Cell culture temperature return box

Abstract

The utility model provides a cell culture temperature return box which is characterized in that a temperature return cavity is arranged at the bottom in a box body, a plurality of distribution shells are symmetrically arranged on two inner walls of the box body, first drainage pipes are respectively arranged on the two inner walls of the box body, one ends of the first drainage pipes respectively extend into the distribution shells far away from the temperature return cavity, and the other ends of the first drainage pipes extend into the distribution shells far away from the temperature return cavity. The other end of the first drainage tube sequentially penetrates through the distribution shell and extends into the temperature return cavity; gas taking ports are respectively formed in the first drainage pipes in the distribution shell, and shunting ports are respectively formed in one side, far away from the inner wall of the box body, of the distribution shell; a backflow mechanism and a tray. According to the utility model, the gas with the increased temperature is correspondingly sent to the corresponding position in the box body through the distribution shells correspondingly arranged in the box body, and the temperature returning effect is improved through the distribution shells oppositely arranged, so that uniform temperature returning flow is formed in the box body, and the gas in the box body is sent to the temperature returning cavity to increase the temperature again in cooperation with the backflow mechanism; and gas circulation in the box body is formed, and the temperature is continuously and stably increased.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture boxes, in particular to a cell culture rewarming box. Background Art

[0002] A cell culture rewarming box is a laboratory equipment for biological cell culture, which is used to rewarm frozen biological samples, such as cells, bacteria or other microorganisms, from a low temperature storage state to a suitable growth temperature, and can provide a stable rewarming environment to adjust the temperature range suitable for maintaining cell growth. A Chinese patent (publication number CN117327579A) discloses a cell culture box with an anti-interference function, which is placed in the interior of the placement groove in sequence, and the driving shaft drives the driven member to drive the pusher to push the force block, and the irregular push block in the force block squeezes the connecting rod inward, so that the card block can be clamped in the interior of the card groove, and the topmost placement plate is pushed out from the inner side of the first sealing plate by the pusher of the pusher, so that the placement plate can be quickly taken out, and in the process of taking out, air will not enter the interior of the culture box body, greatly reducing the possibility of air affecting the cell culture results of other culture dishes; however, the internal structure of the box cannot achieve uniform and stable rewarming of the culture dishes in the box, which affects the rewarming effect of cell culture. Therefore, the utility model proposes a cell culture rewarming box to improve the above problems. Utility Model Content

[0003] Therefore, the technical problem to be solved by the utility model is to overcome the defect that the cell culture reheating box in the prior art cannot achieve uniform and stable reheating of the culture dish, thereby affecting its reheating effect, thereby providing a cell culture reheating box.

[0004] In order to solve the above problems, the utility model provides a cell culture incubator, which comprises:

[0005] A box body, wherein an operation port is provided on the front of the box body, an operation door is provided in the operation port, and the operation port and the operation door are detachably connected; a temperature recovery chamber is provided at the bottom of the box body, and a temperature recovery mechanism is provided in the temperature recovery chamber to increase the temperature inside the box body;

[0006] A plurality of distribution shells are symmetrically arranged on the two inner walls of the box body, and a first drainage pipe is also respectively arranged on the two inner walls of the box body, one end of the first drainage pipe respectively extends into the distribution shell away from the temperature recovery chamber, and the other end of the first drainage pipe respectively passes through the distribution shell in sequence and extends into the temperature recovery chamber;

[0007] The first drainage pipe is provided with air intake ports in the distribution shell, and the distribution shell is provided with flow diversion ports on one side away from the inner wall of the box;

[0008] A reflux mechanism is respectively provided on the inner wall of the box body opposite to the operation port, the adjacent reflux mechanisms are connected, and the reflux mechanism close to the temperature return chamber is connected to the temperature return chamber to reflux the gas in the box body into the temperature return chamber;

[0009] A tray is provided with a plurality of distribution shell tops in the box body, and two ends of the tray respectively abut against the two inner walls of the box body.

[0010] Preferably, the distribution shells symmetrically arranged on the two inner walls of the box body are in the same horizontal plane, and the distances between two adjacent distribution shells on the inner wall of the box body on the same side are equal.

[0011] Preferably, the air intake port faces away from and is close to the inner wall of the box, and a plurality of diversion ports are respectively arranged at different angles on a side of the distribution shell away from the inner wall of the box.

