Phage display device capable of achieving partitioned refrigeration

Abstract

The utility model provides a bacteriophage display device capable of realizing partitioned refrigeration, which is provided with a working space, and a large-volume reagent storage unit, a small-volume reagent storage unit and a centrifugal sedimentation device are arranged in the working space. Peltier refrigeration assemblies used for conducting targeted refrigeration on the large-size reagent storage unit, the small-size reagent storage unit and the centrifugal sedimentation device are arranged in the large-size reagent storage unit, the small-size reagent storage unit and the centrifugal sedimentation device respectively, and the hot ends of the Peltier refrigeration assemblies and the water-cooling heat dissipation module form cooling circulation. According to the utility model, the technical defects of low refrigeration efficiency and low refrigeration pertinence of a refrigeration system which is arranged in a larger space and is used for refrigerating the whole working space can be overcome, namely, the Peltier refrigeration components which are independently controlled to operate can be used for refrigerating and regulating the temperature of each unit and device in a pertinence manner, so that the working efficiency is improved, and the working efficiency is improved. And the refrigeration efficiency is higher.

Description

Technical Field

[0001] The utility model belongs to the technical field of phage display equipment design, and in particular relates to a phage display device capable of zoned refrigeration. Background Art

[0002] Phage is a type of tiny life form that infects and replicates inside bacteria. Using synthetic biology tools, the genetic information of phage can be reprogrammed, making phage or infected bacteria an important tool for drug screening, protein optimization, and biomarker screening.

[0003] Phage display technology is one of the two major development platforms for all approved antibody drugs in the world. Due to its mature research system, low cost, and simple operation, phage display technology has attracted widespread attention from researchers and has extremely important commercial value.

[0004] In the field of antibody preparation, phage display technology provides a simple and fast genetic engineering antibody preparation route for fully human recombinant antibodies: a complete set of antibody heavy chain variable region (VH) and light chain variable region (VL) genes are amplified from human immune cells using PCR technology and expressed on the phage surface in the form of fusion proteins.

[0005] Various types of antibodies can be screened through phage display technology. Compared with monoclonal antibodies produced by immunizing animals, monoclonal antibodies produced using this technology have the advantages of higher efficiency and faster production.

[0006] The current phage display technology is realized by composite manual operation of equipment such as full-temperature vibration incubator, centrifuge, cloning panning equipment, etc. The aforementioned equipment has single functions and is independent of each other, and requires operator participation, which leads to the risk of cross contamination during the culture and panning process. The manual participation also leads to manual selection deviation, which reduces the stability of the process and results. In addition, the independent application of single-function modules and the low integration of equipment lead to time-consuming and high-cost screening and display of phages. Therefore, it is urgent to design a phage display device that can integrate the operation requirements of each process of phage display. Among the functional modules arranged in the phage display device, the large-volume reagent storage unit, the small-volume reagent storage unit and the centrifugal sedimentation device all have refrigeration requirements. However, the refrigeration system that can simultaneously refrigerate the entire working space in a larger space has the disadvantages of low refrigeration efficiency and weak refrigeration pertinence. Based on the above-mentioned disadvantages, the present utility model is proposed. Utility Model Content

[0007] Therefore, the technical problem to be solved by the utility model is to provide a phage display device capable of zoned refrigeration, so as to solve the technical problem that a refrigeration system which is set in a large space of the phage display device and simultaneously cools the entire working space has low refrigeration efficiency and poor refrigeration targeting.

[0008] In order to solve the above problems, the utility model provides a phage display device capable of zoned refrigeration, comprising an operating space, wherein the operating space is provided with a large-volume reagent storage unit, a small-volume reagent storage unit and a centrifugal sedimentation device, wherein the large-volume reagent storage unit, the small-volume reagent storage unit and the centrifugal sedimentation device are respectively provided with Peltier refrigeration components for performing targeted refrigeration thereon respectively, and the hot end of the Peltier refrigeration component forms a cooling cycle with a water-cooled heat dissipation module.

