Incubation device for full-automatic western blot antibody incubation washing system

Abstract

The utility model relates to the technical field of biology, and particularly discloses an incubation device for a full-automatic western blot antibody incubation washing system, which comprises a plurality of incubation boxes, a shaking table tray, a shaking table fixing seat, a shaking table rotating shaft, a shaking table left support arm and a shaking table right support arm, the number of the shaking table fixing seats is two, the two shaking table fixing seats are fixedly connected with the shaking table tray and located at the lower end of the shaking table tray, the shaking table rotating shaft penetrates through the two shaking table fixing seats and is embedded in the two shaking table fixing seats, and the shaking table left supporting arm is arranged at one end of the shaking table rotating shaft. The shaking table right support arm is arranged at one end, far away from the shaking table left support arm, of the shaking table rotating shaft. The waste of labor and time can be reduced, and the test efficiency is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of biology, in particular to an incubation device for a full-automatic western blot antibody incubation and washing system. Background Art

[0002] Currently, there are many experiments in protein-related laboratories that involve immune responses of various antibodies. In the experiments, specific antibodies need to come into contact with membrane supports carrying the experimenter's specific proteins to react.

[0003] However, in the prior art, the reaction process involves sequential reactions of antibodies and multiple washing steps, and the experiment requires many repeated operations and takes a long time, resulting in a waste of a lot of manpower and time, and greatly reducing the experimental efficiency. Utility Model Content

[0004] The utility model aims to provide an incubation device for a fully automatic western blot antibody incubation and washing system, aiming to solve the technical problems in the prior art of involving sequential reactions of antibodies and multiple washing steps in the reaction process, many repeated experimental operations, and a long time required, resulting in a waste of a large amount of manpower and time, and a significant reduction in experimental efficiency.

[0005] To achieve the above-mentioned purpose, the utility model adopts an incubation device for a fully automatic western blot antibody incubation and washing system, comprising an incubation box, a shaker tray, a shaker fixed seat, a shaker shaft, a shaker left arm and a shaker right arm, wherein the number of the incubation boxes is multiple, and each of the incubation boxes is respectively arranged at the upper end of the shaker tray, the number of the shaker fixed seats is two, the two shaker fixed seats are respectively fixedly connected to the shaker tray, and are respectively located at the lower end of the shaker tray, the shaker shaft passes through the two shaker fixed seats, and the shaker shaft is embedded in the interior of the two shaker fixed seats, the shaker left arm is arranged at one end of the shaker shaft, and the shaker right arm is arranged at an end of the shaker shaft away from the shaker left arm.

[0006] Among them, each of the incubation boxes has multiple front columns, multiple rear columns and multiple staples, and the multiple front columns and multiple rear columns are respectively arranged inside the incubation box, the multiple staples are respectively arranged at the lower end of the incubation box, and the multiple staples are adapted to the shaker tray, one side of the incubation box has a wedge-shaped mouth, and the bottom of the incubation box is an arc structure.

[0007] Among them, each of the rocking table fixing seats includes a seat body, a fixing bolt, a clamping bolt and a clamping nut. The seat body is arranged on one side of the rocking table tray. The number of the fixing bolts is multiple, each of the fixing bolts passes through the seat body and is threadedly connected to the rocking table tray, and the seat body is sleeved on the outer wall of the rocking table rotating shaft, one end of the clamping bolt passes through the seat body and is threadedly connected to the clamping nut, and the clamping nut is sleeved on the outer wall of the clamping bolt.

[0008] Among them, the left arm of the rocking table includes a left mounting plate, a left deep groove ball bearing, a left rocking table photoelectric seat and a left cover bin, the left deep groove ball bearing is sleeved on the outer wall of the rocking table bearing, the left mounting plate is sleeved on the outer wall of the left deep groove ball bearing, the left rocking table photoelectric seat is fixedly connected to the left mounting plate and is located on one side of the left mounting plate, the left cover bin is fixedly connected to the left mounting plate and is located on one side of the left mounting plate, and the left cover bin covers the left rocking table photoelectric seat.