[0012] Preferably, the reflux mechanism includes a reflux shell, the reflux shell is provided with a plurality of inner walls of the box body opposite to the operation port, the reflux shell and two distribution shells symmetrically arranged on the two inner walls of the box body are in the same horizontal plane, the adjacent reflux shells and the reflux shell close to the return temperature chamber are connected to the return temperature chamber by a reflux short pipe, and the reflux port is provided on the side of the reflux shell away from the inner wall of the box body.

[0013] Preferably, one-way valves are respectively arranged in the reflux short pipes, and the flow directions of the one-way valves are all from away from the temperature return chamber to close to the temperature return chamber.

[0014] Preferably, a filter screen is provided in the reflux port, the side of the reflux shell close to the inner wall of the box body penetrates the inner wall of the box body, and a cleaning port is provided on the side of the reflux shell penetrating the inner wall of the box body, and the cleaning port is detachably connected with an adaptive movable plate.

[0015] Preferably, a plurality of grooves are evenly arranged on the tray.

[0016] Preferably, the temperature recovery mechanism comprises at least one heater, and the heater is close to one end of the first drainage pipe extending into the temperature recovery chamber.

[0017] Preferably, a base is provided at the bottom of the box body, the interior of the base is a hollow structure, and a plurality of through holes are respectively provided on each surface of the base.

[0018] The cell culture rewarming box provided by the utility model has the following beneficial effects:

[0019] 1. The utility model delivers the gas with the increased temperature to the corresponding position in the box through the corresponding distribution shell arranged in the box, and correspondingly increases the temperature of the gas at the tray at the position, and improves the temperature recovery effect through the oppositely arranged distribution shell, so that a uniform temperature recovery flow is formed in the box, and the gas in the box is sent to the temperature recovery chamber to increase the temperature again in conjunction with the reflux mechanism, forming a gas circulation in the box, and continuously and stably increasing the temperature;

[0020] 2. The utility model also has a gas inlet facing away from the inner wall of the box, and a diversion port in the distribution shell away from the inner wall of the box, so that the gas entering the first drainage tube from the temperature recovery chamber first flows in the first drainage tube, and then is diverted by the sampling port to the corresponding distribution shell, and then further diverted into the box through the diversion ports of multiple distribution shells, forming a relatively uniform distribution in the box, and multiple diversion ports are respectively arranged at different angles on the side of the distribution shell away from the inner wall of the box, so that the gas coming out of the distribution shell is dispersed to multiple angles with the distribution shell as the center, forming a uniform gas flow in the box;

[0021] 3. The utility model also extends one end of the heating fan close to the first drainage pipe into the temperature return chamber to send the heated gas in the temperature return chamber into the first drainage pipe and then into the box body, forming a circulating airflow between the box body and the temperature return chamber. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 It is a schematic diagram of the three-dimensional structure of the general assembly of the utility model;

[0023] Figure 2 It is a schematic diagram of the internal cross-sectional structure of the utility model;

[0024] Figure 3 This is a schematic diagram of the installation of the cleaning port structure of the utility model;

[0025] Figure 4 for Figure 2 A schematic diagram of the structure enlargement in the middle;

[0026] Figure 5 This is a schematic diagram of the installation of the tray structure of the utility model.

[0027] The reference numerals are:

[0028] 1. Box body; 2. Operation port; 3. Operation door; 4. Return temperature chamber; 5. Distribution shell; 6. First drainage pipe; 7. Air intake port; 8. Diversion port; 9. Tray; 10. Return shell; 11. Return short pipe; 12. Return port; 13. Filter; 14. Cleaning port; 15. Movable plate; 16. Groove; 17. Heater; 18. Base; 19. Through hole. DETAILED DESCRIPTION

