Vector and PCR (Polymerase Chain Reaction) detection system

Abstract

The utility model relates to a carrier and PCR (Polymerase Chain Reaction) detection system, the carrier comprises a cavity wall, the cavity wall comprises a first wall, a second wall and a side wall, the first wall and the second wall are oppositely arranged, the side wall is arranged between the first wall and the second wall, the side wall is annular, a reaction cavity for accommodating reaction liquid is defined by the cavity wall, and at least part of the cavity wall can be heated by an electromagnetic field. The PCR detection system comprises an electromagnetic heating mechanism and a cooling mechanism, the electromagnetic heating mechanism is used for heating the carrier, and the cooling mechanism is used for cooling the carrier.

Description

Technical Field

[0001] The utility model relates to the technical field of in vitro diagnosis, in particular to a carrier and a PCR detection system. Background Art

[0002] PCR (polymerase chain reaction) refers to a molecular biology experimental method for in vitro enzymatic synthesis of specific DNA fragments, which is mainly composed of repeated thermal cycles of three steps: high temperature denaturation, low temperature annealing and suitable temperature extension. Before the PCR reaction, the reaction solution needs to be placed in a carrier, where the reaction solution is composed of samples such as collected throat swabs or nasal swabs and reagents for PCR reactions. During the PCR reaction, the reaction solution needs to be heated or cooled by the temperature module of the PCR instrument, so that the reaction solution circulates in the high temperature denaturation, low temperature annealing and suitable temperature extension stages.

[0003] The existing technology has a long detection time and a slow heating and cooling speed, which is mainly caused by factors such as the large thermal capacity of the heater, the large thermal resistance between the heater and the carrier, and the slow thermal balance of the carrier.

[0004] The existing technology uses contact heating, which easily generates condensed water during the heating and cooling process. The condensed water can cause circuit short circuits and reduce the reliability of the equipment.

[0005] The existing temperature sensing technology directly detects the temperature of the reaction liquid or the carrier, which is easy to cause corrosion of the temperature sensor and affect the accuracy of the detection.

[0006] In the prior art, the content of the object to be detected is often determined by fluorescence detection. At the same time, the prior art discloses the method of determining the temperature of a solid object by fluorescence detection. However, when the temperature detection method is applied to the detection of the content of the fluorescent detection component, it is inevitable that the fluorescence of the temperature detection and the fluorescence of the component content detection interfere with each other, resulting in poor detection accuracy. In addition, the temperature detection method by fluorescence in the prior art has low sensitivity. Utility Model Content

[0007] One purpose of the utility model is to provide a carrier to at least solve one of the above technical problems.

[0008] To achieve the above-mentioned purpose, the first aspect of the utility model provides a carrier, including a cavity wall, wherein the cavity wall includes a first wall and a second wall arranged opposite to each other and a side wall arranged between the first wall and the second wall, wherein the side wall is annular, and the cavity wall encloses a reaction cavity for accommodating a reaction liquid, and at least a portion of the cavity wall can be heated by an electromagnetic field.

[0009] Optionally, the carrier further includes a temperature sensing unit, and the temperature sensing unit is used to reflect the temperature of the reaction liquid.

[0010] Optionally, the temperature sensing portion includes a first fluorescent temperature-sensitive portion disposed on the outer side of the cavity wall, the first fluorescent temperature-sensitive portion includes a fluorescent temperature-sensitive material, and the first fluorescent temperature-sensitive portion can reflect the temperature of the reaction liquid.

[0011] Optionally, a light-isolating portion is further provided between the first fluorescent temperature-sensitive portion and the cavity wall.

[0012] Optionally, the temperature sensing portion is arranged on the outer side of the first wall, and at least a portion of the second wall or at least a portion of the side wall is made of a transparent material.

[0013] Optionally, at least part of the cavity wall is formed by a heat conductive sheet, and the heat conductive sheet can be heated by an electromagnetic field.

[0014] Optionally, the heat conductive sheet is a metal sheet.

[0015] Optionally, the thickness of the thermal conductive sheet is 10-100 μm.

[0016] Optionally, at least part of the cavity wall is made of transparent material.

[0017] Optionally, the transparent material is polydimethylsiloxane, polypropylene, organic glass or polycarbonate.

[0018] Optionally, the reaction chamber and / or the carrier is a flat structure.

[0019] Optionally, the flat structure means that a dimension of the reaction chamber or the carrier perpendicular to its thickness direction is larger than a dimension of the reaction chamber or the carrier in its thickness direction.

[0020] Optionally, the ratio of the dimension of the reaction chamber or the carrier perpendicular to its thickness direction to its thickness direction is greater than 5:1.

[0021] Optionally, the ratio of the dimensions is 50:1 to 100:1.

[0022] The ring-shaped portion means that the side wall extends continuously until both ends are closed.

[0023] Optionally, the electromagnetic heating mechanism is used to heat the carrier, and the cooling mechanism is used to cool the carrier.

[0024] Another object of the present invention is to provide a PCR detection system to at least solve one of the above technical problems.

[0025] To achieve this purpose, the second aspect of the utility model adopts the following technical solutions:

[0026] A PCR detection system comprises an electromagnetic heating mechanism and a cooling mechanism, wherein the electromagnetic heating mechanism is used to heat the above-mentioned carrier, and the cooling mechanism is used to cool the above-mentioned carrier.

[0027] Optionally, the PCR detection system further comprises a temperature detection mechanism, and the temperature detection mechanism is used to detect the temperature of the reaction solution in the carrier.

[0028] Optionally, the temperature detection mechanism is a first temperature detection mechanism, and the first temperature detection mechanism detects the temperature of the reaction liquid by detecting the fluorescence intensity at a temperature sensing portion of the carrier.

[0029] Optionally, the first temperature detection mechanism includes:

[0030] a first fluorescent light receiving and emitting component, used for emitting excitation light of a preset intensity to the temperature sensing portion and receiving and detecting the intensity of the excitation light at the temperature sensing portion;

[0031] The first controller is electrically connected to the first fluorescent light receiving and emitting component, and is used for converting the light intensity signal detected by the first fluorescent light receiving and emitting component into a temperature signal.

[0032] Optionally, the first fluorescent light receiving assembly includes:

[0033] A first light source, used to emit the excitation light of a preset intensity;

[0034] a first incident channel connected to the first light source and used to transmit the excitation light of the first light source to the temperature sensing portion;

[0035] A first receiving channel and a first detector, wherein the first receiving channel is used to transmit the excitation light at the temperature sensing portion to the first detector, and the first detector can detect the intensity of the excitation light transmitted by the first receiving channel.

[0036] Optionally, the temperature detection mechanism is a second temperature detection mechanism, and the second temperature detection mechanism is used to detect the temperature of the reaction liquid in the carrier.

[0037] Optionally, the second temperature detection mechanism includes:

[0038] A second fluorescent temperature-sensitive portion, comprising a fluorescent temperature-sensitive material, wherein the second fluorescent temperature-sensitive portion can be located in a reaction liquid in a reaction chamber of the carrier;

[0039] a second fluorescent light receiving and emitting component, the second fluorescent light receiving and emitting component being used to emit excitation light of a preset intensity to the second fluorescent temperature-sensitive portion and to receive and detect the intensity of the excitation light at the second fluorescent temperature-sensitive portion;

[0040] The second controller is electrically connected to the second fluorescent light receiving and emitting component and is used for converting the light intensity signal detected by the second fluorescent light receiving and emitting component into a temperature signal.

