Three-dimensional rotary replaceable culture solution cell microgravity device

Abstract

The utility model discloses a three-dimensional rotary replaceable culture solution cell microgravity device, which comprises a bracket and a square frame, a cell culture solution shell is arranged at the top of the square frame, a cell replacement solution shell is arranged at the bottom of the square frame, a replacement solution inlet is arranged at the bottom of the cell culture solution shell, and a cell culture solution is arranged in the replacement solution inlet. A replacement liquid outlet is formed in the top of the replacement liquid cavity, a liquid outlet is formed in the top of the cell culture liquid shell, and a liquid inlet is formed in the top of the liquid outlet cavity. The check valve I, the check valve II and the pressure pump are respectively arranged on the surfaces of the replacement pipeline and the discharge pipeline, so that the problem of liquid backflow in the rotating process of the square frame is avoided; and meanwhile, the replaced liquid can be stored in the liquid discharging cavity no matter the cell culture liquid shell is at any inclined angle in the liquid replacement process, and the condition of cross contamination of the culture liquid in the cell culture liquid shell is avoided.

Description

Technical Field

[0001] The utility model relates to the technical field of three-dimensional rotary replaceable culture fluid cell microgravity, in particular to a three-dimensional rotary replaceable culture fluid cell microgravity device. Background Art

[0002] Due to the technical limitations of spacecraft, space shuttles and space stations, the cost of carrying cell biological samples is high, and usually astronauts are required to cooperate in manual operation in the middle of the journey to obtain relatively ideal experimental data, so it is difficult to implement and the feasibility is not high. Therefore, it is impractical to only rely on conducting cell biology experiments in a real space environment to study the effects of microgravity. Therefore, a three-dimensional rotating microgravity instrument with replaceable culture medium is needed. For example, the three-dimensional rotating replaceable culture fluid cell microgravity device disclosed in the authorization announcement number CN203878154U includes a cell culture fluid unit, a cell culture fluid replacement unit, an integral support frame with an axis rotation, and a control unit. The control unit first records the initial position of the cell replacement fluid outlet or the cell replacement fluid outlet port, and the control unit controls the first motor in the cell culture fluid unit to rotate at a certain frequency under a simulated microgravity working state, and records the real-time working position of the cell replacement fluid outlet or the cell replacement fluid outlet port by outputting a step length. The control unit controls the integral drive motor with an axis rotation to rotate the cell culture fluid unit and the cell culture fluid replacement unit around the axis, so that the cell culture fluid replacement unit forms a three-dimensional rotation. However, during the rotation process of the device, it is difficult to control the reflux of the culture fluid. Therefore, during the rotation process, the cell culture fluid is usually replaced by rotating 180 degrees. Subsequent rotation may cause the culture fluid that has been replaced to re-enter the cell culture fluid shell. Utility Model Content

[0003] The utility model aims to provide a three-dimensional rotating culture medium replaceable cell microgravity device to solve the problem of possible backflow of cell culture medium during the rotation replacement process in the above-mentioned background technology.

[0004] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a three-dimensional rotating replaceable culture fluid cell microgravity device, comprising a bracket, a gear transmission mechanism is arranged on the inner wall of one side of the bracket, a rotating shaft is rotatably installed at one end inside the bracket, a mouth frame is installed on the surface of the rotating shaft, a cell culture fluid shell is installed on the top of the mouth frame, a cell replacement fluid shell is installed on the bottom of the mouth frame, a partition plate is installed at one end inside the cell replacement fluid shell, a replacement fluid inlet is arranged at the bottom of the cell culture fluid shell, a replacement fluid outlet is arranged on one side of the top of the cell replacement fluid shell, a replacement fluid outlet is arranged on the top of the cell culture fluid shell, and a replacement fluid inlet is arranged on the other side of the top of the cell replacement fluid shell.

[0005] Preferably, the partition plate divides the cell replacement fluid housing into two cavities, namely a replacement fluid cavity and a discharge fluid cavity, and the interior of the replacement fluid cavity is filled with replacement fluid liquid.

[0006] Preferably, the replacement fluid inlet and the replacement fluid outlet are connected via a replacement pipe, and the outlet fluid outlet and the outlet fluid inlet are connected via an outlet pipe.

[0007] Preferably, a check valve 1 is installed on one end of the surface of the replacement pipe, and a pressure pump and a check valve 2 are installed on both ends of the outlet pipe respectively.

[0008] Preferably, a plurality of cell placement racks are installed inside the cell culture fluid shell, the interior of the cell culture fluid shell is filled with culture fluid, a control panel is installed on the outer wall of one side of the rack, and the output end of the control panel is electrically connected to the gear transmission mechanism, check valve 1, check valve 2 and the input end of the pressure pump.

[0009] Preferably, the gear transmission mechanism includes a driven gear installed at one end of the rotating shaft surface and a motor installed on the inner wall of one side of the bracket, the output end of the motor is equipped with a driving gear meshing with the driven gear, and the input end of the motor is electrically connected to the output end of the control panel.

