Full-automatic fluorescence imaging and quantitative analysis microscope

Abstract

The utility model discloses a full-automatic fluorescence imaging and quantitative analysis microscope, and relates to the technical field of microscopes. The fluorescent wheel assembly comprises a fluorescent wheel, a housing; the cover plate is fixedly arranged at the top end of the shell, and an inner cavity is formed therebetween; the rotating piece is fixedly arranged at the bottom end of the inner cavity; the rotating rod is arranged in the inner cavity and is fixedly mounted at the top end of the rotating part; the fluorescent light filters are arranged in the inner cavity and are uniformly fixed outside the rotating rod in a surrounding manner; the turntable is arranged in the inner cavity and is fixed with the top end of the rotating rod; the penetrating opening is formed between the shell and the cover plate and communicates the interior and exterior of the inner cavity; the fluorescent lamp has the advantages that the gear can be driven to rotate and meshed to drive the rotating disc to rotate by starting the forward and reverse rotation motor to rotate, the rotating rod is integrally connected with the rotating disc and the fluorescent light filter, namely the rotating rod is synchronously driven to rotate, the rotating piece provides rotating support, the position of the fluorescent light filter is replaced, and operation is easy and convenient; the fluorescent light filter is simple and convenient to disassemble and assemble; and the cleanliness in the inner cavity is good.

Description

Technical Field

[0001] The utility model relates to the technical field of microscopes, and more specifically to a full-automatic fluorescence imaging and quantitative analysis microscope. Background Art

[0002] Fluorescence microscope: Fluorescence microscope uses ultraviolet light as a light source to illuminate the object being inspected, causing it to emit fluorescence, and then observe the shape and location of the object under the microscope;

[0003] The fully automatic fluorescence imaging and quantitative analysis microscope is a highly integrated precision instrument that combines modern optics, electronics, computer science and automation technology {such as the publication (publication) number: CN217506262U, a gantry fluorescence microscope for observing awake mice} with a similar structure "wherein the turntable can be replaced with a forward and reverse motor, a motor to provide automatic adjustment";

[0004] The working principle is as follows: a camera is used in conjunction with an objective lens to shoot and record a stage on which an object to be observed is placed; the camera is connected to a display computer to display and analyze the image;

[0005] Specifically, a filter wheel with multiple sets of filter wheels installed between the camera and the objective lens is used as a collection medium to meet the imaging requirements of different fluorescent markers. The filter wheel area is equipped with a fluorescent light source (the fluorescent light source can be a type of Kohler illumination module, etc.) to provide bright light, and a backlight source is also provided under the stage to provide bright light. The position of the stage and the objective lens is generally controlled by a connector for X-axis translation and Z-axis focusing, and is used in conjunction with a controller.

[0006] The existing microscope generally uses a manual position adjustment method when adjusting the filter wheel position, which is relatively troublesome to operate. Utility Model Content

[0007] The purpose of the utility model is to solve the above technical problems and provide a fully automatic fluorescence imaging and quantitative analysis microscope.

[0008] In order to achieve the above-mentioned purpose, the utility model specifically adopts the following technical solutions:

[0009] The utility model proposes a fully automatic fluorescence imaging and quantitative analysis microscope, including a fluorescence wheel component;

[0010] The fluorescent wheel assembly comprises:

[0011] shell;

[0012] A cover plate, which is fixed to the top of the shell and forms an inner cavity therebetween;

[0013] A rotating member fixedly mounted at the bottom end of the inner cavity;

[0014] A rotating rod, which is disposed in the inner cavity and fixed to the top of the rotating member;

[0015] A plurality of groups of fluorescent filters are arranged in the inner cavity and are evenly fixed around the rotating rod;

[0016] A turntable, which is disposed in the inner cavity and fixed to the top end of the rotating rod;

[0017] A through-hole is formed between the outer shell and the cover plate and communicates the inner and outer sides of the inner cavity, and the rotating disk part moves in the through-hole;

[0018] a gear meshing with the outer periphery of the rotating disk;

[0019] The output shaft of the forward and reverse motor is fixedly connected to the gear and fixedly mounted outside the housing;

[0020] Two groups of collection ports are symmetrically arranged at the top and bottom ends of the shell and are connected to a group of fluorescent filters.

