Escherichia coli separation and storage device
By designing an E. coli isolation and storage device including a base plate, a stent, a tube centrifuge, a storage box, a clear liquid bottle and a turbid liquid bottle, the problem of E. coli waste caused by the adhesion of residual liquid in the inner wall of the catheter is solved, and effective low-temperature preservation and protection of E. coli quantity are achieved.
Patent Information
- Application Number
- CN202420556274.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-03-21
- Publication Date
- 2025-03-14
- Estimated Expiration
- 2034-03-21
AI Technical Summary
During the separation process of the existing E. coli isolation device, the inner wall of the catheter is prone to adhere to a large amount of residual liquid, resulting in wasting the amount of E. coli.
An E. coli isolation storage device is designed, including a base plate, a stent, a tube centrifuge, a storage box, a clear liquid bottle and a turbid liquid bottle. Coolant is input through the water inlet pipe, fill the surroundings of the clean liquid bottle in the storage box, reflux the residual liquid by gravity, and through the cooperation of the cap and damping slider, prevent external air from contact and reduce solution waste.
It effectively reduces the adhesion of residual fluid in the inner wall of the catheter, reduces the waste of E. coli, improves the survival rate during the isolation process, and extends the effective survival time of E. coli through low temperature preservation.
Smart Images
Figure CN222613324U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of Escherichia coli separation and storage devices. Background Art
[0002] Enterobacteriaceae are normal parasites in the intestines of animals, and a very small portion of them can cause disease under certain conditions. The serotypes of Escherichia coli can cause gastrointestinal infections in humans or animals, which are mainly caused by specific fimbriae antigens, pathogenic toxins, etc. In addition to gastrointestinal infections, they can also cause urinary tract infections, arthritis, meningitis, and septicemic infections.
[0003] At present, a tubular centrifuge is used for separating E. coli. During operation, the staff generally directly uses a container to catch the separated E. coli. However, during a period of separation, not only will the separated E. coli have more contact with the outside air, but it is also easily affected by the outside temperature, which may lead to a low survival rate of the separated E. coli. Therefore, the existing device directly connects to the liquid outlet through a catheter, thereby reducing the contact time between E. coli and the outside air. However, a large amount of residual liquid will adhere to the inner wall of the catheter, thus resulting in a waste of E. coli. Therefore, it is necessary to reduce the amount of residual liquid adhering to the inner wall of the catheter. Utility Model Content
[0004] The utility model aims to overcome the defect that a large amount of residual liquid remains on the inner wall of the catheter and provides an Escherichia coli separation and storage device.
[0005] The technical solution to achieve the above-mentioned purpose is: an Escherichia coli separation and storage device, comprising a bottom plate, a bracket connected to the top of the bottom plate, a tubular centrifuge connected to the outer wall of the bracket, a storage box connected to the top of the bottom plate, a first partition connected to the inner wall of the storage box, a second partition connected to the outer wall of the first partition, a clear liquid bottle placed in the storage box, a turbid liquid bottle placed in the storage box, a through hole provided at the bottom end of the outer wall of the first partition, a bottom tube threadedly connected at the top opening of the clear liquid bottle and the top opening of the turbid liquid bottle, a hose connected to the top of the bottom tube, a receiving tube connected to the top of the hose, a damping slider slidably connected to the outer wall of the receiving tube, a sealing cap rotatably connected to the top of the receiving tube, a slot provided on the outer wall of the sealing cap, a limiting member connected to the outer wall of the bracket, and the outer wall of the bottom tube passing through the top of the storage box.
[0006] Preferably, the first partition divides the inner wall of the storage box into a first inner cavity and a second inner cavity, and the second partition divides the second inner cavity into a first placement area and a second placement area, wherein a clear liquid bottle is placed in the first placement area and a turbid liquid bottle is placed in the second placement area.
[0007] Preferably, the top end of the storage box is arranged as a square hole, the upper end of the square hole is connected with a top cover inserted therein, and the outer wall of the bottom tube passes through the top cover.
[0008] Preferably, the receiving tube is truncated cone-shaped.
[0009] Preferably, the limiting member comprises a limiting plate and a magnet, the outer wall of the bracket is rotatably connected to two symmetrically distributed limiting plates, the outer wall of the bracket is connected to symmetrically distributed magnets, and the limiting plates are made of metal iron plates.
[0010] Preferably, the outer wall of the bracket and the outer wall of the limiting plate are both provided with arc grooves.
[0011] Preferably, the limiting plate is located directly above the bottom tube.
[0012] Preferably, a water inlet pipe is connected to the top of the outer wall of the storage box, a push rod is threadedly connected to the top of the storage box, a push plate is rotatably connected to the bottom end of the push rod, and the push plate is located in the first interval.
