48 results about "Isoamoenylin" patented technology

The invention discloses a synthetic method of glabridin with optical purity, which comprises the following steps: by taking (R)-3-acetoxyl-2-(2, 4-dimethoxyphenyl) propanol and 2-isopentenyl resorcinol as raw materials, carrying out Mitsunobu reaction to obtain a key intermediate compound I, then carrying out hydrolysis and iodination to obtain an intermediate compound II, and then carrying out cyclization to obtain the glabridin with optical purity. Reducing and removing a protecting group to obtain glabridin. By introducing the isopentene side chain, the reaction activity of an olefin functional group is reserved, a key site is provided for subsequent cyclization conversion, the precise spatial configuration of the product is ensured, and finally glabridin with optical purity is prepared. The method is short in route, low in cost, mild in reaction condition and suitable for industrial production.

The present disclosure relates to recombinant microorganisms, such as bacteria, e.g., E. coli, engineered to have improved flux and / or yield of the DXP pathway. Cells can be engineered to comprise nucleotide sequences comprising dxs polypeptides (EC 2.2.1.7); dxr polypeptides (EC 1.1.1.267); ispD polypeptides (EC 2.7.7.60); ispE polypeptides (EC 2.7.1.148); ispF polypeptides (EC 4.6.1.12); ispG polypeptides (EC 1.17.7.1 or EC 1.17.7.3); ispH polypeptides (EC 1.17.7.4); and idi polypeptides (EC 5.3.3.2). Cells can be further engineered to express redox polypeptides, e.g., ferredoxins, flavodoxins, and / or flavodoxin / ferredoxin--NADP reductases (EC 1.19.1.1 or EC 1.18.1.2), e.g., to increase the activity of the ispG and / or ispH polypeptides. Some or all of the nucleotide sequences can be heterologous to the cells. The recombinant microorganisms can be used in methods of isoprenoid production.

The invention relates to an isopentenyl-alpha-pyrone dimer Hyperumonates D-F as well as a preparation method and application thereof, and belongs to the field of medical chemistry, the isopentenyl-alpha-pyrone dimer Hyperumonates D-F is obtained by extracting and separating from leaves and branches of Hypericum monogynum for the first time, and the structural formula of the isopentenyl-alpha-pyrone dimer Hyperumonates D-F is D-F. The invention further discloses a preparation method of the isopentenyl-alpha-pyrone dimer Hyperumonates D-F. The isopentenyl-alpha-pyrone dimer Hypericumonates D-F has the advantages that the compounds D-E have remarkable inhibition effects on generation of NO in mouse microglial cells (BV-2 cells) induced by lipopolysaccharide (LPS), the activity of the compounds D-E is higher than that of positive medicine minocycline, and the compounds D-E have application potentials in the field of neuroinflammation medicines, and the invention also relates to a pharmaceutical composition which comprises the isopentenyl-alpha-pyrone dimer Hypericumonates D-F and can be used for preparing medicines for treating neuroinflammation. And pharmaceutically acceptable auxiliary materials, wherein the pharmaceutical composition has the effect of resisting neuroinflammation.

The invention discloses an icaritin derivative, a composition and application, the structural formula of the icaritin derivative is as follows: in the structural formula, R1 is independently selected from hydrogen, hydroxyl and C1-C3 alkoxy; r2 is independently selected from hydrogen, an isopentenyl group, an isoamyl group and a Mannich base; r3 is independently selected from hydrogen, hydroxyl or OR3 ', R3 'is selected from rhamnosyl, rhamnose-(2-> 1)-glucosyl, rhamnose-(3-> 1)-glucosyl, propynyl and triazole groups substituted by alcoholic hydroxyl, benzyl and glycosyl; r4 is independently selected from a hydroxyl group or OR4 ', and R4' is selected from a propynyl group, a glucosyl group, and an alcoholic hydroxyl group, a benzyl group and a glycosyl substituted triazole group; r5 is independently selected from hydrogen, isopentenyl and Mannich base; mannich base is selected from N (CH3) 2 and N (CH2CH3) 2, R6 is independently selected from hydroxyl and OR6 ', and R6' is selected from hydrogen, rhamnosyl, rhamnose-(2-> 1)-xylosyl and rhamnose-(2-> 1)-rhamnosyl.The icaritin derivative provides a new direction for treatment of sarcopenia.

