Nucleic acid extraction and detection analyzer

Abstract

The utility model provides a nucleic acid extraction and detection analyzer which comprises a base module used for carrying a kit and driving the kit to reciprocate between a delivery position and a detection position along the Y direction; the extraction module is mounted above the base module, the extraction module comprises a lifting mechanism, a rotating mechanism, a pipette pump used for sucking pipette tips and a magnetic attraction assembly used for magnetically attracting magnetic beads, the magnetic attraction assembly and the pipette pump are both connected with the lifting mechanism, and the rotating mechanism is used for driving an upper shell of a kit to rotate in a horizontal plane; the magnetic beads are transferred in different chambers in the kit; the detection module is mounted at the front end of the base module along the Y direction, and the detection module is used for detecting a solution in the kit. According to the utility model, the magnetic attraction assembly is arranged on the extraction module at the upper part to form an upper attraction mode, so that the magnetic beads can be conveniently transferred in the kit, the high infection risk caused by liquid transfer in the prior art can be avoided, and the detection precision is relatively high.

Description

Technical Field

[0001] This utility model belongs to the field of detection instrument technology, and in particular relates to a nucleic acid extraction and detection analyzer. Background Technology

[0002] Molecular diagnostics is the foundation of precision medicine and the fastest-growing subfield of in vitro diagnostics. Its applications are extremely broad, including the detection and diagnosis of genetic diseases, infectious diseases, and tumors. Point-of-care testing (POCT) using molecular diagnostic methods, with its advantages of high sensitivity and accuracy, portability, ease of operation, short testing time, no environmental requirements, and low cost, is highly likely to become the mainstream of the future in vitro diagnostics industry. For example, existing molecular POCT systems like Iponatic require a separate external centrifuge and manual transfer of reaction tubes to complete the entire testing process.

[0003] Currently, some fully automated testing instruments have been launched on the market. These instruments are all large-scale instruments, and the process is still the same as the traditional method, which is relatively time-consuming. The test results cannot be released in time, making it difficult to promote and use them widely at the grassroots level. Secondly, some POCT molecular diagnostic instruments on the market are intended to achieve the ideal model of sample in and result out, but in clinical practice, they often cannot achieve a truly closed testing, and still have a high risk of infection. Utility Model Content

[0004] The main objective of this invention is to propose a nucleic acid extraction and detection analyzer, which aims to solve the technical problem of high infection risk in existing nucleic acid extraction and detection analyzers.

[0005] To achieve the above objectives, this utility model provides a nucleic acid extraction and detection analyzer for a reagent kit pre-loaded with pipette tips and magnetic beads. The nucleic acid extraction and detection analyzer includes: a base module for carrying the reagent kit and driving it to reciprocate between an outlet position and a detection position along the Y direction; an extraction module installed above the base module, the extraction module including a lifting mechanism, a rotating mechanism, a pipetting pump for aspirating the pipette tips, and a magnetic suction assembly for magnetically attracting the magnetic beads. The magnetic suction assembly and the pipetting pump are both connected to the lifting mechanism. The rotating mechanism drives the upper shell of the reagent kit to rotate in a horizontal plane and transfers the magnetic beads between different chambers within the reagent kit; and a detection module installed at the front end of the base module along the Y direction, the detection module being used to detect the solution within the reagent kit.

[0006] In this embodiment of the utility model, the lifting mechanism includes: a first driving member; the magnetic suction assembly includes a magnetic rod for magnetically attracting the magnetic bead and an insulating sleeve sleeved outside the magnetic rod; the first driving member is connected to the magnetic rod; there are two first driving members, one of which is connected to the magnetic rod and the other is connected to the insulating sleeve; and a second driving member is connected to the pipette pump and used to drive the pipette pump to lift and lower.

[0007] In this embodiment of the present invention, the output ends of both first driving components are connected to magnetic suction rods, the magnetic rod is connected to a first slider, the first slider and one of the magnetic suction rods are threaded together, the insulating sleeve is connected to a second slider, the second slider and the other magnetic suction rod are threaded together.