[0010] Compared with the prior art, the beneficial effects of the utility model are as follows: in the three-dimensional rotating replaceable culture fluid cell microgravity device, during the displacement of the cell culture fluid shell and the cell replacement fluid shell, the culture fluid in the cell culture fluid shell flows through the outlet pipe into the outlet fluid cavity, and the replacement fluid in the replacement fluid cavity flows through the replacement pipe into the cell culture fluid shell, thereby completing the replacement of the cell fluid; by installing check valve 1, check valve 2 and a pressure pump on the surfaces of the outlet pipe and the outlet pipe respectively, the problem of liquid reflux during the rotation of the mouth frame is avoided, and at the same time, it is ensured that during the liquid replacement process, no matter what inclination angle the cell culture fluid shell is at, the replaced liquid can be pressurized and stored in the outlet fluid cavity, and no cross contamination will be caused to the culture fluid in the cell culture fluid shell. BRIEF DESCRIPTION OF THE DRAWINGS

[0011] Figure 1 This is a schematic diagram of the main cross-sectional structure of the utility model;

[0012] Figure 2 For this utility model Figure 1 The enlarged structural diagram at A in the middle;

[0013] Figure 3 This is a schematic diagram of the replacement process structure of the utility model;

[0014] Figure 4It is a side structural schematic diagram of the utility model.

[0015] In the figure: 1, bracket; 2, rotating shaft; 3, motor; 4, driven gear; 5, driving gear; 6, mouth frame; 7, control panel; 8, cell culture fluid shell; 9, cell placement rack; 10, culture fluid liquid; 11, cell replacement fluid shell; 12, replacement fluid cavity; 1201, outlet fluid cavity; 13, partition plate; 14, replacement fluid liquid; 15, replacement fluid inlet; 1501, replacement pipeline; 16, check valve 1; 17, replacement fluid outlet; 18, outlet fluid outlet; 1801, outlet pipeline; 19, pressure pump; 20, outlet fluid inlet; 21, check valve 2. DETAILED DESCRIPTION

[0016] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0017] See also Figure 1-4 The utility model provides an embodiment: a three-dimensional rotary replaceable culture fluid cell microgravity device, comprising a bracket 1, a gear transmission mechanism is arranged on the inner wall of one side of the bracket 1, a rotating shaft 2 is rotatably mounted on one end of the bracket 1, a mouth frame 6 is mounted on the surface of the rotating shaft 2, a cell culture fluid housing 8 is mounted on the top of the mouth frame 6, and a cell replacement fluid housing 11 is mounted on the bottom of the mouth frame 6, and the mouth frame 6 is driven to rotate by the gear transmission mechanism, so that the cell culture fluid housing 8 and the cell replacement fluid housing 11 are exchanged in position, so as to facilitate the replacement of the culture fluid;

[0018] A partition plate 13 is installed at one end of the cell replacement fluid housing 11, a replacement fluid inlet 15 is provided at the bottom of the cell culture fluid housing 8, a replacement fluid outlet 17 is provided on one side of the top of the cell replacement fluid housing 11, a replacement fluid outlet 18 is provided on the top of the cell culture fluid housing 8, and a replacement fluid inlet 20 is provided on the other side of the top of the cell replacement fluid housing 11. The two groups of outlets and inlets are connected to each other, so that the cell replacement fluid housing 11 and the cell culture fluid housing 8 can replace the culture fluid conveniently;

[0019] The partition plate 13 divides the cell replacement fluid housing 11 into two cavities, namely, the replacement fluid cavity 12 and the outlet fluid cavity 1201. The interior of the replacement fluid cavity 12 is filled with a replacement fluid liquid 14. During the rotation of the mouth frame 6, the replacement fluid liquid 14 enters the cell culture fluid housing 8, and other liquids in the cell culture fluid housing 8 enter the outlet fluid cavity 1201 to avoid cross contamination.

[0020] The replacement fluid inlet 15 and the replacement fluid outlet 17 are connected through a replacement pipe 1501, and the replacement fluid outlet 18 and the replacement fluid inlet 20 are connected through an outlet pipe 1801. A check valve 16 is installed at one end of the surface of the replacement pipe 1501, and a pressure pump 19 and a check valve 21 are installed at both ends of the outlet pipe 1801. During the liquid replacement process, the check valve 16 and the check valve 21 are used to effectively prevent liquid reflux, thereby ensuring the cleanliness of the culture fluid;

[0021] A plurality of cell placement racks 9 are installed inside the cell culture liquid housing 8, and the interior of the cell culture liquid housing 8 is filled with culture liquid 10. A control panel 7 is installed on the outer wall of one side of the bracket 1, and the output end of the control panel 7 is electrically connected to the gear transmission mechanism, the check valve 1 16, the check valve 2 21 and the input end of the pressure pump 19;

[0022] The gear transmission mechanism includes a driven gear 4 installed at one end of the surface of the rotating shaft 2 and a motor 3 installed on the inner wall of one side of the bracket 1. The output end of the motor 3 is installed with a driving gear 5 meshing with the driven gear 4. The input end of the motor 3 is electrically connected to the output end of the control panel 7. By turning on the motor 3 to drive the driving gear 5 to rotate, the driven gear 4 meshing with the driving gear 5 rotates, thereby making the 2 rotate synchronously, thereby making the cell replacement fluid shell 11 and the cell culture fluid shell 8 perform liquid replacement.