[0009] In some embodiments, the water-cooling heat dissipation module includes a first water-cooling cycle, a second water-cooling cycle, and a third water-cooling cycle that operate independently of each other, wherein a heat dissipation cycle is formed between the first water-cooling cycle and the Peltier refrigeration component provided in the centrifugal sedimentation device, a heat dissipation cycle is formed between the second water-cooling cycle and the Peltier refrigeration component provided in the large-volume reagent storage unit, and a heat dissipation cycle is formed between the third water-cooling cycle and the Peltier refrigeration component provided in the small-volume reagent storage unit.

[0010] In some embodiments, the first water-cooling cycle includes two in parallel, each of the first water-cooling cycles includes a first water-cooling pump and a first water-cooling row, the Peltier refrigeration component for targeted cooling of the centrifugal sedimentation device is a first Peltier refrigeration component, the first Peltier refrigeration component includes a first water-cooling box, the hot end of the first Peltier refrigeration component is in the first water-cooling box, and the first water-cooling box, the first water-cooling pump and the first water-cooling row form a heat dissipation cycle.

[0011] In some embodiments, the second water-cooling cycle includes a second water-cooling pump and a second water-cooling row, and the Peltier refrigeration component used for targeted cooling of the large-volume reagent storage unit is a second Peltier refrigeration component, and the second Peltier refrigeration component includes a second water-cooling box, and the hot end of the second Peltier refrigeration component is in the second water-cooling box. The second water-cooling box, the second water-cooling pump and the second water-cooling row form a heat dissipation cycle.

[0012] In some embodiments, the third water cooling cycle includes a third water cooling pump and a third water cooling row, the Peltier refrigeration component used for targeted cooling of the small volume reagent storage unit is a third Peltier refrigeration component, the third Peltier refrigeration component includes a third water cooling box, the hot end of the third Peltier refrigeration component is in the third water cooling box, and the third water cooling box, the third water cooling pump and the third water cooling row form a heat dissipation cycle.

[0013] In some embodiments, the phage display device further comprises a partition wall, a first side wall of the partition wall is in the working space, a second side wall of the partition wall is in the external space, the first side wall is arranged opposite to the second side wall, the first water-cooling radiator, the second water-cooling radiator, and the third water-cooling radiator are all assembled on the second side wall and are arranged in sequence at intervals along the horizontal width direction of the second side wall.

[0014] In some embodiments, the first water-cooling row, the second water-cooling row, and the third water-cooling row are all provided with cooling fans on the side facing the second side wall, and the cooling airflow driven by the cooling fans is independent of the airflow in the working space.

[0015] In some embodiments, the first water cooling box, the second water cooling pump and the third water cooling pump are all assembled on the top wall of the partition wall.

[0016] In some embodiments, the centrifugal sedimentation device includes a centrifugal device support and a centrifugal rotor assembly assembled on the centrifugal device support, the centrifugal rotor assembly is used to place a centrifuge bottle and can be driven to rotate, the centrifugal device support includes a support plate body, a plurality of the first Peltier refrigeration assemblies are assembled on the bottom side of the support plate body, and each of the first Peltier refrigeration assemblies is arranged at intervals along the circumference of the support plate body.

[0017] The utility model provides a phage display device capable of zoned refrigeration, wherein Peltier refrigeration components are respectively arranged for a large-volume reagent storage unit, a small-volume reagent storage unit and a centrifugal sedimentation device in a working space, so as to be able to independently regulate the temperature of the aforementioned units or devices, and to overcome the technical deficiencies of low refrigeration efficiency and weak refrigeration targeting existing in a refrigeration system that is arranged in a larger space and simultaneously refrigerates the entire working space. That is, in the present application, by independently controlling the operation of the Peltier refrigeration components, the aforementioned units and devices can be refrigerated and temperature-controlled in a targeted manner, and the refrigeration efficiency is higher. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 This is a schematic diagram of the internal structure of a phage display device capable of zoned refrigeration according to an embodiment of the utility model at one viewing angle (excluding components such as the outer shell);

[0019] Figure 2 This is a schematic diagram of the internal structure of the phage display device capable of zoned refrigeration according to an embodiment of the utility model from another perspective (without the outer shell and other components);

[0020] Figure 3 for Figure 1 Bottom view of the centrifugal sedimentation device.