This invention provides a chalcone isopentenyltransferase gene. GiPT16 This invention relates to the GiPT16 protein, amplification primer set, and applications, belonging to the field of biogenetics technology. It is based on *Glycyrrhiza inflata* (GiPT16 protein, amplification primer set, and applications). Glycyrrhiza inflata Using whole-genome sequencing data and a reverse genetics strategy, the key aromatic isopentenyltransferase GiPT16, involved in the isopentenylation modification of chalcone active ingredients, was successfully identified and functionally characterized. This enzyme was confirmed to specifically catalyze the biosynthesis of psoralen and glycyrrhizin C using DMAPP as a donor. It is the first chalcone isopentenyltransferase characterized in *Glycyrrhiza inflata*, filling a gap in the study of key enzymes in the chalcone isopentenylation metabolic pathway of this species and providing important evidence for further elucidating the molecular mechanisms of quality formation in *Glycyrrhiza inflata* medicinal materials.

The invention relates to application of isopentenyl transferase FnPT1, and belongs to the technical field of enzymology. The isopentenyl transferase FnPT1 screened and verified in the invention is different from most of reported flavonoid isopentenyl transferases, the isopentenyl transferase FnPT1 can catalyze the isopentenyl modification reaction of a larger substrate of flavone glycoside, and the isopentenyl transferase FnPT1 has the potential to be applied to the synthetic biology of the flavone glycoside. The important application value is realized.

The invention discloses a method for preparing isopentenyl indole alkaloid Debromolustramine B. The isopentenyl indole alkaloid Debromolustramine B belongs to the technical field of organic synthesis, an o-bromo aniline aldehyde compound is used as an initial raw material, and on the basis of novel chiral p-toluene sulfinyl guidance, cheap copper catalysis amido alpha-position arylation and 3, 3-dimethyl allyl bromide cyclization reaction, the isopentenyl indole alkaloid Debromolustramine B is synthesized, and the isopentenyl indole alkaloid Debromolustramine B is obtained. According to the present invention, the key aryl quaternary carbon chiral center in the isopentenyl indole alkaloid Debomolustramine B is constructed, and the isopentenyl indole alkaloid Debomolustramine B with the butyrylcholine esterase inhibition activity is prepared through the subsequent series of chemical conversion, such that the material basis is provided for the further research of the natural product Debomolustramine B and the analogue thereof. The method has the advantages that the reaction conditions are mild, the cheap metal copper is used as the catalyst, the synthesis route is short, the energy consumption is low, the environmental protection is facilitated, and the large-scale chiral preparation of the isopentenyl indole alkaloid Debromofloutramine B is facilitated; the method also has the characteristics of relatively good atom economy and high product yield.

This invention belongs to the field of synthetic biology and microbial engineering, and discloses a chemical-biological method for preparing the isopentenyl bibenzyl compound 2-isopentenyl-3,3',4',5-tetrahydroxybibenzyl (NPB-1575) which has anti-ischemic stroke activity. The specific disclosed method includes: (1) chemically synthesizing the substrate dihydropiperidine; (2) constructing an engineered Escherichia coli strain based on the isopentenyl utilization pathway, co-expressing hydroxyethylthiazol kinase, isopentenyl phosphokinase, isopentenyl pyrophosphate isomerase and specific isopentenyl transferase, and efficiently synthesizing the isopentenyl donor; (3) using this strain to catalyze dihydropiperidine in whole cells, the yield in a 200L fermenter can reach 2.54g / L, which is nearly 40 times higher than the existing method; (4) obtaining NPB-1575 with a purity >98.5% by macroporous adsorption resin and normal / reversed phase chromatography and recrystallization. This method uses readily available raw materials, involves simple steps, is environmentally friendly, and yields high purity, laying the foundation for the development of NPB-1575 as a new drug for ischemic stroke and showing significant application potential.

