Denitrification nitrogen removal complex microbial inoculant strain incubator

By introducing rotating components and oxygen inhibiting components in the denitrification and denitrification compound bacterial culture culture incubator, the oxygen impact problem caused by the lack of oxygen inhibiting mechanism in the prior art is solved, and the reaction rate and culture effect of denitrification bacteria are significantly improved. .

CN222455021UActive Publication Date: 2025-02-11BEIJING TESI ENVIRONMENTAL PROTECTION TECHNOLOGY CO LTD
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Patent Information

Application Number
CN202420527983.3
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2024-03-19
Publication Date
2025-02-11
Estimated Expiration
2034-03-19

AI Technical Summary

Technical Problem

The existing denitrification and denitrification compound bacterial agent culture incubator lacks an oxygen inhibiting mechanism, which causes the oxygen entering the incubator during the opening and closing of the box door to affect the reaction rate of denitrification bacteria, which is not conducive to the cultivation and growth of denitrification bacteria.

Method used

An incubator including a rotating assembly and an oxygen-repressing assembly was designed. The rotating assembly accelerates the contact between denitrifying bacteria and the culture medium through the rotation of the tray. The oxygen-inhibiting assembly uses oxygen absorbers to inhibit the oxygen content in the incubator, including a combination of a drawer, annular atomization nozzle and a liquid pump to quickly inhibit the oxygen content.

Benefits of technology

The reaction rate of denitrifying bacteria and culture medium is increased by rotating the assembly, and the bacterial activity is ensured through the oxygen-inhibiting assembly, avoiding oxygen affecting the bacterial reaction rate, and significantly improving the culture effect of denitrifying bacteria.

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Abstract

The utility model belongs to the technical field of denitrifying nitrogen removal complex microbial inoculant culture, in particular relates to a denitrifying nitrogen removal complex microbial inoculant strain culture box, and aims to solve the problems that the reaction rate of denitrifying bacteria is influenced by oxygen entering the culture box in the opening and closing process of a box door and the culture and growth of the denitrifying bacteria are not facilitated due to the lack of an oxygen inhibition mechanism in the culture box. According to the scheme, the culture box comprises a culture box body, a sealing door is rotationally installed at a box opening of the culture box body, a plurality of trays are arranged in the culture box body, a plurality of culture dishes are placed on the trays, and a rotating assembly is arranged in the culture box body and used for driving the trays to rotate. When the incubator is used, the oxygen absorbent is used for inhibiting the content of oxygen in the incubator body, so that the activity of denitrifying bacteria is ensured, the content of the oxygen in the incubator body is rapidly inhibited through the matched use of the infusion pump and the annular atomizing spray head, and the situation that the reaction rate of the denitrifying bacteria is influenced by the oxygen brought in when the sealing door is opened is avoided.
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Description

Technical Field

[0001] The utility model relates to the technical field of denitrification and denitrification composite bacterial agent cultivation, in particular to a denitrification and denitrification composite bacterial agent strain cultivation box. Background Art

[0002] Denitrification is a biological treatment technology that usually uses specific bacteria to degrade nitrogen compounds in wastewater, and the denitrification composite bacterial agent culture box is a device used to cultivate and proliferate denitrification bacteria.

[0003] After searching, the patent with announcement number CN216998394U discloses a denitrifying denitrification composite bacterial agent strain culture box, which includes a box body, a carrying plate and a culture dish placed on the top of the carrying plate. There are several carrying plates and they are slidably connected inside the box body. The carrying plates are distributed up and down. Several positioning grooves are opened on the top surface of the carrying plate. The bottom of the culture dish is fixedly connected or integrally formed with a positioning column, and the positioning column and the positioning groove cooperate with each other; a plurality of spray pipes are also arranged in the box body, and the spray pipes are located directly above the carrying plate. A plurality of spray heads are arranged at the bottom of the spray pipes, and the spray heads correspond one to one with the positioning grooves in the vertical direction.

[0004] Although the above-mentioned denitrifying denitrification composite bacterial agent culture box has a positioning column fixedly connected or integrally formed at the bottom of the culture dish to prevent the culture dish from sliding and colliding when the supporting plate is pulled out and causing the culture dish to break, oxygen will inhibit the activity of denitrifying bacteria during the denitrification reaction. The culture box lacks an oxygen inhibition mechanism, resulting in oxygen entering the culture box during the opening and closing of the box door, which will affect the reaction rate of the denitrifying bacteria and is not conducive to the cultivation and growth of denitrifying bacteria.

