Novel cell culture deep hole plate
By adopting a matching design between semi-permeable membrane tube and culture tube on the deep-well plate of cell culture, the cell damage and contamination caused by frequent centrifugation operations when replacing culture medium in the prior art is solved, and a more efficient cell culture process is achieved.
Patent Information
- Application Number
- CN202421020653.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-05-11
- Publication Date
- 2025-03-04
- Estimated Expiration
- 2034-05-11
AI Technical Summary
Existing cell culture plates require frequent centrifugation when replacing the culture medium, which can easily lead to cell damage and contamination, which will affect the experimental results.
A new type of deep-well cell culture plate was designed, and used with semipermeable membrane tubes and culture tubes. The semipermeable membrane tube can be put into the culture tube for cell culture. When changing liquid, the semipermeable membrane tubes are lifted to retain cells and avoid centrifugation.
By reducing centrifugation, cell damage and contamination are avoided, experimental efficiency is improved, and cells on the same plate can be changed at the same time, reducing the operation steps.
Smart Images
Figure CN222556957U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of cell culture plates, in particular to a novel cell culture deep-well plate. Background Art
[0002] Uses of cell culture plates:
[0003] Providing a cell growth environment: The cell culture plate provides a suitable growth environment for cells, including appropriate attachment surface, culture medium, nutrients, temperature, humidity and gas exchange conditions. This environment helps cells to attach, grow, divide and reproduce.
[0004] Observe cell growth: The design of the cell culture plate makes it very convenient to observe cell growth under a microscope. By observing the morphology, quantity, growth rate and distribution of cells, researchers can understand the growth status of cells and conduct subsequent experimental analysis.
[0005] Conduct cell experiments: Cell culture plates can be used for various cell experiments, such as drug screening, toxicity testing, genetic engineering, and protein expression. By culturing cells in culture plates, researchers can study the cell's response to drugs, evaluate the efficacy and safety of drugs, and explore the gene expression and protein synthesis of cells under specific conditions.
[0006] Cell culture needs to be carried out in a sterile clean operating table, and the current cell culture plate is divided into two parts, the lid and the culture plate base. When culturing suspended cells, each time the culture medium is changed, it is necessary to open the lid first, then transfer the culture medium containing the cells to the centrifuge tube for centrifugation, aspirate and discard the supernatant after centrifugation, replace the new culture medium with the centrifuge tube to suspend the cells, and then put the new culture medium and its cells back into the culture plate for culture. Because the culture plate has multiple holes, each time the liquid is changed, it is necessary to centrifuge and change the liquid one by one. The operation process is not only easy to cause cell damage but also cause cell contamination. Because the centrifuge is large and cannot be placed in the clean operating table, the centrifuge tube containing the cells needs to be taken outside the clean operating table for centrifugation each time, which is easy to cause cell contamination and lead to experimental failure. Therefore, a new type of cell culture deep well plate is needed to improve the above problems. Utility Model Content
[0007] In order to solve the problem that current cell culture needs to be carried out in a sterile clean operating table, the current cell culture plate is divided into two parts, a lid and a culture plate base. When culturing suspended cells, each time the culture medium is changed, the lid needs to be opened first, and then the culture medium containing the cells is transferred to a centrifuge tube for centrifugation, the supernatant is sucked and discarded after centrifugation, and then a new culture medium is replaced to suspend the cells in the centrifuge tube, and then the new culture medium and its cells are put back into the culture plate for culture. Because the culture plate has multiple holes, each time the liquid is changed, a hole-by-hole centrifugal operation and liquid change are required, which not only easily causes cell damage but also cell contamination during the operation. Because the centrifuge is large and cannot be put into the clean operating table, the centrifuge tube containing cells needs to be taken to the outside of the clean operating table for centrifugation each time, which easily causes cell contamination, and then leads to the problem of experimental failure. The purpose of the utility model is to provide a novel cell culture deep well plate to solve the problems raised in the above-mentioned background technology.