[0008] In this embodiment of the present invention, the two magnetic suction rods are spaced apart along the Y direction, and the first slider and the second slider are both arranged between the two magnetic suction rods along the Y direction, with the first slider located above the second slider; and / or, the lifting mechanism further includes a first guide rod, which passes through the first slider and the second slider, and the first slider and the second slider are guided and cooperated with the first guide rod.

[0009] In this embodiment of the invention, the pipette pump is connected to a pipette slider, and the output end of the second drive is connected to a pipette screw. The pipette slider and the pipette screw are threaded together.

[0010] In this embodiment of the invention, the reagent kit is further provided with a pipetting plunger, and the lifting mechanism further includes a plunger driving assembly and a push block connected to the pipetting pump. The push block can press down the pipetting plunger through the plunger driving assembly under the drive of the pipetting pump; and / or, the lifting mechanism further includes a second guide rod that guides and cooperates with the pipetting slider.

[0011] In this embodiment of the utility model, the plunger drive assembly includes: a fixed rod, a toothed sleeve movably sleeved on the fixed rod, and a pushing protrusion on the toothed sleeve for abutting against the pushing block; a pressing piston for pressing down the pipetting plunger; and a pipetting gear, in which the toothed sleeve and the pressing piston mesh with the pipetting gear.

[0012] In this embodiment of the utility model, the pushing block is provided with a relief groove into which the pushing protrusion extends, and the bottom wall of the relief groove is provided with a pushing plane for contacting the surface of the pushing block; and / or, the lifting mechanism further includes a third guide rod that guides and cooperates with the pushing block.

[0013] In this embodiment of the utility model, the nucleic acid extraction and detection analyzer further includes an upper mounting base, a vertical plate, and a lower mounting base connected to the rotating mechanism. The vertical plate extends along the Z direction and is supported between the upper mounting base and the lower mounting base. The first driving member and the second driving member are both mounted on the top of the upper mounting base. The upper mounting base has an upper clearance hole to avoid the pipette pump, and the lower mounting base has a lower clearance hole for the pipette pump nozzle to pass through.

[0014] In this embodiment of the invention, the rotating mechanism includes a rotating drive, a belt, and a pulley. The rotating drive drives the pulley to rotate via the belt. The bottom of the pulley is provided with a rotating protrusion adapted to the reagent kit. And / or, the base module includes a box base, a base drive, a base gear, and a base rack extending along the Y direction. The output end of the base drive is connected to the base gear, the box base is connected to the base rack, and the base gear and the base rack mesh.

[0015] Through the above technical solution, the nucleic acid extraction and detection analyzer provided in this utility model embodiment has the following beneficial effects:

[0016] The extraction module is positioned above the base module, and its rotation mechanism drives the reagent kit for pipetting and rotation. A magnetic suction component is connected to the extraction module above. When performing nucleic acid detection analysis using a nucleic acid extraction and detection analyzer, the base module can move relative to the extraction module to the exit position. At the exit position, the base module is away from the extraction module, allowing operators to easily place the reagent kit inside. The base module can then drive the reagent kit relative to the extraction module to the detection position. The reagent kit at the detection position is located below the extraction module. A lifting mechanism, driving the magnetic suction component to move up and down, combined with the rotation mechanism for pipetting and rotation of the reagent kit, transfers the magnetic beads within the reagent kit to different chambers until nucleic acid extraction is complete. The lifting mechanism can then drive a pipetting pump to transfer the extracted nucleic acid. After nucleic acid processing, the solution within the reagent kit can be detected using the detection module. The nucleic acid extraction and detection analyzer of this utility model, by setting the magnetic suction component on the extraction module above, forms an upper adsorption method, which can facilitate the transfer of magnetic beads in the reagent kit and avoid the high infection risk of liquid transfer in the prior art. Through the cooperation of the upper adsorption and rotation mechanism, the magnetic beads in the reagent kit can be transferred with high detection accuracy. With the joint action of the base module and the extraction module, the transfer and mixing of magnetic beads in the reagent kit can be completed, and the entire nucleic acid extraction process can be completed.