[0023] When the embodiment of the present application is used, the staff first fills the replacement fluid liquid 14 into the replacement fluid cavity 12, and then turns on the gear transmission mechanism, and the gear transmission mechanism drives the rotating shaft 2 to rotate, and then drives the mouth frame 6 to rotate, so that the cell culture fluid shell 8 and the cell replacement fluid shell 11 are exchanged. In the process of displacement of the cell culture fluid shell 8 and the cell replacement fluid shell 11, the culture fluid liquid 10 in the cell culture fluid shell 8 flows through the outlet pipe 1801 into the outlet fluid cavity 1201 as the rotation tilt angle changes, and the replacement fluid liquid 14 in the replacement fluid cavity 12 flows through the replacement pipe 1801. The pipe 1501 enters the cell culture fluid housing 8, thereby completing the replacement of the cell fluid. During the liquid replacement process, the staff simultaneously opens the check valve 16, the check valve 21 and the pressure pump 19 through the control panel 7. The check valve 16 and the check valve 21 prevent the liquid in the cell replacement fluid housing 11 and the cell culture fluid housing 8 from flowing back to each other during the rotation of the mouth frame 6, thereby preventing the cell fluid from being contaminated. At the same time, when the pressure pump 19 makes the cell culture fluid housing 8 at the lowest position during the rotation, the culture fluid to be replaced can also be pressurized into the outlet liquid cavity 1201.

Claims

1. A three-dimensional rotating microgravity cell culture medium replaceable device, characterized in that: The invention comprises a support (1), wherein a gear transmission mechanism is arranged on an inner wall of one side of the support (1), a rotating shaft (2) is rotatably mounted on one end of the support (1), a mouth frame (6) is mounted on the surface of the rotating shaft (2), a cell culture fluid housing (8) is mounted on the top of the mouth frame (6), a cell replacement fluid housing (11) is mounted on the bottom of the mouth frame (6), a partition plate (13) is mounted on one end of the cell replacement fluid housing (11), a replacement fluid inlet (15) is arranged at the bottom of the cell culture fluid housing (8), a replacement fluid outlet (17) is arranged on one side of the top of the cell replacement fluid housing (11), a discharge fluid outlet (18) is arranged on the top of the cell culture fluid housing (8), and a discharge fluid inlet (20) is arranged on the other side of the top of the cell replacement fluid housing (11).

2. The three-dimensional rotating microgravity device for cells with replaceable culture medium according to claim 1, characterized in that: The partition plate (13) divides the cell replacement fluid housing (11) into two chambers, namely a replacement fluid chamber (12) and a discharge fluid chamber (1201), and the interior of the replacement fluid chamber (12) is filled with replacement fluid liquid (14).

3. The three-dimensional rotating microgravity device for cells with replaceable culture medium according to claim 1, characterized in that: The replacement fluid inlet (15) and the replacement fluid outlet (17) are connected via a replacement pipe (1501), and the outlet fluid outlet (18) and the outlet fluid inlet (20) are connected via an outlet pipe (1801).

4. The three-dimensional rotating microgravity device for cells with replaceable culture medium according to claim 3, characterized in that: A check valve 1 (16) is installed at one end of the surface of the replacement pipe (1501), and a pressure pump (19) and a check valve 2 (21) are installed at both ends of the outlet pipe (1801), respectively.

5. The three-dimensional rotating microgravity device for cells with replaceable culture medium according to claim 1, characterized in that: A plurality of cell placement racks (9) are installed inside the cell culture liquid housing (8), and the interior of the cell culture liquid housing (8) is filled with culture liquid (10). A control panel (7) is installed on the outer wall of one side of the bracket (1), and the output end of the control panel (7) is electrically connected to the gear transmission mechanism, the check valve 1 (16), the check valve 2 (21) and the input end of the pressure pump (19).

6. The three-dimensional rotating microgravity device for cells with replaceable culture medium according to claim 1, characterized in that: The gear transmission mechanism comprises a driven gear (4) mounted on one end of the surface of a rotating shaft (2) and a motor (3) mounted on an inner wall of one side of a bracket (1); a driving gear (5) meshing with the driven gear (4) is mounted on the output end of the motor (3); and the input end of the motor (3) is electrically connected to the output end of a control panel (7).

Images