[0041] Optionally, the second fluorescent light receiving assembly includes:

[0042] A second incident channel, used for transmitting the excitation light to the second fluorescent temperature-sensitive portion;

[0043] a second receiving channel, the second receiving channel being used to transmit the excitation light at the second fluorescent temperature-sensitive portion;

[0044] The second output end of the second incident channel, the second incident end of the second receiving channel and the second fluorescent temperature-sensitive portion can all be located in the reaction liquid of the reaction chamber.

[0045] Optionally, the second temperature detection mechanism further includes a light-isolating covering portion, and at least the second exit end of the second incident channel, at least the second incident end of the second receiving channel and the second fluorescent temperature-sensitive portion are all arranged in the light-isolating covering portion.

[0046] Optionally, the second fluorescent temperature-sensitive portion includes a fluorescent temperature-sensitive material coated on the second output end of the second incident channel; and / or the second fluorescent temperature-sensitive portion is connected to the second output end of the second incident channel and extends from the second incident channel.

[0047] Optionally, the cooling mechanism comprises a container in which a cooling liquid continuously flows, and the carrier can be inserted into the cooling liquid.

[0048] Optionally, the electromagnetic heating mechanism comprises a coil for passing electric current.

[0049] Optionally, the coil is disposed on the upper side or the lower side of the container, or the coil is sleeved on the outer side of the container, or the coil is disposed inside the container, and / or

[0050] The PCR detection system also includes a fluorescence detection device, which is used to detect the content of the substance to be detected in the reaction solution.

[0051] Optionally, the fluorescence detection device is arranged outside the container.

[0052] Optionally, the fluorescence detection device is located on one axial side or one radial side of the coil.

[0053] As can be seen from the above, the technical solution provided by the utility model is that the cavity wall can be heated by the electromagnetic field. Therefore, the carrier can be heated by a non-contact electromagnetic field. Since the electromagnetic heating mechanism that generates the electromagnetic field does not contact the carrier, even if the carrier generates condensed water during the heating and cooling process, the circuit of the electromagnetic heating mechanism will not be short-circuited, thereby improving the reliability of the equipment. The electromagnetic heating mechanism that generates the electromagnetic field can heat the reaction liquid in the carrier without contact with the carrier. Therefore, when heating the carrier, the time required for heat conduction between the carrier and the electromagnetic heating mechanism is saved, the heating efficiency is improved, and the PCR reaction process and PCR reaction efficiency are accelerated. It solves the problem of long detection time and slow heating and cooling speed caused by the large thermal capacity of the traditional heater and the large thermal resistance between the heater and the carrier.

[0054] The carrier is directly formed by a heat conducting sheet or a heat conducting sheet and a transparent material, which reduces the heat capacity of the carrier, increases the temperature of the reaction liquid quickly, reduces the carrier cost, and simplifies the process.

[0055] The carrier may further include a first fluorescent temperature-sensitive portion, and the first temperature detection mechanism detects the temperature of the reaction liquid by detecting the fluorescence intensity at the first fluorescent temperature-sensitive portion of the carrier. Since the thickness of the reaction chamber is very small, the temperature of the reaction liquid can be quickly and evenly distributed at various locations. At the same time, the heat transfer between the chamber wall and the reaction liquid is very fast, so the temperature of the chamber wall can be regarded as consistent with the temperature of the reaction liquid. The first fluorescent temperature-sensitive portion is arranged on the outside of the carrier and on the chamber wall, so the heat of the chamber wall can be quickly transferred to the second fluorescent temperature-sensitive portion, so that the second fluorescent temperature-sensitive portion can reflect the temperature of the reaction liquid in real time and accurately.

[0056] The cavity wall has a certain light-isolating effect. When the first fluorescent temperature-sensitive part is directly arranged on the outside of the cavity wall, the cavity wall can prevent the fluorescence used for temperature detection from entering the reaction cavity, thereby avoiding the fluorescence used for temperature detection from interfering with the detection results of the fluorescence detection device and ensuring the accuracy of PCR detection.

[0057] A light-isolating portion is also provided between the first fluorescent temperature-sensitive portion and the cavity wall. When light leaks from the cavity wall, the light-isolating portion can completely prevent the fluorescence used for temperature detection from entering the reaction cavity, completely avoiding interference of the fluorescence used for temperature detection on the detection result of the fluorescence detection device, thereby ensuring the accuracy of PCR detection.

[0058] The reaction chamber and / or carrier has a flat structure, which makes the reaction liquid in the reaction chamber very thin, and the center of the reaction liquid is very close to the surface of the liquid. When the carrier is heated / cooled, the temperature of the reaction liquid can reach a consistent level in a very short time, thereby increasing the thermal equilibrium speed of the carrier, greatly improving the reaction liquid heating and cooling speed and detection efficiency.

[0059] The PCR detection system includes an electromagnetic heating mechanism and a cooling mechanism, wherein the electromagnetic heating mechanism is used to heat the carrier, and the cooling mechanism is used to cool the carrier. The electromagnetic heating mechanism can generate an alternating magnetic field, and through the method of magnetic field eddy current heating, eddy currents are induced in the heat conducting sheet, so that the carrier is heated, and then the reaction liquid in the carrier is quickly heated. The electromagnetic heating mechanism heats the carrier through a non-contact electromagnetic field, and the electromagnetic heating mechanism does not contact the carrier. Therefore, even if condensed water is generated by the carrier during the heating and cooling process, the circuit of the electromagnetic heating mechanism will not be short-circuited, thereby improving the reliability of the equipment. Also, since the reaction liquid in the carrier can be heated without contact between the electromagnetic heating mechanism and the carrier, the non-contact electromagnetic heating mechanism heats the carrier, saving the time required for heat conduction between the carrier and the traditional heater, as well as the time required for the heater itself to heat up. The carrier and the reaction liquid heat up quickly, improving the heating efficiency, and accelerating the PCR reaction process and PCR reaction efficiency. The problem of long detection time and slow heating and cooling caused by large heat capacity of the heater and large thermal resistance between the heater and the carrier is solved.

[0060] The cooling mechanism is used to cool the carrier, thereby reducing the temperature of the reaction solution in the carrier. The electromagnetic heating mechanism and the cooling mechanism keep the reaction solution at the denaturation temperature, extension temperature and annealing temperature during the PCR reaction, respectively, and maintain them for a period of time.

[0061] When the reaction liquid needs to be cooled, the power of the electromagnetic heating mechanism can be reduced, such as increasing the duty cycle or reducing the current, and / or increasing the flow rate of the coolant. When the power of the electromagnetic heating mechanism changes, the eddy current in the carrier changes instantaneously, and the heat generated by the carrier itself also changes accordingly. Therefore, the PCR detection system can more easily and quickly adjust the temperature of the carrier and the reaction liquid. The cooling mechanism includes a container, in which a coolant continuously flows, and the carrier can be inserted into the coolant, that is, the coolant is in direct contact with the carrier. The coolant continues to flow during the PCR reaction, so the cooling mechanism is in a state of cooling the carrier at any time, so that when the carrier needs to be cooled, the carrier can be cooled quickly.

[0062] The emission wavelength of the first fluorescent light receiving component (or the second fluorescent light receiving component (ie, the second light source)) is λ 0 The monochromatic excitation light, after the excitation light emitted by the first fluorescent light receiving and emitting component (or the second fluorescent light receiving and emitting component) irradiates the first fluorescent temperature sensitive part (or the second fluorescent temperature sensitive part), has a wavelength of λ 0 The fluorescent molecules will generate light of another wavelength as the temperature of the first fluorescent temperature-sensitive portion (or the second fluorescent temperature-sensitive portion) changes, resulting in a wavelength of λ 0The intensity of the excitation light is weakened, and the first receiving channel (or the second receiving channel) collects the intensity I of the excitation light after the intensity is weakened to obtain the temperature. The PCR detection system is not affected by the monochromaticity (i.e., the wavelength distribution of the monochromatic light) of the excitation light emitted by the first fluorescent light receiving component (or the second fluorescent light receiving component (i.e., the second light source)), and can improve the detection accuracy and detection sensitivity, and ensure the accuracy of temperature control.