[0013] The beneficial effects of the utility model are as follows: cold cooling liquid is input into the first interval through the water inlet pipe, the water inlet pipe is closed, and then the push rod is pressed downward to make the push plate press the cooling liquid into the first placement area through the through hole, and the area around the clear liquid bottle is filled with cooling liquid to store Escherichia coli at low temperatures, and the hose is pulled up vertically to make the receiving tube pass over the top of the limiting member, and the limiting member clamps the receiving tube on the top, thereby keeping the hose in a vertical state, so that the residual liquid in the hose can rely on its own gravity to flow back into the clear liquid bottle, and at the same time, the cover is rotated to close the receiving tube, the card slot is aligned with the damping slider, the damping slider is inserted into the card slot, and the cover is fixed to prevent outside air from contacting the solution in the hose and the clear liquid bottle, thereby reducing the waste of centrifugal solution. BRIEF DESCRIPTION OF THE DRAWINGS
[0014] Figure 1 It is a three-dimensional structural schematic diagram of the utility model;
[0015] Figure 2 It is a schematic diagram of the top view structure of the utility model;
[0016] Figure 3 It is a schematic diagram of the cross-sectional structure of the utility model;
[0017] Figure 4 yes Figure 2 A schematic diagram of the enlarged structure at A in the middle;
[0018] Figure 5 yes Figure 3 Schematic diagram of the enlarged structure at point B in the middle.
[0019] 1. Bottom plate; 2. Bracket; 3. Tubular centrifuge; 4. Storage box; 5. First partition plate; 6. Second partition plate; 7. Clear liquid bottle; 8. Turbid liquid bottle; 9. Through hole; 10. Push rod; 11. Push plate; 12. Bottom tube; 13. Hose; 14. Receiving tube; 15. Damping slider; 16. Cover; 17. Slot; 18. Limit plate; 19. Magnet; 20. Water inlet pipe. DETAILED DESCRIPTION
[0020] The technical solution of the present invention will be described clearly and completely below in conjunction with the accompanying drawings. In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inside", "outside" and the like indicate directions or positional relationships based on the directions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and therefore cannot be understood as a limitation on the present invention. In addition, the terms "first", "second", and "third" are used for descriptive purposes only, and cannot be understood as indicating or implying relative importance.
[0021] The utility model will be further described below in conjunction with the accompanying drawings.
[0022] Reference Figure 1-5 A device for separating and storing Escherichia coli comprises a bottom plate 1, a bracket 2 is connected to the top of the bottom plate 1, a tubular centrifuge 3 is connected to the outer wall of the bracket 2, a storage box 4 is connected to the top of the bottom plate 1, a square hole is arranged at the top of the storage box 4, a top cover is inserted and connected to the upper end of the square hole, an outer wall of a bottom tube 12 passes through the top cover, a first partition 5 is connected to the inner wall of the storage box 4, a second partition 6 is connected to the outer wall of the first partition 5, a clear liquid bottle 7 is placed in the storage box 4, a turbid liquid bottle 8 is placed in the storage box 4, a through hole 9 is opened at the bottom end of the outer wall of the first partition 5, a bottom tube 12 is threadedly connected to the top opening of the clear liquid bottle 7 and the top opening of the turbid liquid bottle 8, a hose 13 is connected to the top of the bottom tube 12, and a receiving tube 14 is connected to the top of the hose 13 The receiving tube 14 is in the shape of a truncated cone, and a damping slider 15 is slidably connected to the outer wall of the receiving tube 14, and a cover 16 is rotatably connected to the top of the receiving tube 14, and a slot 17 is provided on the outer wall of the cover 16. The outer wall of the bracket 2 is connected to a limiting member, and the outer wall of the bottom tube 12 passes through the top of the storage box 4. The first partition 5 divides the inner wall of the storage box 4 into a first inner cavity and a second inner cavity, and the second partition 6 divides the second inner cavity into a first placement area and a second placement area. A clear liquid bottle 7 is placed in the first placement area, and a turbid liquid bottle 8 is placed in the second placement area. The top of the outer wall of the storage box 4 is connected to a water inlet pipe 20, and the top of the storage box 4 is threadedly connected to a push rod 10, and the bottom end of the push rod 10 is rotatably connected to a push plate 11, and the push plate 11 is located in the first interval.
