Monitoring device for cell incubator

By designing a circulating water replenishment mechanism in the cell incubator, automatic water replenishment is achieved, which solves the problem of inability to replenish water in a cell incubator when cells are monitored in the cell incubator, improves the continuity and stability of cell culture, and saves water resources .

CN222434493UActive Publication Date: 2025-02-07CHENGDU TARGET TECHNOLOGY CO LTD
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Patent Information

Application Number
CN202421117597.3
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2024-05-22
Publication Date
2025-02-07
Estimated Expiration
2034-05-22

AI Technical Summary

Technical Problem

The monitoring device in some cell incubators cannot replenish water in time when monitoring cells are deficient in water, and requires manual intervention, resulting in delayed response and affecting the continuity and stability of cell culture.

Method used

A monitoring device for a cell incubator is designed, including a circulating water replenishment mechanism, including a spray piece, a first water tank, a connecting pipe, a second water tank and a filter plate, to realize automatic water replenishment. When the moisture evaporates cause the humidity in the box to drop, the water is transported from the first water tank to the spray piece and sprayed evenly back into the box to maintain appropriate humidity.

Benefits of technology

Automatic hydration is achieved, manual intervention is reduced, cell culture continuity and stability is improved, water resources is saved, external pollutants have been reduced on the impact of cell culture environment, and system sustainability and economicality are improved.

✦ Generated by Eureka AI based on patent content.

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Abstract

The utility model discloses a monitoring device for a cell culture box, which comprises a culture box body and a circulating water replenishing mechanism arranged below the culture box body, the culture box body comprises a box body, the front side of the box body is movably connected with a glass sliding door, the circulating water replenishing mechanism comprises a spraying piece arranged in the box body, and the spraying piece is movably connected with the glass sliding door. A first water tank is fixedly connected to the top of the spraying piece and fixedly arranged on the top of the box body. The device has the advantage of automatically supplementing water, and overcomes the defects that although monitoring devices in part of cell incubators are powerful in function, some defects still exist in practical application, for example, when it is monitored that the cells are lack of water, the device cannot supplement water for the cells in time, and water still needs to be added through manual intervention, so that response delay is possibly caused, and the cell incubators are damaged. The continuity and the stability of cell culture are influenced.
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Description

Technical Field

[0001] The utility model relates to the technical field of cell culture, in particular to a monitoring device for a cell culture box. Background Art

[0002] Monitoring devices in cell culture incubators are key components to ensure that the cell culture process is carried out under ideal conditions. These devices can monitor and control multiple parameters in the culture environment to maintain the optimal growth state of cells.

[0003] Although the monitoring devices in some cell culture incubators are powerful, they still have some defects in practical applications. For example, when the device detects that the cells are dehydrated, it cannot replenish water to the cells in time and still requires manual intervention to add water, which may cause response delays and affect the continuity and stability of cell culture. Utility Model Content

[0004] The purpose of the utility model is to provide a monitoring device for a cell culture incubator, which has the advantage of automatic water replenishment and solves the problem that although the monitoring devices in some cell culture incubators are powerful, they still have some defects in practical applications. For example, when it is detected that the cells are short of water, the device cannot replenish water for the cells in time and still requires manual intervention to add water, which may cause response delays and affect the continuity and stability of cell culture.

[0005] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a monitoring device for a cell culture box, comprising a culture box body and a circulating water replenishment mechanism arranged below the culture box body, wherein the culture box body comprises a box body, and a glass sliding door is movably connected to the front side of the box body:

[0006] The circulating water replenishment mechanism includes a spray component arranged inside the box body, a first water tank is fixedly connected to the top of the spray component, the first water tank is fixedly arranged on the top of the box body, a connecting pipe is fixedly connected to the top of the first water tank, one end of the connecting pipe is fixedly connected to the water outlet of a water pump, a second water tank is arranged below the box body, a plurality of filter plates are equidistantly arranged inside the second water tank, a water pump is fixedly connected to one side of the second water tank, a water pumping end of the water pump is fixedly connected to a water pumping pipe, and one end of the water pumping pipe is fixedly connected to the bottom of one side of the second water tank.

[0007] As a preferred monitoring device for a cell culture box of the utility model, a rotating shaft is rotatably connected to the center of the box body, a plurality of culture trays are equidistantly sleeved on the surface of the rotating shaft, a humidity monitor is provided inside the culture tray, and the bottom of the rotating shaft extends to the bottom of the box body and is fixedly connected to a power motor.

