Household portable nucleic acid extraction device

Abstract

The utility model discloses a household portable nucleic acid extraction device which is characterized by comprising a self-heating pipe, a nucleic acid extraction pipe, a magnetic bead pre-assembling module, a stirring piece, a lysis solution pre-assembling module, a sealing cover, a sampling rod and a magnetic rod, a self-heating structure is arranged in the self-heating pipe, a sealing film easy to damage is arranged at a pipe opening of the self-heating pipe, and the outer diameter of the nucleic acid extraction pipe is smaller than the inner diameter of the self-heating pipe; the magnetic bead pre-assembling module, the stirring piece and the lysis solution pre-assembling module are placed in the nucleic acid extraction tube, and a sealing cover covers a tube opening of the nucleic acid extraction tube; the sampling rod sequentially penetrates through the sealing cover, the lysate pre-assembling module, the stirring part and the magnetic bead pre-assembling module, and the periphery of the lower part of the sampling rod is provided with a spiral structure for driving the stirring part to rotate; the magnetic rod extends into the nucleic acid extraction tube from the axial through hole of the sampling rod and is used for adsorbing the magnetic beads. The nucleic acid extraction device has the beneficial effects that the structure is simple, the operation is convenient, the nucleic acid extraction device is suitable for extracting nucleic acid under a non-experimental environment condition, and the nucleic acid extraction difficulty is reduced.

Description

Technical Field

[0001] The utility model relates to the field of medical instruments, and in particular to a portable nucleic acid extraction device for home use. Background Art

[0002] In nucleic acid detection tests, obtaining high-purity nucleic acids is one of the most important steps, and is closely related to subsequent application analyses such as gene amplification, sequencing, hybridization, and cloning. Commonly used microbial nucleic acid extraction techniques include phenol-chloroform method, salting-out method, silica phase adsorption method, ion exchange method, etc. These traditional nucleic acid extraction methods have shortcomings such as long time consumption, low extraction efficiency, and cumbersome labor operations, which greatly limit the work efficiency of operators and the quality of nucleic acid products. In addition, these extraction techniques require frequent centrifugation steps and professional technicians, and are not suitable for extraction in non-laboratory environments. Summary of the invention

[0003] In view of the deficiencies in the above-mentioned prior art, the utility model provides a portable nucleic acid extraction device for home use, which does not rely on laboratory equipment and can realize nucleic acid extraction in a non-laboratory environment.

[0004] In order to achieve the above purpose, the utility model adopts the following technical solutions:

[0005] A portable nucleic acid extraction device for home use, characterized in that it comprises a self-heating tube, a nucleic acid extraction tube, a magnetic bead pre-installed module, a stirring element, a lysate pre-installed module, a sealing cover, a sampling rod, and a magnetic rod;

[0006] The self-heating tube has a self-heating structure inside, and the tube mouth of the self-heating tube is provided with an easily-destructible sealing film. The outer diameter of the nucleic acid extraction tube is smaller than the inner diameter of the self-heating tube, so that the nucleic acid extraction tube can be loaded into the self-heating tube;

[0007] The magnetic bead pre-installed module, the stirring element, and the lysate pre-installed module are placed in the nucleic acid extraction tube in sequence from bottom to top, and a sealing cover is provided at the tube opening of the nucleic acid extraction tube;

[0008] The sampling rod passes through the sealing cover, the lysate pre-installed module, the stirring element, and the magnetic bead pre-installed module in sequence, and releases the lysate in the lysate pre-installed module and the magnetic bead mixture in the magnetic bead pre-installed module.

[0009] The sampling rod has a through hole along the axis, and the outer periphery of the lower part of the sampling rod has a spiral structure for driving the stirring element to rotate;

[0010] The magnetic rod extends from the axial through hole of the sampling rod into the nucleic acid extraction tube for adsorbing magnetic beads.

[0011] Furthermore, the self-heating structure is evenly distributed on the inner wall of the self-heating pipe, and when the sealing film at the pipe opening of the self-heating pipe is damaged, the self-heating structure contacts the air and starts to generate heat.