[0063] The second output end of the second incident channel, the second incident end of the second receiving channel and the second fluorescent temperature-sensitive portion can all be located in the reaction liquid of the reaction chamber, wherein the second fluorescent temperature-sensitive portion is located in the reaction liquid of the reaction chamber, so that the temperature of the second fluorescent temperature-sensitive portion can be kept consistent with that of the reaction liquid, thereby improving the temperature detection accuracy, and further improving the PCR detection accuracy; the second output end of the second incident channel and the second incident end of the second receiving channel are located in the reaction liquid of the reaction chamber, which can ensure that the distance between them and the second fluorescent temperature-sensitive portion is very small, thereby improving the accuracy of fluorescence intensity detection, and further ensuring the temperature detection accuracy.

[0064] The second temperature detection mechanism also includes a light-isolating covering portion, and at least the second output end of the second incident channel, at least the second incident end of the second receiving channel, and the second fluorescent temperature-sensitive portion are all arranged in the light-isolating covering portion. In this embodiment, the light used to detect the temperature is evenly distributed in the light-isolating covering portion and will not leak outside the light-isolating covering portion, which can prevent the light of the temperature detection mechanism from interfering with the detection result of the fluorescent detection device, thereby ensuring the accuracy of PCR detection. BRIEF DESCRIPTION OF THE DRAWINGS

[0065] Figure 1 It is a schematic diagram of the structure of the carrier provided by the embodiment of the utility model;

[0066] Figure 2a It is a structural schematic diagram of a PCR detection system provided by an embodiment of the utility model;

[0067] Figure 2b is a temperature rise curve of the reaction solution when the reaction solution in the carrier is heated using the PCR detection system provided in this embodiment;

[0068] Figure 3 It is a structural schematic diagram of another PCR detection system provided by an embodiment of the utility model;

[0069] Figure 4 It is a schematic diagram of the structure of the connection between the second emission end and the second fluorescent temperature-sensitive part provided by an embodiment of the utility model;

[0070] Figure 5 is a corresponding curve between the collected excitation light intensity (I) and the temperature (T) provided in an embodiment of the utility model;

[0071] Figure 6aIt is a structural schematic diagram of another carrier provided by an embodiment of the utility model;

[0072] Figure 6b It is a schematic diagram of the structure of another carrier provided by an embodiment of the utility model;

[0073] Figure 7 It is a structural schematic diagram of another PCR detection system provided by an embodiment of the utility model;

[0074] Figure 8 It is a structural schematic diagram of another PCR detection system provided by an embodiment of the utility model.

[0075] In the figure:

[0076] 1. Carrier; 11. Cavity wall; 111. First wall; 112. Second wall; 113. Side wall; 114. Transparent material; 115. Heat conducting sheet; 12. Reaction cavity; 13. First fluorescent temperature sensitive part; 14. Light-isolating part;

[0077] 2. Coil;

[0078] 3. First temperature detection mechanism; 31. First incident channel; 32. First receiving channel; 33. First fluorescence receiving and transmitting assembly; 34. First detector;

[0079] 4. Second temperature detection mechanism; 41. Second fluorescent temperature-sensitive part; 42. Second incident channel; 421. Second emission end; 43. Second receiving channel; 44. Light-isolating covering part; 45. Second fluorescent light-receiving and sending component; 46. Second detector;

[0080] 5. Container;

[0081] 6. Fluorescence detection device. DETAILED DESCRIPTION

[0082] The technical solution of the utility model is further described below in conjunction with the accompanying drawings and through specific implementation methods. It is understood that the specific embodiments described herein are only used to explain the utility model, rather than to limit the utility model. It is also necessary to explain that, for the convenience of description, only the parts related to the utility model are shown in the accompanying drawings, rather than all.

[0083] Some directional words are defined in the present utility model. Unless otherwise specified, the directional words used, such as "upper", "lower", "left", "right", "inside" and "outside", are used for ease of understanding and therefore do not constitute limitations on the protection scope of the present utility model.

[0084] In the present utility model, unless otherwise clearly specified and limited, a first feature being "above" or "below" a second feature may include that the first and second features are in direct contact, or may include that the first and second features are not in direct contact but are in contact through another feature between them. Moreover, a first feature being "above", "above" and "above" a second feature includes that the first feature is directly above and obliquely above the second feature, or simply indicates that the first feature is higher in level than the second feature. A first feature being "below", "below" and "below" a second feature includes that the first feature is directly below and obliquely below the second feature, or simply indicates that the first feature is lower in level than the second feature.

[0085] In the description of the present invention, unless otherwise clearly specified and limited, the terms "connected", "connected", and "fixed" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0086] Embodiment 1

[0087] This embodiment provides a vector 1 for use in PCR reaction and PCR detection, but is not limited thereto.

[0088] like Figure 1 As shown, the carrier 1 provided in this embodiment includes a cavity wall 11, and the cavity wall 11 encloses a reaction cavity 12 for accommodating a reaction liquid, and at least a portion of the cavity wall 11 can be heated by an electromagnetic field.

[0089] The cavity wall 11 provided in this embodiment can be heated by an electromagnetic field, so the carrier 1 can be heated by a non-contact electromagnetic field. Since the electromagnetic heating mechanism that generates the electromagnetic field does not contact the carrier 1, even if the carrier 1 generates condensed water during the heating and cooling process, the circuit of the electromagnetic heating mechanism will not be short-circuited, thereby improving the reliability of the device. The electromagnetic heating mechanism that generates the electromagnetic field can heat the reaction liquid in the carrier 1 without contact with the carrier 1. Therefore, when heating the carrier 1, the time required for heat conduction between the carrier 1 and the electromagnetic heating mechanism is saved, the heating efficiency is improved, and the PCR reaction process and PCR reaction efficiency are accelerated. The problem of long detection time and slow heating and cooling speed caused by the large thermal capacity of the traditional heater and the large thermal resistance between the heater and the carrier 1 is solved.

[0090] Optionally, at least part of the cavity wall 11 of the carrier 1 is formed by a heat conductive sheet 115, which can be heated by an electromagnetic field. The heat conductive sheet 115 has a small thickness and has a heat conductive function. Therefore, when the reaction liquid needs to be cooled, the heat of the reaction liquid can also be quickly transferred to the outside of the heat conductive sheet 115. The heat conductive sheet 115 can also be called a heat conductive film. Optionally, the thickness of the heat conductive sheet 115 can be 10-100 μm, which can improve the thermal conductivity efficiency of the carrier 1, improve the detection efficiency, and shorten the detection time while ensuring the strength of the carrier 1.

[0091] Optionally, the heat conducting sheet 115 is a metal sheet, which has a high thermal conductivity and can generate eddy currents in an alternating electromagnetic field, thereby being heated in an alternating magnetic field. Optionally, the metal sheet is made of nickel or a nickel alloy.

[0092] Optionally, the chamber wall 11 includes a first wall 111 and a second wall 112 that are arranged opposite to each other, and a side wall 113 that is arranged between the first wall 111 and the second wall 112. The side wall 113, the first wall 111 and the second wall 112 can enclose the reaction chamber 12. It can be understood that the side wall 113 is annular, and the annular means that the side wall 113 extends continuously until both ends are closed. The annular can be a polygonal ring such as a circular ring, an elliptical ring, a square ring, etc., to close the space between the first wall 111 and the second wall 112. The chamber wall 11 has a simple structure and is easy to process and form.