The invention discloses an isopentenyl flavonoid compound as well as a preparation method and application thereof, belongs to the technical field of natural medicinal chemistry, and particularly relates to five isopentenyl flavonoid compounds, namely daphne D, (3 ''R)-daphne F, (3'' S)-daphne F, daphne E and daphne H, which are separated from daphne giraldii Nitsche which is a daphne giraldii plant and are subjected to silica gel column chromatography, macroporous resin, ODS column chromatography and HPLC (High Performance Liquid Chromatography), and the inhibitory activity of the five isopentenyl flavonoid compounds on HepG2 and Hep3B cells is tested. Finally, the compound plays an anti-tumor role by inhibiting the growth activity of tumor cells. The preparation method disclosed by the invention is simple and good in reproducibility, and the obtained compound is high in purity and has obvious anti-tumor activity. # imgabs0 #

The application discloses a process for preparing 3-methyl-2-buten-1-al diisopentyl acetal by continuous reaction rectification. The process comprises the following steps: reacting and rectifying isopentenyl alcohol and isopentenyl aldehyde in a reaction rectification tower to prepare 3-methyl-2-buten-1-al diisopentyl acetal, characterized in that the content of 2-methyl-3-buten-2-al in the liquid phase of the third to fifth trays of the reaction rectification tower from bottom to top is less than or equal to 1 wt%. By the continuous reaction rectification process, the device can be stably operated for more than 7200 hours, and the economic benefit of the enterprise can be remarkably improved.

The invention belongs to the technical field of enzyme engineering, and discloses a high-activity isopentenyl phosphokinase mutant and application thereof. The high-activity isopentenyl phosphokinase mutant is AtIPKH274P, and the amino acid at the 274 site of wild type AtIPK is mutated from histidine H to proline P. The invention further discloses a preparation method of the high-activity isopentenyl phosphokinase mutant. According to the present invention, the AtIPKH274P, the SmDAGKS47A and the L124A can form the efficient isopentenol utilization pathway so as to effectively convert the prenol into the DMAPP; an engineering strain with improved isopentenyl modification capability can be constructed by utilizing the approach, and the strain has more advantages compared with a traditional engineering strain based on a mevalonic acid approach.

The present invention discloses a method for synthesizing optically pure glabridin. The method uses (R)-3-acetoxy-2-(2,4-dimethoxyphenyl)propanol and 2-isopentenylresorcinol as raw materials to obtain a key intermediate compound I through a Mitsunobu reaction, and then obtains an intermediate compound II through hydrolysis and iodination. The intermediate compound II is then cyclized, reduced, and protected by removal to obtain glabridin. The present invention retains the reactivity of the olefin functional group by introducing an isopentyl side chain, provides a key site for subsequent cyclization, ensures the precise stereoconfiguration of the product, and ultimately obtains optically pure glabridin. The present invention has a short route, low cost, mild reaction conditions, and is suitable for industrial production.

The application provides a fusariobutenolide derivative 4''-O-isopentenyl fusariobutenolide C, and a structural formula of the compound is provided.The 4''-O-isopentenyl fusariobutenolide C can significantly inhibit the growth of seven human routine pathogenic bacteria in antibacterial activity testing, and the MIC is 8.6-28.3 μg / mL, and the antibacterial effect on one strain of human drug-resistant pathogenic bacteria, methicillin-resistant Staphylococcus aureus, is significant, and the MIC is 16.8 μg / mL, and the 4''-O-isopentenyl fusariobutenolide C can be applied to preparation of antibacterial drug precursors, and lays a material foundation for next-step biological medicine development and application of antibacterial components.

The invention discloses a preparation method of all-trans-farnesol through a three-enzyme cascade reaction. The invention firstly provides a mutant of farnesyl pyrophosphate synthase, and the amino acid sequence of the mutant is shown as SEQ ID No.3. Compared with a wild type, the mutant is applied to enzyme catalytic synthesis of farnesol, so that the conversion rate of a gold product is remarkably improved. The invention also provides a three-enzyme cascade reaction preparation method of farnesol, which comprises the following steps: in the same reaction system, dimethyl allyl diphosphate is used as a substrate; according to the method, isopentenyl diphosphate isomerase, farnesyl pyrophosphate synthase or a mutant thereof and farnesyl pyrophosphatase are used as catalytic enzymes for enzymatic reaction to prepare all-trans-farnesyl alcohol. According to the invention, in a single reaction system, three key enzymes are combined for application, so that continuous catalysis, high-efficiency and high-selectivity preparation of all-trans-farnesol is realized, process steps are simplified, and a new way is provided for green and efficient preparation of farnesol.