[0023] The E. coli solution is separated by using the tubular centrifuge 3, and the receiving tubes 14 respectively associated with the clear liquid bottle 7 and the turbid liquid bottle 8 are inserted into the corresponding discharge pipe ports of the tubular centrifuge 3 to reduce the contact time between the solution and the outside world. The clear liquid and the turbid liquid flow into the hose 13 through the corresponding receiving tubes 14, and then flow to the bottom tube 12 through the hose 13, and finally flow into the clear liquid bottle 7 and the turbid liquid bottle 8 respectively. At this time, the push rod 10 is rotated to move the push rod 10 upward, and the push plate 11 is pulled to the top, and the cold coolant is input into the first interval through the water inlet pipe 20, and the water inlet pipe 20 is closed, and then the push rod 10 is squeezed downward to make the push plate 11 pass the coolant through The through hole 9 is pressed into the first placement area, and the area around the clear liquid bottle 7 is filled with coolant to preserve the Escherichia coli at low temperatures. The hose 13 is pulled up vertically to allow the receiving tube 14 to pass over the top of the limiter. The limiter clamps the receiving tube 14 on the top, thereby maintaining the vertical state of the hose 13, so that the residual liquid in the hose 13 can rely on its own gravity to flow back into the clear liquid bottle 7. At the same time, the cover 16 is rotated to close the receiving tube 14, the slot 17 is aligned with the damping slider 15, the damping slider 15 is inserted into the slot 17, and the cover 16 is fixed to prevent outside air from contacting the solution in the hose 13 and the clear liquid bottle 7, thereby reducing the waste of centrifugal solution.
[0024] Reference Figure 4 The limiting member includes a limiting plate 18 and a magnet 19. The outer wall of the bracket 2 is rotatably connected to two symmetrically distributed limiting plates 18. The outer wall of the bracket 2 is connected to symmetrically distributed magnets 19. The limiting plate 18 is made of metal iron plate. The outer wall of the bracket 2 and the outer wall of the limiting plate 18 are both provided with arc grooves. The limiting plate 18 is located directly above the bottom tube 12.
[0025] Pull up the hose 13 vertically to make the receiving tube 14 pass over the limit plate 18, rotate the limit plate 18 to make the limit plate 18 contact the magnet 19, and make the receiving tube 14 stuck in the gap between the limit plate 18 and the outer wall of the bracket 2 to fix the position of the receiving tube 14. The limit plate 18 is made of metal iron plate, and the magnet 19 attracts the limit plate 18 to fix the limit plate 18.
[0026] The above embodiments are only used to illustrate the technical solution of the utility model, rather than to limit it. Although the utility model is described in detail with reference to the above embodiments, ordinary technicians in this field should understand that they can still modify the technical solutions recorded in the above embodiments, or replace some or all of the technical features therein with equivalents. These modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the utility model.
Claims
1. An Escherichia coli separation and storage device, comprising a bottom plate (1), the top of the bottom plate (1) is connected to a bracket (2), the outer wall of the bracket (2) is connected to a tubular centrifuge (3), characterized in that: The top end of the bottom plate (1) is connected to a storage box (4), the inner wall of the storage box (4) is connected to a first partition (5), the outer wall of the first partition (5) is connected to a second partition (6), a clear liquid bottle (7) is placed in the storage box (4), a turbid liquid bottle (8) is placed in the storage box (4), a through hole (9) is opened at the bottom end of the outer wall of the first partition (5), and the top openings of the clear liquid bottle (7) and the turbid liquid bottle (8) are both threadedly connected. A bottom tube (12), the top end of the bottom tube (12) is connected to a hose (13), the top end of the hose (13) is connected to a receiving tube (14), the outer wall of the receiving tube (14) is slidably connected to a damping slider (15), the top end of the receiving tube (14) is rotatably connected to a cover (16), the outer wall of the cover (16) is provided with a slot (17), the outer wall of the bracket (2) is connected to a limiting member, and the outer wall of the bottom tube (12) passes through the top end of the storage box (4).
2. The E. coli separation and storage device according to claim 1, characterized in that: The first partition (5) divides the inner wall of the storage box (4) into a first inner cavity and a second inner cavity, and the second partition (6) divides the second inner cavity into a first placement area and a second placement area. A clear liquid bottle (7) is placed in the first placement area, and a turbid liquid bottle (8) is placed in the second placement area.
3. The E. coli separation and storage device according to claim 1, characterized in that: The top end of the storage box (4) is arranged as a square hole, a top cover is inserted and connected to the upper end of the square hole, and the outer wall of the bottom tube (12) passes through the top cover.
4. The E. coli separation and storage device according to claim 1, characterized in that: The receiving tube (14) is in the shape of a truncated cone.
5. The E. coli separation and storage device according to claim 1, characterized in that: The limiting member comprises a limiting plate (18) and a magnet (19); the outer wall of the bracket (2) is rotatably connected to two symmetrically distributed limiting plates (18); the outer wall of the bracket (2) is connected to symmetrically distributed magnets (19); and the limiting plate (18) is made of metal iron plate.
6. The E. coli separation and storage device according to claim 5, characterized in that: The outer wall of the bracket (2) and the outer wall of the limiting plate (18) are both provided with arc grooves.
7. The E. coli separation and storage device according to claim 5, characterized in that: The limiting plate (18) is located directly above the bottom tube (12).
8. The E. coli separation and storage device according to claim 2, characterized in that: The top end of the outer wall of the storage box (4) is connected to a water inlet pipe (20), the top end of the storage box (4) is threadedly connected to a push rod (10), the bottom end of the push rod (10) is rotatably connected to a push plate (11), and the push plate (11) is located in the first interval.