[0093] The reaction chamber 12 and / or the carrier 1 are flat structures, which makes the thickness of the reaction liquid in the reaction chamber 12 very thin, such as the thickness of the reaction liquid can be 0.3-1 mm, and the center of the reaction liquid is very close to the surface of the liquid. When the carrier 1 is heated / cooled, the temperature of the reaction liquid can reach a uniform level in a very short time, thereby improving the thermal equilibrium speed of the carrier 1, and greatly improving the heating and cooling speeds of the reaction liquid and the detection efficiency.

[0094] Optionally, when the carrier 1 is a flat structure, it can adapt to the flat structure of the reaction chamber 12. Specifically, the flat structure means that the dimension of the reaction chamber 12 or the carrier 1 in the thickness direction is smaller than the dimension perpendicular to the thickness direction. As an example, the ratio of the dimension perpendicular to the thickness direction to the dimension in the thickness direction is greater than 5:1. More preferably, the dimension of the carrier 1 or the reaction chamber 12 in the thickness direction is much smaller than the dimension perpendicular to the thickness direction, such as the dimension ratio is 50:1 to 100:1. As an example, the dimension ratio is 90:1. As an example, the reaction chamber 12 is a cuboid, and the ratio of the length and thickness of the cuboid can be greater than 5:1, such as 90:1. For example, the dimension of the reaction chamber 12 in the thickness direction can be 0.3-1.0 mm. In the present embodiment, the thickness of the reaction chamber 12 is 0.3-0.6 mm, and the width and length of the reaction chamber 12 are about 10 mm and 20 mm, respectively. As an example, the reaction chamber 12 may also be a cylindrical structure with a diameter to thickness ratio greater than 5:1, such as a thickness of 0.3-1.0 mm and a diameter of 5-20 mm. Of course, the cross section of the reaction chamber 12 may be polygonal, circular or elliptical.

[0095] Optionally, at least part of the cavity wall 11 is made of a transparent material 114. For example, part of the side wall 113 (eg Figure 1 as shown) or part of the first wall 111 or part of the second wall 112 (as shown Figure 6a and Figure 6b As shown in the figure, the cavity wall 11 is made of a transparent material 114. Optionally, the cavity wall 11 except the transparent material 114 is made of a heat conductive sheet 115. The excitation light reacts with the fluorescent probe / dye in the reaction solution to generate fluorescence, which can pass through the transparent material 114, so that the fluorescence is detected by the fluorescence detection device 6 to detect the content of the analyte in the reaction solution.

[0096] Optionally, the transparent material 114 may be polydimethylsiloxane, polypropylene, organic glass or polycarbonate. PMMA, PDMS, PP and PC are optically transparent materials with good biocompatibility, and thus can meet the requirements of fluorescence detection and have no effect on the reaction sample.

[0097] The carrier 1 provided in this embodiment is directly formed by a heat conducting sheet or a heat conducting sheet and a transparent material 114, which reduces the heat capacity of the carrier 1, the reaction liquid heats up quickly, the carrier 1 has a low cost, and the process is simple. For example, a flat carrier 1 can be prepared in batches using a metal sheet (metal film) material, which has a low cost and a simple process. The use of an ultra-thin carrier 1 (i.e., the dimension of the carrier 1 in the thickness direction is much smaller than the dimension in other directions) reduces the total heat capacity of the carrier 1, increases the thermal equilibrium speed of the reaction liquid and the carrier 1, and thus increases the heating and cooling speed.

[0098] like Figure 2aAs shown, this embodiment also provides a PCR detection system, which includes an electromagnetic heating mechanism and a cooling mechanism, wherein the electromagnetic heating mechanism is used to heat the carrier 1, and the cooling mechanism is used to cool the carrier 1. The electromagnetic heating mechanism can generate an alternating magnetic field, and by means of magnetic field eddy current heating, eddy currents are induced in the heat conductive sheet 115, so that the carrier 1 is heated, and then the reaction liquid in the carrier 1 is quickly heated. The electromagnetic heating mechanism heats the carrier 1 through a non-contact electromagnetic field, and the electromagnetic heating mechanism does not contact the carrier 1. Therefore, even if the carrier 1 generates condensed water during the heating and cooling process, the circuit of the electromagnetic heating mechanism will not be short-circuited, thereby improving the reliability of the device. Also, since the electromagnetic heating mechanism and the carrier 1 can heat the reaction liquid in the carrier 1 without contact, the non-contact electromagnetic heating mechanism heats the carrier 1, saving the time required for heat conduction between the carrier 1 and the traditional heater, as well as the time required for the heater itself to heat up. The carrier 1 and the reaction liquid heat up quickly, improving the heating efficiency, and accelerating the PCR reaction process and PCR reaction efficiency. The problem of long detection time and slow heating and cooling caused by large heat capacity of the heater and large thermal resistance between the heater and the carrier 1 is solved. Figure 2b As shown, after experimental verification, when the PCR detection system provided in this embodiment is used to heat the reaction solution in the carrier 1, the heating rate of the reaction solution can reach 100°C / S.

[0099] The cooling mechanism is used to cool the carrier 1, thereby reducing the temperature of the reaction solution in the carrier 1. The electromagnetic heating mechanism and the cooling mechanism keep the reaction solution at the denaturation temperature, extension temperature and annealing temperature during the PCR reaction, respectively, and maintain them for a period of time.

[0100] Exemplarily, when the reaction liquid needs to be cooled, the power of the electromagnetic heating mechanism can be reduced, such as by increasing the duty cycle or reducing the current, and / or increasing the flow rate of the coolant. When the power of the electromagnetic heating mechanism changes, the eddy current in the carrier 1 changes instantaneously, and the heat generated by the carrier 1 itself also changes. Therefore, the PCR detection system can adjust the temperature of the carrier 1 and the reaction liquid more easily and quickly.

[0101] Optionally, the cooling mechanism includes a container 5, in which a coolant continuously flows, and the carrier 1 can be inserted into the coolant, that is, the coolant is in direct contact with the carrier 1, eliminating other heat-conducting structures between the carrier and the coolant, and the carrier can be quickly cooled down. Preferably, the entire carrier 1 is inserted into the coolant, and of course, a portion of the carrier 1 can also be inserted into the coolant. By inserting the carrier 1 into the coolant, the coolant continues to flow during the PCR reaction, so that the cooling mechanism is in a state of being able to cool the carrier 1 at any time, so that when the carrier 1 needs to be cooled, the carrier 1 can be quickly cooled down.

[0102] Optionally, the electromagnetic heating mechanism includes a coil 2 for passing current, and an alternating current is passed through the coil 2 to generate a magnetic field, which then acts on the carrier 1 to generate eddy currents in the carrier 1, so that the carrier 1 is quickly heated. The electromagnetic heating mechanism has a simple structural design, is easy to produce, and has low cost.

[0103] Exemplarily, the coil 2 is disposed on the lower side of the container 5 (eg Figure 2a As shown), the coil 2 is arranged on the upper side of the container 5, or the coil 2 is sleeved on the outside of the container 5, and the coil 2 is located outside the container 5, thereby preventing the coil 2 from short-circuiting.

[0104] In other optional embodiments, the coil 2 may also be disposed in the container 5. Optionally, when the coil 2 is disposed in the container 5, a separate space may be provided in the container 5, which is used to accommodate the coil 2 and can isolate the coolant to avoid a short circuit.