The present invention relates to a class of isopentenyl flavonoid derivatives and their applications, and their structural formulas are shown in (I) and (2). The present invention has outstanding beneficial effects: 1. So far, there is no report in the prior art of a pharmaceutical composition with such isopentenyl flavonoid derivatives (1-2 shown in formula (I)) as an effective ingredient, and there is no report on the application of such isopentenyl flavonoid derivatives and their pharmaceutical compositions in the preparation of drugs for treating Zika virus (ZIKV) inhibitors. 2. The two isopentenyl flavonoid derivatives obtained by the present invention can significantly inhibit the expression of ZIKV E protein; and have no obvious toxicity to Vero cells, and their CC 50 All were greater than 100μM.

The present invention discloses use of a compound of the following formula I or a salt or deuterated product thereof in the preparation of an anti-glycation agent, wherein R1 is a hydroxyl group or hydrogen, R2 is a methoxy group or H, R3 is a hydroxyl group or hydrogen, R4 is a methoxy group, a hydroxyl group or H, R5 is an isopentenyl group, a hydroxyl group or hydrogen, R6 is a methoxy group or a hydroxyl group, and R7 is an isopentenyl group, a methoxy group, a hydroxyl group or hydrogen; wherein R5 and R7 are not both isopentenyl groups. This type of anti-glycation active molecule is a natural #imgabs0# ketone compound (with only one isopentenyl side chain), which is derived from synthetic or natural plants and has good safety. In the experiment of inhibiting the formation of AGEs, compared with the Yangshen drug aminoguanidine (AG) and 1,7-dihydroxy-2-methoxy-3-(3-methylbut-2-enyl) #imgabs1# ketone (1,7-dihydroxy-2-methoxy-3-(3-methylbut-2-enyl)xanthone), the anti-glycation effect is significant; the anti-glycation inhibition rate of the natural #imgabs2# ketone compound protected by the present invention (final concentration of 250 μM) is between 18%-53%, which is much higher than the anti-glycation inhibition rate (17%) of Yangshen AG at the same final concentration (250 μM). #imgabs3#

The present invention relates to a transformant having isoprene-producing ability The transformant of the present invention has superior isoprene-producing ability by introduction, into methanotrophic bacteria, a nucleotide sequence encoding exogenous isoprene synthase, a nucleotide sequence encoding exogenous isopentenyl diphosphate isomerase, and a nucleotide sequence encoding exogenous 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, and thus can be effectively used for isoprene biosynthesis.

The invention discloses a medical application of an isopentenyl flavonoid compound or an aryl naphthalene lignan compound in podophyllum hexandrum. The isopentenyl flavone compound and the aryl naphthalene lignan compound can obviously inhibit proliferation of FLS cells induced by TNF-alpha, and show anti-RA activity in a rat RA model induced by a complete Freund's adjuvant; the isopentenyl flavonoid compound can play an anti-RA effect by inhibiting JAK1, inhibit phosphorylation of proteins such as PLC-gamma, p38 and JNK in FLS cells induced by TNF-alpha, inhibit NF-kappa B intracellular expression, and inhibit a JAK1 downstream signal channel at the cellular level; the aryl naphthalene lignan compound can play an anti-RA effect by inhibiting TNF-alpha, inhibit expression of RIP-1 and TRAF2 / 4 / 5 in FLS cells induced by TNF-alpha, inhibit expression in NF-kB nucleus, and inhibit a TNF-alpha downstream signal channel at the cellular level.

The invention discloses an isopentenyl transferase and an application of the isopentenyl transferase in synthesis of bakuchiol. Specifically, recombinant saccharomyces cerevisiae for expressing the isopentenyl transferase is constructed, the isopentenyl transferase CcPT6 from fructus psoraleae is obtained through screening, the isopentenyl transferase CcPT6 can catalyze an isopentenylation reaction of a coumaric acid substrate to generate bakuchiol, and the activity of the isopentenyl transferase CcPT6 is higher than that of a reported sequence BAK36. The CcPT7 is obtained by truncating 54 amino acids at the N end of the CcPT6, the catalytic activity is improved by 74%, and the activity of the CcPT7 is higher than that of a reported BAK36 mutant. In addition, the invention also provides a recombinant saccharomyces cerevisiae and yarrowia lipolytica engineering strain for integrally expressing the isopentenyl transferase CcPT6 or CcPT7, so that the artificial biosynthesis of the bakuchiol is realized. The discovery of the isopentenyl transferase and the construction of the recombinant engineering strain have important meanings for promoting the biosynthesis of the bakuchiol.

