Cell recovery culture shaking table

Abstract

The utility model discloses a cell resuscitation culture shaking table, which belongs to the technical field of cell resuscitation culture and comprises a box body, a cover plate is rotatably connected onto the box body, a clamping frame is arranged in the box body, a container is clamped in the clamping frame, a shaking device is fixedly connected into the box body, and a rotating device is fixedly connected onto the upper surface of the shaking device. According to the utility model, when the second motor drives the rotating plate to rotate, the rotating block is driven by the movable rod to do constant-speed circular motion in the shaking frame, and in the process, the motion trail of the rotating block is doubly constrained by the first connecting rod and the second connecting rod to form a composite motion trail; the inclined plate periodically deflects along with the linkage effect of the rotating block to drive the clamping frame to form a 15-degree constant inclination angle, and the unique mechanical linkage design enables the culture container to keep a specific inclination angle during rotating motion, so that a stable vortex field is formed in a culture solution, and the mass transfer efficiency of a gas-liquid interface is remarkably improved.

Description

Technical Field

[0001] This invention belongs to the field of cell resuscitation and culture technology, and particularly relates to a cell resuscitation and culture shaker. Background Technology

[0002] Cell resuscitation culture is the process of removing frozen cells from liquid nitrogen or a -80°C freezer, rapidly thawing them, and transferring them to a preheated culture medium to restore their viability and proliferative capacity. After thawing, the cells need to be centrifuged to remove cryoprotectants (such as DMSO), then resuspended in fresh culture medium and inoculated into culture flasks or dishes. They are then placed in a constant temperature incubator at 37°C and 5% CO2 for static culture. If the cells are suspension cells or need to be expanded, they can be cultured in a shaker. The shaker promotes the uniform distribution of nutrients and gas exchange in the culture medium by a constant rotation speed (usually 100-150 rpm), avoiding cell deposition and thus improving cell growth efficiency and survival rate. The entire operation must be performed under strict aseptic conditions, and the cell status must be observed regularly.

[0003] In existing technologies, the single rotation mode of traditional shakers can only drive the culture medium to generate basic reciprocating motion, which is difficult to form effective fluid mixing. Due to the lack of a multi-dimensional dynamic mixing mechanism, a uniform mass transfer environment cannot be established within the culture system, resulting in limited gas-liquid exchange efficiency. Especially in cell culture, as the physicochemical properties of the culture medium change, this simple movement mode is more prone to insufficient mixing, causing uneven distribution of dissolved oxygen and nutrients in certain areas, affecting cell growth and metabolic activity. Compared with the homogenized mixing effect achieved by bioreactors through optimized flow field design, traditional shakers are significantly insufficient in terms of mass transfer efficiency, which to some extent restricts their application performance in high-density culture or virus amplification processes that require strict control.

[0004] Based on this, the present invention designs a cell resuscitation culture shaker to solve the above problems. Utility Model Content

[0005] The purpose of this invention is to address the problem that in the existing technology, the single rotation mode of traditional shakers can only drive the culture medium to generate basic reciprocating motion, making it difficult to form effective fluid mixing. Due to the lack of a multi-dimensional dynamic mixing mechanism, a uniform mass transfer environment cannot be established in the culture system, resulting in limited gas-liquid exchange efficiency. Therefore, a cell resuscitation culture shaker is proposed.

[0006] To achieve the above objectives, the present invention adopts the following technical solution:

[0007] A cell resuscitation and culture shaker includes a box body, a cover plate rotatably connected to the box body, a clamping frame provided inside the box body, a container being clamped inside the clamping frame, a shaking device fixedly connected inside the box body, and a rotating device fixedly connected to the upper surface of the shaking device.

[0008] The shaking device includes a frame and a second motor. The output end of the second motor is fixedly connected to a rotating plate. A movable rod is provided inside the rotating plate. A rotating block is fixedly connected to the movable rod. A first connecting rod is symmetrically connected inside the frame. A second connecting rod is symmetrically installed inside the frame. The second connecting rod is rotatably connected to the rotating block.

[0009] As a further description of the above technical solution:

[0010] The shaking device also includes a shaking frame disposed within the frame, and the shaking frame and the first connecting rod form a rotatable connection.

[0011] As a further description of the above technical solution:

[0012] An inclined plate is fixedly connected to the upper surface of the rotating block, and the inclination angle is 15 degrees.

[0013] As a further description of the above technical solution:

[0014] The rotating device includes four housings and a first motor. The four housings are fixedly connected to the upper surface of the inclined plate. A sliding rod is provided inside the housing, and a return spring is provided outside the sliding rod.

[0015] As a further description of the above technical solution:

[0016] One end of the pull-back spring is fixedly connected to the outside of the slide rod, and the other end of the pull-back spring is fixedly connected to the upper surface of the housing.

[0017] As a further description of the above technical solution:

[0018] The upper surfaces of the four slide bars are fixedly connected to the same connecting plate.

[0019] As a further description of the above technical solution:

[0020] The inner diameter of the sleeve is slightly larger than the cross-section of the slide rod, and the two form a sliding connection fit.