[0009] Among them, the right support arm of the rocking table includes a right mounting plate, a right deep groove ball bearing, a right rocking table photoelectric seat and a right cover bin, the right deep groove ball bearing is sleeved on the outer wall of the rocking table bearing, the right mounting plate is sleeved on the outer wall of the right deep groove ball bearing, the right rocking table photoelectric seat is fixedly connected to the right mounting plate and is located on one side of the right mounting plate, the right cover bin is fixedly connected to the right mounting plate and is located on one side of the right mounting plate, and the right cover bin covers the right rocking table photoelectric seat.

[0010] Among them, the incubation device for the fully automatic western blot antibody incubation and washing system also includes an elastic retaining ring for the shaft, the number of the elastic retaining rings for the shaft is two, the two elastic retaining rings for the shaft are respectively sleeved on the outer wall of the shaking table rotating shaft, and the two elastic retaining rings for the shaft are respectively located on one side of the corresponding left deep groove ball bearing and the right deep groove ball bearing.

[0011] The incubation device for a fully automatic western blot antibody incubation and washing system of the utility model has the following beneficial effects: the arc setting of the bottom of the incubation box allows the sample film to have a certain tension and to be supported to be close to the arc-shaped bottom of the box; when the incubation box is shaken with the shaker, the sample film is always deposited on the bottom of the reagent and will not move with the reagent, nor will it float on the reagent liquid surface, so that the sample on the film and the reagent fully react; the front column and the rear column are perpendicular to the incubation box and can block the sample film, so that the sample film slides on the bottom of the incubation box and will not exceed the columns, thereby ensuring that the reagent can submerge the sample film, reducing the waste of labor and time, and improving the test efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0013] Figure 1 The utility model is a structural schematic diagram of an incubation device for a fully automatic western blot antibody incubation and washing system.

[0014] Figure 2 It is a side view of an incubation device for a fully automatic western blot antibody incubation and washing system of the utility model.

[0015] Figure 3 The utility model is a top view of an incubation device for a fully automatic western blot antibody incubation and washing system.

[0016] Figure 4 The utility model is a cross-sectional view of the internal structure of an incubation device for a fully automatic western blot antibody incubation and washing system.

[0017] 1-incubation box, 2-shaker tray, 3-seat, 4-fixing bolt, 5-clamping bolt, 6-clamping nut, 7-shaker shaft, 8-left mounting plate, 9-left deep groove ball bearing, 10-left shaker photoelectric seat, 11-left cover, 12-right mounting plate, 13-right deep groove ball bearing, 14-right shaker photoelectric seat, 15-right cover, 16-rear column, 17-front column, 18-pin, 19-wedge-shaped mouth, 20-elastic retaining ring for shaft. DETAILED DESCRIPTION

[0018] See also Figures 1 to 4 The utility model provides an incubation device for a fully automatic western blot antibody incubation and washing system, comprising an incubation box 1, a rocking table tray 2, a rocking table fixing seat, a rocking table shaft 7, a rocking table left arm and a rocking table right arm, wherein the number of the incubation boxes 1 is multiple, and each of the incubation boxes 1 is respectively arranged at the upper end of the rocking table tray 2, the number of the rocking table fixing seats is two, and the two rocking table fixing seats are respectively fixedly connected to the rocking table tray 2 and are respectively located at the lower end of the rocking table tray 2, the rocking table shaft 7 passes through the two rocking table fixing seats, and the rocking table shaft 7 is embedded in the two rocking table fixing seats, the rocking table left arm is arranged at one end of the rocking table shaft 7, and the rocking table right arm is arranged at one end of the rocking table shaft 7 away from the rocking table left arm.

[0019] Furthermore, each of the incubation boxes 1 has a plurality of front columns 17, a plurality of rear columns 16 and a plurality of staples 18, and the plurality of front columns 17 and the plurality of rear columns 16 are respectively arranged inside the incubation box 1, the plurality of staples 18 are respectively arranged at the lower end of the incubation box 1, and the plurality of staples 18 are adapted to the shaker tray 2, one side of the incubation box 1 has a wedge-shaped mouth 19, and the bottom of the incubation box 1 is an arc structure.

