixedly mounted on the lower end of the threaded connector, and the clamping plates are distributed correspondingly front and back with respect to the midpoint of the feed pipe; this structure can control the opening state of the feed pipe through the clamping of the clamping plate.

[0011] Preferably, a sliding track is installed at the lower end of the support frame, and a limiting rod is nested inside the sliding track, and the limiting rod is fixedly installed on the upper end of the splint; this structure can limit the angle of the splint by sliding the limiting rod on the inner side of the sliding track.

[0012] Preferably, the monitoring component includes a first weighing module and a second weighing module, the first weighing module is installed on the outside of the first electronic scale, and the second weighing module is installed on the outside of the second electronic scale; in this structure, the first weighing module and the second weighing module can record the weight of the raw material in real time.

[0013] Preferably, the first weighing module and the second weighing module are both connected to the control module via a signal line.

[0014] Preferably, a pressure pump is fixedly installed on the outside of the feeding bottle; the pressure pump of this structure can control the speed of feeding.

[0015] Compared with the prior art, the utility model has the beneficial effect that the automatic feeding device for the bioreactor to prevent external infection is provided with:

[0016] 1. The structure of automatic feeding, the timer sends a signal to the control module according to the set time, so that the control module controls the servo motor to run, and the servo motor output end drives the counter-rotating threaded rod to rotate, so that the threaded connector connected with the outer thread moves toward or in the opposite direction, clamps the feeding pipe, thereby controlling the opening state of the feeding pipe and achieving the effect of automatic feeding;

[0017] Furthermore, when the clamping blocks move toward each other or in the opposite direction, the limiting rod will slide forward and backward along the inner side of the sliding track, thereby limiting the clamping plate to prevent it from rotating;

[0018] 2. The flow control structure sends instructions to the pressure pump through the control module, so that the pressure pump changes the pressure value inside the feeding bottle, thereby regulating the feeding speed of the feeding bottle;

[0019] 3. A structure for improving accuracy is provided by installing the first weighing module and the second weighing module on the outside of the first electronic scale and the second electronic scale, so that the material changes inside the reaction tank and the feeding bottle can be monitored in real time through the first weighing module and the second weighing module, and the monitored information is transmitted to the control module. After analysis, the control module adjusts the size and time of the feed amount, thereby reducing the error between the actual feeding amount and the planned feeding amount.

[0020] 4. Anti-pollution structure, both ends of the feed pipe are sealed and connected to the reaction tank and the feeding bottle to prevent the outside air and impurities from entering the reaction tank and causing pollution. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 This is a schematic diagram of the front view structure of the utility model;

[0022] Figure 2 This is a side view structural diagram of the support frame of the utility model;

[0023] Figure 3 For this utility model Figure 1 The enlarged structural diagram at A in the middle;

[0024] Figure 4 This is a schematic diagram of the front view structure of the first electronic scale of the utility model;

[0025] Figure 5 This is a schematic diagram of the front view structure of the feeding bottle of the utility model;

[0026] Figure 6 It is a schematic diagram of the working process of the utility model.

[0027] In the figure: 1. base; 2. support frame; 3. control module; 4. timer; 5. first electronic scale; 6. first weighing module; 7. suction cup; 8. reaction tank; 9. second electronic scale; 10. second weighing module; 11. feeding bottle; 12. pressure pump; 13. feed pipe; 14. servo motor; 15. different direction threaded rod; 16. threaded connector; 17. clamp; 18. sliding track; 19. limit rod. DETAILED DESCRIPTION

[0028] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0029] The utility model provides the following technical solutions:

[0030] Embodiment 1: Figure 1-Figure 3 , Figure 6 The technical solution shown is an automatic feeding device for a bioreactor to prevent external infection, which discloses: a base 1, and a support frame 2 is fixedly installed on the upper end of the base 1, a control module 3 is fixedly installed on the upper end of the support frame 2, and a timer 4 is fixedly installed on the upper end of the support frame 2;

[0031] It also includes: a first electronic scale 5 is installed on the upper end of the base 1, and the first electronic scale 5 is connected to the upper surface of the base 1 through a suction cup 7, a reaction tank 8 is arranged on the upper end of the first electronic scale 5, and the reaction tank 8 is connected to a feeding bottle 11 through a feeding pipe 13, and a clamping assembly is installed on the outer side of the feeding pipe 13, and the feeding pipe 13 is clamped by the clamping assembly to achieve the effect of automatic feeding;