[0029] like Figure 1-5 As shown, the utility model provides a cell culture rewarming box, which comprises: a box body 1, an operation port 2 is arranged on the front of the box body 1, an operation door 3 is arranged in the operation port 2, and the operation port 2 is detachably connected to the operation door 3; a rewarming chamber 4 is arranged at the bottom of the box body 1, and a rewarming mechanism is arranged in the rewarming chamber 4 to increase the temperature in the box body 1; a plurality of distribution shells are symmetrically arranged on the two inner walls of the box body 1, and a first drainage tube 6 is also respectively arranged on the two inner walls of the box body 1, one end of the first drainage is respectively extended into the distribution shell away from the rewarming chamber 4, and the other end of the first drainage tube 6 is respectively arranged according to The first drainage pipe 6 is provided with an air intake port 7 in the distribution shell, and a diversion port 8 is provided on the side of the distribution shell away from the inner wall of the box body 1; a reflux mechanism is provided on the inner wall of the box body 1 opposite to the operation port 2, and the adjacent reflux mechanisms are connected, and the reflux mechanism close to the return temperature chamber 4 is connected to the return temperature chamber 4 to reflux the gas in the box body 1 to the return temperature chamber 4; a tray 9 is provided with multiple trays in the box body 1, and the two ends of the tray 9 are respectively against the top of the distribution shell of the two inner walls of the box body 1. Figure 1-5As shown, a cell culture rewarming box is provided, in which a rewarming process of cell culture is carried out in a box body 1, an operation port 2 is provided on the front of the box body 1, one end of the operation port 2 and an operation door 3 are hinged, and the other end is detachably connected, and the operation port 2 and the operation door 3 can be opened and closed by using a lock between the operation port 2 and the operation door 3; a rewarming chamber 4 at the bottom of the box body 1 forms an independent space in the box body 1, and a rewarming mechanism in the rewarming chamber 4 raises the temperature of the gas in the rewarming chamber 4, so that the temperature of the gas in the box body 1 is raised, so as to form a rewarming mechanism in the box body 1 The temperature of the cell culture is restored, wherein the distribution shells on the two inner walls of the box 1 respectively deliver the gas with the temperature raised in the temperature restoration chamber 4 to the plurality of distribution shells through the first drainage pipe 6, and the first drainage pipe 6 is provided with a gas inlet 7 in the distribution shell, so that the gas with the temperature raised in the first drainage pipe 6 enters the distribution shell, and then is delivered to the corresponding position in the box 1 through the diversion port 8 on the side of the distribution shell away from the inner wall of the box 1, so that the temperature of each area in the box 1 can be raised, wherein the two sides of the box 1 The distribution shells symmetrically arranged on the inner wall make it possible to raise the temperature of the box 1 in opposite directions to improve the efficiency of raising the temperature in the box 1. The gas after the temperature is raised in the box 1 passes through a number of reflux mechanisms to send the gas in the box 1 to the temperature return chamber 4 to raise the temperature again, forming a gas circulation in the box 1, and continuously raising the temperature to circulate the gas in the entire box 1 to accelerate the temperature return of the cells in the box 1. The tray 9 is arranged according to the position of the distribution shell, and the two ends of the tray 9 respectively abut the top of the distribution shells on the two inner walls of the box 1, so that the gas after the temperature is raised is sent to the corresponding position in the box 1 through the corresponding distribution shells arranged in the box 1, and the temperature of the gas at the tray 9 at this position is correspondingly raised, and the temperature return effect is improved by the oppositely arranged distribution shells, so that a uniform temperature return flow is formed in the box 1, and the reflux mechanism is used to send the gas in the box 1 to the temperature return chamber 4 to raise the temperature again, forming a gas circulation in the box 1, and continuously and stably raising the temperature.

[0030] In some embodiments, the distribution shells symmetrically arranged on the two inner walls of the box body 1 are in the same horizontal plane, and the distances between two adjacent distribution shells on the inner wall of the box body 1 on the same side are equal. Figure 1-5 As shown, the distribution shells on the two inner walls of the box body 1 are on the same horizontal plane, which is convenient for placing the tray 9. At the same time, the distances between adjacent distribution shells on the inner wall of the box body 1 on the same side are equal, so that the trays 9 are evenly arranged in the box body 1. Through the arrangement of the distribution shell, the temperature-elevated gas drawn out from the distribution shell flows out of the dynamic distribution shell evenly.

[0031] In some embodiments, the air inlet 7 faces away from the inner wall of the box body 1, and the flow diversion opening 8 is provided at multiple angles on the side of the distribution shell away from the inner wall of the box body 1. Figure 1-5As shown, the air intake port 7 faces away from the inner wall of the box body 1, and the diversion port 8 is on the distribution shell away from the inner wall of the box body 1, so that the gas entering the first drainage tube 6 from the temperature return chamber 4 is in flow, which prevents the sampling port close to the temperature return chamber 4 from taking away too much gas after the air intake port 7 and the diversion port 8 directly form a coherent connection. The advantage of this arrangement is that the gas now flows in the first drainage tube 6, and then is diverted by the sampling port to the corresponding distribution shell, and then further diverted to the box body 1 through the diversion ports 8 of multiple distribution shells, forming a relatively uniform distribution in the box body 1, and multiple diversion ports 8 are respectively arranged at different angles on the side of the distribution shell away from the inner wall of the box body 1, so that the gas coming out of the distribution shell is dispersed to multiple angles with the distribution shell as the center, forming a uniform gas flow in the box body 1.

