Multifunctional organ-like micro-fluidic chip

Abstract

The utility model discloses a multifunctional organoid micro-fluidic chip, which comprises a substrate layer and a cover plate layer hermetically mounted on the upper end face of the substrate layer, the substrate layer is provided with a liquid inlet part, a liquid mixing part and a liquid outlet part, the liquid inlet part, the liquid mixing part and the liquid outlet part are sequentially communicated, the liquid outlet part comprises a liquid discharge section and a detection section, and the liquid discharge section is communicated with the detection section. The liquid discharge section and the detection section are symmetrically arranged on the two sides of the liquid mixing part, and both the liquid discharge section and the detection section are communicated with the liquid mixing part. The liquid inlet part is arranged on the substrate layer, and the liquid inlet part is designed to be of a structure with the premixing groove and the flow dividing pipelines, so that the liquid inlet quantity can be flexibly selected according to needs during use, secondary mixing of liquid can be realized through the arrangement of the premixing groove and the liquid mixing part, and the liquid mixing effect is improved; and finally, the liquid outlet part is designed into two discharge flow channels, namely the liquid discharge section and the detection section, so that the liquid outlet part can be selectively opened according to the liquid supply requirement or the exposure observation requirement during use.

Description

Technical Field

[0001] The utility model relates to the technical field of microfluidic chips, in particular to a multifunctional organoid microfluidic chip. Background Art

[0002] A microfluidic chip is a device that integrates basic operating units such as sample preparation, reaction, separation, and detection in biological, chemical, and medical analysis processes onto a micron-scale chip. Microfluidic detection chips generally have the advantages of low sample consumption, fast detection speed, easy operation, multi-functional integration, small size, and easy portability. The organoid microfluidic chip is one of the micro-organs with biological functionality.

[0003] The existing Chinese patent with publication number CN117225490A discloses a microfluidic chip, which belongs to the field of microfluidic technology. Aiming at the problem that the mixing uniformity and mixing efficiency of the microfluidic chip are low, resulting in low qualified rate and production efficiency of the prepared nanoparticles, the present application provides a microfluidic chip, including a chip body, a flow channel is arranged on the chip body, and the flow channel includes a liquid inlet and a mixing part; the liquid inlet includes two liquid inlet sections, so as to respectively pass two types of fluids; the mixing part includes a drainage section, a guide section and a mixing section connected in sequence, the guide section includes a lower confluence area and an upper confluence area, the inlet of the guide section is connected to the drainage section, and is arranged opposite to the lower confluence area.

[0004] Regarding the above-mentioned related technologies, it is found that the flow channel structure in the above-mentioned microfluidic chip can only mix two fluids. It cannot be used when multiple fluids need to be mixed or the liquid needs to be replaced and mixed. Moreover, the liquid outlet can only discharge the liquid normally, and observation and irradiation experiments cannot be performed. The function and application range are relatively narrow. Utility Model Content

[0005] The present application provides a multifunctional organoid microfluidic chip for solving the problems raised in the background technology.

[0006] The utility model is achieved in this way:

[0007] A multifunctional organoid microfluidic chip comprises a substrate layer and a cover layer sealed and mounted on the upper end surface of the substrate layer, wherein a liquid inlet portion, a liquid mixing portion and a liquid outlet portion are provided on the substrate layer, wherein the liquid inlet portion, the liquid mixing portion and the liquid outlet portion are sequentially connected, and the liquid outlet portion comprises a liquid discharge section and a detection section, wherein the liquid discharge section and the detection section are symmetrically arranged on both sides of the liquid mixing section, and both the liquid discharge section and the detection section are connected to the liquid mixing section, wherein a liquid inlet pipe corresponding to the liquid inlet portion, a liquid discharge pipe corresponding to the liquid discharge section and a transparent cover corresponding to the detection section are arranged on the cover layer, and a sewage discharge pipe group is also installed at the end of the detection section.

[0008] Furthermore, the liquid inlet portion includes a premixing tank and a plurality of diversion pipes, the plurality of diversion pipes are evenly arranged on one side of the premixing tank, and the diversion pipes are connected to the premixing tank.

[0009] Furthermore, the liquid mixing part includes a lower row trough, an upper rising trough and a lower curved trough, the lower row trough, the upper rising trough and the lower curved trough are connected in sequence, and the head of the lower row trough is connected to the premixing trough, and the lower end of the lower curved trough is connected to the liquid outlet part.