[0008] In order to achieve the above purpose, the utility model provides the following technical solutions:
[0009] A novel cell culture deep well plate comprises a main body, a collection component is arranged on the top of the main body, and a protection component is fixedly connected to the top of the collection component;
[0010] The main body comprises a receiving bottom plate, a culture tube is arranged on the top of the receiving bottom plate, and the culture tube is provided with a plurality of;
[0011] The collection assembly includes a supporting horizontal plate, the interior of the supporting horizontal plate is fixedly connected with a semipermeable membrane tube, the semipermeable membrane tube is matched with a culture tube, the semipermeable membrane tube can be inserted into the corresponding culture tube for cell culture, and the side of the supporting horizontal plate is fixedly connected with a squeak buckle;
[0012] The protection component comprises a storage box, an elastic slot box is arranged inside the storage box, a side surface of the elastic slot box is fixedly connected with a stretch plate, and a side surface of the stretch plate is fixedly connected with a Velcro tape.
[0013] As a preferred solution of the utility model, several semipermeable membrane tubes are provided, the bottom of the semipermeable membrane tubes is a 0.22 μm microporous filter membrane, the Velcro and the snap fastener are used in combination, and a handle is fixedly connected to the top of the elastic slot box.
[0014] As a preferred solution of the utility model, the top of the receiving base plate is fixedly connected to a first pipe sleeve, the top of the first pipe sleeve is fixedly connected to a support column, and the top of the support column is fixedly connected to a second pipe seat.
[0015] As a preferred solution of the utility model, four of the first pipe sleeves, support columns and second pipe seats are provided.
[0016] As a preferred solution of the utility model, a mounting plate is fixedly connected to the top of the second tube seat, a limiting sleeve is fixedly connected to the interior of the mounting plate, and the culture tube is arranged inside the limiting sleeve.
[0017] As a preferred solution of the utility model, a handle is fixedly connected to the side of the mounting plate, two handles are provided, and a plurality of limiting sleeves are provided.
[0018] As a preferred solution of the utility model, a bottom groove is provided inside the receiving bottom plate, and the bottom groove is provided with a plurality of grooves. An adjusting support rod is fixedly connected to the bottom of the receiving bottom plate.
[0019] As a preferred solution of the utility model, the bottom of the adjusting support rod is fixedly connected with a ground foot, and four adjusting support rods and ground feet are provided.
[0020] Compared with the prior art, the beneficial effects of the utility model are:
[0021] 1. In the present invention, especially for the culture of suspended cells, the semipermeable membrane tube can be used to pass liquid and retain solids (such as cells). The semipermeable membrane tube is placed in the culture tube to culture cells. When the liquid needs to be changed, the semipermeable membrane tube is lifted, the cells are retained in the semipermeable membrane tube, and then the culture medium in the culture tube is replaced, so that the cells do not need to be centrifuged, thereby avoiding multiple centrifugation operations that cause cell contamination to affect the experimental results, and greatly improving the experimental efficiency. Moreover, the same plate cells can be changed at the same time, and there is no need to centrifuge and change the liquid for each well of cells separately, which can avoid cell damage caused by centrifugation, and can also avoid cell contamination and improve cell culture efficiency.
[0022] 2. In the utility model, by using the coordinated use of the elastic trough box and the stretch plate, a plurality of semipermeable membrane tubes can be covered, thereby preventing pollutants from falling into the semipermeable membrane tubes, causing cell contamination and affecting the experimental results. The Velcro composed of Velcro and snap fasteners can reduce costs, and the elastic trough box, the stretch plate, the Velcro and the novel cell culture deep well plate can be sterilized as a whole, which is easy to operate and convenient for experiments. BRIEF DESCRIPTION OF THE DRAWINGS
[0023] Figure 1 It is a schematic diagram of the overall structure of the utility model;
[0024] Figure 2 It is a schematic diagram of the exploded structure of the utility model;
[0025] Figure 3 This is a schematic diagram of the structure of the protection component of the utility model;
[0026] Figure 4 This is a schematic diagram of the support assembly structure of the utility model.