[0020] Furthermore, each of the rocking table fixing seats includes a seat body 3, a fixing bolt 4, a clamping bolt 5 and a clamping nut 6, the seat body 3 is arranged on one side of the rocking table tray 2, the fixing bolts 4 are multiple in number, each of the fixing bolts 4 passes through the seat body 3 and is threadedly connected to the rocking table tray 2, and the seat body 3 is sleeved on the outer wall of the rocking table rotating shaft 7, one end of the clamping bolt 5 passes through the seat body 3 and is threadedly connected to the clamping nut 6, and the clamping nut 6 is sleeved on the outer wall of the clamping bolt 5.

[0021] Furthermore, the left arm of the rocking table includes a left mounting plate 8, a left deep groove ball bearing 9, a left rocking table photoelectric seat 10 and a left cover bin 11. The left deep groove ball bearing 9 is sleeved on the outer wall of the rocking table bearing, and the left mounting plate 8 is sleeved on the outer wall of the left deep groove ball bearing 9. The left rocking table photoelectric seat 10 is fixedly connected to the left mounting plate 8 and is located on one side of the left mounting plate 8. The left cover bin 11 is fixedly connected to the left mounting plate 8 and is located on one side of the left mounting plate 8, and the left cover bin 11 covers the left rocking table photoelectric seat 10.

[0022] Furthermore, the right arm of the rocking table includes a right mounting plate 12, a right deep groove ball bearing 13, a right rocking table photoelectric seat 14 and a right cover bin 15, the right deep groove ball bearing 13 is sleeved on the outer wall of the rocking table bearing, the right mounting plate 12 is sleeved on the outer wall of the right deep groove ball bearing 13, the right rocking table photoelectric seat 14 is fixedly connected to the right mounting plate 12 and is located on one side of the right mounting plate 12, the right cover bin 15 is fixedly connected to the right mounting plate 12 and is located on one side of the right mounting plate 12, and the right cover bin 15 covers the right rocking table photoelectric seat 14.

[0023] Furthermore, the incubation device for the fully automatic western blot antibody incubation and washing system also includes an elastic retaining ring 20 for the shaft, the number of the elastic retaining rings 20 for the shaft is two, the two elastic retaining rings 20 for the shaft are respectively sleeved on the outer wall of the shaking table shaft 7, and the two elastic retaining rings 20 for the shaft are respectively located on one side of the corresponding left deep groove ball bearing 9 and the right deep groove ball bearing 13.

[0024] In this embodiment, the rocking table tray 2 drives the incubation box 1 to do a seesaw motion. The sample film has a certain tension and can be close to the bottom of the incubation box 1. By utilizing a small amount of reagent at the bottom of the incubation box 1 and the seesaw motion of the incubation box 1, the reaction reagent is forced to move back and forth inside the incubation box 1, so that the sample film and the reagent are fully reacted. The rocking table tray 2 is clamped and fixed by the staples 18. At the same time, the rocking table tray 2 can be installed with 6 incubation boxes 1 of 25mm size or 6 incubation boxes 1 of 50mm size or 6 incubation boxes 1 of 60mm size or 3 incubation boxes 1 of 90mm size or 2 incubation boxes 1 of 160mm size through the staples 18, so as to adapt to sample films of different sizes. The front column 17 and the rear column 16 limit the sample film when the incubation box 1 is subjected to the seesaw motion, and the seat 3 is installed and fixed by the fixing bolts 4. The seat body 3 is sleeved on the outer wall of the rocking table shaft 7 and is locked by the threaded connection of the clamping bolt 5 and the clamping nut 6, so that the seat body 3 is fixed to the outer wall of the rocking table shaft 7, the left deep groove ball bearing 9 is installed on the left mounting plate 8, and the right deep groove ball bearing 13 is installed on the right mounting plate 12, so as to facilitate the seesaw movement of the rocking table tray 2, the left mounting plate 8 supports the left rocking table photoelectric seat 10, and the left cover warehouse 11 covers the left rocking table photoelectric seat 10, the right mounting plate 12 supports the right rocking table photoelectric seat 14, and the right cover warehouse 15 covers the right rocking table photoelectric seat 14, the seesaw movement of the rocking table tray 2 is controlled by the left rocking table photoelectric seat 10 and the right rocking table photoelectric seat 14, the shaft elastic retaining ring 20 is used to prevent the right deep groove ball bearing 13 and the left deep groove ball bearing 9 from sliding, thereby reducing the vibration and noise generated by the equipment.

