Lepidoptera insect larva phototaxis and lucifuge behavior reaction measuring device

Abstract

The utility model discloses a lepidoptera insect larva phototaxis and shading behavior reaction measuring device which comprises a behavior reaction chamber, a top cover, a light barrier, an insect placing box, an LED light source, a lamp holder and a neutral light filter, white filter paper is laid at the bottom of the behavior reaction chamber, the top cover is arranged at the top of the behavior reaction chamber, and the light barrier is arranged on the top of the behavior reaction chamber. The top cover is made of a transparent acrylic organic glass material and is provided with a plurality of hollow holes for air circulation, the light barrier is vertically arranged at the top of the behavior reaction chamber, the behavior reaction chamber is averagely divided into a first side and a second side by taking the light barrier as a boundary, and the insect placing box is arranged at the central position of the behavior reaction chamber; the insect placing box is made of transparent acrylic organic glass materials, the insect placing box is in a circular ring shape with an upper opening and a lower opening, the center point of the insect placing box is overlapped with the center point of the behavior reaction chamber, the LED light source is installed over the phototaxis chamber, the LED light source is supported by the lamp holder, the LED light source is white light or monochromatic light with different wavelengths, and the neutral light filter is arranged on a light path of the LED light source.

Description

Technical Field

[0001] The utility model relates to the field of agricultural pest research, in particular to a device for measuring the phototropism behavior reaction of lepidoptera insect larvae. Background Art

[0002] Insect phototaxis is an important ecological characteristic of insects. Insect phototaxis refers to the tendency of insects to fly towards brighter light. Most agricultural pests are phototactic, and pests show different degrees of phototaxis to light of different light intensities.

[0003] Studying the phototaxis of agricultural pests is of great significance for pest hunting and pest control. Most of the existing experimental equipment are not convenient for studying the phototaxis and photophobia reactions of insects under light of different light intensities, so it is necessary to improve them. Utility Model Content

[0004] The utility model provides a device for measuring the phototropism behavior reaction of lepidoptera insect larvae, which is used to solve the technical problems existing in the prior art.

[0005] In order to achieve the above-mentioned object, the utility model provides a device for measuring the light-avoidance behavior reaction of lepidopteran insect larvae, which comprises a behavior reaction chamber, a top cover, a light shielding plate, an insect box, an LED light source, a lamp holder and a neutral filter, wherein:

[0006] The behavioral reaction chamber is a round glass culture dish with white filter paper on the bottom.

[0007] The top cover is set on the top of the behavioral reaction chamber. The material of the top cover is transparent acrylic organic glass material. The top cover is provided with multiple hollow holes for air circulation.

[0008] The light shield is vertically set up on the top of the behavior reaction chamber, and the behavior reaction chamber is evenly divided into a first side and a second side by the light shield, wherein the first side is a phototaxis chamber, and the second side is a photophobia chamber, and both the phototaxis chamber and the photophobia chamber are semicircular and of the same size.

[0009] The insect box is set at the center of the behavioral reaction chamber. The material of the insect box is transparent acrylic plexiglass. The insect box is in the shape of a ring with upper and lower openings. The center point of the insect box overlaps with the center point of the behavioral reaction chamber.

[0010] The LED light source is installed directly above the phototropism chamber, and is supported by a lamp holder. The LED light source is white light or monochromatic light of different wavelengths. A neutral filter is arranged on the light path of the LED light source. The light emitted by the LED light source is filtered by the neutral filter to obtain white light or monochromatic light of preset light intensity.

[0011] In one embodiment of the present invention, the height of the behavioral reaction chamber is less than 2.5 cm and the diameter is 25 cm.

[0012] In one embodiment of the present invention, the top cover is composed of two semicircles, and the two semicircles correspond to the phototropic chamber and the photophobic chamber respectively.

[0013] In one embodiment of the utility model, the diameter of the insect box is 1 / 4 to 1 / 3 of the diameter of the behavior reaction chamber.

[0014] In an embodiment of the present invention, the height of the lamp holder is 50 cm.

[0015] The device for measuring the phototaxis and photophobia behavior reaction of lepidopteran insect larvae provided by the utility model can adjust the light intensity according to the specific needs of the experiment, is convenient for conducting experiments on the phototaxis and photophobia of insect larvae under different light intensities, and has strong practicality. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0017] Figure 1 A schematic diagram of a device for measuring the phototropism behavior of lepidopteran insect larvae according to an embodiment of the present invention;

[0018] Figure 2 A schematic diagram of a top cover according to an embodiment of the utility model;

[0019] Figure 3 Schematic diagram of the spectral range of LED light source;

[0020] Figure 4 This is the result of the behavioral response of the first-instar larvae of the armyworm to light;

[0021] Figure 5 This is the result of the behavioral response of the 5th instar larvae of the armyworm to light;

[0022] Figure 6 This is the result of the behavioral response of the first-instar larvae of the fall armyworm to light;

[0023] Figure 7 This figure shows the behavioral response of the fifth-instar larvae of the fall armyworm to light. DETAILED DESCRIPTION