[0010] Furthermore, the drainage section includes a left arc groove and a straight groove corresponding to the drainage pipe, and the straight groove is connected to one end of the left arc groove.

[0011] Furthermore, the detection section includes a right arc groove and an exposure groove, the exposure groove is connected to one end of the right arc groove, and a positioning slot connected to the exposure groove is also provided on the substrate layer.

[0012] Furthermore, a filter assembly is installed in the liquid inlet pipe, and the filter assembly includes an outer shell and a filter membrane. The outer shell is snap-fitted and fixed in the liquid inlet pipe, and the filter membrane is fixedly installed in the outer shell.

[0013] Furthermore, the sewage pipe group includes a positioning block and an external pipe, the positioning block is installed in the positioning slot, and one end of the external pipe is sealed and connected to the positioning block.

[0014] Compared with the prior art, the utility model has the following beneficial effects: the utility model opens a liquid inlet on the substrate layer, and designs the liquid inlet into a structure of a premixing tank and a plurality of diversion pipes, so that the amount of liquid inlet can be flexibly selected as needed when it is convenient to use, and the liquid can be mixed twice by setting the premixing tank and the liquid mixing part, thereby increasing the mixing effect of the liquid. Finally, the liquid outlet is designed into two discharge flow channels, a liquid discharge section and a detection section, so that it can be selectively opened according to the liquid supply needs or the exposure and observation needs when it is easy to use, and has the advantages of flexible use and wide functional applications. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] In order to more clearly illustrate the technical solution of the implementation mode of the utility model, the drawings required for use in the implementation mode will be briefly introduced below. It should be understood that the following drawings only show certain embodiments of the utility model and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other relevant drawings can be obtained based on these drawings without paying creative work.

[0016] Figure 1 It is a three-dimensional diagram of the overall structure in the embodiment of the utility model;

[0017] Figure 2 is a three-dimensional diagram of the substrate layer in the embodiment of the utility model;

[0018] Figure 3 yes Figure 2 a top view of the device shown;

[0019] Figure 4 It is a three-dimensional diagram of the sewage pipe group in the embodiment of the utility model;

[0020] Figure 5 It is a stereoscopic diagram of the filter assembly in the embodiment of the utility model.

[0021] In the figure: 1. substrate layer; 11. positioning slot; 2. cover layer; 21. liquid inlet pipe; 22. liquid discharge pipe; 23. transparent cover; 24. filter assembly; 241. outer shell; 242. filter membrane; 3. liquid inlet section; 31. premixing tank; 32. diversion pipeline; 4. liquid mixing section; 41. lower discharge tank; 42. rising tank; 43. lower curved tank; 5. liquid outlet section; 51. liquid discharge section; 511. left arc tank; 512. straight tank; 52. detection section; 521. right arc tank; 522. exposure tank; 6. sewage pipe group; 61. positioning block; 62. external pipe. DETAILED DESCRIPTION

[0022] In order to make the purpose, technical scheme and advantages of the embodiments of the utility model clearer, the technical scheme in the embodiments of the utility model will be clearly and completely described below in conjunction with the drawings in the embodiments of the utility model. Obviously, the described embodiments are part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model. Therefore, the following detailed description of the embodiments of the utility model provided in the drawings is not intended to limit the scope of the utility model for which protection is claimed, but merely represents selected embodiments of the utility model.