[0105] The PCR detection system of this embodiment adopts the cooling form of direct contact with the coolant, that is, the coolant directly contacts the carrier 1 to cool the carrier 1, and the cooling speed is fast, which shortens the PCR reaction time. The alternating magnetic field generated by the coil 2 causes eddy currents to be induced on the heat conductive sheet 115, and then the carrier 1 generates heat by itself, realizing non-contact eddy current heating, which does not require the traditional heat conduction process between the heater and the carrier 1, and the temperature rises quickly, shortening the PCR reaction time. Non-contact heating, fast heating speed, and no condensed water that occurs during the traditional contact heating and cooling process, improves the reliability of the system.

[0106] like Figure 2a As shown, optionally, the PCR detection system further includes a fluorescence detection device 6, which is used to detect the content of the analyte in the reaction solution. The fluorescence detection device 6 can be an existing structure, and the structure of the fluorescence detection device 6 is not specifically introduced here.

[0107] Optionally, the fluorescence detection device 6 is disposed outside the container 5 to prevent the coolant from damaging the fluorescence detection device 6 .

[0108] like Figure 2a As shown, illustratively, the fluorescence detection device 6 is located on one side of the axial direction of the coil 2, so that the PCR detection system is compact. For example, the fluorescence detection device 6 is arranged on the upper side of the container 5, and at least a portion of the side wall 113 of the carrier 1 located at the upper end is made of a transparent material 114. In an optional embodiment, the side wall 113 includes a top wall and a bottom wall arranged opposite to each other, wherein the top wall is located at the upper end of the carrier 1, and the top is made of a transparent material 114. In other optional embodiments, the fluorescence detection device 6 can be arranged on one side of the radial direction of the coil 2, so as to reduce the interference of the magnetic field on the fluorescence detection device 6.

[0109] Optionally, the PCR detection system may further include a temperature detection mechanism, which is used to detect the temperature of the reaction solution, thereby accurately controlling the operation of the heating mechanism and / or the cooling mechanism. Optionally, the temperature detection mechanism detects the temperature by detecting the fluorescence intensity.

[0110] Embodiment 2

[0111] like Figure 3 As shown, the carrier 1 and PCR detection system of the second embodiment are basically the same as those of the above-mentioned first embodiment. The difference between the two is that the temperature detection mechanism is a second temperature detection mechanism 4, and the second temperature detection mechanism 4 is used to detect the temperature of the reaction liquid in the carrier 1 to better control the PCR reaction.

[0112] like Figure 3 and Figure 5 As shown, the second temperature detection mechanism 4 includes a second fluorescent temperature-sensitive part 41, a second fluorescent light-receiving and sending component 45, and a second controller, wherein the second fluorescent temperature-sensitive part 41 includes a fluorescent temperature-sensitive material, and the second fluorescent temperature-sensitive part 41 can be located in the reaction liquid in the reaction chamber 12 of the carrier 1. The second fluorescent light-receiving and sending component 45 is used to emit a preset intensity I to the second fluorescent temperature-sensitive part 41. 0 The excitation light and the excitation light I at the second fluorescent temperature sensitive part 41 are received and detected. 1 strength.

[0113] The second controller is electrically connected to the second fluorescent light receiving and emitting component 45, and is used to convert the light intensity signal detected by the second fluorescent light receiving and emitting component 45 into a temperature signal. The PCR detection system may also include a control mechanism, which detects the real-time temperature of the reaction solution through the second temperature detection mechanism 4 and feeds it back to the control mechanism, the control mechanism identifies the real-time temperature fed back, and adjusts the temperature of the carrier 1 and the reaction solution by controlling the power of the electromagnetic heating mechanism according to the temperature, so that they are respectively at the denaturation temperature, extension temperature and annealing temperature in the PCR reaction process, and maintain them for a period of time, thereby realizing PCR amplification, and the fluorescence detection device 6 performs fluorescence detection on the reaction solution after amplification.

[0114] Optionally, the fluorescent temperature-sensitive material can be at least one of rhodamine B, fluorescein, and cyanine, and of course the fluorescent temperature-sensitive material can also be other materials. In this embodiment, the temperature of the reaction solution is detected by the fluorescent temperature-sensitive material, which can achieve accurate temperature measurement with high sensitivity.

[0115] like Figure 3 As shown, optionally, the second fluorescent light receiving and emitting component 45 includes a second incident channel 42 and a second receiving channel 43, the second incident channel 42 is used to transmit the excitation light to the second fluorescent temperature-sensitive part 41, and the second receiving channel 43 is used to transmit the excitation light at the second fluorescent temperature-sensitive part 41. Optionally, the second incident channel 42 and the second receiving channel 43 are optical fibers.

[0116] like Figure 3 and Figure 5 As shown, the second fluorescent light receiving assembly 45 may further include a second light source, which is connected to the second incident channel 42 to emit excitation light to the second incident channel 42. The second light source preferably emits an excitation light with a wavelength of λ 0 of monochromatic light.

[0117] The working principle of the second fluorescence detection mechanism is as follows Figure 5 As shown, the second fluorescent light receiving assembly 45 (ie, the second light source) emits a wavelength of λ 0 The monochromatic excitation light, after the excitation light emitted by the second fluorescent light receiving and emitting component 45 irradiates the second fluorescent temperature sensitive part 41, has a wavelength of λ 0 The fluorescent molecules will generate light of another wavelength as the temperature of the second fluorescent temperature-sensitive portion 41 changes, resulting in a wavelength of λ 0 The intensity of the excitation light is weakened, and the second receiving channel 43 collects the intensity I of the excitation light after the intensity is weakened. The corresponding relationship between the intensity I and the temperature T is as follows: Figure 5 As shown, and satisfies the formula: Where T 1 and T 2 The unit is K.

[0118] The detection method provided in this embodiment is not affected by the monochromaticity (ie, wavelength distribution of monochromatic light) of the excitation light emitted by the second fluorescent light receiving and transmitting component 45 (ie, the second light source), and can improve detection accuracy and detection sensitivity, thereby ensuring the accuracy of temperature control.

[0119] The second fluorescent temperature-sensitive portion 41 includes a fluorescent temperature-sensitive material coated on the second emission end 421 of the second incident channel 42; and / or Figure 4 As shown, optionally, the second fluorescent temperature-sensitive portion 41 is connected to the second fluorescent light-receiving and sending component 45. Specifically, the second fluorescent temperature-sensitive portion 41 is connected to the second exit end 421 of the second incident channel 42 and extends from the second incident channel 42. The second fluorescent temperature-sensitive portion 41 is arranged so that the excitation light incident on the second incident channel 42 is irradiated on the second fluorescent temperature-sensitive portion 41, so as to accurately obtain the intensity of the excitation light emitted by the second incident channel 42 and the excitation light collected by the second receiving channel 43, thereby improving the detection accuracy.

[0120] The second output end 421 of the second incident channel 42, the second incident end of the second receiving channel 43 and the second fluorescent temperature-sensitive portion 41 can all be located in the reaction liquid of the reaction chamber 12, wherein the second fluorescent temperature-sensitive portion 41 is located in the reaction liquid of the reaction chamber 12, so that the temperature of the second fluorescent temperature-sensitive portion 41 can be kept consistent with that of the reaction liquid, thereby improving the temperature detection accuracy, and further improving the PCR detection accuracy; the second output end 421 of the second incident channel 42 and the second incident end of the second receiving channel 43 are located in the reaction liquid of the reaction chamber 12, which can ensure that the distance between them and the second fluorescent temperature-sensitive portion 41 is very small, thereby improving the accuracy of fluorescence intensity detection, and further ensuring the temperature detection accuracy.

[0121] Furthermore, the first incident channel 31 and the first receiving channel 32 may be arranged adjacent to each other to further improve the detection accuracy.