[0017] Other features and advantages of this invention will be described in detail in the following detailed description section. Attached Figure Description

[0018] The accompanying drawings are provided to illustrate the present invention and form part of the specification. They are used together with the following detailed description to explain the present invention, but do not constitute a limitation thereof. In the drawings:

[0019] Figure 1 This is a schematic diagram of the structure of a nucleic acid extraction and detection analyzer according to an embodiment of the present invention;

[0020] Figure 2 This is a schematic diagram of the extraction module of the nucleic acid extraction and detection analyzer according to an embodiment of the present invention from one perspective;

[0021] Figure 3 This is a schematic diagram of the extraction module of the nucleic acid extraction and detection analyzer according to one embodiment of the present invention from another perspective;

[0022] Figure 4 This is a schematic diagram of the extraction module of the nucleic acid extraction and detection analyzer according to an embodiment of the present invention from another perspective.

[0023] Explanation of reference numerals in the attached figures

[0024] Label Name Label Name

[0025] 100 Nucleic Acid Extraction and Detection Analyzer; 221 Rotary Drive Component

[0026] 1. Base module 222. Belt

[0027] 11 boxes, base, 223 pulleys

[0028] 12 Base drive component 224 Rotating protrusion

[0029] 13 Base gear 23 Pipette pump

[0030] 14 Base rack 231 Pipette slider

[0031] 2 Extraction module 232 Push block

[0032] 21 Lifting mechanism 233 Clearance slot

[0033] 211 First driving component 24 Magnetic suction assembly

[0034] 212 Magnetic Screw 241 Magnetic Rod

[0035] 213 Second driving component 242 First slider

[0036] 214 Pipette screw; 243 Insulating sleeve

[0037] 215 First guide rod 244 Second slider

[0038] 216 Second guide rod 25 Upper mounting base

[0039] 217 Fixed rod 251 Upper clearance hole

[0040] 218 Gear Sleeve 26 Vertical Plate

[0041] 2181 Push-up protrusion 27 Lower mounting base

[0042] 219 Downward-pressing piston; 271 Downward-moving clearance hole

[0043] 210 Pipette Gear 3 Detection Module

[0044] 2101 Third guide rod 200 Reagent kit

[0045] 22 Rotating mechanism Detailed Implementation

[0046] The specific embodiments of this utility model will be described in detail below with reference to the accompanying drawings. It should be understood that the specific embodiments described herein are for illustration and explanation only and are not intended to limit the scope of this utility model.

[0047] The nucleic acid extraction and detection analyzer according to the present invention is described below with reference to the accompanying drawings.

[0048] like Figures 1 to 4 As shown in the embodiment of this utility model, the nucleic acid extraction and detection analyzer 100 is used for a reagent kit 200 pre-loaded with pipette tips and magnetic beads. The nucleic acid extraction and detection analyzer 100 includes a base module 1, an extraction module 2, and a detection module 3. The base module 1 is used to carry the reagent kit 200 and drive the reagent kit 200 to reciprocate between the outlet position and the detection position along the Y direction. The extraction module 2 is installed above the base module 1. The extraction module 2 includes a lifting mechanism 21, a rotating mechanism 22, a pipetting pump 23 for aspirating pipette tips, and a magnetic suction assembly 24 for magnetically attracting magnetic beads. The magnetic suction assembly 24 and the pipetting pump 23 are both connected to the lifting mechanism 21. The rotating mechanism 22 is used to drive the upper shell of the reagent kit 200 to rotate in the horizontal plane and to transfer the magnetic beads in different chambers within the reagent kit 200. The detection module 3 is installed at the front end of the base module 1 along the Y direction and is used to detect the solution within the reagent kit 200.

[0049] Understandably, the detection module 3 in this embodiment can be an existing PCR module. The PCR module may include an existing amplification heating module and an optical detection module. The nucleic acid stored in the reagent kit 200 can be amplified and optically detected in the detection module 3. The reagent kit 200 can adopt the existing structure of a nucleic acid detection reagent kit 200. The reagent kit 200 includes an upper shell and a bottom shell. The bottom shell is provided with multiple independent reagent cartridges, which contain nucleic acid detection reagents and magnetic beads. The upper shell and the bottom shell are rotatably connected. The upper shell is provided with a magnetic suction tube, an upper pipette tip tube for accommodating pipette tips, a plunger tube for accommodating pipette plungers, and a sample cartridge for adding sample solution. The magnetic suction tube is used to accommodate the magnetic suction assembly 24, and the bottom of the magnetic suction tube is sealed while the top is open. A membrane is used to seal between the upper shell and the bottom shell. By setting a magnetic suction tube with a sealed bottom and an open top to accommodate the magnetic suction assembly 24, and by rotatably connecting the upper shell and the bottom shell, nucleic acid can be transferred through magnetic beads during lysis and purification, and inhibitors can be removed as much as possible.