[0012] Furthermore, the inner bottom of the self-heating tube has a conical ring-mounted positioning structure in the middle, and the bottom of the nucleic acid extraction tube has an inverted cone structure adapted to the conical ring-mounted positioning structure, so that the nucleic acid extraction tube is positioned on the central axis of the self-heating tube and maintains a predetermined gap with the inner wall of the self-heating tube.

[0013] Furthermore, the upper end of the sampling rod has a flange structure, and a spring is sleeved on the upper part of the sampling rod, and both ends of the spring are respectively limited between the flange structure and the top surface of the sealing cover.

[0014] Furthermore, the magnetic bead pre-loading module comprises a first outer ring, the upper and lower ends of the first outer ring are sealed by easily destructible films, and the magnetic bead mixture is pre-loaded into the inner cavity of the first outer ring.

[0015] Furthermore, the inner side wall of the first outer ring is provided with a plurality of first guide parts cooperating with the spiral structure, so that the first outer ring can rotate along with the up and down movement of the sampling rod.

[0016] Furthermore, the lysis solution pre-loading module comprises a second outer ring, the upper and lower ends of the second outer ring are sealed by easily destructible films, and the lysis solution is pre-loaded into the inner cavity of the second outer ring.

[0017] Furthermore, the inner side wall of the second outer ring is provided with a plurality of second guide parts cooperating with the spiral structure, so that the second outer ring rotates as the sampling rod moves up and down.

[0018] Furthermore, stirring blades are distributed on the periphery of the stirring member, and a guide portion cooperating with the spiral structure is provided in the shaft hole of the stirring member, so that the stirring member rotates when the sampling rod moves up and down.

[0019] The beneficial effects of the utility model include: simple structure, convenient operation, and suitability for extracting nucleic acid under non-experimental environmental conditions, thereby reducing the difficulty of nucleic acid extraction for ordinary people. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 It is a schematic diagram of the explosion structure of the utility model;

[0021] Figure 2 It is a cross-sectional view of the utility model. DETAILED DESCRIPTION

[0022] The present invention will be further described in detail below in conjunction with specific embodiments and accompanying drawings.

[0023] A kind of Figure 1-2 The portable home nucleic acid extraction device shown includes a self-heating tube 1, a nucleic acid extraction tube 2, a magnetic bead pre-installed module 3, a stirring element 4, a lysis solution pre-installed module 5, a sealing cover 6, a sampling rod 7, and a magnetic rod 8; wherein the self-heating tube 1 has a self-heating structure inside, and the tube mouth of the self-heating tube 1 is provided with an easily destructible sealing film, which is an aluminum seal, and the self-heating structure is evenly distributed on the inner wall of the self-heating tube 1, and when the sealing film at the tube mouth of the self-heating tube 1 is destroyed, the self-heating structure is an oxidative heating mixed material, which starts to heat up when it comes into contact with the air, and its working principle is the same as that of the common hand warming patch on the market.

[0024] The outer diameter of the nucleic acid extraction tube 2 is smaller than the inner diameter of the self-heating tube 1, so that the nucleic acid extraction tube 2 can be loaded into the self-heating tube 1; Figure 2 The self-heating tube 1 shown has a conical ring-mounted positioning structure 11 in the middle of the inner bottom, and the bottom of the nucleic acid extraction tube 2 has an inverted cone structure 21 adapted to the conical ring-mounted positioning structure 11, so that the nucleic acid extraction tube 2 is positioned on the central axis of the self-heating tube 1 and maintains a predetermined gap with the inner wall of the self-heating tube 1, thereby ensuring that the nucleic acid extraction tube 2 is heated evenly.