[0005] In view of the above problems, the utility model document proposes a denitrification and denitrification composite bacterial agent culture box. Utility Model Content

[0006] The utility model provides a denitrifying denitrification composite bacterial agent culture box, which solves the problem that the culture box in the prior art lacks an oxygen suppression mechanism, resulting in oxygen entering the culture box during the opening and closing of the box door, which affects the reaction rate of the denitrifying bacteria and is not conducive to the cultivation and growth of the denitrifying bacteria.

[0007] The utility model provides the following technical solutions:

[0008] A denitrification and denitrification composite bacterial agent bacterial culture box, comprising:

[0009] An incubator body, wherein a sealed door is rotatably mounted at the incubator opening of the incubator body, and a plurality of trays are arranged in the incubator body, and a plurality of culture dishes are placed on the trays;

[0010] The rotating assembly is arranged in the incubator body and is used to drive the tray to rotate, thereby promoting the reaction between the denitrifying bacteria and the culture solution;

[0011] The oxygen suppression component is arranged in the incubator body and is used to reduce the oxygen content in the incubator body, thereby ensuring the activity of denitrifying bacteria.

[0012] In one possible design, the rotating assembly includes a transmission rod rotatably mounted on both ends of the inner wall of the incubator body, the center rings of the plurality of trays are fixedly connected to the outer wall of the transmission rod, and a drive motor for driving the transmission rod to rotate is fixedly mounted on the top of the outer wall of the incubator body.

[0013] In a possible design, a plurality of rectangular plugs for plugging into slots at the bottom of the culture dish are fixedly arranged in a circular array on the top of the tray.

[0014] In one possible design, the oxygen suppression component includes a mounting frame fixedly arranged at the bottom of the outer wall of the incubator body, a drawer for containing oxygen absorbent is slidably connected in the mounting frame, an annular atomizing nozzle is fixedly installed at the top of the inner wall of the incubator body, a liquid pump is fixedly installed at the top of the outer wall of the incubator body, the output end of the liquid pump is connected to the input end of the annular atomizing nozzle through a first infusion tube, the input end of the liquid pump is connected to a second infusion tube, the other end of the second infusion tube passes through the bottom of the outer wall of the incubator body and is connected to a hose, and the hose extends into the drawer.

[0015] In a possible design, the bottom of the incubator body is provided with a plurality of penetration holes in a rectangular array for recovering the oxygen absorbent into the drawer.

[0016] In a possible design, an observation window is provided in the center of the sealed door, and tempered glass is embedded in the observation window.

[0017] It should be understood that the above general description and the following detailed description are merely exemplary and do not limit the present invention.

[0018] In the present application, when in use, the driving motor is used to drive the transmission rod to rotate slowly and uniformly, thereby driving the tray to rotate. With the help of the centrifugal force generated by the rotation of the tray, the contact between the denitrifying bacteria in the culture dish and the culture solution is accelerated, thereby increasing the reaction rate of the denitrifying bacteria and the culture solution. As the tray rotates, it is also convenient for the staff to remove the culture dish inside.

[0019] Under normal conditions, the oxygen absorber in the drawer evaporates through the penetration hole and contacts with the oxygen in the incubator body, thereby suppressing the oxygen content in the incubator body and ensuring the activity of the denitrifying bacteria. When the sealed door is opened and then closed, a large amount of oxygen will flow into the incubator body. At this time, the liquid pump is started, and the liquid pump draws the oxygen absorber in the drawer through the hose and the second infusion tube, and delivers it to the annular atomizing nozzle through the first infusion tube, so that the vaporized oxygen absorber fills the incubator body, thereby quickly suppressing the oxygen content in the incubator body and preventing the oxygen brought in by the opening of the sealed door from affecting the reaction rate of the denitrifying bacteria. The oxygen absorber condensed into water droplets will return to the drawer along the penetration hole again, thereby reducing the loss of the oxygen absorber and improving the recycling rate of the oxygen absorber.

[0020] The utility model has the following beneficial effects:

[0021] The utility model provides a rotating component and utilizes the centrifugal force generated by the rotation of the tray to increase the reaction rate of the denitrifying bacteria and the culture solution, and facilitates the staff to remove the culture dish inside.

[0022] The utility model provides an oxygen suppression component and utilizes an oxygen absorber to suppress the oxygen content in the incubator body, thereby ensuring the activity of the denitrifying bacteria. The utility model also uses a liquid pump and an annular atomizing nozzle to quickly suppress the oxygen content in the incubator body, thereby preventing the oxygen brought in by opening the sealed door from affecting the reaction rate of the denitrifying bacteria.