[0050] In this embodiment, the extraction module 2 is positioned above the base module 1, and the rotation mechanism 22 of the extraction module 2 drives the reagent kit 200 to rotate and pipette. The magnetic suction component 24 is connected to the extraction module 2 located above. When performing nucleic acid detection analysis using the nucleic acid extraction and detection analyzer 100, the base module 1 can move relative to the extraction module 2 to the outlet position. At the outlet position, the base module 1 is away from the extraction module 2, allowing the operator to easily place the reagent kit 200 inside the base module 1. The base module 1 can then drive the reagent kit 200 relative to the extraction module 2 to the detection position. At the detection position, the reagent kit 200 is located below the extraction module 2. The lifting mechanism 21, by driving the magnetic suction component 24 to move up and down, in conjunction with the rotation mechanism 22 to rotate the reagent kit 200, can transfer the magnetic beads within the reagent kit 200 to different chambers within the reagent kit 200 until nucleic acid extraction is complete. The lifting mechanism 21 can then drive the pipetting pump 23 to transfer the extracted nucleic acid. After nucleic acid processing, the solution within the reagent kit 200 can be detected by the detection module 3. The nucleic acid extraction and detection analyzer 100 in this embodiment, by setting the magnetic suction component 24 on the upper extraction module 2 to form an upper adsorption method, can facilitate the transfer of magnetic beads in the reagent kit 200 and avoid the high infection risk of liquid transfer in the prior art. In this embodiment, the magnetic beads in the reagent kit 200 can be transferred by the upper adsorption and rotation mechanism 22 in combination, and the detection accuracy is high. With the joint action of the base module 1 and the extraction module 2, the transfer and mixing of magnetic beads in the reagent kit 200 can be completed, and the steps of the entire nucleic acid extraction process can be completed.

[0051] Specifically, the magnetic bead transfer method is as follows: After injecting the sample solution through the sample cylinder, the membrane can be punctured to connect the sample cylinder with the reagent cylinder. At this time, the sample cylinder can be aligned with the reagent cylinder containing the lysis buffer. The sample solution is directly injected into the lysis buffer. The rotating mechanism 22 drives the upper shell to rotate relative to the bottom shell, aligning the magnetic suction cylinder and the reagent cylinder containing the magnetic beads. The lifting mechanism 21 can drive the magnetic suction assembly 24 to descend into the reagent cylinder containing the magnetic beads, allowing the magnetic suction assembly 24 to attract the magnetic beads. After the magnetic suction assembly 24 attracts the magnetic beads, the lifting mechanism 21 can drive the magnetic suction assembly 24 upward. Component 24 is used to attach the magnetic bead to the bottom of the magnetic suction cylinder. The rotating mechanism 22 drives the upper shell to rotate relative to the bottom shell, which can align the magnetic suction cylinder and the reagent cylinder containing the sample liquid. The lifting mechanism 21 can drive the magnetic suction component 24 to descend into the reagent cylinder containing the sample liquid. After the magnetic bead is completely immersed, the lifting mechanism 21 can drive the magnetic suction component 24 to rise until it is demagnetized, so that the magnetic bead falls into the reagent cylinder under the action of gravity, thus completing the transfer of the magnetic bead. The steps for transferring the magnetic bead in other chambers are the same as the above steps.

[0052] Specifically, the lifting mechanism 21 includes a first driving member 211 and a second driving member 213. The magnetic suction assembly 24 includes a magnetic rod 241 with magnetic beads and an insulating sleeve 243 sleeved on the magnetic rod 241. The first driving member 211 is connected to the magnetic rod 241. There are two first driving members 211, one of which is connected to the magnetic rod 241 and the other is connected to the insulating sleeve 243. The second driving member 213 is connected to the pipette pump 23 and is used to drive the pipette pump 23 to lift.