[0032] In some embodiments, the reflux mechanism includes a reflux shell 10, the reflux shell 10 is provided with a plurality of inner walls of the box body 1 opposite to the operation port 2, the reflux shell 10 and two distribution shells symmetrically arranged on the two inner walls of the box body 1 are in the same horizontal plane, the adjacent reflux shells 10 and the reflux shells 10 close to the return temperature chamber 4 are connected to the return temperature chamber 4 by a reflux short pipe 11, and the side of the reflux shell 10 away from the inner wall of the box body 1 is provided with a reflux port 12. Figure 1-5 As shown, a plurality of reflux shells 10 are installed on the inner wall of the box body 1 opposite to the operation port 2, and the reflux shells 10 and the distribution shells symmetrically arranged on the two inner walls of the box body 1 are in the same plane, wherein the tray 9 can be abutted against only two distribution shells, or can be abutted against two distribution shells and the corresponding reflux shells 10, so as to ensure the stability of the tray 9; the adjacent reflux shells 10 and the reflux shells 10 close to the return temperature chamber 4 are connected to the return temperature chamber 4 by the reflux short pipe 11, so that the gas in the box body 1 is sent to the return temperature chamber 4 to increase the temperature again, and the gas in the box body 1 enters the reflux shell 10 through the reflux port 12.

[0033] In some embodiments, a one-way valve is provided in each of the reflux short pipes 11, and the flow direction of each of the one-way valves is from away from the temperature return chamber 4 to close to the temperature return chamber 4. Figure 1-5 As shown, a one-way valve is respectively arranged in the reflux short tube 11, and the one-way valve is commercially available, so that the gas can flow in one direction in the reflux pipe, and the flow direction of the one-way valve is from away from the temperature return chamber 4 to close to the temperature return chamber 4, so that the flow direction of the entire reflux mechanism is limited to flowing from the box body 1 to the temperature return chamber 4, and cooperates with the first drainage tube 6 and the distribution shell to form a circulating airflow in the box body 1 and the temperature return chamber 4, which is heated in the temperature return chamber 4 and then sent to the box body 1 to enhance the temperature return effect on the cells in the box body 1.

[0034] In some embodiments, a filter screen 13 is provided in the reflux port 12, the side of the reflux shell 10 close to the inner wall of the box body 1 penetrates the inner wall of the box body 1, and the side of the reflux shell 10 penetrating the inner wall of the box body 1 is respectively provided with a cleaning port 14, and the cleaning port 14 is detachably connected with an adapted movable plate 15. Figure 1-5 As shown, the pore size of the filter 13 of the reflux port 12 can be determined according to actual conditions. The filter 13 removes impurities in the gas in the box body 1 to prevent the impurities from further flowing with the gas. The reflux shell 10 is close to the cleaning port 14 outside the box body 1, and its interior is adapted with a movable plate 15. The movable plate 15 and the cleaning port 14 can be in a snap-fit ​​form to keep the environment inside the box body 1 closed during normal use.

[0035] In some embodiments, a plurality of grooves 16 are evenly arranged on the tray 9. Figure 1-5 As shown, a plurality of grooves 16 are evenly arranged on the tray 9 to place a plurality of cell culture slides or containers, so that the rewarming effect can be improved when the cells are rewarmed in coordination with the arrangement of the structure in the box 1 .

[0036] In some embodiments, the temperature recovery mechanism includes at least one heater 17, and the heater 17 is close to one end of the first drainage pipe 6 extending into the temperature recovery chamber 4. Figure 1-5 As shown, the heater 17 is commercially available and is located close to one end of the first drainage pipe 6 extending into the temperature return chamber 4 to deliver the heated gas in the temperature return chamber 4 into the first drainage pipe 6 and then into the box body 1 to form a circulating airflow between the box body 1 and the temperature return chamber 4.