[0024] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0025] Figure 1 This is a schematic diagram of a device for measuring the phototropism behavior of lepidopteran insect larvae according to an embodiment of the present invention. Figure 2 This is a schematic diagram of a top cover of an embodiment of the utility model. To facilitate presentation of various components, the top cover is shown separately. Figure 2 In actual use, the top cover should be placed on the top of the behavioral reaction chamber, such as Figure 1 As shown, the utility model provides a device for measuring the phototropism behavior reaction of lepidopteran insect larvae, which includes a behavior reaction chamber, a top cover, a light shield, an insect box, an LED light source, a lamp holder and a neutral filter, wherein:

[0026] The behavior reaction chamber is a round glass culture dish, and the bottom of the behavior reaction chamber is covered with white filter paper. The white filter paper material eliminates the influence of the background color on the light source to a certain extent. In this embodiment, the height of the behavior reaction chamber is 2.5 cm and the diameter is 25 cm. In other embodiments, the height and diameter of the behavior reaction chamber can be set according to the size of the insect body, which is not limited to this embodiment. Among them, the height of the behavior reaction chamber is generally not higher than 2.5 cm.

[0027] The top cover is set on the top of the behavioral reaction chamber. The material of the top cover is transparent acrylic plexiglass material. The top cover is provided with multiple hollow holes for air circulation. The hollow holes ensure the air circulation between the inside and outside of the device and the activity of the test insects. The transparent material facilitates the observation of the test results and improves convenience. Figure 2 As shown, in this embodiment, the top cover is composed of two semicircles, and the two semicircles correspond to the phototropic chamber and the light-avoiding chamber respectively.

[0028] The light shield is vertically set up on the top of the behavior reaction chamber, and the behavior reaction chamber is evenly divided into a first side and a second side by the light shield, wherein the first side is a phototaxis chamber, and the second side is a photophobia chamber, and both the phototaxis chamber and the photophobia chamber are semicircular and of the same size.

[0029] The insect box is set at the center of the behavioral reaction chamber. The material of the insect box is transparent acrylic plexiglass material. The insect box is in the shape of a circular ring with upper and lower openings. The center point of the insect box overlaps with the center point of the behavioral reaction chamber. In this embodiment, the diameter of the insect box is 7 cm. In other embodiments, the diameter of the insect box can be set according to actual needs, not limited to this embodiment. Generally, the diameter of the insect box is 1 / 4 to 1 / 3 of the diameter of the behavioral reaction chamber.

[0030] The LED light source is installed just above the phototropism chamber. The LED light source is supported by a lamp holder. In this embodiment, the height of the lamp holder is 50 cm. In other embodiments, the height of the lamp holder can be adjusted according to actual needs. The LED light source is white light or monochromatic light of different wavelengths, such as Figure 3 The figure shows a schematic diagram of the spectral range of the LED light source. The neutral filter is set on the light path of the LED light source. The light emitted by the LED light source is filtered by the neutral filter to obtain white light or monochromatic light of a preset light intensity, thereby ensuring the accuracy of the light source.

[0031] The steps of using the utility model to measure the phototropism behavior reaction of lepidoptera insect larvae include:

[0032] S1: Set the temperature and relative humidity inside the darkroom to match the test insects used in the experiment;

[0033] The experiment should be conducted in a dark room to avoid the influence of other light. For example, the temperature inside the dark room is set to (27±0.5)℃ and the relative humidity is set to 70%±10%, which is the most suitable temperature for armyworms and fall armyworms. In other experiments, the settings should be based on the type of insects, generally the most suitable temperature and humidity. In addition, the test time should be determined according to the activity pattern of the larvae, and the test time should coincide with the peak period of larval activity.

[0034] S2: Select 10 insect larvae of a specific age as test insects for the experiment;

[0035] In actual operation, transfer the larvae cultured in an artificial climate box (the temperature and humidity of the breeding environment should be the same as that of the darkroom) to a white enamel dish to select healthy and active larvae. Select 10 healthy 1st or 5th instar larvae and gently sweep them into the culture dish with a small brush.

[0036] S3: Turn on the LED light source and select a suitable neutral filter to adjust the light intensity in the phototaxis chamber to a preset light intensity;

[0037] The preset light intensity is selected as one of the following: 10000lx, 1000lx, 100lx, 10lx, 1, 0.1lx and 0.01lx. At the same time, the light-proof room is kept in a dark state.

[0038] S4: Place the selected test insects for the experiment in an insect box, and after continuous illumination with the LED light source for 5 minutes, remove the insect box;

[0039] During actual operation, the experimental insects in the culture dish are gently transferred to the insect box.

[0040] S5: The experimental test insects freely enter the phototaxis chamber or the phototaxis chamber. After 20 minutes, the number of experimental test insects in the phototaxis chamber and the phototaxis chamber is counted, and the value of the selection index PI is calculated according to the following formula:

[0041] Selection index PI = (the number of experimental test insects in the phototaxis chamber - the number of experimental test insects in the phototaxis chamber) / (the number of experimental test insects in the phototaxis chamber + the number of experimental test insects in the phototaxis chamber).