[0021] As a preferred technical solution of the utility model, the rotating rod and the rotating disk are detachably fixed by bolts.

[0022] As a preferred technical solution of the utility model, several groups of blocks are fixedly arranged on the periphery of the rotating rod corresponding to several groups of fluorescent filters, and a card slot is arranged outside the fluorescent filter. The outside of the card block and the card slot are detachably fixed and can be separated only by sliding up and down, and the bottom end of the card block is supported by the top end of the rotating part.

[0023] As a preferred technical solution of the utility model, a soft compression layer which is stably fitted with the card block is provided in the card slot.

[0024] As a preferred technical solution of the utility model, the fluorescent wheel assembly also includes a sealing patch, which is fixed in the through opening and fits with the rotating disk to seal the inner cavity.

[0025] As a preferred technical solution of the utility model, it also includes:

[0026] A bracket, the outer side of which is used for mounting the fluorescent wheel assembly;

[0027] A base, the outer side of which is provided for mounting the bracket;

[0028] An infinity tube lens, which is fixed on the top of the housing;

[0029] A scientific camera, which is fixed on the top of the infinity tube lens;

[0030] A fluorescent light source group, a telescopic tube, a first objective lens arm and a second objective lens arm are all fixedly mounted on the bracket;

[0031] The adjustment seat, the backlight source group and the controller are all fixedly mounted on the base;

[0032] The loading platform is fixed on the adjustment seat;

[0033] A first objective lens, which is fixedly mounted at the bottom end of the first objective lens arm;

[0034] A second objective lens, which is fixedly mounted on the top end of the second objective lens arm;

[0035] The stage is located between the first objective lens and the second objective lens, the bottom end of the telescopic tube is fixed to the first objective lens arm, and the collection port, the fluorescent filter, the telescopic tube, the first objective lens, the second objective lens and the backlight source group are sequentially located below the infinity tube lens.

[0036] The beneficial effects of the utility model are as follows:

[0037] By starting the forward and reverse motor 109, the gear 108 can be driven to rotate and mesh to drive the turntable 106 to rotate. The rotating rod 103 is connected to the turntable 106 and the fluorescent filter 105, that is, the rotating rod 103 is driven to rotate synchronously and the rotating member 102 provides rotation support, so that the position of the fluorescent filter 105 can be replaced, and the operation is simple. BRIEF DESCRIPTION OF THE DRAWINGS

[0038] Figure 1 It is a structural schematic diagram of the utility model;

[0039] Figure 2 yes Figure 1 Schematic diagram of the local structure;

[0040] Figure 3 It is a schematic diagram of the local structure of the fluorescent wheel assembly of the utility model.

[0041] Figure numerals: fluorescent wheel assembly-1, bracket-2, base-3, infinity tube lens-4, scientific camera-5, fluorescent light source group-6, telescopic tube-7, first objective lens arm-8, first objective lens-9, stage-10, adjustment seat-11, second objective lens arm-12, second objective lens-13, backlight source group-14, controller-15, housing-101, rotating part-102, rotating rod-103, block-104, fluorescent filter-105, turntable-106, cover plate-107, gear-108, forward and reverse motor-109, collection port-1010, through port-1011, sealing patch-1012. DETAILED DESCRIPTION Embodiment 1:

[0042] like Figure 1 As shown, this embodiment proposes: a fully automatic fluorescence imaging and quantitative analysis microscope, including a fluorescence wheel assembly 1;

[0043] The fluorescent wheel assembly 1 comprises:

[0044] Housing 101;