[0122] Preferably, the second temperature detection mechanism 4 further includes a light-isolating covering portion 44, and at least the second exit end 421 of the second incident channel 42, at least the second incident end of the second receiving channel 43, and the second fluorescent temperature-sensitive portion 41 are all arranged in the light-isolating covering portion 44. In this embodiment, the light used for detecting the temperature is evenly distributed in the light-isolating covering portion 44 and will not leak outside the light-isolating covering portion 44, which can prevent the light of the temperature detection mechanism from interfering with the detection result of the fluorescent detection device 6, thereby ensuring the accuracy of PCR detection.

[0123] Optionally, the light-isolating covering portion 44 may be a light-proof flexible bag, for example, the light-isolating covering portion 44 may be made of polypropylene (PP) or polycarbonate (PC). The second fluorescent temperature-sensitive portion 41 may also be a fluorescent liquid, which is contained in the light-isolating covering portion 44, and the second output end 421 of the second incident channel 42 and the second incident end of the second receiving channel 43 may extend into the fluorescent liquid, or be located above the fluorescent liquid.

[0124] Optionally, a socket is provided on the side wall 113 of the carrier 1, and the portion of the second temperature detection mechanism 4 located in the reaction chamber 12 can be inserted into the reaction chamber 12 through the socket. Specifically, the socket can be interference-fitted with the portion of the second temperature detection mechanism 4 inserted into the reaction chamber 12, thereby preventing water vapor from escaping during the PCR reaction. Exemplarily, the portion of the second temperature detection mechanism 4 located in the reaction chamber 12 includes the second output end 421 of the second incident channel 42, the second incident end of the second receiving channel 43, the second fluorescent temperature-sensitive portion 41, and the light-isolating covering portion 44.

[0125] Optionally, the second fluorescence receiving and transmitting assembly 45 may further include a first detector 46, which is connected to the second receiving channel 43 and is used to detect the intensity of the excitation light transmitted by the second receiving channel 43. The fluorescence detection device 6 includes a fluorescence detection detector, which is used to detect the intensity of the fluorescence. In this embodiment, the specific positions of the fluorescence detection detector and the first detector 46 are not limited, as long as the first temperature detection mechanism and the fluorescence detection device 6 can detect the corresponding fluorescence intensity.

[0126] The detector may include a silicon photomultiplier tube, a photon detector or a photomultiplier tube. The detector using the silicon photomultiplier tube, the photon detector or the photomultiplier tube has high sensitivity and can achieve ultrafast, high-sensitivity detection of fluorescence signals at the millisecond level during fluorescence detection.

[0127] Embodiment 3

[0128] like Figure 6a-Figure 7 As shown, the carrier 1 and PCR detection system of the third embodiment are basically the same as the carrier 1 and PCR detection system of the first embodiment, and the difference between the two is that the carrier 1 is also provided with a structure for detecting temperature, and the temperature detection mechanism is a first temperature detection mechanism 3, and the first temperature detection mechanism 3 is used to detect the temperature of the reaction liquid in the carrier 1 to better control the PCR reaction. Of course, the PCR detection system can also include the first temperature detection mechanism 3 and the second temperature detection mechanism 4 in the second embodiment at the same time.

[0129] The carrier 1 may further include a temperature sensing portion, which is used to reflect the temperature of the reaction liquid, thereby facilitating the first temperature detection mechanism 3 to detect the temperature of the reaction liquid.

[0130] like Figure 6a As shown, the temperature sensing part includes a first fluorescent temperature-sensitive part 13, and the first temperature detection mechanism 3 detects the temperature of the reaction liquid by detecting the fluorescence intensity at the first fluorescent temperature-sensitive part 13 of the carrier 1. Optionally, the first fluorescent temperature-sensitive part 13 is arranged on the outside of the cavity wall 11, and the first fluorescent temperature-sensitive part 13 includes a fluorescent temperature-sensitive material, and the first fluorescent temperature-sensitive part 13 can reflect the temperature of the reaction liquid. The fluorescent temperature-sensitive material of the first fluorescent temperature-sensitive part 13 can be at least one of rhodamine B, fluorescein, and cyanine. Since the thickness of the reaction chamber 12 is very small, the temperature of each part of the reaction liquid can be quickly distributed evenly. At the same time, the heat transfer between the cavity wall 11 and the reaction liquid is very fast, so the temperature of the cavity wall 11 can be regarded as consistent with the temperature of the reaction liquid. The first fluorescent temperature-sensitive part 13 is arranged on the outside of the carrier 1 and on the cavity wall 11. Therefore, the heat of the cavity wall 11 can be quickly conducted to the second fluorescent temperature-sensitive part 41, so that the second fluorescent temperature-sensitive part 41 can reflect the temperature of the reaction liquid in real time and accurately.

[0131] In addition, the first fluorescent temperature-sensitive portion 13 can be arranged on the heat-conducting sheet 115, so that the cavity wall 11 has a certain light-isolating effect. When the first fluorescent temperature-sensitive portion 13 is directly arranged on the outside of the cavity wall 11, the cavity wall 11 can prevent the fluorescence used for temperature detection from entering the reaction chamber 12, thereby preventing the fluorescence used for temperature detection from interfering with the detection result of the fluorescence detection device 6, and ensuring the accuracy of PCR detection. Of course, in other optional embodiments, the first fluorescent temperature-sensitive portion 13 can also be arranged on the transparent material 114.

[0132] like Figure 6b As shown, further, a light-isolating portion 14 is further provided between the first fluorescent temperature-sensitive portion 13 and the cavity wall 11. In this way, whether the first fluorescent temperature-sensitive portion 13 is provided on the heat-conducting sheet 115 or on a transparent material, the light-isolating portion 14 can completely prevent the fluorescence used for temperature detection from entering the reaction chamber 12, completely avoiding the interference of the fluorescence used for temperature detection on the detection result of the fluorescence detection device 6, and ensuring the accuracy of PCR detection. The light-isolating portion 14 can be selected to be black, or the light-isolating portion 14 can also be opaque, and the light-isolating portion 14 can preferably be made of metal material.

[0133] Optionally, the light-isolating portion 14 can also be a black coating coated on the outside of the cavity wall 11. The specific material of the light-isolating portion 14 is not limited here, as long as the light-isolating portion 14 is insoluble in the coolant and can be coated on the outside of the cavity wall 11 and has a heat-conducting function. The first fluorescent temperature-sensitive portion 13 is a fluorescent temperature-sensitive material layer coated on the light-isolating portion 14.

[0134] like Figure 6b-Figure 7 As shown, specifically, the first fluorescent temperature-sensitive portion 13 is arranged on the outside of the first wall 111, and at least part of the second wall 112 is made of transparent material 114. The excitation light emitted by the fluorescent detection device 6 passes through the transparent material 114 located on the second wall 112 for detection. The first temperature detection mechanism 3 detects the temperature through the first fluorescent temperature-sensitive portion 13 arranged on the first wall 111. The first wall 111 and the second wall 112 are arranged relative to each other, so that the mutual influence between the temperature detection and the content detection of the object to be detected can be further avoided. Of course, in other optional embodiments, at least part of the side wall 113 can also be made of transparent material 114, and the fluorescent detection device 6 detects through the transparent material 114 of the side wall 113.

[0135] like Figure 7 As shown, optionally, the first temperature detection mechanism 3 includes a first fluorescent receiving and transmitting component 33 and a first controller, wherein the first fluorescent receiving and transmitting component 33 is used to emit excitation light of preset intensity to the first fluorescent temperature-sensitive part 13 and receive and detect the intensity of the excitation light at the first fluorescent temperature-sensitive part 13, and the first controller is electrically connected to the first fluorescent receiving and transmitting component 33, and is used to convert the light intensity signal detected by the first fluorescent receiving and transmitting component 33 into a temperature signal.