The invention discloses an oxygen isopentenyl transferase participating in synthesis of sesquiterpene coumarin and an application of the oxygen isopentenyl transferase. Belongs to the technical field of gene engineering. The invention discloses an open reading frame sequence of a Xinjiang ferula oxygen isopentenyl transferase FsPT9 gene and an amino acid sequence coded by the Xinjiang ferula oxygen isopentenyl transferase FsPT9 gene. The FsPT9 gene is obtained by screening and cloning based on combined analysis of multi-omics data of ferula sinkiang, a yeast expression vector is constructed, and a catalytic mechanism of the FsPT9 is identified in vitro by taking 7-hydroxycoumarin and farnesyl pyrophosphate as substrates. A yeast heterologous expression system proves that the FsPT9 can catalyze 7-hydroxycoumarin and farnesyl pyrophosphoric acid to generate umbelliferone. The invention discloses a key enzyme for initial synthesis of sesquiterpene coumarin for the first time, and the key enzyme is an oxygen isopentenyl transferase which is reported for the first time and is used for catalyzing three isopentenyl units at the same time, so that theoretical guidance is provided for synthesis and transformation of pharmacodynamic substances of asafetida sesquiterpene coumarin.

The invention belongs to the technical field of medicines, and discloses application of C6-C3 derivatives in preparation of medicines for treating autoimmune demyelination diseases. The invention particularly relates to application of the isopentenyl-substituted C6-C3 derivative IB as shown in a formula (I) in preparation of drugs for preventing, relieving and / or treating autoimmune demyelination diseases. On a mouse experimental autoimmune encephalomyelitis model, the compound IB shows a good treatment effect, can effectively prevent and treat pathological changes and disease progression of diseases, relieve the severity of the diseases and relieve spinal cord inflammatory cell infiltration and spinal cord demyelination, and provides a new drug and a new structure for clinical treatment. # imgabs0 #

The application discloses a synthetic method of natural product Moracin N and belongs to the technical field of organic synthesis. The application takes an aldehyde containing a benzene ring as a starting raw material, introduces an isopentenyl group on the benzene ring through a Friedel-Crafts reaction, connects a hydroxyl protecting group to a No. 4 position hydroxyl group of the benzene ring to obtain an intermediate II, then uses a phosphorus ylide reagent to prepare the intermediate II into gem-dibromo olefin to obtain an intermediate III, then under the catalysis of a base and a copper catalyst, the gem-dibromo vinyl group is coupled with an ortho hydroxyl group to form a benzofuran ring to obtain an intermediate IV, then 3,5-dihydroxy bromobenzene is introduced to the right side of the benzofuran ring of the intermediate IV through a Suzuki-Miyaura reaction to obtain an intermediate VI, and finally the hydroxyl protecting group of the intermediate VI is removed to obtain Moracin N. The synthetic route of the application is simple, the reaction condition is mild, the safety is high, the required raw material is cheap and easy to obtain, the total cost is low, and the application is suitable for industrialized preparation.

The invention discloses a compound with T-type calcium channel inhibitory activity, a composition and application and a screening method thereof, and belongs to the technical field of medical chemistry and biological medicine. The compound takes a 6-isopentenyl isomer of parvacol as a parent nucleus, has excellent inhibitory activity and selectivity through structural optimization design and virtual screening, can improve DHT-induced hair follicle function inhibition or regulate scalp microenvironment calcium steady state, and has a good application prospect. The compound can be used for preparing Cav3 family T-type calcium channel inhibitors and medicines for preventing alopecia and / or promoting hair growth. The compound can be prepared into a solution, a gel, a cream or a liposome in vitro preparation. The compound disclosed by the invention not only has the effects of preventing alopecia, promoting hair growth and the like, but also reduces the risk of skin sensitization and reactivity, and improves the stability and process accessibility of an external preparation.

The present invention relates to a preparation method of 7,3(2’,4’)-trihydroxy-5-methoxy-6-isopentenyl coumarin. The preparation method includes: using 2,4,6-trihydroxybenzaldehyde as the starting material, through alkylation, protection group introduction, methylation reaction, and deprotection group reaction to generate 4,6-dihydroxy-2-methoxy-3-allylbenzaldehyde; then carrying out a condensation reaction with 2,4-dihydroxyphenylacetic acid in acetic anhydride solvent to obtain 7,3(2’,4’)-trioxo-substituted-5-methoxy-6-allyl coumarin; subsequently carrying out olefin metathesis with an olefin to obtain 7,3(2’,4’)-trioxo-substituted-5-methoxy-6-isopentenyl coumarin; and finally obtaining 7,3(2’,4’)-trihydroxy-5-methoxy-6-isopentenyl coumarin through deacetylation reaction.