[0025] The above disclosure is only a preferred embodiment of the present invention, and certainly cannot be used to limit the scope of rights of the present invention. Ordinary technicians in this field can understand that all or part of the processes of the above embodiment and equivalent changes made according to the claims of the present invention still fall within the scope covered by the utility model.

Claims

1. An incubation device for a fully automatic western blot antibody incubation and washing system, characterized in that: It includes an incubation box, a shaker tray, a shaker fixing seat, a shaker shaft, a shaker left arm and a shaker right arm. There are multiple incubation boxes, each of which is respectively arranged at the upper end of the shaker tray. There are two shaker fixing seats, which are respectively fixedly connected to the shaker tray and are respectively located at the lower end of the shaker tray. The shaker shaft passes through the two shaker fixing seats, and the shaker shaft is embedded in the two shaker fixing seats. The shaker left arm is arranged at one end of the shaker shaft, and the shaker right arm is arranged at one end of the shaker shaft away from the shaker left arm.

2. An incubation device for a fully automatic western blot antibody incubation and washing system as claimed in claim 1, characterized in that: Each of the incubation boxes has multiple front columns, multiple rear columns and multiple staples, and the multiple front columns and multiple rear columns are respectively arranged inside the incubation box, the multiple staples are respectively arranged at the lower end of the incubation box, and the multiple staples are adapted to the shaker tray, one side of the incubation box has a wedge-shaped mouth, and the bottom of the incubation box is an arc structure.

3. An incubation device for a fully automatic western blot antibody incubation and washing system as claimed in claim 2, characterized in that: Each of the rocking table fixing seats includes a seat body, a fixing bolt, a clamping bolt and a clamping nut. The seat body is arranged on one side of the rocking table tray. There are multiple fixing bolts. Each fixing bolt passes through the seat body and is threadedly connected to the rocking table tray. The seat body is sleeved on the outer wall of the rocking table rotating shaft. One end of the clamping bolt passes through the seat body and is threadedly connected to the clamping nut. The clamping nut is sleeved on the outer wall of the clamping bolt.

4. An incubation device for a fully automatic western blot antibody incubation and washing system as claimed in claim 3, characterized in that: The left support arm of the rocking table includes a left mounting plate, a left deep groove ball bearing, a left rocking table photoelectric seat and a left cover bin. The left deep groove ball bearing is sleeved on the outer wall of the rocking table bearing, and the left mounting plate is sleeved on the outer wall of the left deep groove ball bearing. The left rocking table photoelectric seat is fixedly connected to the left mounting plate and is located on one side of the left mounting plate. The left cover bin is fixedly connected to the left mounting plate and is located on one side of the left mounting plate, and the left cover bin covers the left rocking table photoelectric seat.

5. An incubation device for a fully automatic western blot antibody incubation and washing system as claimed in claim 4, characterized in that: The right support arm of the rocking table includes a right mounting plate, a right deep groove ball bearing, a right rocking table photoelectric seat and a right cover bin. The right deep groove ball bearing is sleeved on the outer wall of the rocking table bearing, and the right mounting plate is sleeved on the outer wall of the right deep groove ball bearing. The right rocking table photoelectric seat is fixedly connected to the right mounting plate and is located on one side of the right mounting plate. The right cover bin is fixedly connected to the right mounting plate and is located on one side of the right mounting plate, and the right cover bin covers the right rocking table photoelectric seat.

6. An incubation device for a fully automatic western blot antibody incubation and washing system as claimed in claim 5, characterized in that: The incubation device for the fully automatic western blot antibody incubation and washing system also includes a shaft elastic retaining ring, the number of the shaft elastic retaining rings is two, the two shaft elastic retaining rings are respectively sleeved on the outer surface wall of the shaking table rotating shaft, and the two shaft elastic retaining rings are respectively located on one side of the corresponding left deep groove ball bearing and the right deep groove ball bearing.

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