[0053] In this embodiment, both the first driving component 211 and the second driving component 213 can be motors. After the second driving component 213 drives the pipetting pump 23 to rise and fall to aspirate the pipette tip, the pipette tip on the pipetting pump 23 can be driven by the second driving component 213 to puncture the membrane, connecting the sample cylinder and the reagent cylinder. The sample solution is then directly injected into the lysis buffer. The rotating mechanism 22 can drive the upper shell to rotate relative to the bottom shell, aligning the magnetic suction cylinder and the reagent cylinder with magnetic beads. The first driving component 211 can drive the magnetic suction assembly 24 to descend into the reagent cylinder with magnetic beads, allowing the magnetic suction assembly 24 to attract the magnetic beads. After the magnetic suction assembly 24 attracts the magnetic beads, the first driving component 211 can drive the magnetic suction assembly 24 upward to attract the magnetic beads to the bottom of the magnetic suction cylinder. The rotating mechanism 22 can drive the upper shell to rotate relative to the bottom shell, aligning the magnetic suction cylinder and the reagent cylinder with sample solution. The lifting mechanism 21 can drive the magnetic suction assembly 24 to descend into the reagent cylinder with sample solution. Inside the cylinder, after the magnetic beads are completely submerged, the first driving component 211 can independently drive the magnetic suction body to rise relative to the insulating sleeve 243, causing the magnetic beads to fall into the reagent cylinder under gravity. The first driving component 211 can drive the insulating sleeve 243 to move up and down repeatedly inside the reagent cylinder containing the magnetic beads, mixing the solution inside the reagent cylinder containing the magnetic beads. This can mix the sample solution, lysis buffer, and magnetic beads. After the sample is fully lysed and the nucleic acid is completely adsorbed by the magnetic beads, the first driving component 211 can drive the insulating sleeve 243 and the magnetic suction body to descend for magnetic bead adsorption. The magnetic beads containing nucleic acid are adsorbed by the magnetic suction body at the bottom of the magnetic suction cylinder. After the rotating mechanism 22 drives the upper shell to rotate relative to the bottom shell, the magnetic suction body and the insulating sleeve 243 of the magnetic suction assembly 24 are driven to move up and down by the first driving component 211. This allows the transfer of magnetic beads in other nucleic acid detection reagents to the purification of the nucleic acid adsorbed on the magnetic beads.

[0054] In this embodiment, the magnetic rod 241 and the insulating sleeve 243 in the magnetic attraction assembly 24 are driven by two first driving members 211, which facilitates the demagnetization of the magnetic bead. Only the magnetic rod 241 needs to be driven to move upward to achieve the demagnetization of the magnetic bead and the magnetic attraction assembly 24. The structure is simple. In addition, the pipette pump 23 is driven by a separate second driving member 213, which facilitates the individual control of the pipette pump 23 and the magnetic attraction assembly 24, avoids the mutual interference between the movements of the various components, and has a simple structure.

[0055] In one embodiment, the output ends of both first driving components 211 are connected to magnetic suction rods 212. A magnetic rod 241 is connected to a first slider 242, which is threadedly engaged with one of the magnetic suction rods 212. An insulating sleeve 243 is connected to a second slider 244, which is threadedly engaged with the other magnetic suction rod 212. In this embodiment, both the magnetic rod 241 and the insulating sleeve 243 employ a screw and slider drive mechanism, ensuring the accuracy of their upward and downward trajectories.

[0056] It should be noted that the two magnetic suction rods 212 are spaced apart along the Y direction, and the first slider 242 and the second slider 244 are both positioned between the two magnetic suction rods 212 along the Y direction, with the first slider 242 located above the second slider 244. Furthermore, the lifting mechanism 21 also includes a first guide rod 215, which passes through the first slider 242 and the second slider 244, and both the first slider 242 and the second slider 244 are guided and engaged with the first guide rod 215. In this embodiment, the Y direction is the front-to-back direction (which can be the width direction of the nucleic acid extraction and detection analyzer 100), the Z direction is the up-down direction (which can be the height direction of the nucleic acid extraction and detection analyzer 100), and the X direction is the left-to-right direction (which can be the width direction of the nucleic acid extraction and detection analyzer 100).