[0023] Reference Figure 1 , Figure 2 and Figure 3As shown, a multifunctional organoid microfluidic chip comprises a substrate layer 1 and a cover layer 2 sealed and mounted on the upper end surface of the substrate layer 1, a liquid inlet portion 3, a liquid mixing portion 4 and a liquid outlet portion 5 are provided on the substrate layer 1, the liquid inlet portion 3, the liquid mixing portion 4 and the liquid outlet portion 5 are sequentially connected, and the liquid outlet portion 5 comprises a liquid discharge section 51 and a detection section 52, the liquid discharge section 51 and the detection section 52 are symmetrically arranged on both sides of the liquid mixing portion 4, and the liquid discharge section 51 and the detection section 52 are both connected to the liquid mixing portion 4, a liquid inlet pipe 21 corresponding to the liquid inlet portion 3, a liquid discharge pipe 22 corresponding to the liquid discharge section 51 and a transparent cover 23 corresponding to the detection section 52 are provided on the cover layer 2, and a sewage discharge pipe group 6 is also installed at the end of the detection section 52. The substrate layer 1 is sealed by arranging a cover layer 2 at the upper end of the substrate layer 1, and the liquid inlet portion 3, the liquid mixing portion 4 and the liquid outlet portion 5 are provided on the substrate layer 1 as flow channels for liquid supply. The liquid outlet portion 5 is designed to be a liquid discharge section 51 and a detection section 52. In the actual use process, the liquid discharged from the liquid mixing portion 4 can be received by the liquid discharge section 51 or the detection section 52, so that the direction of the discharge can be easily selected according to actual needs, thereby meeting different use requirements and increasing the use function of the chip. The liquid inlet pipe 21 is provided on the cover layer 2 to communicate with the liquid inlet portion 3. The heads can correspond to each other, so that the liquid flowing into the liquid inlet pipe 21 can stably enter the liquid inlet part 3. At the same time, the setting of the discharge pipe 22 facilitates the liquid in the discharge section 51 to be stably discharged through the discharge pipe 22. By setting the transparent cover 23 on the detection section 52, it is convenient to observe or expose the liquid in the detection section 52 through the transparent cover 23 during use (for example, chromatographic separation by ultraviolet light) to achieve different usage requirements. The purpose of discharging the liquid in the detection section 52 is achieved by setting the sewage pipe group 6, and it is easy to clean the detection section 52 for use.

[0024] Reference Figure 1 and Figure 5 As shown, a filter assembly 24 is installed in the liquid inlet pipe 21, and the filter assembly 24 includes an outer shell 241 and a filter membrane 242. The outer shell 241 is snap-fitted and fixed in the liquid inlet pipe 21, and the filter membrane 242 is fixedly installed in the outer shell 241. The filter assembly 24 is structurally arranged to achieve installation of the filter membrane 242 through the outer shell 241. When in use, it is installed in the liquid inlet pipe 21 through the outer shell 241, so that the liquid can be filtered through the filter membrane 242, which facilitates better mixing reaction of the liquid.

[0025] Reference Figure 2 and Figure 3As shown, the liquid inlet 3 includes a premixing tank 31 and a plurality of branch pipes 32, and the plurality of branch pipes 32 are evenly arranged on one side of the premixing tank 31, and the branch pipes 32 are connected to the premixing tank 31. By designing the liquid inlet 3 as a structure in which the premixing tank 31 and the plurality of branch pipes 32 cooperate, liquid can be supplied through any two or more branch pipes 32 during use, so that two or more liquids can be conveniently selected to be mixed in the premixing tank 31 according to needs, and the liquid mixed in the premixing tank 31 can be discharged into the liquid mixing part 4.

[0026] Reference Figure 2 and Figure 3 As shown, the liquid mixing part 4 includes a lower row groove 41, an upward groove 42 and a lower curved groove 43, and the lower row groove 41, the upward groove 42 and the lower curved groove 43 are sequentially connected, and the head of the lower row groove 41 is connected to the premixing groove 31, and the lower end of the lower curved groove 43 is connected to the liquid outlet 5. The structure of the liquid mixing part 4 is arranged to facilitate the cooperation of the lower row groove 41, the upward groove 42 and the lower curved groove 43 to achieve repeated movement of the liquid flowing through to achieve more uniform mixing.

[0027] Reference Figure 2 and Figure 3 As shown, the liquid discharge section 51 includes a left arc groove 511 and a straight groove 512 corresponding to the liquid discharge pipe 22, and the straight groove 512 is connected to one end of the left arc groove 511. The structural setting of the liquid discharge section 51 ensures that the liquid is discharged into the liquid discharge pipe 22 through the cooperation of the left arc groove 511 and the straight groove 512. The detection section 52 includes a right arc groove 521 and an exposure groove 522, and the exposure groove 522 is connected to one end of the right arc groove 521, and a positioning card groove 11 connected to the exposure groove 522 is also provided on the substrate layer 1. The structural setting of the detection section 52 facilitates the temporary storage of the solution in the exposure groove 522 through the right arc groove 521 when the liquid needs to be observed or processed.