[0021] As a further description of the above technical solution:

[0022] The first motor is connected through the connecting plate, and the output end of the first motor is fixedly connected to the lower surface of the clamping frame.

[0023] In summary, due to the adoption of the above technical solution, the beneficial effects of this utility model are:

[0024] 1. In this utility model, when the second motor drives the rotating plate to rotate, the movable rod drives the rotating block to make uniform circular motion within the rocking frame. During this process, the motion trajectory of the rotating block is constrained by both the first connecting rod and the second connecting rod, forming a composite motion trajectory. At the same time, the inclined plate deflects periodically with the linkage of the rotating block, causing the clamping frame to form a constant tilt angle of 15 degrees. This unique mechanical linkage design enables the culture container to maintain a specific tilt angle during rotation, thereby forming a stable vortex field inside the culture liquid and significantly improving the mass transfer efficiency of the gas-liquid interface.

[0025] 2. In this utility model, the periodic excitation force generated by the first motor during operation is transmitted to the slide bar-shell mechanism through the connecting plate. In this mechanism, the slide bar is elastically constrained by the pull-back spring, forming a high-frequency reciprocating motion within the shell. This precisely designed elastic vibration system, superimposed with the rotational motion, causes the clamping frame and culture container to generate a composite motion mode, which maintains the basic characteristics of rotational motion while superimposing multi-dimensional micro-amplitude vibration, thereby achieving a more thorough mixing effect of the culture medium. This mechanism achieves a mixing enhancement effect similar to ultrasound-assisted mixing through mechanical means, but avoids the risk of cell damage that ultrasound may cause. Attached Figure Description

[0026] Figure 1 This is a three-dimensional structural diagram of a cell resuscitation and culture shaker proposed in this utility model;

[0027] Figure 2 This is a three-dimensional structural diagram of a cell resuscitation and culture shaker holder proposed in this utility model;

[0028] Figure 3 This is a three-dimensional structural schematic diagram of a shaking device for a cell resuscitation and culture shaker proposed in this utility model;

[0029] Figure 4 This invention proposes a cell resuscitation and culture shaker. Figure 2 An enlarged structural diagram of part A in the middle.

[0030] Legend:

[0031] 1. Box body; 2. Cover plate; 3. Clamping frame; 4. Container; 5. Rotating device; 501. Shell; 502. Slide rod; 503. Pull-back spring; 504. Connecting plate; 505. First motor; 6. Shaking device; 601. Frame; 602. Second motor; 603. Rotating plate; 604. Movable rod; 605. Rotating block; 606. First connecting rod; 607. Shaking frame; 608. Second connecting rod; 609. Inclined plate. Detailed Implementation

[0032] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of the present utility model.

[0033] Please see Figures 1-4 ,

[0034] First embodiment:

[0035] This utility model provides a technical solution: a cell resuscitation culture shaker, including a box body 1, a cover plate 2 rotatably connected to the box body 1, a clamping frame 3 provided inside the box body 1, a container 4 clamped inside the clamping frame 3, a shaking device 6 fixedly connected inside the box body 1, and a rotating device 5 fixedly connected to the upper surface of the shaking device 6.

[0036] The shaking device 6 includes a frame 601 and a second motor 602. The output end of the second motor 602 is fixedly connected to a rotating plate 603. The rotating plate 603 is provided with a movable rod 604. A rotating block 605 is fixedly connected to the movable rod 604. The movable rod 604 and the rotating block 605 are rigidly connected to ensure that the rotational power of the second motor 602 is efficiently transmitted to the rotating block 605. The rotating block 605 forms a stable trajectory movement under the rotational constraint within the second connecting rod 608. This precise fit not only ensures the accurate maintenance of the 15-degree tilt angle, but also avoids mechanical loosening during the movement, making the eddy current effect of the culture medium more stable and controllable.

[0037] A first connecting rod 606 is symmetrically connected inside the frame 601, and a second connecting rod 608 is symmetrically installed inside the frame 601. The second connecting rod 608 is rotatably connected inside the rotating block 605.

[0038] Specifically, such as Figure 2-3 As shown, the shaking device 6 also includes a shaking frame 607 disposed inside the frame 601. The shaking frame 607 and the first connecting rod 606 form a rotatable connection. An inclined plate 609 is fixedly connected to the upper surface of the rotating block 605, and the inclination angle is 15 degrees.

[0039] During operation, when the second motor 602 drives the rotating plate 603 to rotate, the movable rod 604 drives the rotating block 605 to make uniform circular motion within the rocking frame 607. During this process, the motion trajectory of the rotating block 605 is subject to the dual constraints of the first connecting rod 606 and the second connecting rod 608, forming a composite motion trajectory. At the same time, the inclined plate 609 is periodically deflected by the linkage effect of the rotating block 605, causing the clamping frame 3 to form a constant tilt angle of 15 degrees. This unique mechanical linkage design enables the culture container 4 to maintain a specific tilt angle during rotation, thereby forming a stable vortex field inside the culture medium and significantly improving the mass transfer efficiency of the gas-liquid interface.