[0042] In order to reduce experimental errors, the selection index P can be calculated by conducting multiple experiments and taking the average value, and the test insects in each experiment are not reused.

[0043] In order to eliminate the influence of factors such as direction and odor, the behavioral response measurement device was rotated 90° clockwise before each experiment, and the inner wall of the behavioral response chamber was wiped with alcohol and the white filter paper at the bottom of the behavioral response chamber was replaced.

[0044] The following is a specific experiment to illustrate the use effect of the utility model:

[0045] Figure 4 This is the result of the behavioral response of the first-instar larvae of the armyworm to light. Figure 5 The results of the behavioral response of the fifth-instar larvae of the armyworm to light are shown in the figure. Figure 4 , Figure 5 As shown, the first-instar larvae of the armyworm showed different behavioral responses to white light of different light intensities. The first-instar larvae showed a light avoidance behavior response to the strong light intensity of 10,000 lx, with a selection index of -0.84; they showed a phototaxis behavior response to the low light intensities of 1,000, 100 and 10 lx, with selection indexes of 0.19, 0.38 and 0.23, respectively. The fifth-instar larvae of the armyworm showed a light avoidance behavior response to the light intensities of 10,000 and 1,000 lx, with selection indexes of -0.61 and -0.13, respectively, and showed a phototaxis behavior response to the light intensities of 100 and 10 lx, with selection indexes of 0.31 and 0.27, respectively.

[0046] Figure 6 This is the result of the behavioral response of the first-instar larvae of the fall armyworm to light. Figure 7 This is the result of the behavioral response of the fifth instar larvae of the fall armyworm to light. Figure 6 , Figure 7As shown in the figure, the first-instar larvae of fall armyworm showed different behavioral responses to white light of different light intensities. The first-instar larvae showed a light avoidance behavior response to the strong light intensity of 10,000 lx, with a selection index of -0.38; they showed a phototaxis behavior response to the low light intensities of 1,000, 100 and 10 lx, with selection indexes of 0.14, 0.30 and 0.31, respectively. The fifth-instar larvae of fall armyworm showed similar behavioral responses to 10,000, 1,000, 100 and 10 lx, with selection indexes of -0.33, 0.1, 0.33 and 0.42, respectively.

[0047] The device for measuring the phototaxis and photophobia behavior reaction of lepidopteran insect larvae provided by the utility model can adjust the light intensity according to the specific needs of the experiment, is convenient for conducting experiments on the phototaxis and photophobia of insect larvae under different light intensities, and has strong practicality.

[0048] Those skilled in the art can understand that the accompanying drawings are only schematic diagrams of an embodiment, and the modules or processes in the accompanying drawings are not necessarily necessary for implementing the present utility model.

[0049] Those skilled in the art can understand that the modules in the device in the embodiment can be distributed in the device in the embodiment according to the description of the embodiment, or can be changed accordingly and located in one or more devices different from the embodiment. The modules in the above embodiment can be combined into one module, or can be further divided into multiple sub-modules.

[0050] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A device for measuring the phototropism behavior of lepidopteran insect larvae, characterized in that: It includes a behavioral reaction chamber, a top cover, a light shield, an insect box, an LED light source, a lamp holder, and a neutral filter, wherein: The behavioral reaction chamber is a round glass culture dish with white filter paper on the bottom. The top cover is set on the top of the behavioral reaction chamber. The material of the top cover is transparent acrylic organic glass material. The top cover is provided with multiple hollow holes for air circulation. The light shield is vertically set up on the top of the behavior reaction chamber, and the behavior reaction chamber is evenly divided into a first side and a second side by the light shield, wherein the first side is a phototaxis chamber, and the second side is a photophobia chamber, and both the phototaxis chamber and the photophobia chamber are semicircular and of the same size. The insect box is set at the center of the behavioral reaction chamber. The material of the insect box is transparent acrylic plexiglass. The insect box is in the shape of a ring with upper and lower openings. The center point of the insect box overlaps with the center point of the behavioral reaction chamber. The LED light source is installed directly above the phototropism chamber, and is supported by a lamp holder. The LED light source is white light or monochromatic light of different wavelengths. A neutral filter is arranged on the light path of the LED light source. The light emitted by the LED light source is filtered by the neutral filter to obtain white light or monochromatic light of preset light intensity.

2. The device for measuring the phototropism behavior of lepidopteran insect larvae according to claim 1, characterized in that: The behavioral chamber has a height of less than 2.5 cm and a diameter of 25 cm.

3. The device for measuring the phototropism behavior of lepidopteran insect larvae according to claim 1, characterized in that: The top cover is composed of two semicircles, which correspond to the phototactic chamber and the photophobic chamber respectively.

4. The device for measuring the phototropism behavior of lepidopteran insect larvae according to claim 2, characterized in that: The diameter of the insect box is 1 / 4 to 1 / 3 of the diameter of the behavioral reaction chamber.

5. The device for measuring the phototropism behavior of lepidopteran insect larvae according to claim 1, characterized in that: The height of the lamp holder is 50cm.

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