[0028] Reference Figure 4 As shown, the sewage pipe group 6 includes a positioning block 61 and an external pipe 62. The positioning block 61 is installed in the positioning slot 11, and one end of the external pipe 62 is sealed and connected to the positioning block 61. The structural setting of the sewage pipe group 6 ensures that when in use, it can be installed in the positioning slot 11 through the positioning block 61, and then connected to the external waste liquid pool through the external pipe 62, so that it is easy to better process the liquid in the detection section 52.

[0029] Working principle: In actual use, the number of liquid inlet pipes 21 to be opened can be selected according to needs. For example, if two liquids need to be mixed, only two liquid inlet pipes 21 need to be opened, and the other liquid inlet pipes 21 are all closed. The liquid after entering is converged into the premixing tank 31 from each branch pipe 32 for preliminary mixing, and then flows through the mixing part 4 for mixing again, so as to achieve a more uniform mixing effect. The mixed liquid can then enter the liquid outlet part 5. When the liquid needs to flow into the discharge section 51, the discharge pipe 22 on the cover layer 2 needs to be opened, and the external pipe 62 needs to be closed. When the liquid needs to flow into the detection section 52, the discharge pipe 22 needs to be closed, and the external pipe 62 needs to be opened. In this way, after the liquid is gathered in the exposure tank 522, it can be exposed or observed from the outside.

[0030] The above description is only the preferred implementation of the utility model, and is not intended to limit the utility model. For those skilled in the art, the utility model can have various modifications and changes. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the utility model shall be included in the protection scope of the utility model.

Claims

1. A multifunctional organoid microfluidic chip, comprising a substrate layer (1) and a cover layer (2) sealed and mounted on the upper end surface of the substrate layer (1), characterized in that: The substrate layer (1) is provided with a liquid inlet portion (3), a liquid mixing portion (4) and a liquid outlet portion (5); the liquid inlet portion (3), the liquid mixing portion (4) and the liquid outlet portion (5) are sequentially connected, and the liquid outlet portion (5) comprises a liquid discharge section (51) and a detection section (52); the liquid discharge section (51) and the detection section (52) are symmetrically arranged on both sides of the liquid mixing portion (4), and both the liquid discharge section (51) and the detection section (52) are connected to the liquid mixing portion (4); the cover layer (2) is provided with a liquid inlet pipe (21) corresponding to the liquid inlet portion (3), a liquid discharge pipe (22) corresponding to the liquid discharge section (51) and a transparent cover (23) corresponding to the detection section (52); and a sewage discharge pipe group (6) is also installed at the end of the detection section (52).

2. The multifunctional organoid microfluidic chip according to claim 1, characterized in that: The liquid inlet portion (3) comprises a premixing tank (31) and a plurality of diversion pipes (32). The plurality of diversion pipes (32) are evenly arranged on one side of the premixing tank (31), and the diversion pipes (32) are connected to the premixing tank (31).

3. The multifunctional organoid microfluidic chip according to claim 2, characterized in that: The liquid mixing portion (4) comprises a lower row of grooves (41), an upward groove (42) and a lower curved groove (43); the lower row of grooves (41), the upward groove (42) and the lower curved groove (43) are sequentially connected, and the head of the lower row of grooves (41) is connected to the premixing groove (31), and the lower end of the lower curved groove (43) is connected to the liquid outlet portion (5).

4. The multifunctional organoid microfluidic chip according to claim 3, characterized in that: The liquid discharge section (51) comprises a left arc groove (511) and a straight groove (512) corresponding to the liquid discharge pipe (22), wherein the straight groove (512) is connected to one end of the left arc groove (511).

5. The multifunctional organoid microfluidic chip according to claim 4, characterized in that: The detection section (52) comprises a right arc groove (521) and an exposure groove (522), wherein the exposure groove (522) is connected to one end of the right arc groove (521), and a positioning card groove (11) connected to the exposure groove (522) is also provided on the substrate layer (1).

6. The multifunctional organoid microfluidic chip according to claim 5, characterized in that: A filter assembly (24) is installed in the liquid inlet pipe (21), and the filter assembly (24) comprises an outer shell (241) and a filter membrane (242). The outer shell (241) is snap-fitted and fixed in the liquid inlet pipe (21), and the filter membrane (242) is fixedly installed in the outer shell (241).

7. The multifunctional organoid microfluidic chip according to claim 6, characterized in that: The sewage pipe group (6) comprises a positioning block (61) and an external pipe (62); the positioning block (61) is installed in the positioning slot (11); one end of the external pipe (62) is sealed and connected to the positioning block (61).

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