[0136] It can be understood that the temperature measurement principle of the first temperature detection mechanism 3 and the second temperature detection mechanism 4 is the same, and the difference between the two is that the first temperature detection mechanism 3 does not need to extend into the reaction liquid to detect the temperature, and the first temperature detection mechanism 3 can perform temperature detection on one side of the first fluorescent temperature-sensitive portion 13. In this embodiment, the first fluorescent temperature-sensitive portion 13 prepared can achieve accurate temperature measurement with high sensitivity.

[0137] Specifically, the first fluorescent light-receiving and transmitting assembly 33 may include a first light source, a first incident channel 31, a first receiving channel 32 and a first detector 34. The first light source is used to emit excitation light of a preset intensity, the first incident channel 31 is connected to the first light source, and is used to transmit the excitation light of the first light source to the first fluorescent temperature-sensitive portion 13, the first receiving channel 32 is used to transmit the excitation light at the first fluorescent temperature-sensitive portion 13 to the first detector 34, and the first detector 34 can detect the intensity of the excitation light transmitted by the first receiving channel 32.

[0138] like Figure 7 As shown, optionally, the first incident channel 31 and the first receiving channel 32 can be optical fibers. Optionally, the first emission end of the first incident channel 31 and the first incident end of the first receiving channel 32 are both located on one side of the first fluorescent temperature-sensitive portion 13 to ensure the accuracy of the detection result. Furthermore, the first incident channel 31 and the first receiving channel 32 can be arranged adjacent to each other to further improve the detection accuracy. Figure 8 As shown, the first incident channel 31 and the first receiving channel 32 may not be specific physical objects, but are propagation channels of light in space.

[0139] Embodiment 4

[0140] This embodiment provides a PCR amplification method, which can use the vector 1 provided in the first embodiment and the PCR detection system provided in the second embodiment to perform the PCR amplification method of the fourth embodiment, or use the vector 1 provided in the first embodiment and the PCR detection system provided in the third embodiment to perform the PCR amplification method of the fourth embodiment.

[0141] PCR amplification methods include:

[0142] The coolant continuously flows in the cooling mechanism to continuously cool the carrier 1;

[0143] The electromagnetic heating mechanism generates a magnetic field and heats the carrier 1 through the magnetic field.

[0144] The electromagnetic heating mechanism generates a magnetic field, causing the carrier 1 to heat up and quickly increase the temperature, thereby increasing the temperature of the reaction liquid in the carrier 1. The cooling mechanism is used to cool the carrier 1, thereby reducing the temperature of the reaction liquid in the carrier 1. The electromagnetic heating mechanism and the cooling mechanism keep the reaction liquid at the denaturation temperature, extension temperature, and renaturation temperature during the PCR reaction, respectively, and maintain them for a period of time. The coolant in the cooling mechanism flows continuously, and can be prepared for cooling at any time to achieve rapid cooling.

[0145] The electromagnetic heating mechanism heats the carrier 1 through a non-contact electromagnetic field. The electromagnetic heating mechanism does not contact the carrier 1. Therefore, even if condensed water is generated in the carrier 1 during the heating and cooling process, the circuit of the electromagnetic heating mechanism will not be short-circuited, thereby improving the reliability of the device. Also, since the electromagnetic heating mechanism and the carrier 1 can heat the reaction liquid in the carrier 1 without contact, when heating the carrier 1, the time required for heat conduction between the carrier 1 and the electromagnetic heating mechanism is saved, thereby achieving rapid heating and accelerating the PCR reaction process and PCR reaction efficiency. The problem of long detection time and slow heating and cooling speed caused by large heat capacity of the heater and large thermal resistance between the heater and the carrier 1 is solved.

[0146] Optionally, the coolant continuously flows in the cooling mechanism to continuously cool the carrier 1, including:

[0147] The carrier 1 is in direct contact with the cooling liquid.

[0148] The carrier 1 is in direct contact with the coolant, eliminating other heat-conducting structures between the carrier 1 and the coolant, and can achieve rapid cooling of the carrier 1.

[0149] Optionally, the temperature of the carrier 1 is adjusted by adjusting the power of the electromagnetic heating mechanism, and / or by adjusting the temperature and / or flow rate of the coolant in the cooling mechanism. Exemplarily, when the reaction liquid needs to be cooled, the power of the electromagnetic heating mechanism can be reduced, such as increasing the duty cycle or reducing the current, and / or increasing the flow rate of the coolant; when the temperature needs to be increased, the power of the electromagnetic heating mechanism can be increased, such as reducing the duty cycle or increasing the current, and / or reducing the flow rate of the coolant.

[0150] The PCR detection method provided in this embodiment can achieve rapid heating and cooling. For example, compared with the traditional contact heater, the cooling mechanism needs to cool the heater, and then cool the carrier 1 through the heater. The intermediate heat conduction takes a long time. The cooling mechanism in this embodiment does not need to cool the electromagnetic heating mechanism, but directly cools the carrier 1, so the cooling speed can be accelerated. For another example, when the contact heater heats the carrier 1, the heat conduction between the carrier 1 and the heater takes a certain amount of time, and the heater itself also takes time to heat up. In this embodiment, the electromagnetic heating mechanism directly generates eddy currents in the carrier 1, and the eddy currents cause the carrier 1 to heat up, and then heat the reaction liquid in the carrier 1, thereby greatly reducing the time required for heat conduction, thereby improving the heating efficiency of the reaction liquid. For another example, the coolant flows continuously. During the PCR reaction process, there is no need to pass coolants of different temperatures to achieve temperature rise and fall, which can greatly reduce the reaction time of the cooling mechanism and is conducive to achieving rapid temperature rise and fall.

[0151] PCR amplification methods also include:

[0152] The temperature of the reaction liquid is detected by the fluorescent temperature-sensitive material and the intensity of the excitation light. This temperature detection method has high sensitivity and can achieve accurate temperature measurement.

[0153] Detecting the temperature of the reaction solution by using fluorescent temperature-sensitive materials and the intensity of the excitation light includes:

[0154] Continuously emitting excitation light to the fluorescent temperature-sensitive material;

[0155] Collect the excitation light from the fluorescent temperature-sensitive material,

[0156] like Figure 5 As shown, the intensity of the collected excitation light is obtained, and the temperature of the reaction liquid is obtained through the intensity of the collected excitation light.

[0157] By continuously emitting excitation light to the fluorescent temperature-sensitive material, the temperature of the carrier 1 can be detected in real time.

[0158] The emission wavelength of the temperature detection mechanism is λ 0 The monochromatic excitation light, after irradiating the fluorescent temperature-sensitive material, has a wavelength of λ 0 The fluorescent molecules will produce light of another wavelength as the temperature of the fluorescent thermosensitive material changes, resulting in a wavelength of λ 0 The intensity of the excitation light is weakened, and the intensity I of the excitation light after the intensity is weakened is collected. The corresponding relationship between the intensity I and the temperature T is as follows: Figure 5 As shown, and satisfies the formula: Where T 1 and T 2 The unit is K.

[0159] The PCR detection method is not affected by the monochromaticity of the excitation light (i.e., the wavelength distribution of the monochromatic light), and can improve the detection accuracy and sensitivity and ensure the accuracy of temperature control.

[0160] Although the utility model has been described in detail above by means of general description, specific implementation methods and tests, it is obvious to those skilled in the art that some modifications or improvements can be made to the utility model. Therefore, these modifications or improvements made without departing from the spirit of the utility model are within the scope of protection claimed by the utility model.