[0032] The clamping assembly includes a servo motor 14 and a different direction threaded rod 15. The servo motor 14 is fixedly installed at the bottom of the support frame 2, and the different direction threaded rod 15 is fixedly installed at the output end of the servo motor 14. The two ends of the different direction threaded rod 15 are threadedly connected with threaded connectors 16, and the lower end of the threaded connector 16 is fixedly installed with a clamping plate 17, and the clamping plate 17 is distributed front and back correspondingly with respect to the midpoint of the feed pipe 13; a sliding track 18 is installed at the lower end of the support frame 2, and a limiting rod 19 is nested inside the sliding track 18, and the limiting rod 19 is fixedly installed at the upper end of the clamping plate 17;

[0033] This structure firstly compiles the set program into the control module 3, and then starts to operate the reaction tank 8 to react and process the raw materials to be processed. After a period of time, the timer 4 sends a signal to the control module 3 according to the set time, so that the control module 3 controls the servo motor 14 to operate. After the servo motor 14 operates, the output end of the servo motor 14 drives the counter-rotating threaded rod 15 to rotate. The rotation of the counter-rotating threaded rod 15 drives the threaded connector 16 with threads connected at both ends to move in the opposite direction, thereby driving the two groups of clamps 17 connected at the lower end to move in the opposite direction (the limit rod 19 slides on the inside of the sliding track 18 to limit the angle of the clamp 17). After the two groups of clamps 17 lose the clamping of the feed pipe 13 in the middle, the feed pipe 13 will be in an open state, so that the supplementary raw materials inside the replenishment bottle 11 are transported to the reaction tank 8 under the action of the pressure pump 12 to replenish the raw materials.

[0034] Embodiment 2:

[0035] The difference from the first embodiment is that a structure for increasing the feeding accuracy is added on the basis of automatic feeding, such as Figure 1 , Figure 4 , Figure 6 The technical solution shown in the figure, the present invention provides a technical solution: an automatic feeding device for a bioreactor to prevent external infection, discloses:

[0036] A second electronic scale 9 is installed on the upper end of the base 1, and the second electronic scale 9 is connected to the upper surface of the base 1 through a suction cup 7. A monitoring component is installed on the outer side of the second electronic scale 9, and the change of the material can be grasped in real time through the monitoring component, so as to reduce the error between the feeding amount and the planned feeding amount; the monitoring component includes a first weighing module 6 and a second weighing module 10, the first weighing module 6 is installed on the outer side of the first electronic scale 5, and the second weighing module 10 is installed on the outer side of the second electronic scale 9; the first weighing module 6 and the second weighing module 10 are connected to the control module 3 through a signal line;

[0037] This structure places a first electronic scale 5 and a second electronic scale 9 at the lower ends of the reaction tank 8 and the feeding bottle 11 respectively, and weighs the weight of the reaction tank 8 and the feeding bottle 11 by the first electronic scale 5 and the second electronic scale 9. The weighed weight will be recorded by the first weighing module 6 and the second weighing module 10, and transmitted to the control module 3 in real time. After system analysis, the control module 3 adjusts the size and time of the feeding amount, thereby reducing the error between the actual feeding amount and the planned feeding amount and improving the feeding accuracy.

[0038] Embodiment three:

[0039] The difference from the first embodiment is that a structure for controlling the feeding speed is added on the basis of automatic feeding. Figure 1 , Figure 5-Figure 6 The technical solution shown in the figure, the present invention provides a technical solution: an automatic feeding device for a bioreactor to prevent external infection, discloses: a pressure pump 12 is fixedly installed on the outside of a feeding bottle 11;

[0040] This structure controls the pressure value inside the feeding bottle 11 through the pressure pump 12, and controls the feeding speed by controlling the pressure value.

[0041] Workflow:

[0042] Connect the bioreactor 8 with the control system of the reactor 8, place the bioreactor 8 and the feeding bottle 11 on the corresponding electronic scales respectively, and after completion, connect the relevant feeding bottle 11 to the reactor 8 through the feeding pipe 13, place the clamping assembly on the feeding pipe 13 away from the inlet end of the reactor 8, the pressure pump 12 and the servo motor 14 are connected to the control module 3, the electronic scale is connected to the weighing module, and the weighing module is connected to the control module 3.