[0021] The reference numerals are as follows:

[0022] 3. Centrifugal sedimentation device; 31. Centrifugal device bracket; 311. Bracket plate; 3121. First water cooling box; 361. First water cooling pump; 362. First water cooling row; 4. Large volume reagent storage unit; 41. Second water cooling pump; 42. Second water cooling row; 5. Small volume reagent storage unit; 51. Third water cooling pump; 52. Third water cooling row; 6. Partition wall. DETAILED DESCRIPTION

[0023] See also Figures 1 to 3 As shown, according to an embodiment of the utility model, there is provided a phage display device capable of zoned refrigeration, having an operating space (not labeled in the figure), wherein the operating space is provided with a large-volume reagent storage unit 4, a small-volume reagent storage unit 5 and a centrifugal sedimentation device 3, wherein the large-volume reagent storage unit 4, the small-volume reagent storage unit 5 and the centrifugal sedimentation device 3 are respectively provided with Peltier refrigeration components (not shown in the figure, not labeled) for performing targeted refrigeration thereon respectively, wherein the hot end (not shown in the figure) of the Peltier refrigeration component forms a cooling cycle with a water-cooled heat dissipation module (not labeled in the figure) to transfer the heat of the hot end of the Peltier refrigeration component to the water-cooled heat dissipation module in time and dissipate it, thereby ensuring the refrigeration effect of the Peltier refrigeration component, and it can be understood that the cold end of each of the Peltier refrigeration components is used to cool the corresponding unit or device.

[0024] In this technical solution, Peltier refrigeration components are respectively arranged for the large-volume reagent storage unit 4, the small-volume reagent storage unit 5 and the centrifugal sedimentation device 3 in the working space, so as to be able to independently regulate the temperature of the aforementioned units or devices, and to overcome the technical deficiencies of low refrigeration efficiency and weak refrigeration targeting in a refrigeration system that is arranged in a larger space and simultaneously refrigerates the entire working space. That is, in this application, by independently controlling the operation of the Peltier refrigeration components, the aforementioned units and devices can be refrigerated and temperature-controlled in a targeted manner, and the refrigeration efficiency is higher.

[0025] In some embodiments, the water-cooling heat dissipation module includes a first water-cooling cycle (not labeled in the figure), a second water-cooling cycle (not labeled in the figure) and a third water-cooling cycle (not labeled in the figure) that operate independently of each other, wherein a heat dissipation cycle is formed between the first water-cooling cycle and the Peltier refrigeration component provided in the centrifugal sedimentation device 3, a heat dissipation cycle is formed between the second water-cooling cycle and the Peltier refrigeration component provided in the large-volume reagent storage unit 4, and a heat dissipation cycle is formed between the third water-cooling cycle and the Peltier refrigeration component provided in the small-volume reagent storage unit 5.

[0026] In this technical solution, corresponding water cooling cycles are separately configured for the Peltier refrigeration components in the aforementioned large-volume reagent storage unit 4, small-volume reagent storage unit 5 and centrifugal sedimentation device 3, which can improve the temperature control flexibility of the Peltier refrigeration components in each unit and device, thereby meeting the different refrigeration temperature requirements of each unit and device.

[0027] In a specific embodiment, the first water cooling cycle includes two in parallel, each of which includes a first water cooling pump 361 and a first water cooling row 362, and the Peltier refrigeration component for cooling the centrifugal sedimentation device 3 is a first Peltier refrigeration component, see Figure 3 As shown, the first Peltier cooling assembly includes a first water cooling box 3121, the hot end of the first Peltier cooling assembly is in the first water cooling box 3121, and the first water cooling box 3121, the first water cooling pump 361 and the first water cooling row 362 form a heat dissipation cycle.

[0028] In this technical solution, the first water cooling box 3121, the first water cooling pump 361 and the first water cooling row 362 form a heat dissipation cycle, that is, the cooling water forms a circulating cooling between the above three, thereby realizing efficient cooling of the hot end of the first Peltier refrigeration component. The two parallel first water cooling cycles can further enhance the control effect of the first Peltier refrigeration component on the refrigeration temperature in the centrifugal sedimentation device 3, thereby improving the refrigeration efficiency.