[0023] The oxygen absorber condensed into water droplets in the utility model will return to the drawer again along the penetration hole, thereby reducing the loss of the oxygen absorber and helping to improve the recycling rate of the oxygen absorber. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 A schematic diagram of the three-dimensional structure of a denitrification and denitrification composite bacterial agent culture box provided by an embodiment of the utility model;

[0025] Figure 2 A schematic diagram of the internal structure of a culture box body of a denitrification and denitrification composite bacterial agent culture box provided by an embodiment of the utility model;

[0026] Figure 3 A schematic cross-sectional view of a denitrification and denitrification composite bacterial agent culture box provided in an embodiment of the utility model;

[0027] Figure 4 A schematic diagram of the structure of a tray and a culture dish of a denitrification and denitrification composite bacterial agent culture box provided in an embodiment of the utility model.

[0028] Figure numerals: 1. incubator body; 2. sealing door; 3. observation window; 4. tray; 5. culture dish; 6. rectangular plug; 7. slot; 8. transmission rod; 9. driving motor; 10. annular atomizing nozzle; 11. liquid pump; 12. mounting frame; 13. drawer; 14. first infusion tube; 15. second infusion tube; 16. penetration hole; 17. hose. DETAILED DESCRIPTION

[0029] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0030] In the description of the present invention, it should be understood that the terms "opening", "upper", "middle", "length", "inside" and the like indicating orientation or positional relationship are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the components or elements referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present invention.

[0031] In order to keep the following description of the embodiments of the present invention clear and concise, the present invention omits detailed descriptions of known functions and known components.

[0032] Embodiment 1

[0033] Please refer to Figure 1-4 , a bacterial culture box, comprising:

[0034] An incubator body 1, a sealed door 2 is rotatably mounted at the incubator opening of the incubator body 1, a plurality of trays 4 are arranged in the incubator body 1, and a plurality of culture dishes 5 are placed on the trays 4;

[0035] The rotating assembly is arranged in the incubator body 1, and is used to drive the tray 4 to rotate, thereby promoting the reaction between the denitrifying bacteria and the culture solution. The rotating assembly includes a transmission rod 8 rotatably mounted on both ends of the inner wall of the incubator body 1, and the central rings of the plurality of trays 4 are fixedly connected to the outer wall of the transmission rod 8. A driving motor 9 for driving the transmission rod 8 to rotate is fixedly mounted on the top of the outer wall of the incubator body 1. The driving motor 9 is connected to an external power supply through an external controller and energized. The driving motor 9 is used to drive the transmission rod 8 to rotate slowly and uniformly, thereby driving the tray 4 to rotate. With the help of the centrifugal force generated by the rotation of the tray 4, the contact between the denitrifying bacteria in the culture dish 5 and the culture solution is accelerated, thereby improving the reaction rate between the denitrifying bacteria and the culture solution. In addition, as the tray 4 rotates, it is also convenient for the staff to remove the inner culture dish 5;

[0036] A plurality of rectangular plugs 6 for plugging into the slots 7 at the bottom of the culture dishes 5 are fixedly arranged in a circular array on the top of the tray 4. The slots 7 and the rectangular plugs 6 are used in combination to fix the position of the culture dishes 5 to prevent the culture dishes 5 from colliding with each other during the rotation of the tray 4.

[0037] An oxygen suppression component is arranged in the incubator body 1, and is used to reduce the oxygen content in the incubator body 1, so as to ensure the activity of denitrifying bacteria. The oxygen suppression component includes a mounting frame 12 fixedly arranged at the bottom of the outer wall of the incubator body 1, and a drawer 13 for containing an oxygen absorbent is slidably connected in the mounting frame 12. An annular atomizing nozzle 10 is fixedly installed on the top of the inner wall of the incubator body 1, and a liquid pump 11 is fixedly installed on the top of the outer wall of the incubator body 1. The liquid pump 11 is connected to an external power supply through an external controller and energized. The output end of the liquid pump 11 is connected to the input end of the annular atomizing nozzle 10 through a first infusion tube 14, and the input end of the liquid pump 11 is connected to a second infusion tube 15. The other end of the second infusion tube 15 passes through the bottom of the outer wall of the incubator body 1 and is connected to a hose 17. The hose 17 is inserted into the drawer 13 so as to draw out the drawer 13 to replenish the oxygen absorbent. The bottom of the incubator body 1 is provided with a plurality of penetration holes 16 in a rectangular array for recovering the oxygen absorbent into the drawer 13;

[0038] More specifically, under normal conditions, the oxygen absorber in the drawer 13 evaporates through the penetration hole 16 and contacts with the oxygen in the incubator body 1, thereby suppressing the oxygen content in the incubator body 1 and ensuring the activity of the denitrifying bacteria. When the sealed door 2 is opened and then closed, a large amount of oxygen will flow into the incubator body 1. At this time, the liquid pump 11 is started, and the liquid pump 11 draws the oxygen absorber in the drawer 13 through the hose 17 and the second infusion tube 15, and delivers it to the annular atomizing nozzle 10 through the first infusion tube 14, so that the vaporized oxygen absorber fills the incubator body 1, thereby quickly suppressing the oxygen content in the incubator body 1 and preventing the oxygen brought in by the opening of the sealed door 2 from affecting the reaction rate of the denitrifying bacteria. The oxygen absorber condensed into water droplets will return to the drawer 13 again along the penetration hole 16, thereby reducing the loss of the oxygen absorber and improving the recycling rate of the oxygen absorber.