[0027] In the figure: 1. Main body; 101. Supporting bottom plate; 102. Adjusting support rod; 103. Foot; 104. Bottom groove; 105. First tube sleeve; 106. Support column; 107. Second tube seat; 108. Mounting plate; 109. Culture tube; 110. Limiting sleeve; 111. Handle; 2. Collection component; 201. Supporting horizontal plate; 202. Semipermeable membrane tube; 3. Protection component; 301. Storage box; 302. Elastic slot box; 303. Pull handle; 304. Extension plate; 305. Velcro; 306. Squeak buckle. DETAILED DESCRIPTION
[0028] The technical solutions in the embodiments of the utility model will be clearly and completely described below in conjunction with the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the utility model.
[0029] Example: See Figure 1-Figure 4 A novel cell culture deep well plate shown includes a main body 1, a collection component 2 is arranged on the top of the main body 1, and a protection component 3 is fixedly connected to the top of the collection component 2;
[0030] In this embodiment, refer to Figure 1 , Figure 2 and Figure 3 As shown, the main body 1 includes a supporting bottom plate 101, a culture tube 109 is arranged on the top of the supporting bottom plate 101, and a plurality of culture tubes 109 are arranged. The collection component 2 includes a supporting horizontal plate 201, and a semipermeable membrane tube 202 is fixedly connected to the interior of the supporting horizontal plate 201. The semipermeable membrane tube 202 is arranged in a corresponding matching manner with the culture tube 109, and the semipermeable membrane tube 202 can be inserted into the corresponding culture tube 109 for cell culture. The side of the supporting horizontal plate 201 is fixedly connected with a squeak buckle 306. The protection component 3 includes a storage box 301, and an elastic slot box 302 is arranged inside the storage box 301. The elastic slot box 302 02 is fixedly connected to a stretch plate 304, and a Velcro 305 is fixedly connected to the side of the stretch plate 304. The 0.22 μm microporous filter membrane at the bottom of the semipermeable membrane tube 202 can pass liquid and retain solid (such as cells). The semipermeable membrane tube 202 is put into the culture tube 109 to culture cells. When the liquid needs to be changed, the semipermeable membrane tube 202 is lifted and the cells are retained at the bottom of the semipermeable membrane tube 202. This can avoid cell damage and contamination caused by multiple centrifugal liquid replacement operations to affect the experimental results, and greatly improve the experimental efficiency, especially for the culture of suspended cells.
[0031] Among them, the semipermeable membrane tube 202 is provided with several microporous filter membranes of 0.22μm at the bottom of the semipermeable membrane tube 202, the Velcro 305 and the snap fastener 306 are used in combination, and the top of the elastic groove box 302 is fixedly connected with a pull handle 303. By using the elastic groove box 302 and the stretch plate 304 in combination, several semipermeable membrane tubes 202 can be covered, so as to prevent pollutants from falling into the semipermeable membrane tube 202, causing cell contamination and affecting the experimental results. The Velcro composed of the Velcro 305 and the snap fastener 306 and the new cell culture deep well plate as a whole can be sterilized before use, which can ensure sterile operation and avoid contamination of cultured cells. It is convenient to operate and is efficient and safe.
[0032] In this embodiment, refer to Figure 1 and Figure 4 As shown, the top of the receiving base plate 101 is fixedly connected to a first pipe sleeve 105, the top of the first pipe sleeve 105 is fixedly connected to a support column 106, the top of the support column 106 is fixedly connected to a second pipe seat 107, four first pipe sleeves 105, support columns 106 and second pipe seats 107 are provided, the top of the second pipe seat 107 is fixedly connected to a mounting plate 108, the interior of the mounting plate 108 is fixedly connected to a limiting sleeve 110, the culture tube 109 is arranged inside the limiting sleeve 110, and the side of the mounting plate 108 is fixedly connected to the limiting sleeve 110. A handle 111 is fixedly connected, two handles 111 are provided, a plurality of limiting sleeves 110 are provided, a bottom groove 104 is opened inside the supporting base plate 101, a plurality of bottom grooves 104 are opened, an adjusting support rod 102 is fixedly connected to the bottom of the supporting base plate 101, a foot 103 is fixedly connected to the bottom of the adjusting support rod 102, four adjusting support rods 102 and foot 103 are provided, and the coordinated use of the adjusting support rod 102 and the foot 103 can adjust the overall height of the device to suit different environments and needs.