[0045] The cover plate 107 is fixed to the top of the housing 101 and forms an inner cavity (such as Figure 1 , 2 As shown, the cover plate 107 can be fixed to the top of the housing 101 by bolts and other components, so as to fit together, and the inner cavity is formed between the inside of the housing 101 and the bottom of the cover plate 107);

[0046] The rotating member 102 is fixedly mounted at the bottom end of the inner cavity (such as Figure 2 As shown, it is installed at the bottom end of the housing 101 and can be installed by bolts or other components) (the rotating member 102 can be a thrust bearing, etc.);

[0047] The rotating rod 103 is arranged in the inner cavity and fixed to the top of the rotating member 102 (such as Figure 2 As shown, the rotating rod 103 is fixed at the top of the rotating member 102 to provide rotation support for the rotating rod 103);

[0048] A plurality of groups of fluorescent filters 105 are arranged in the inner cavity and are evenly fixed to the outside of the rotating rod 103 (such as Figure 3 As shown, multiple groups of fluorescent filters 105 are evenly distributed in a ring shape around the rotating rod 103, which provides convenience and accuracy for subsequent adjustment and replacement of different fluorescent filters 105);

[0049] The rotating disk 106 is arranged in the inner cavity and fixed to the top of the rotating rod 103 (such as Figure 2 As shown, it is at the top of the rotating rod 103 and is assembled into one piece using bolts and other components for easy disassembly, assembly and replacement);

[0050] The through hole 1011 is formed between the housing 101 and the cover plate 107 and communicates the inside and outside of the inner cavity. The rotating disk 106 partially moves in the through hole 1011 (such as Figure 2 As shown, the through hole 1011 is located at the right end of the inner cavity, and the through hole 1011 is the first opening between the cover plate 107 and the outer shell 101);

[0051] The gear 108 meshes with the outer periphery of the rotating disk 106 (i.e., the outer periphery of the rotating disk 106 is also provided with an annular gear tooth and meshes with the gear 108);

[0052] The forward and reverse motor 109, whose output shaft is fixedly connected to the gear 108 and is fixedly mounted outside the housing 101, provides power to drive the turntable 106 to rotate, and is used to drive the rotating rod 103 and the plurality of groups of fluorescent filters 105 to rotate and transpose according to the above;

[0053] Two groups of collection ports 1010 are symmetrically arranged at the top and bottom ends of the housing 101 and connected to a group of fluorescent filters 105 (such as Figure 1 , 2 As shown, a group of fluorescent filters 105 is centrally arranged between the two groups of collection ports 1010).

[0054] like Figure 1 As shown, it also includes a bracket 2, the outer side of which is used for mounting the fluorescent wheel assembly 1 (the fluorescent wheel assembly 1 is mounted on the top of the bracket 2);

[0055] The base 3 has an outer side for mounting the bracket 2 (the bracket 2 is mounted on the top of the base 3);

[0056] The infinity tube lens 4 is fixedly mounted on the top of the housing 101 (the infinity tube lens 4 is centered on the above-mentioned collection port 1010 and covers the top of the housing 101);

[0057] A scientific camera 5 is fixedly mounted on the top of the infinity tube lens 4 (the scientific camera 5 is provided with an electrical connection line for connecting to a display computer);

[0058] The fluorescent light source group 6, the telescopic tube 7, the first objective lens arm 8 and the second objective lens arm 12 are all fixedly mounted on the bracket 2 (the fluorescent light source group 6 is mounted on the left end of the fluorescent wheel assembly 1, and is embedded in the interior of the housing 101 to provide a bright environment for the use of the fluorescent filter 105 inside the inner cavity), (the telescopic tube 7 is a corrugated hose, which is located below the housing 101 and provides a vertically telescopic connection between the first objective lens arm and the bracket 2, and the bracket 2 is provided with a second opening for the collection port 1010 to communicate with the interior of the telescopic tube 7), (the first objective lens arm 8 is located above the second objective lens arm 12);