[0037] In some embodiments, a base 18 is disposed at the bottom of the box 1, the interior of the base 18 is a hollow structure, and each surface of the base 18 is respectively provided with a plurality of through holes 19. Figure 1-5 As shown, the base 18 at the bottom of the box body 1 separates the box body 1 from the placement surface to prevent it from being affected by the humidity and temperature of the placement surface. The box body 1 and the base 18 can be snap-connected, bonded or screwed. The base 18 has a hollow structure inside, and a number of through holes 19 are respectively provided on each surface of the base 18 to allow air to pass through. At the same time, the weight of the box body 1 is reduced, which supports the box body 1 while preventing the placement surface from affecting the box body 1.

[0038] The above is only a preferred embodiment of the utility model, and is not intended to limit the utility model. Any modifications, equivalent replacements and improvements made within the spirit and principles of the utility model should be included in the protection scope of the utility model. The above is only a preferred implementation of the utility model. It should be pointed out that for ordinary technicians in this technical field, several improvements and variations can be made without departing from the technical principles of the utility model, and these improvements and variations should also be regarded as the protection scope of the utility model.

Claims

1. A cell culture incubator, comprising: The box body has an operation port on the front side, an operation door is provided in the operation port, and the operation port and the operation door are detachably connected, and is characterized in that: A temperature recovery chamber is provided at the bottom of the box body, and a temperature recovery mechanism is provided in the temperature recovery chamber to increase the temperature inside the box body; A plurality of distribution shells are symmetrically arranged on the two inner walls of the box body, and a first drainage pipe is also respectively arranged on the two inner walls of the box body, one end of the first drainage pipe respectively extends into the distribution shell away from the temperature recovery chamber, and the other end of the first drainage pipe respectively passes through the distribution shell in sequence and extends into the temperature recovery chamber; The first drainage pipe is provided with air intake ports in the distribution shell, and the distribution shell is provided with flow diversion ports on one side away from the inner wall of the box; A reflux mechanism is respectively provided on the inner wall of the box body opposite to the operation port, the adjacent reflux mechanisms are connected, and the reflux mechanism close to the temperature return chamber is connected to the temperature return chamber to reflux the gas in the box body into the temperature return chamber; A tray is provided with a plurality of distribution shell tops in the box body, and two ends of the tray respectively abut against the two inner walls of the box body.

2. The cell culture incubator according to claim 1, characterized in that: The distribution shells symmetrically arranged on the two inner walls of the box body are in the same horizontal plane, and the distances between two adjacent distribution shells on the inner wall of the box body on the same side are equal.

3. The cell culture incubator according to claim 1, characterized in that: The air intake port faces away from and is close to the inner wall of the box, and a plurality of diversion ports are respectively arranged at different angles on a side of the distribution shell away from the inner wall of the box.

4. The cell culture incubator according to claim 2, characterized in that: The reflux mechanism includes a reflux shell, and the reflux shell is provided with a plurality of inner walls of the box body opposite to the operation port. The reflux shell and two distribution shells symmetrically arranged on the two inner walls of the box body are in the same horizontal plane. The adjacent reflux shells and the reflux shell close to the temperature return chamber are connected to the temperature return chamber by a reflux short pipe, and a reflux port is provided on the side of the reflux shell away from the inner wall of the box body.

5. The cell culture incubator according to claim 4, characterized in that: One-way valves are respectively arranged in the reflux short pipes, and the flow directions of the one-way valves are all from away from the temperature return chamber to close to the temperature return chamber.

6. The cell culture incubator according to claim 4, characterized in that: A filter screen is arranged in the reflux port, the side of the reflux shell close to the inner wall of the box body penetrates the inner wall of the box body, and the side of the reflux shell penetrating the inner wall of the box body is respectively provided with a cleaning port, and the cleaning port is detachably connected with an adaptable movable plate.

7. The cell culture incubator according to claim 4, characterized in that: A plurality of grooves are evenly arranged on the tray.

8. The cell culture incubator according to claim 1, characterized in that: The temperature recovery mechanism includes at least one heater, and the heater is close to one end of the first drainage pipe extending into the temperature recovery chamber.

9. The cell culture incubator according to claim 1, characterized in that: A base is arranged at the bottom of the box body, the interior of the base is a hollow structure, and a plurality of through holes are arranged on each surface of the base.

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