This invention discloses a protein with O-isopentenyltransferase activity, related biomaterials, and applications, belonging to the field of biotechnology. This invention screened and cloned an O-isopentenyltransferase gene (GlOPT), which can be used to prepare O-isopentenyltransferase. Experiments have confirmed that this O-isopentenyltransferase can catalyze the in vitro conversion of 8-hydroxybergamot lactone to arugulatin; catalyze the conversion of bergamotol to isoimperatorin; and catalyze the conversion of xanthotoxin to imperatorin. This provides technical support for the production of isopentenyl furanocoumarin-like active ingredients from arugula, lays the foundation for research on the biosynthetic pathways of coumarin compounds, and has significant theoretical and practical implications.

This invention relates to isopentenyl flavonoids, their preparation methods, and applications, belonging to the field of natural product chemistry. Specifically, it involves the separation of five isopentenyl flavonoids—daphengiratriprenylone D, (3"'R)-daphnegiravone F, (3"'S)-daphnegiravone F, daphnegiravone E, and daphnegiralin H—from *Daphne giraldii* Nitsche, a plant in the Thymelaeaceae family, using silica gel column chromatography, macroporous resin chromatography, ODS column chromatography, and HPLC. Their inhibitory activity against HepG2 and Hep3B cells was tested. Ultimately, it was found that these compounds exert their antitumor effect by inhibiting the growth activity of tumor cells. The preparation method of this invention is simple, reproducible, and yields high-purity compounds with significant antitumor activity.

The invention discloses a method for extracting isopentenyl flavonoid compounds from black mulberries, which adopts an ultrasonic-assisted ionic liquid extraction method and comprises the following steps: crushing the black mulberries, mixing the crushed black mulberries with ionic liquid, soaking the mixed black mulberries, and carrying out ultrasonic extraction. Compared with a traditional water extraction method, the total content of the extracted isopentenyl flavonoid compounds sanggenone D and sanggenone C is increased by about 3.00 times. Meanwhile, the extraction method is green, efficient and energy-saving, and avoids environmental pollution to a greater extent.

The application relates to application of an isopentenyl transferase FnPT1 and belongs to the technical field of enzymology. The isopentenyl transferase FnPT1 screened and verified by the application is different from most reported flavonoid isopentenyl transferases, the isopentenyl transferase FnPT1 can catalyze isopentenyl modification reaction of flavonoid glycoside which is a relatively large substrate, the isopentenyl transferase FnPT1 has potential application in flavonoid glycoside synthetic biology, and has important application value.

The invention discloses a method for generating an isopentenyl polyphenol compound through biological catalysis, and belongs to the technical field of biological engineering. According to the present invention, mutants G326W, M8 and M7 are adopted as biocatalysts, kaempferol, quercetin, myricetin, naringenin and the like are adopted as receptor substrates, DMAPP / GPP is adopted as a donor substrate, and a variety of isopentenyl products with different chemical selectivity are catalyzed and synthesized; wherein when kaempferol is used as a receptor substrate, the yield of the mutant M8 is increased by about 45 times compared with that of a wild type, the chemical selectivity is also greatly improved, a C8-site trans-isopentenylation product is obtained, the mutant G326W is isopentenylated at a C3'site, and in addition, the catalytic geranylation activity of the mutant M7 is higher than 90%. Therefore, the mutant provided by the invention has application value in the aspect of synthesis of isopentenyl polyphenol compounds.

The invention relates to a preparation method of a natural product icaritin. The method comprises the following steps: performing MOM protection on a compound 1 to obtain an intermediate compound 2, reacting with p-methoxybenzaldehyde to obtain an intermediate compound 3, and reacting under alkaline catalysis to obtain a compound 4; removing MOM protection in an acidic environment to obtain 5, and reacting 5 with an isopentenyl derivative under the catalysis of Lewis acid to obtain icaritin 6; according to the method, synthesis steps are remarkably shortened, most of existing routes need about ten steps, and the route provided by the invention can realize synthesis of icaritin only through five-step reaction; through reasonable route design, introduction of isopentenyl is innovatively carried out in the last step, so that side reactions are reduced, and the purification difficulty is reduced.