[0008] As a preferred monitoring device for a cell culture incubator of the utility model, support legs are fixedly connected to the left and right sides of the bottom of the box body, drainage pipes are fixedly connected to the left and right sides of the bottom of the box body, the support legs and the bottom of the drainage pipes are fixedly connected to the top of the second water tank, and the power motor is fixedly arranged on the top of the second water tank.

[0009] As a preferred monitoring device for a cell culture box of the utility model, a plurality of positioning holes are equidistantly provided on the rotating shaft, an opening adapted to fit the positioning holes is provided on the culture plate, and a positioning pin is clamped between the opening and the positioning hole.

[0010] As a preferred monitoring device for a cell culture box of the utility model, slide grooves are correspondingly opened on the front and rear sides of the rotating shaft, and sliders are fixedly connected to the front and rear sides of the inner wall of the culture plate, and the sliders are slidably connected to the slide grooves.

[0011] As a preferred monitoring device for a cell culture box of the utility model, the spray part includes a plurality of water spray pipes equidistantly arranged on the inner wall of the box, a plurality of nozzles are fixedly connected to one side of the water spray pipe, a ring-shaped tube is fixedly connected to the top of the plurality of water spray pipes, and the ring-shaped tube is fixedly arranged above the inner wall of the box.

[0012] As a preferred monitoring device for a cell culture box of the utility model, the center of the annular tube is fixedly connected to a central tube, the top of the central tube is fixedly connected to a water outlet pipe, the top of the water outlet pipe extends to above the box body and is fixedly connected to a first water tank.

[0013] As a preferred monitoring device for a cell culture box of the utility model, a water inlet pipe is fixedly connected to the top of one side of the second water tank.

[0014] Compared with the prior art, the beneficial effects of the utility model are as follows:

[0015] 1. The utility model realizes the automatic replenishment of water in the box body through the combination of the first water tank and the spray part. When the humidity in the box drops due to water evaporation, water is transported from the first water tank to the spray part and evenly sprayed back into the box body to maintain appropriate humidity. The setting of the second water tank and the filter plate means that the used water will be collected and reused after filtration. This not only saves water resources, but also reduces the impact of external pollutants on the cell culture environment, improves the sustainability and economy of the system, and the glass sliding door design on the front side of the box body makes it easy for researchers to observe the cell culture status at any time without opening the glass sliding door, reducing external interference and pollution risks. This device embodies innovative thinking on water management in the cell culture process, and improves the efficiency and quality of cell culture through automatic circulation water replenishment and water quality management.

[0016] 2. The utility model can ensure that the sprayed water evenly covers the entire internal space of the incubator through multiple water spray pipes and nozzles equidistantly arranged on the inner wall of the box, reduce the situation of excessively high or low local humidity, and provide a more stable and consistent growth environment for cells. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a three-dimensional diagram of the utility model;

[0018] Figure 2 It is a cross-sectional view of the utility model;

[0019] Figure 3 It is a structural schematic diagram of the incubator body of the utility model;

[0020] Figure 4 It is a structural schematic diagram of the rotating shaft of the utility model;

[0021] Figure 5 It is a structural schematic diagram of the circulating water replenishment mechanism of the utility model;

[0022] Figure 6 It is a structural schematic diagram of the spraying part of the utility model.

[0023] In the figure: 1. incubator body; 101. box body; 102. supporting legs; 103. drain pipe; 104. rotating shaft; 105. power motor; 106. positioning socket; 107. positioning latch; 108. slide; 109. incubation tray; 110. slider; 2. circulating water replenishment mechanism; 201. spray pipe; 202. nozzle; 203. annular pipe; 204. center pipe; 205. outlet pipe; 206. first water tank; 207. connecting pipe; 208. second water tank; 209. water inlet pipe; 210. filter plate; 211. water pump; 212. suction pipe; 3. glass sliding door. DETAILED DESCRIPTION

[0024] See also Figure 1-Figure 6 A monitoring device for a cell culture box includes a culture box body 1 and a circulating water replenishment mechanism 2 arranged below the culture box body 1. The culture box body 1 includes a box body 101, and a glass sliding door 3 is movably connected to the front side of the box body 101:

[0025] Furthermore, the circulating water replenishment mechanism 2 includes a spray component arranged inside the box body 101, and a first water tank 206 is fixedly connected to the top of the spray component. The first water tank 206 is fixedly arranged on the top of the box body 101, and a connecting pipe 207 is fixedly connected to the top of the first water tank 206. One end of the connecting pipe 207 is fixedly connected to the water outlet end of a water pump 211. A second water tank 208 is provided below the box body 101, and a plurality of filter plates 210 are equidistantly provided inside the second water tank 208. A water pump 211 is fixedly connected to one side of the second water tank 208, and a pumping pipe 212 is fixedly connected to the water pumping end of the water pump 211, and one end of the pumping pipe 212 is fixedly connected to the bottom of one side of the second water tank 208.