[0025] The magnetic bead pre-loading module 3, the stirring element 4, and the lysate pre-loading module 5 are sequentially placed in the nucleic acid extraction tube 2 from bottom to top, and a sealing cover 6 is provided at the tube mouth of the nucleic acid extraction tube 2; the magnetic bead pre-loading module 3, the stirring element 4, and the lysate pre-loading module 5 leave a movable gap in the nucleic acid extraction tube 2. The magnetic bead pre-loading module 3 includes a first outer ring 31, and the upper and lower ends of the first outer ring 31 are sealed by an easily destructible film, and the magnetic bead mixture is pre-loaded into the inner cavity of the first outer ring 31. Similarly, the lysate pre-loading module 5 includes a second outer ring 51, and the upper and lower ends of the second outer ring 51 are sealed by an easily destructible film, and the lysate is pre-loaded into the inner cavity of the second outer ring 51.

[0026] When in use, the sampling rod 7 passes through the sealing cover 6, the lysate pre-loading module 5, the stirring member 4, and the magnetic bead pre-loading module 3 in sequence, and releases the lysate in the lysate pre-loading module 5 and the magnetic bead mixture in the magnetic bead pre-loading module 3. The sampling rod 7 has a through hole along the axis, and the lower periphery of the sampling rod 7 has a spiral structure 71 for driving the stirring member 4 to rotate; the inner side wall of the first outer ring 31 has a plurality of first guide portions 32 that cooperate with the spiral structure 71, so that the first outer ring 31 can rotate as the sampling rod 7 moves up and down. Furthermore, stirring blades 41 are distributed on the outer periphery of the stirring member 4, and the axial hole of the stirring member 4 has a guide portion that cooperates with the spiral structure 71, so that the stirring member 4 rotates when the sampling rod 7 moves up and down. The inner side wall of the second outer ring 51 has a plurality of second guide portions 52 that cooperate with the spiral structure 71, so that the second outer ring 51 rotates as the sampling rod 7 moves up and down. Therefore, when the sampling rod 7 moves up and down, the first outer ring 31, the stirring member 4, and the second outer ring 51 all rotate, so that the liquid in the nucleic acid extraction tube 2 is fully stirred and mixed.

[0027] To facilitate the sampling rod 7 to move up and down by pressing, the upper end of the sampling rod 7 of this embodiment has a flange structure 72, and a spring 10 is sleeved on the upper part of the sampling rod 7, and the two ends of the spring 10 are respectively limited between the flange structure 72 and the top surface of the sealing cover 6. Therefore, after each pressing of the sampling rod 7, the sampling rod 7 can automatically bounce up with the elastic force of the spring 10, which is convenient for quick operation.

[0028] The magnetic rod 8 extends from the axial through hole of the sampling rod 7 into the nucleic acid extraction tube 2 for extracting the amplification product.

[0029] The specific operation method of the utility model is as follows:

[0030] 1. Tear open the aluminum seal of the self-heating tube, so that its inside is in contact with the air and starts to heat up;

[0031] 2. Sampling: Take an appropriate amount of sample (rice grain size) with the tip of the sampling stick and place it into the nucleic acid extraction tube by rotating it. (If the specimen is liquid, use the matching pipette to absorb about 200 microliters of the liquid to be tested) Tighten the lid, then put the nucleic acid extraction tube into the self-heating tube, press the top of the sampling stick about 50 times for about 5 minutes to allow the nucleic acid sample to be fully adsorbed onto the magnetic beads;

[0032] 3. Magnetic absorption: Insert the magnetic bar into the nucleic acid extraction tube from the center hole of the sampling rod and let it stand for 1 minute so that all the magnetic beads are adsorbed to the bottom of the magnetic bar;

[0033] 4. Take out the sampling stick together with the magnetic stick, and then wash, elute and other operations to obtain the amplified product.

[0034] The technical solutions provided by the embodiments of the present invention are introduced in detail above. Specific examples are used in this article to illustrate the principles and implementation methods of the embodiments of the present invention. The description of the above embodiments is only applicable to help understand the principles of the embodiments of the present invention. At the same time, for those skilled in the art, according to the embodiments of the present invention, there will be changes in the specific implementation methods and application scopes. In summary, the content of this specification should not be understood as a limitation on the present invention.