[0029] In some embodiments, the second water-cooling cycle includes a second water-cooling pump 41 and a second water-cooling row 42, and the Peltier refrigeration component for targeted cooling of the large-volume reagent storage unit 4 is a second Peltier refrigeration component, and the second Peltier refrigeration component includes a second water-cooling box (not shown in the figure, not labeled), and the hot end of the second Peltier refrigeration component is in the second water-cooling box, and the second water-cooling box, the second water-cooling pump 41 and the second water-cooling row 42 form a heat dissipation cycle; the third water-cooling cycle includes a third water-cooling pump 51 and a third water-cooling row 52, ​​and the Peltier refrigeration component for targeted cooling of the small-volume reagent storage unit 5 is a third Peltier refrigeration component, and the third Peltier refrigeration component includes a third water-cooling box (not shown in the figure, not labeled), and the hot end of the third Peltier refrigeration component is in the third water-cooling box, and the third water-cooling box, the third water-cooling pump 51 and the third water-cooling row 52 form a heat dissipation cycle. The first water-cooling row 362, the second water-cooling row 42 and the third water-cooling row 52 are all air-to-water radiators, which use the air with a lower external temperature to cool the cooling water flowing in the radiator, and finally transfer the heat in the aforementioned units and devices to the external environment, thereby achieving the purpose of cooling in each unit and device. The aforementioned water-cooling row can specifically be a tube-fin radiator.

[0030] See also Figure 1 and Figure 2 As shown, in a preferred embodiment, the phage display device further comprises a partition wall 6, a first side wall of the partition wall 6 is in the working space, and a second side wall of the partition wall 6 is in the external space, that is, objectively, the partition wall 6 serves as a sealing structure on one side of the working space of the phage display device. In a specific embodiment, a pipette needle capable of moving along its width direction is provided on the first side wall of the partition wall 6, the first side wall is arranged opposite to the second side wall, and the first water-cooling row 362, the second water-cooling row 42, and the third water-cooling row 52 are all assembled on the second side wall, and are arranged in sequence along the horizontal width direction of the second side wall.

[0031] In this technical solution, the water-cooling rows are arranged in a line on the second side wall of the partition wall 6, and the structural design is simple and the layout is reasonable.

[0032] In some embodiments, the first water-cooling row 362, the second water-cooling row 42, and the third water-cooling row 52 are all provided with cooling fans (not shown and not labeled) on the side facing the second side wall, and the cooling airflow driven by the cooling fans is independent of the airflow in the working space, that is, the airflow driven by each cooling fan will not flow through the working space, thereby preventing the nucleic acid in the working space from contaminating the external environment. The aforementioned cooling fans can drive the air circulation speed to further improve the refrigeration efficiency. In some embodiments, the first water-cooling box 3121, the second water-cooling pump 41, and the third water-cooling pump 51 are all assembled on the top wall of the partition wall 6, further improving the rationality of the component layout.

[0033] See Figure 3 As shown, in some embodiments, the centrifugal sedimentation device 3 includes a centrifugal device bracket 31 and a centrifugal rotor assembly (not labeled in the figure) assembled on the centrifugal device bracket 31, the centrifugal rotor assembly is used to place a centrifuge bottle and can be driven to rotate to achieve centrifugal sedimentation of the sample liquid in the centrifuge bottle, the centrifugal device bracket 31 includes a bracket plate 311, a plurality of the first Peltier refrigeration components are assembled on the bottom side of the bracket plate 311, and each of the first Peltier refrigeration components is arranged at intervals along the circumference of the bracket plate 311, and by arranging a plurality of second Peltier refrigeration components on the bracket plate 311, the refrigeration efficiency of the centrifugal sedimentation device 3 can be further improved, and the balance of the cooling capacity in the centrifugal sedimentation device 3 can be limited and improved.

[0034] In a specific embodiment, 18 pieces of 40*40 mm Peltiers are provided for the centrifugal sedimentation device 3 , 4 pieces of 40*80 mm Peltiers are provided for the large-volume reagent storage unit 4 , and 4 pieces of 40*80 mm Peltiers are provided for the small-volume reagent storage unit 1 .

[0035] It is easy for those skilled in the art to understand that, under the premise of no conflict, the above-mentioned advantageous methods can be freely combined and superimposed.

[0036] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements and improvements made within the spirit and principles of the present invention shall be included in the protection scope of the present invention. The above are only preferred implementations of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and variations can be made without departing from the technical principles of the present invention, and these improvements and variations should also be regarded as the protection scope of the present invention.