[0026] This device realizes automatic replenishment of water in the box 101 by combining the first water tank 206 with the spray part. When the humidity in the box decreases due to evaporation of water, water is transported from the first water tank 206 to the spray part and evenly sprayed back into the box 101 to maintain appropriate humidity. The second water tank 208 and the filter plate 210 are set up, which means that the used water will be collected and reused after filtering. This not only saves water resources, but also reduces the impact of external pollutants on the cell culture environment, and improves the sustainability and economy of the system. The glass sliding door 3 on the front side of the box 101 is designed to facilitate researchers to observe the cell culture status at any time without opening the glass sliding door 3, reducing external interference and pollution risks. This device embodies innovative thinking on water management in the cell culture process, and improves the efficiency and quality of cell culture through automatic circulation water replenishment and water quality management.

[0027] Furthermore, a rotating shaft 104 is rotatably connected to the center of the box 101, and a plurality of cultivation trays 109 are equidistantly sleeved on the surface of the rotating shaft 104. A humidity monitor is provided inside the cultivation tray 109. The bottom of the rotating shaft 104 extends to the bottom of the box 101 and is fixedly connected to a power motor 105.

[0028] Through the rotating shaft 104 at the center of the box 101 and multiple culture trays 109 equidistantly arranged, the cells can experience a three-dimensional culture environment close to the physiological state during the rotation process. The rotating shaft 104 drives the culture trays 109 to rotate through the power motor 105, which helps to evenly distribute environmental factors such as temperature, humidity, and gases such as CO2 and O2 in the box 101, reduce local differences in the culture environment, and ensure that the cells on each culture tray 109 can obtain consistent culture conditions.

[0029] Furthermore, support legs 102 are fixedly connected to the left and right sides of the bottom of the box body 101, and drainage pipes 103 are fixedly connected to the left and right sides of the bottom of the box body 101. The bottoms of the support legs 102 and the drainage pipes 103 are fixedly connected to the top of the second water tank 208, and the power motor 105 is fixedly arranged on the top of the second water tank 208.

[0030] Through the integrated design of the support legs 102, the drain pipe 103 and the top of the second water tank 208, it can be seen that the designer is striving to achieve functional coordination of various parts of the system and maximize space utilization, which not only improves the practicality of the equipment, but also helps to simplify the maintenance process and improve work efficiency.

[0031] Furthermore, a plurality of positioning holes 106 are equidistantly provided on the rotating shaft 104 , and an opening adapted to fit the positioning holes 106 is provided on the cultivation tray 109 , and a positioning pin 107 is clamped between the opening and the positioning hole 106 .

[0032] The positioning pin 107 is fixed to ensure the precise position and stable installation of the culture tray 109 on the rotating shaft 104, which not only avoids the deviation or falling off of the culture tray 109 during rotation, but also facilitates the adjustment of the position of the culture tray 109 according to different experimental requirements to achieve flexible layout.

[0033] Furthermore, slide grooves 108 are correspondingly provided on the front and rear sides of the rotating shaft 104 , and sliders 110 are fixedly connected to the front and rear sides of the inner wall of the cultivation plate 109 , and the sliders 110 are slidably connected to the slide grooves 108 .

[0034] The above design makes the installation and removal of the culture plate 109 simple and quick, and it can be replaced or cleaned without complicated operations, which greatly improves the efficiency of experimental operations and reduces interference with the cell culture environment.

[0035] Furthermore, the spraying part includes a plurality of water spray pipes 201 equidistantly arranged on the inner wall of the box body 101 , a plurality of nozzles 202 are fixedly connected to one side of the water spray pipes 201 , a ring pipe 203 is fixedly connected to the top of the plurality of water spray pipes 201 , and the ring pipe 203 is fixedly arranged above the inner wall of the box body 101 .

[0036] By equidistantly disposing a plurality of water spray pipes 201 and nozzles 202 on the inner wall of the box body 101, it can be ensured that the sprayed water evenly covers the entire internal space of the incubator, reducing the situation where the local humidity is too high or too low, and providing a more stable and consistent growth environment for the cells.