[0057] In this embodiment, both magnetic suction rods 212 are arranged along the Y-axis, and the first slider 242 and the second slider 244 are both arranged along the Y-axis between the two magnetic suction rods 212. The first slider 242 and the second slider 244 are arranged sequentially in the vertical direction, which improves the structural compactness and achieves miniaturization of the nucleic acid extraction and detection analyzer 100. The first guide rod 215 is in the vertical direction, and the number of first guide rods 215 can be set according to actual usage requirements. The first slider 242 and the second slider 244 are in sliding contact with the first guide rod 215, which can simultaneously guide the first slider 242 and the second slider 244.

[0058] In one embodiment, the pipetting pump 23 is connected to a pipetting slider 231, and the output end of the second drive unit 213 is connected to a pipetting screw 214. The pipetting slider 231 and the pipetting screw 214 are threadedly engaged. In this embodiment, the pipetting pump 23 uses a threaded engagement between the pipetting screw 214 and the pipetting slider 231. When the second drive unit 213 drives the pipetting screw 214 to rotate, the pipetting slider 231 can be raised and lowered relative to the pipetting screw 214. The structure is simple and the motion trajectory can be precisely controlled. It should be noted that in one embodiment, the extraction module 2 also includes a detection sensor for detecting whether the raising and lowering is in place. The detection sensor can be a photoelectric sensor. The first slider 242, the second slider 244, and the pipetting slider 231 are all provided with detection baffles that cooperate with the detection sensor. The detection baffles can extend into the detection groove of the detection sensor, so that the detection sensor generates a displacement signal.

[0059] like Figure 4As shown, the reagent kit 200 also includes a pre-installed pipette plunger. The lifting mechanism 21 further includes a plunger drive assembly and a push block 232 connected to the pipette pump 23. The push block 232 can press down the pipette plunger through the plunger drive assembly under the drive of the pipette pump 23. Furthermore, the lifting mechanism 21 also includes a second guide rod 216 that guides and cooperates with the pipette slider 231, and the second guide rod 216 passes through the pipette slider 231. In this embodiment, the pipette plunger can share the same drive component with the pipette pump 23, resulting in a simple structure, eliminating the need for a drive component, and reducing production costs.

[0060] Specifically, the plunger drive assembly includes a fixed rod 217, a pressing piston 219, and a pipetting gear 210. A toothed sleeve 218 is movably sleeved on the fixed rod 217, and the toothed sleeve 218 is provided with a pushing protrusion 2181 for abutting against the pushing block 232. The pressing piston 219 is used to press down the pipetting plunger. Both the toothed sleeve 218 and the pressing piston 219 mesh with the pipetting gear 210. When the second drive member 213 drives the pipetting pump 23 to move upward, the pushing block 232 rises with the pipetting pump 23 and pushes the pushing protrusion 2181 upward. The toothed sleeve 218 can move upward relative to the fixed rod 217 and meshes with the pipetting gear 210, which can drive the pipetting gear 210 to rotate. At the same time, the pressing piston 219 meshes with the pipetting gear 210, which can drive the pressing piston 219 to press down the pipetting plunger. In this embodiment, the gear and rack structure is used to achieve the downward pressing of the pipetting plunger when the second driving component 213 drives the pipetting pump 23 to rise, thus avoiding mutual interference between the pipetting pump 23 and the pipetting plunger.

[0061] It should be noted that the push block 232 is provided with a relief groove 233 for the push protrusion 2181 to extend into, and the bottom wall of the relief groove 233 is provided with a push surface for contacting the surface of the push block 232; furthermore, the lifting mechanism 21 also includes a third guide rod 2101 that guides and cooperates with the push block 232. In this embodiment, the accommodating size of the relief groove 233 can be larger than the size of the push protrusion 2181, which facilitates the extension of the push protrusion 2181. In this embodiment, by providing a relief groove 233 on the push block 232, the structural fit between the push block 232 and the push protrusion 2181 can be made more compact. The third guide rod 2101 passes through the push block 232, and during the lifting and lowering process of the push block 232, the third guide rod 2101 and the push block 232 slide in contact.