Claims

1. A portable nucleic acid extraction device for home use, characterized in that: It comprises a self-heating tube (1), a nucleic acid extraction tube (2), a magnetic bead pre-installed module (3), a stirring element (4), a lysate pre-installed module (5), a sealing cover (6), a sampling rod (7), and a magnetic rod (8); The self-heating tube (1) has a self-heating structure inside, the tube mouth of the self-heating tube (1) is provided with an easily-destructible sealing film, the outer diameter of the nucleic acid extraction tube (2) is smaller than the inner diameter of the self-heating tube (1), so that the nucleic acid extraction tube (2) can be loaded into the self-heating tube (1); The magnetic bead pre-installed module (3), the stirring element (4), and the lysate pre-installed module (5) are sequentially placed in the nucleic acid extraction tube (2) from bottom to top, and a sealing cover (6) is provided at the tube mouth of the nucleic acid extraction tube (2); The sampling rod (7) passes through the sealing cover (6), the lysate pre-loading module (5), the stirring element (4), and the magnetic bead pre-loading module (3) in sequence, and releases the lysate in the lysate pre-loading module (5) and the magnetic bead mixture in the magnetic bead pre-loading module (3). The sampling rod (7) has a through hole along the axis, and the outer periphery of the lower part of the sampling rod (7) has a spiral structure (71) for driving the stirring member (4) to rotate; The magnetic rod (8) extends from the axial through hole of the sampling rod (7) into the nucleic acid extraction tube (2) and is used to absorb magnetic beads.

2. A portable nucleic acid extraction device for home use according to claim 1, characterized in that: The self-heating structure is evenly distributed on the inner wall of the self-heating pipe (1), and when the sealing film at the pipe opening of the self-heating pipe (1) is damaged, the self-heating structure comes into contact with the air and starts to generate heat.

3. A portable nucleic acid extraction device for home use according to claim 1, characterized in that: The inner bottom of the self-heating tube (1) has a conical ring-mounted positioning structure (11) in the middle, and the bottom of the nucleic acid extraction tube (2) has an inverted cone structure (21) adapted to the conical ring-mounted positioning structure (11), so that the nucleic acid extraction tube (2) is positioned on the central axis of the self-heating tube (1) and maintains a predetermined gap with the inner wall of the self-heating tube (1).

4. A portable nucleic acid extraction device for home use according to claim 1, characterized in that: The upper end of the sampling rod (7) has a flange structure (72), and the upper part of the sampling rod (7) is sleeved with a spring (10), and the two ends of the spring (10) are respectively limited between the flange structure (72) and the top surface of the sealing cover (6).

5. A portable nucleic acid extraction device for home use according to claim 1, characterized in that: The magnetic bead pre-installed module (3) comprises a first outer ring (31), the upper and lower ends of the first outer ring (31) are sealed by easily destructible films, and the magnetic bead mixture is pre-installed in the inner cavity of the first outer ring (31).

6. A portable nucleic acid extraction device for home use according to claim 5, characterized in that: The inner side wall of the first outer ring (31) is provided with a plurality of first guide portions (32) cooperating with the spiral structure (71), so that the first outer ring (31) can rotate along with the up and down movement of the sampling rod (7).

7. A portable nucleic acid extraction device for home use according to claim 1, characterized in that: The lysis solution pre-loading module (5) comprises a second outer ring (51), the upper and lower ends of the second outer ring (51) are sealed by an easily destructible film, and the lysis solution is pre-loaded into the inner cavity of the second outer ring (51).

8. A portable nucleic acid extraction device for home use according to claim 7, characterized in that: The inner side wall of the second outer ring (51) is provided with a plurality of second guide portions (52) cooperating with the spiral structure (71), so that the second outer ring (51) rotates as the sampling rod (7) moves up and down.

9. A portable nucleic acid extraction device for home use according to claim 1, characterized in that: Stirring blades (41) are distributed on the periphery of the stirring member (4), and a guide portion cooperating with the spiral structure (71) is provided in the axial hole of the stirring member (4), so that the stirring member (4) rotates when the sampling rod (7) moves up and down.

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