[0043] After the first inoculation into the bioreactor 8, turn on the servo motor 14 to deliver the raw materials in the feeding bottle 11 to the inlet end of the reaction tank 8 respectively. At this time, grab and record the reading of the electronic scale, then turn off the servo motor 14, grab and record the reading of the electronic scale at this time, as the initial pipeline feeding value.

[0044] The feeding strategy management module sends the feeding plan to the control module 3.

[0045] Start formal feeding

[0046] First, turn on the servo motor 14, and deliver the relevant raw materials to the entrance of the reaction tank 8 according to the initial pipeline feeding value. At this time, grab and record the reading of the electronic scale, then start the process flow management according to the control module 3 that receives the feeding plan, set the three-stage speed for gradient feeding, when the feeding amount is greater than 10g, the speed of the pressure pump 12 is 150g; when the feeding amount is less than 10g and greater than 2g, the speed of the pressure pump 12 is 80g; when the feeding amount is less than 2g, the speed of the pressure pump 12 is 20g. Then turn off the servo motor 14, grab and record the reading of the electronic scale at this time, as the current pipeline feeding value, and the current feeding is completed.

[0047] Repeat the operation every day and use the last pipeline feeding value as the basic deduction until the end of the culture.

[0048] Daily monitoring and control of module 3 execution log, data connection status and abnormal data capture.

[0049] The contents not described in detail in this specification belong to the prior art known to professional and technical personnel in this field.

[0050] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An automatic feeding device for a bioreactor to prevent external infection, comprising a base (1), wherein a support frame (2) is fixedly mounted on the upper end of the base (1), a control module (3) is fixedly mounted on the upper end of the support frame (2), and a timer (4) is fixedly mounted on the upper end of the support frame (2); Features: Also includes: A first electronic scale (5) is installed on the upper end of the base (1), and the first electronic scale (5) is connected to the upper surface of the base (1) via a suction cup (7); a reaction tank (8) is arranged on the upper end of the first electronic scale (5), and the reaction tank (8) is connected to a feeding bottle (11) via a feeding pipe (13); a clamping assembly is installed on the outer side of the feeding pipe (13), and the feeding pipe (13) is clamped by the clamping assembly to achieve an automatic feeding effect.

2. The automatic feeding device for bioreactors to prevent external infection according to claim 1, characterized in that: A second electronic scale (9) is installed on the upper end of the base (1), and the second electronic scale (9) is connected to the upper surface of the base (1) via a suction cup (7). A monitoring component is installed on the outer side of the second electronic scale (9), and the change of the material can be monitored in real time through the monitoring component, thereby reducing the error between the actual feeding amount and the planned feeding amount.

3. The automatic feeding device for bioreactors against external infection according to claim 1, characterized in that: The clamping assembly comprises a servo motor (14) and a counter-rotating threaded rod (15), wherein the servo motor (14) is fixedly mounted at the bottom of the support frame (2), and the counter-rotating threaded rod (15) is fixedly mounted at the output end of the servo motor (14), and threaded connectors (16) are threadedly connected at both ends of the counter-rotating threaded rod (15), and a clamping plate (17) is fixedly mounted at the lower end of the threaded connector (16), and the clamping plate (17) is distributed in a front-to-back correspondence with respect to the midpoint of the feed pipe (13).

4. The automatic feeding device for bioreactors to prevent external infection according to claim 3, characterized in that: A sliding track (18) is installed at the lower end of the support frame (2), and a limiting rod (19) is nested inside the sliding track (18), and the limiting rod (19) is fixedly installed on the upper end of the clamping plate (17).

5. The automatic feeding device for bioreactors to prevent external infection according to claim 2, characterized in that: The monitoring component comprises a first weighing module (6) and a second weighing module (10), wherein the first weighing module (6) is installed outside the first electronic scale (5), and the second weighing module (10) is installed outside the second electronic scale (9).

6. The automatic feeding device for bioreactors to prevent external infection according to claim 5, characterized in that: The first weighing module (6) and the second weighing module (10) are both connected to the control module (3) via signal lines.

7. The automatic feeding device for bioreactors to prevent external infection according to claim 1, characterized in that: A pressure pump (12) is fixedly mounted on the outside of the feeding bottle (11).

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