[0033] In the present scheme, a novel deep-well cell culture plate needs to be sterilized as a whole before use, and then the Velcro 305 and the snap fastener 306 are separated in a clean operating table, the stretch plate 304 will rebound and rise, and the elastic slot box 302 is folded by pulling the handle 303, and the elastic slot box 302 is stored in the storage box 301, and the semipermeable membrane tube 202 is put into the culture tube 109 for culturing suspended cells. When the cells need to be replaced, the semipermeable membrane tube 202 is lifted up, and the cells are trapped at the bottom of the semipermeable membrane tube 202, and then the culture medium in the culture tube 109 is replaced, thereby eliminating the steps of centrifugation and aspirating the supernatant, better protecting the cells, and avoiding cell damage and contamination caused by operations such as centrifugation, ensuring the experimental effect of suspended cell culture, and improving cell culture efficiency.
[0034] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.
Claims
1. A novel cell culture deep well plate, comprising a main body (1), characterized in that: A collection component (2) is arranged on the top of the main body (1), and a protection component (3) is fixedly connected to the top of the collection component (2); The main body (1) comprises a receiving bottom plate (101), a culture tube (109) is arranged on the top of the receiving bottom plate (101), and the culture tube (109) is provided with a plurality of; The collection component (2) comprises a supporting transverse plate (201), the interior of the supporting transverse plate (201) is fixedly connected with a semipermeable membrane tube (202), the semipermeable membrane tube (202) is arranged in a corresponding matching manner with the culture tube (109), the semipermeable membrane tube (202) can be inserted into the corresponding culture tube (109) for cell culture, and the side of the supporting transverse plate (201) is fixedly connected with a snap buckle (306); The protection component (3) comprises a storage box (301), an elastic slot box (302) is arranged inside the storage box (301), a side surface of the elastic slot box (302) is fixedly connected to a stretch plate (304), and a side surface of the stretch plate (304) is fixedly connected to a Velcro tape (305).
2. A novel cell culture deep well plate according to claim 1, characterized in that: The semipermeable membrane tube (202) is provided with a plurality of them, the bottom of the semipermeable membrane tube (202) is a 0.22 μm microporous filter membrane, the Velcro (305) and the snap fastener (306) are used in combination, and the top of the elastic slot box (302) is fixedly connected with a handle (303).
3. A novel cell culture deep well plate according to claim 1, characterized in that: The top of the receiving bottom plate (101) is fixedly connected to a first pipe sleeve (105), the top of the first pipe sleeve (105) is fixedly connected to a support column (106), and the top of the support column (106) is fixedly connected to a second pipe seat (107).
4. A novel cell culture deep well plate according to claim 3, characterized in that: Four of the first pipe sleeves (105), the support columns (106) and the second pipe bases (107) are provided.
5. A novel cell culture deep well plate according to claim 3, characterized in that: The top of the second tube seat (107) is fixedly connected to a mounting plate (108), the interior of the mounting plate (108) is fixedly connected to a limiting sleeve (110), and the culture tube (109) is arranged inside the limiting sleeve (110).
6. A novel cell culture deep well plate according to claim 5, characterized in that: A handle (111) is fixedly connected to the side of the mounting plate (108), two handles (111) are provided, and a plurality of limiting sleeves (110) are provided.
7. A novel cell culture deep well plate according to claim 1, characterized in that: A bottom groove (104) is provided inside the receiving bottom plate (101), and a plurality of bottom grooves (104) are provided therein. An adjusting support rod (102) is fixedly connected to the bottom of the receiving bottom plate (101).
8. A novel cell culture deep well plate according to claim 7, characterized in that: The bottom of the adjusting support rod (102) is fixedly connected with a ground foot (103), and four adjusting support rods (102) and four ground feet (103) are provided.