[0040] Second embodiment:

[0041] Specifically, such as Figure 4 As shown, the rotating device 5 includes four housings 501 and a first motor 505. The four housings 501 are fixedly connected to the upper surface of the inclined plate 609. A sliding rod 502 is provided inside the housing 501. When the sliding rod 502 slides inside the housing 501, the elastic reset action of the pull spring 503 forms a high-frequency reciprocating motion. The design that the inner diameter of the housing 501 is slightly larger than that of the sliding rod 502 not only ensures smooth sliding, but also avoids motion swaying through gap control, so that the vibration force transmitted by the first motor 505 is converted into regular micro-amplitude oscillation, which significantly improves the mixing efficiency.

[0042] A pull-back spring 503 is provided on the outer sleeve of the slide rod 502. One end of the pull-back spring 503 is fixedly connected to the outside of the slide rod 502, and the other end of the pull-back spring 503 is fixedly connected to the upper surface of the housing 501. The preload of the pull-back spring 503 and the movement of the slide rod 502 form a dynamic balance. This can quickly absorb the impact vibration of the first motor 505 and achieve instantaneous reset through elastic energy storage. This combination significantly enhances the thoroughness of the shaking without excessively increasing the mechanical load.

[0043] The upper surfaces of the four slide rods 502 are fixedly connected to the same connecting plate 504. The inner diameter of the sleeve 501 is slightly larger than the cross-section of the slide rods 502, and the two form a sliding connection. The first motor 505 is connected through the connecting plate 504, and the output end of the first motor 505 is fixedly connected to the lower surface of the clamping frame 3.

[0044] During operation, the periodic excitation force generated by the first motor 505 is transmitted to the slide bar 502-casing 501 mechanism through the connecting plate 504. In this mechanism, the slide bar 502 is elastically constrained by the pull-back spring 503, forming a high-frequency reciprocating motion within the casing 501. This precisely designed elastic vibration system, superimposed with the rotational motion, causes the clamping frame 3 and the culture container 4 to generate a composite motion mode. It maintains the basic characteristics of rotational motion while superimposing multi-dimensional micro-amplitude vibration, thereby achieving a more thorough mixing effect of the culture medium. This mechanism achieves a mixing enhancement effect similar to ultrasound-assisted mixing through mechanical means, but avoids the risk of cell damage that ultrasound may cause.

[0045] The above description is only a preferred embodiment of the present utility model, but the protection scope of the present utility model is not limited thereto. Any equivalent substitutions or changes made by those skilled in the art within the technical scope disclosed in the present utility model, based on the technical solution and the inventive concept of the present utility model, should be included within the protection scope of the present utility model.

Claims

1. A cell resuscitation and culture shaker, comprising a housing (1), characterized in that, A cover plate (2) is rotatably connected to the box (1), a clamping frame (3) is provided inside the box (1), a container (4) is clamped inside the clamping frame (3), a shaking device (6) is fixedly connected inside the box (1), and a rotating device (5) is fixedly connected to the upper surface of the shaking device (6). The shaking device (6) includes a frame (601) and a second motor (602). The output end of the second motor (602) is fixedly connected to a rotating plate (603). A movable rod (604) is provided inside the rotating plate (603). A rotating block (605) is fixedly connected to the movable rod (604). A first connecting rod (606) is symmetrically connected inside the frame (601). A second connecting rod (608) is symmetrically installed inside the frame (601). The second connecting rod (608) is rotatably connected inside the rotating block (605).

2. The cell resuscitation and culture shaker according to claim 1, characterized in that, The shaking device (6) further includes a shaking frame (607) disposed inside the frame (601), and the shaking frame (607) and the first connecting rod (606) form a rotational connection.

3. The cell resuscitation and culture shaker according to claim 1, characterized in that, The upper surface of the rotating block (605) is fixedly connected to an inclined plate (609) with an inclination angle of 15 degrees.

4. The cell resuscitation and culture shaker according to claim 3, characterized in that, The rotating device (5) includes four housings (501) and a first motor (505). The four housings (501) are fixedly connected to the upper surface of the inclined plate (609). A sliding rod (502) is provided inside the housing (501), and a pull spring (503) is provided outside the sliding rod (502).

5. The cell resuscitation and culture shaker according to claim 4, characterized in that, One end of the pull-back spring (503) is fixedly connected to the outside of the slide rod (502), and the other end of the pull-back spring (503) is fixedly connected to the upper surface of the housing (501).

6. The cell resuscitation and culture shaker according to claim 4, characterized in that, The upper surfaces of the four slide bars (502) are fixedly connected to the same connecting plate (504).

7. The cell resuscitation and culture shaker according to claim 4, characterized in that, The inner diameter of the sleeve (501) is slightly larger than the cross-section of the slide rod (502), and the two form a sliding connection fit.

8. The cell resuscitation and culture shaker according to claim 4, characterized in that, The first motor (505) is connected through the connecting plate (504), and the output end of the first motor (505) is fixedly connected to the lower surface of the clamping frame (3).

Images