[0059] The adjustment seat 11, the backlight source group 14 and the controller 15 are all fixedly mounted on the base 3;

[0060] The loading platform 10 is fixed on the adjustment seat 11;

[0061] A first objective lens 9, which is fixed to the bottom end of the first objective lens arm 8;

[0062] The second objective lens 13 is fixedly mounted on the top of the second objective lens arm 12 (the second objective lens arm 12 is provided with a third opening for the second objective lens 13 to connect to the backlight source group 14);

[0063] The stage 10 is between the first objective lens 9 and the second objective lens 13, the bottom end of the telescopic tube 7 is fixed to the first objective lens arm 8, the collection port 1010, the fluorescent filter 105, the telescopic tube 7, the first objective lens 9, the second objective lens 13 and the backlight source group 14 are sequentially located below the infinity tube lens 4 (the first objective lens arm 8 and the second objective lens arm 12 are translation stages for adjusting up and down, and the adjustment seats 11 are translation stages for adjusting the X, Y and Z axes);

[0064] When in use, the electrical connection line outside the scientific camera 5 is connected to the powered display computer, the controller 15 is connected to the external power supply, the sample to be observed is set on the stage 10, the controller 15 is operated to adjust the positions of the first objective lens arm 8, the second objective lens arm 12, and the adjustment seat 11 to improve the automation, the second objective lens 13 irradiates the light emitted by the backlight source group 14 onto the sample on the stage 10, the first objective lens 9 magnifies the sample on the stage 10 and transmits it to the infinity tube lens 4 through the fluorescent filter 105, and is collected by the scientific camera 5 and displayed on the display computer. The image analysis software, automatic control software, data management and storage set by the display computer itself are used to analyze and process the collected images; when the fluorescent filter 105 needs to be replaced for use, it is only necessary to start the forward and reverse motor 109 to change the position of several groups of fluorescent filters 105, which is easy to operate. Embodiment 2:

[0065] like Figure 1 As shown, the difference between it and the embodiment 1 is that: a plurality of groups of clamping blocks 104 are fixedly arranged on the periphery of the rotating rod 103 corresponding to a plurality of groups of fluorescent filters 105, a clamping slot is arranged on the outside of the fluorescent filter 105, the outside of the clamping block 104 and the clamping slot are detachably fixed and can be separated only by sliding up and down, and the bottom end of the clamping block 104 is supported by the top end of the rotating member 102 (as shown in the figure, the clamping block 104 is in a "trapezoidal" shape, and the clamping slot matched therewith is also in a "trapezoidal" shape, so that when assembling, a connection method from top to bottom is adopted, which is easy to disassemble and assemble; and the bottom end of the fluorescent filter 105 is in contact with the top end of the rotating member 102, providing support and stability);

[0066] Among them, a soft compression layer (the soft compression layer can be made of rubber or other materials) that fits stably with the card block 104 is provided in the card slot to increase the stability of the card block 104 when and after it moves in the card slot. Embodiment 3:

[0067] like Figure 1 As shown, the difference between it and Example 1 or Example 2 is that the fluorescent wheel assembly 1 also includes a sealing patch 1012, which is fixed in the through-opening 1011 and fits with the turntable 106 to seal the inner cavity (the sealing patch 1012 can be made of rubber or other materials, elastically pressed on the surface of the turntable 106 to prevent dust on the outside of the shell 101 from entering the inner cavity, thereby improving the cleanliness).

[0068] The control method of the present invention is automatic control through a controller. The control circuit of the controller can be realized by simple programming by technicians in this field. The provision of power is also common knowledge in this field. The present invention is mainly used to protect mechanical devices, so the present invention will no longer explain the control method and circuit connection in detail.