Claims

1. A carrier, characterized in that The invention comprises a cavity wall (11), wherein the cavity wall (11) comprises a first wall (111) and a second wall (112) arranged opposite to each other, and a side wall (113) arranged between the first wall (111) and the second wall (112), wherein the side wall (113) is annular, and the cavity wall (11) encloses a reaction cavity (12) for accommodating a reaction liquid, and at least a portion of the cavity wall (11) can be heated by an electromagnetic field.

2. The carrier according to claim 1, characterized in that The carrier further includes a temperature sensing portion, and the temperature sensing portion is used to reflect the temperature of the reaction liquid.

3. The carrier according to claim 2, characterized in that The temperature sensing part comprises a first fluorescent temperature-sensitive part (13) arranged on the outside of the cavity wall (11), the first fluorescent temperature-sensitive part (13) comprises a fluorescent temperature-sensitive material, and the first fluorescent temperature-sensitive part (13) can reflect the temperature of the reaction liquid.

4. The carrier according to claim 3, characterized in that A light-isolating portion (14) is also provided between the first fluorescent temperature-sensitive portion (13) and the cavity wall (11).

5. The carrier according to any one of claims 2 to 4, characterized in that The temperature sensing portion is arranged on the outside of the first wall (111), and at least a portion of the second wall (112) or at least a portion of the side wall (113) is made of a transparent material (114).

6. The carrier according to claim 1, characterized in that At least part of the cavity wall (11) is formed by a heat conductive sheet (115), and the heat conductive sheet (115) can be heated by an electromagnetic field.

7. The carrier according to claim 6, characterized in that The heat conducting sheet (115) is a metal sheet.

8. The carrier according to claim 6 or 7, characterized in that The thickness of the heat conducting sheet (115) is 10-100 μm.

9. The carrier according to claim 1, characterized in that At least a portion of the cavity wall (11) is made of a transparent material (114).

10. The carrier according to claim 9, characterized in that The transparent material (114) is polydimethylsiloxane, polypropylene, organic glass or polycarbonate.

11. The carrier according to any one of claims 1-4, 6-7, 9-10, characterized in that: The reaction chamber (12) and / or the carrier are flat structures.

12. The carrier according to claim 11, characterized in that The flat structure means that the dimension of the reaction chamber (12) or the carrier perpendicular to its thickness direction is larger than its thickness direction.

13. The carrier according to claim 12, characterized in that The ratio of the dimension of the reaction chamber (12) or the carrier perpendicular to its thickness direction to its thickness direction is greater than 5:

1.

14. The carrier according to claim 13, characterized in that The size ratio is 50:1 to 100:

1.

15. The carrier according to claim 1, characterized in that The ring-shaped shape means that the side wall (113) extends continuously until both ends are closed.

16. A PCR detection system, characterized in that: It comprises an electromagnetic heating mechanism and a cooling mechanism, wherein the electromagnetic heating mechanism is used to heat the carrier described in any one of claims 1 to 15, and the cooling mechanism is used to cool the carrier described in any one of claims 1 to 15.

17. The PCR detection system according to claim 16, characterized in that: The PCR detection system further comprises a temperature detection mechanism, and the temperature detection mechanism is used to detect the temperature of the reaction solution in the carrier.

18. The PCR detection system according to claim 17, characterized in that: The temperature detection mechanism is a first temperature detection mechanism (3), and the first temperature detection mechanism (3) detects the temperature of the reaction liquid by detecting the fluorescence intensity at the temperature sensing part of the carrier.

19. The PCR detection system according to claim 18, characterized in that: The first temperature detection mechanism (3) comprises: A first fluorescent light receiving and emitting component (33) is used to emit excitation light of a preset intensity to the temperature sensing portion and to receive and detect the intensity of the excitation light at the temperature sensing portion; The first controller is electrically connected to the first fluorescent light receiving component (33) and is used to convert the light intensity signal detected by the first fluorescent light receiving component (33) into a temperature signal.

20. The PCR detection system according to claim 19, characterized in that: The first fluorescent light receiving assembly (33) comprises: A first light source, used to emit the excitation light of a preset intensity; A first incident channel (31), connected to the first light source and used to transmit the excitation light of the first light source to the temperature sensing portion; A first receiving channel (32) and a first detector (34), wherein the first receiving channel (32) is used to transmit the excitation light at the temperature sensing portion to the first detector (34), and the first detector (34) is capable of detecting the intensity of the excitation light transmitted by the first receiving channel (32).

21. The PCR detection system according to claim 17, characterized in that: The temperature detection mechanism is a second temperature detection mechanism (4), and the second temperature detection mechanism (4) is used to detect the temperature of the reaction liquid in the carrier.

22. The PCR detection system according to claim 21, characterized in that: The second temperature detection mechanism (4) comprises: A second fluorescent temperature-sensitive portion (41), comprising a fluorescent temperature-sensitive material, wherein the second fluorescent temperature-sensitive portion (41) can be located in a reaction liquid in a reaction chamber (12) of the carrier; A second fluorescent light receiving and emitting component (45), the second fluorescent light receiving and emitting component (45) being used to emit excitation light of a preset intensity to the second fluorescent temperature-sensitive portion (41) and to receive and detect the intensity of the excitation light at the second fluorescent temperature-sensitive portion (41); The second controller is electrically connected to the second fluorescent light receiving component (45) and is used to convert the light intensity signal detected by the second fluorescent light receiving component (45) into a temperature signal.

23. The PCR detection system according to claim 22, characterized in that: The second fluorescent light receiving assembly (45) comprises: A second incident channel (42), used for transmitting the excitation light to the second fluorescent temperature-sensitive portion (41); a second receiving channel (43), the second receiving channel (43) being used to transmit the excitation light at the second fluorescent temperature-sensitive portion (41); The second output end (421) of the second incident channel (42), the second incident end of the second receiving channel (43) and the second fluorescent temperature-sensitive portion (41) can all be located in the reaction liquid of the reaction chamber (12).

24. The PCR detection system according to claim 23, characterized in that: The second temperature detection mechanism (4) further comprises a light-isolating covering portion (44), wherein at least the second output end (421) of the second incident channel (42), at least the second incident end of the second receiving channel (43) and the second fluorescent temperature-sensitive portion (41) are all arranged in the light-isolating covering portion (44).

25. The PCR detection system according to claim 23, characterized in that: The second fluorescent temperature-sensitive portion (41) comprises a fluorescent temperature-sensitive material coated on the second output end (421) of the second incident channel (42); and / or the second fluorescent temperature-sensitive portion (41) is connected to the second output end (421) of the second incident channel (42) and extends from the second incident channel (42).

26. The PCR detection system according to any one of claims 16 to 25, characterized in that: The cooling mechanism comprises a container (5), in which a cooling liquid continuously flows, and the carrier can be inserted into the cooling liquid.

27. The PCR detection system according to claim 26, characterized in that: The electromagnetic heating mechanism comprises a coil (2) for passing electric current.

28. The PCR detection system according to claim 27, characterized in that: The coil (2) is arranged on the upper side or the lower side of the container (5), or the coil (2) is sleeved on the outside of the container (5), or the coil (2) is arranged inside the container (5), and / or The PCR detection system further comprises a fluorescence detection device (6), wherein the fluorescence detection device (6) is used to detect the content of the substance to be detected in the reaction solution.

29. The PCR detection system according to claim 28, characterized in that: The fluorescence detection device (6) is arranged outside the container (5).

30. The PCR detection system according to claim 28, characterized in that: The fluorescence detection device (6) is located on one axial side or one radial side of the coil (2).

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