Claims

1. A phage display device capable of zoned refrigeration, characterized in that: The invention provides an operation space, wherein the operation space is provided with a large-volume reagent storage unit (4), a small-volume reagent storage unit (5) and a centrifugal sedimentation device (3); the large-volume reagent storage unit (4), the small-volume reagent storage unit (5) and the centrifugal sedimentation device (3) are respectively provided with Peltier refrigeration components for performing targeted refrigeration on them respectively; the hot end of the Peltier refrigeration component forms a cooling cycle with a water-cooled heat dissipation module.

2. The phage display device according to claim 1, characterized in that: The water-cooling heat dissipation module comprises a first water-cooling cycle, a second water-cooling cycle and a third water-cooling cycle which operate independently of each other, wherein a heat dissipation cycle is formed between the first water-cooling cycle and the Peltier refrigeration component provided in the centrifugal sedimentation device (3), a heat dissipation cycle is formed between the second water-cooling cycle and the Peltier refrigeration component provided in the large-volume reagent storage unit (4), and a heat dissipation cycle is formed between the third water-cooling cycle and the Peltier refrigeration component provided in the small-volume reagent storage unit (5).

3. The phage display device according to claim 2, characterized in that: The first water cooling cycle includes two in parallel, each of which includes a first water cooling pump (361) and a first water cooling row (362). The Peltier refrigeration component used for targeted cooling of the centrifugal sedimentation device (3) is a first Peltier refrigeration component. The first Peltier refrigeration component includes a first water cooling box (3121). The hot end of the first Peltier refrigeration component is located in the first water cooling box (3121). The first water cooling box (3121), the first water cooling pump (361) and the first water cooling row (362) form a heat dissipation cycle.

4. The phage display device according to claim 3, characterized in that: The second water cooling cycle comprises a second water cooling pump (41) and a second water cooling row (42); the Peltier cooling component used for targeted cooling of the large volume reagent storage unit (4) is a second Peltier cooling component; the second Peltier cooling component comprises a second water cooling box; the hot end of the second Peltier cooling component is located in the second water cooling box; the second water cooling box, the second water cooling pump (41) and the second water cooling row (42) form a heat dissipation cycle.

5. The phage display device according to claim 4, characterized in that: The third water cooling cycle comprises a third water cooling pump (51) and a third water cooling row (52); the Peltier cooling component used for targeted cooling of the small volume reagent storage unit (5) is a third Peltier cooling component; the third Peltier cooling component comprises a third water cooling box; the hot end of the third Peltier cooling component is located in the third water cooling box; the third water cooling box, the third water cooling pump (51) and the third water cooling row (52) form a heat dissipation cycle.

6. The phage display device according to claim 5, characterized in that: It also includes a partition wall body (6), the first side wall of the partition wall body (6) is in the working space, the second side wall of the partition wall body (6) is in the external space, the first side wall and the second side wall are arranged opposite to each other, the first water-cooling radiator (362), the second water-cooling radiator (42), and the third water-cooling radiator (52) are all assembled on the second side wall, and are arranged in sequence along the horizontal width direction of the second side wall.

7. The phage display device according to claim 6, characterized in that: The first water-cooling radiator (362), the second water-cooling radiator (42), and the third water-cooling radiator (52) are all provided with cooling fans on the side facing the second side wall, and the cooling airflow driven by the cooling fans is independent of the airflow in the working space.

8. The phage display device according to claim 6, characterized in that: The first water cooling box (3121), the second water cooling pump (41) and the third water cooling pump (51) are all assembled on the top wall of the partition wall (6).

9. The phage display device according to claim 3, characterized in that: The centrifugal sedimentation device (3) comprises a centrifugal device support (31) and a centrifugal rotor assembly assembled on the centrifugal device support (31), wherein the centrifugal rotor assembly is used to place a centrifugal bottle and can be driven to rotate, the centrifugal device support (31) comprises a support plate body (311), a plurality of the first Peltier refrigeration assemblies are assembled on the bottom side of the support plate body (311), and each of the first Peltier refrigeration assemblies is arranged at intervals along the circumference of the support plate body (311).

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