[0039] It should be noted that an oxygen absorber is a chemical substance used to absorb oxygen in the surrounding environment. Common oxygen absorbers include compounds such as iron oxide and copper oxide. Oxygen absorbers are usually designed in solid or liquid form. The oxygen absorber used in this application is in liquid form.

[0040] The present application can be used for the cultivation of composite denitrification bacterial agents, and can also be used in other fields applicable to the present application.

[0041] Embodiment 2

[0042] Improvements based on Example 1:

[0043] A denitrification and denitrification composite bacterial agent culture box, which is used in the field of denitrification and denitrification composite bacterial agent culture;

[0044] Please refer to Figure 1 An observation window 3 is provided in the center of the sealed door 2, and tempered glass is embedded in the observation window 3. By providing the observation window 3, it is convenient for the staff to observe the internal situation of the incubator body 1 for recording, thereby reducing the number of times the sealed door 2 is opened and reducing the oxygen entering the incubator body 1.

[0045] However, as is well known to those skilled in the art, the working principles and wiring methods of the drive motor 9, the annular atomizing nozzle 10 and the liquid pump 11 are commonplace, and are conventional means or common knowledge, and will not be elaborated here. Those skilled in the art may make any optional selections according to their needs or convenience.

[0046] The above are only specific implementations of the utility model, but the protection scope of the utility model is not limited to them. Any technician familiar with the technical field can easily think of changes or substitutions within the technical scope disclosed by the utility model, which should be included in the protection scope of the utility model; in the absence of conflicts, the embodiments of the utility model and the features in the embodiments can be combined with each other. Therefore, the protection scope of the utility model shall be based on the protection scope of the claims.

Claims

1. A denitrification and denitrification composite bacterial agent culture box, characterized in that: include: An incubator body (1), wherein a sealing door (2) is rotatably mounted at the incubator opening of the incubator body (1), and a plurality of trays (4) are arranged inside the incubator body (1), and a plurality of culture dishes (5) are placed on the trays (4); A rotating assembly is disposed in the incubator body (1) and is used to drive the tray (4) to rotate, thereby promoting the reaction between the denitrifying bacteria and the culture solution; The oxygen suppression component is arranged in the incubator body (1) and is used to reduce the oxygen content in the incubator body (1), thereby ensuring the activity of the denitrifying bacteria.

2. A denitrification and denitrification composite bacterial agent culture box according to claim 1, characterized in that: The rotating assembly comprises a transmission rod (8) rotatably mounted on both ends of the inner wall of the incubator body (1); the central rings of the plurality of trays (4) are fixedly connected to the outer wall of the transmission rod (8); and a driving motor (9) for driving the transmission rod (8) to rotate is fixedly mounted on the top of the outer wall of the incubator body (1).

3. A denitrification and denitrification composite bacterial agent culture box according to claim 2, characterized in that: A plurality of rectangular plug-in blocks (6) for plugging into slots (7) at the bottom of the culture dish (5) are fixedly arranged in a circular array on the top of the tray (4).

4. A denitrification and denitrification composite bacterial agent culture box according to claim 1, characterized in that: The oxygen suppression component comprises a mounting frame (12) fixedly arranged at the bottom of the outer wall of the incubator body (1), a drawer (13) for containing an oxygen absorbent being slidably connected inside the mounting frame (12), an annular atomizing nozzle (10) being fixedly installed at the top of the inner wall of the incubator body (1), a liquid pump (11) being fixedly installed at the top of the outer wall of the incubator body (1), an output end of the liquid pump (11) and an input end of the annular atomizing nozzle (10) being connected via a first infusion tube (14), the input end of the liquid pump (11) being connected to a second infusion tube (15), the other end of the second infusion tube (15) passing through the bottom of the outer wall of the incubator body (1) and being connected to a hose (17), the hose (17) protruding into the drawer (13).

5. A denitrification and denitrification composite bacterial agent culture box according to claim 4, characterized in that: The bottom of the incubator body (1) is provided with a plurality of penetration holes (16) in a rectangular array for recovering the oxygen absorbent into the drawer (13).

6. A denitrification and denitrification composite bacterial agent culture box according to claim 1, characterized in that: An observation window (3) is provided in the center of the sealed door (2), and tempered glass is embedded in the observation window (3).