[0062] In one embodiment, the nucleic acid extraction and detection analyzer 100 further includes an upper mounting base 25, a vertical plate 26, and a lower mounting base 27 connected to the rotating mechanism 22. The vertical plate 26 extends along the Z direction and is supported between the upper mounting base 25 and the lower mounting base 27. The first driving member 211 and the second driving member 213 are both mounted on the top of the upper mounting base 25. The upper mounting base 25 has an upper clearance hole 251 for avoiding the pipette pump 23, and the lower mounting base 27 has a lower clearance hole 271 for the nozzle of the pipette pump 23 to pass through. In this embodiment, both the upper mounting base 25 and the lower mounting base 27 have circular cross-sections. The vertical plate 26 is supported between the upper mounting base 25 and the lower mounting base 27. The upper clearance hole 251 of the upper mounting base 25 allows the pump body of the pipette pump 23 to pass through easily, and the lower clearance hole 271 of the lower mounting base 27 allows the nozzle of the pipette pump 23 to pass through easily. Compared with the structure of the pipette pump 23 passing through the mounting structure as a whole in the prior art, the size of the lower mounting base 27 and the upper mounting base 25 can be reduced, further realizing the miniaturization of the nucleic acid extraction and detection analyzer 100.

[0063] It should be noted that the rotating mechanism 22 includes a rotating drive 221, a belt 222, and a pulley 223. The rotating drive 221 drives the pulley 223 to rotate via the belt 222. The bottom of the pulley 223 is provided with a rotating protrusion 224 that is compatible with the reagent kit 200. Furthermore, the base module 1 includes a box base 11, a base drive 12, a base gear 13, and a base rack 14 extending along the Y direction. The output end of the base drive 12 is connected to the base gear 13, and the box base 11 is connected to the base rack 14. The base gear 13 and the base rack 14 mesh.

[0064] In this embodiment, the rotary drive component 221 and the base drive component 12 can be motors. The rotary mechanism 22 in this embodiment adopts a belt drive structure, which is simple. The base module 1 adopts a gear and rack structure, which eliminates the need for a large drive component and makes the overall size of the base drive component 12 smaller. This reduces the overall width of the nucleic acid extraction and detection analyzer 100, which can meet market demands. Multiple modules can be connected in parallel within a limited space to achieve multi-channel combination.

[0065] In the description of this utility model, it should be understood that the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Therefore, a feature defined as "first" or "second" may explicitly or implicitly include at least one of that feature. In the description of this utility model, "multiple" means at least two, such as two, three, etc., unless otherwise explicitly specified.

[0066] In this utility model, unless otherwise explicitly specified and limited, the terms "installation," "connection," "joining," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection, an electrical connection, or a connection that allows communication between them; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components, unless otherwise explicitly limited. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0067] In the description of this specification, the references to terms such as "one embodiment," "some embodiments," "example," "specific example," or "some examples," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. Moreover, without contradiction, those skilled in the art can combine and integrate the different embodiments or examples described in this specification, as well as the features of different embodiments or examples.

[0068] Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention. Those skilled in the art can make changes, modifications, substitutions and variations to the above embodiments within the scope of the present invention.

Claims

1. A nucleic acid extraction and detection analyzer for use with a reagent kit (200) pre-loaded with pipette tips and magnetic beads, characterized in that, The nucleic acid extraction and detection analyzer (100) includes: The base module (1) is used to carry the reagent kit (200) and drive the reagent kit (200) to reciprocate between the outlet position and the detection position along the Y direction; Extraction module (2) is installed above the base module (1). The extraction module (2) includes a lifting mechanism (21), a rotating mechanism (22), a pipetting pump (23) for aspirating the pipette tip, and a magnetic suction assembly (24) for magnetically attracting the magnetic beads. The magnetic suction assembly (24) and the pipetting pump (23) are both connected to the lifting mechanism (21). The rotating mechanism (22) is used to drive the upper shell of the reagent kit (200) to rotate in the horizontal plane and to transfer the magnetic beads in different chambers of the reagent kit (200). The detection module (3) is installed at the front end of the base module (1) along the Y direction. The detection module (3) is used to detect the solution in the reagent kit (200).