[0069] The above shows and describes the basic principles and main features of the utility model and the advantages of the utility model. For those skilled in the art, it is obvious that the utility model is not limited to the details of the above exemplary embodiments, and can be implemented in other specific forms without departing from the spirit or basic features of the utility model. Therefore, no matter from which point of view, the embodiments should be regarded as exemplary and non-restrictive. The scope of the utility model is limited by the attached claims rather than the above description, and it is intended to include all changes within the meaning and scope of the equivalent elements of the claims. Any figure mark in the claims should not be regarded as limiting the claims involved.

[0070] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. A fully automatic fluorescence imaging and quantitative analysis microscope, comprising a fluorescence wheel assembly (1); It is characterized in that The fluorescent wheel assembly (1) comprises: Shell (101); A cover plate (107) is fixedly mounted on the top of the housing (101) to form an inner cavity; A rotating member (102) fixedly mounted at the bottom end of the inner cavity; A rotating rod (103) is disposed in the inner cavity and fixed to the top end of the rotating member (102); A plurality of groups of fluorescent filters (105) are arranged in the inner cavity and are evenly fixed around the outside of the rotating rod (103); A rotating disk (106) is disposed in the inner cavity and fixed to the top end of the rotating rod (103); A through opening (1011) is formed between the outer shell (101) and the cover plate (107) and communicates the inside and outside of the inner cavity, and the rotating disk (106) partially moves in the through opening (1011); A gear (108) meshing with the periphery of the rotating disk (106); A forward and reverse motor (109), the output shaft of which is fixedly connected to the gear (108) and is fixedly mounted outside the housing (101); Two groups of collection ports (1010) are symmetrically arranged at the top and bottom ends of the housing (101) and are connected to a group of fluorescent filters (105).

2. A fully automatic fluorescence imaging and quantitative analysis microscope according to claim 1, characterized in that: The rotating rod (103) and the rotating disk (106) are detachably fixed by bolts.

3. The fully automatic fluorescence imaging and quantitative analysis microscope according to claim 1, characterized in that: A plurality of groups of clamping blocks (104) are fixedly arranged on the periphery of the rotating rod (103) corresponding to the plurality of groups of fluorescent filters (105); a clamping slot is arranged outside the fluorescent filter (105); the clamping block (104) is detachably fixed to the clamping slot so as to be separated only by sliding up and down; and the bottom end of the clamping block (104) is supported by the top end of the rotating member (102).

4. The fully automatic fluorescence imaging and quantitative analysis microscope according to claim 3, characterized in that: A soft compression layer that fits stably with the card block (104) is provided in the card slot.

5. The fully automatic fluorescence imaging and quantitative analysis microscope according to claim 1, characterized in that: The fluorescent wheel assembly (1) further comprises a sealing patch (1012), which is fixed in the through opening (1011) and fits with the rotating disk (106) to seal the inner cavity.

6. A fully automatic fluorescence imaging and quantitative analysis microscope according to claim 4 or 5, characterized in that: Also includes; A bracket (2), the outer side of which is used for mounting the fluorescent wheel assembly (1); A base (3), the outer side of which is used for mounting the bracket (2); An infinity tube lens (4) fixedly mounted on the top of the housing (101); A scientific camera (5) fixedly mounted on the top of the infinity tube lens (4); A fluorescent light source group (6), a telescopic tube (7), a first objective lens arm (8) and a second objective lens arm (12), all of which are fixedly mounted on the bracket (2); An adjustment seat (11), a backlight source group (14) and a controller (15), all of which are fixedly mounted on the base (3); A loading platform (10) fixedly mounted on an adjustment seat (11); A first objective lens (9) fixedly mounted on the bottom end of the first objective lens arm (8); A second objective lens (13) fixedly mounted on the top of the second objective lens arm (12); The stage (10) is located between the first objective lens (9) and the second objective lens (13); the bottom end of the telescopic tube (7) is fixedly mounted to the first objective lens arm (8); and the collection port (1010), the fluorescent filter (105), the telescopic tube (7), the first objective lens (9), the second objective lens (13) and the backlight source group (14) are sequentially located below the infinity tube lens (4).

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