[0037] Furthermore, the center of the annular tube 203 is fixedly connected to a central tube 204 , the top of the central tube 204 is fixedly connected to a water outlet pipe 205 , and the top of the water outlet pipe 205 extends to above the box body 101 and is fixedly connected to a first water tank 206 .

[0038] The annular tube 203 is fixed on the inner wall of the box body 101 and combined with the central tube 204 to form a three-dimensional water spray network. This design not only increases the water spray coverage area, but also connects to the first water tank 206 through the outlet pipe 205 of the central tube 204, ensuring a continuous supply of water and making the water replenishment process smoother.

[0039] Furthermore, a water inlet pipe 209 is fixedly connected to the top of one side of the second water tank 208 .

[0040] The provision of the water inlet pipe 209 ensures that the second water tank 208 can be continuously replenished with clean water from an external water source, which is essential for maintaining the continuous operation of the entire circulating water replenishment system. It allows fresh water to be replenished in a timely manner as needed while the water is recycled, thereby maintaining the stability of the system and the freshness of the water quality.

[0041] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A monitoring device for a cell culture box, comprising a culture box body (1) and a circulating water replenishment mechanism (2) arranged below the culture box body (1), wherein the culture box body (1) comprises a box body (101), and a glass sliding door (3) is movably connected to the front side of the box body (101), characterized in that: The circulating water replenishment mechanism (2) comprises a spraying component arranged inside the box body (101), the top of the spraying component is fixedly connected to a first water tank (206), the first water tank (206) is fixedly arranged on the top of the box body (101), the top of the first water tank (206) is fixedly connected to a connecting pipe (207), one end of the connecting pipe (207) is fixedly connected to a water outlet end of a water pump (211), a second water tank (208) is arranged below the box body (101), a plurality of filter plates (210) are equidistantly arranged inside the second water tank (208), one side of the second water tank (208) is fixedly connected to a water pump (211), the water pumping end of the water pump (211) is fixedly connected to a water pumping pipe (212), one end of the water pumping pipe (212) is fixedly connected to the bottom of one side of the second water tank (208).

2. A monitoring device for a cell culture incubator as claimed in claim 1, characterized in that: The box body (101) is rotatably connected to a rotating shaft (104) at its center, a plurality of cultivation trays (109) are equidistantly sleeved on the surface of the rotating shaft (104), a humidity monitor is provided inside the cultivation tray (109), and the bottom of the rotating shaft (104) extends to the bottom of the box body (101) and is fixedly connected to a power motor (105).

3. A monitoring device for a cell culture chamber as claimed in claim 2, characterized in that: Support legs (102) are fixedly connected to the left and right sides of the bottom of the box body (101), and drainage pipes (103) are fixedly connected to the left and right sides of the bottom of the box body (101). The bottoms of the support legs (102) and the drainage pipes (103) are both fixedly connected to the top of the second water tank (208), and the power motor (105) is fixedly arranged on the top of the second water tank (208).

4. A monitoring device for a cell culture box as claimed in claim 2, characterized in that: The rotating shaft (104) is provided with a plurality of positioning holes (106) at equal intervals, the cultivation plate (109) is provided with openings adapted to the positioning holes (106), and a positioning pin (107) is clamped between the openings and the positioning holes (106).

5. A monitoring device for a cell culture chamber as claimed in claim 4, characterized in that: The rotating shaft (104) is provided with sliding grooves (108) on both the front and rear sides thereof, and the inner wall of the cultivation tray (109) is fixedly connected with sliding blocks (110) on both the front and rear sides thereof, and the sliding blocks (110) are slidably connected to the sliding grooves (108).

6. A monitoring device for a cell culture incubator as claimed in claim 1, characterized in that: The spraying component comprises a plurality of water spray pipes (201) equidistantly arranged on the inner wall of the box body (101), a plurality of spray heads (202) being fixedly connected to one side of the water spray pipes (201), an annular tube (203) being fixedly connected to the top of the plurality of water spray pipes (201), and the annular tube (203) being fixedly arranged above the inner wall of the box body (101).

7. A monitoring device for a cell culture box according to claim 6, characterized in that: The center of the annular tube (203) is fixedly connected to a central tube (204), the top of the central tube (204) is fixedly connected to a water outlet pipe (205), and the top of the water outlet pipe (205) extends to the top of the box body (101) and is fixedly connected to a first water tank (206).

8. A monitoring device for a cell culture box as claimed in claim 1, characterized in that: A water inlet pipe (209) is fixedly connected to the top of one side of the second water tank (208).