2. The nucleic acid extraction and detection analyzer according to claim 1, characterized in that, The lifting mechanism (21) includes: The first driving member (211) and the magnetic attraction assembly (24) include a magnetic rod (241) for attracting the magnetic beads and an insulating sleeve (243) sleeved on the magnetic rod (241). The first driving member (211) is connected to the magnetic rod (241). There are two first driving members (211), one of which is connected to the magnetic rod (241) and the other is connected to the insulating sleeve (243). The second drive unit (213) is connected to the pipette pump (23) and is used to drive the pipette pump (23) to move up and down.

3. The nucleic acid extraction and detection analyzer according to claim 2, characterized in that, The output ends of both first driving units (211) are connected to magnetic suction rods (212). The magnetic rod (241) is connected to a first slider (242). The first slider (242) and one of the magnetic suction rods (212) are threaded together. The insulating sleeve (243) is connected to a second slider (244). The second slider (244) and the other magnetic suction rod (212) are threaded together.

4. The nucleic acid extraction and detection analyzer according to claim 3, characterized in that, The two magnetic suction rods (212) are spaced apart along the Y direction, and the first slider (242) and the second slider (244) are both arranged between the two magnetic suction rods (212) along the Y direction, with the first slider (242) located above the second slider (244). And / or, The lifting mechanism (21) further includes a first guide rod (215), which passes through the first slider (242) and the second slider (244), and both the first slider (242) and the second slider (244) are guided and cooperated with the first guide rod (215).

5. The nucleic acid extraction and detection analyzer according to any one of claims 2 to 4, characterized in that, The pipetting pump (23) is connected to a pipetting slider (231), and the output end of the second drive (213) is connected to a pipetting screw (214). The pipetting slider (231) and the pipetting screw (214) are threaded together.

6. The nucleic acid extraction and detection analyzer according to claim 5, characterized in that, The reagent kit (200) is also pre-installed with a pipetting plunger. The lifting mechanism (21) also includes a plunger driving assembly and a push block (232) connected to the pipetting pump (23). The push block (232) can press down the pipetting plunger through the plunger driving assembly under the drive of the pipetting pump (23). And / or, The lifting mechanism (21) also includes a second guide rod (216) that guides and cooperates with the pipetting slider (231).

7. The nucleic acid extraction and detection analyzer according to claim 6, characterized in that, The plunger drive assembly includes: A fixing rod (217) is provided with a toothed sleeve (218) movably sleeved on the outside of the fixing rod (217), and the toothed sleeve (218) is provided with a pushing protrusion (2181) for abutting against the pushing block (232); A lowering piston (219) is used to press down the pipette plunger; The pipetting gear (210) is engaged with the toothed sleeve (218) and the pressing piston (219).

8. The nucleic acid extraction and detection analyzer according to claim 7, characterized in that, The push block (232) is provided with a relief groove (233) into which the push protrusion (2181) extends, and the bottom wall of the relief groove (233) is provided with a push surface for contacting the surface of the push block (232). And / or, The lifting mechanism (21) also includes a third guide rod (2101) that guides and cooperates with the push block (232).

9. The nucleic acid extraction and detection analyzer according to any one of claims 2 to 4, characterized in that, The nucleic acid extraction and detection analyzer (100) further includes an upper mounting base (25), a vertical plate (26), and a lower mounting base (27) connected to the rotating mechanism (22). The vertical plate (26) extends along the Z direction and is supported between the upper mounting base (25) and the lower mounting base (27). The first driving member (211) and the second driving member (213) are both mounted on the top of the upper mounting base (25). The upper mounting base (25) has an upper clearance hole (251) to avoid the pipette pump (23), and the lower mounting base (27) has a lower clearance hole (271) for the nozzle of the pipette pump (23) to pass through.

10. The nucleic acid extraction and detection analyzer according to any one of claims 1 to 4, characterized in that, The rotating mechanism (22) includes a rotating drive (221), a belt (222) and a pulley (223). The rotating drive (221) drives the pulley (223) to rotate through the belt (222). The bottom of the pulley (223) is provided with a rotating protrusion (224) that is compatible with the reagent kit (200). And / or, The base module (1) includes a box base (11), a base drive (12), a base gear (13), and a base rack (14) extending along the Y direction. The output end of the base drive (12) is connected to the base gear (13), and the box base (11) is connected to the base rack (14). The base gear (13) and the base rack (14) mesh.

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