2results about How to "Change conformation" patented technology

This invention provides a fully premixed PCR kit and its Taq DNA polymerase variant. The kit includes primers for PCR amplification, a PCR amplification buffer, and a Taq DNA polymerase mutant. The Taq DNA polymerase mutant is obtained by replacing the amino acid sequence of the 5'–3' exonuclease domain of wild-type Taq DNA polymerase with that of SEQ ID NO.2. The Taq DNA polymerase variant of this invention integrates the endonuclease 1 domain of *Thermococcus barbata*, endowing Tb-Taq with selective 5'→3' exonuclease activity. It has no cleavage activity at the 5' end of blunt-ended double-stranded DNA, while maintaining its hydrolytic ability to TaqMan probes during PCR amplification. This effectively solves the problem of probe cleavage and degradation during long-term storage of fully premixed kits, thereby avoiding phenomena such as increased fluorescence background, decreased fluorescence intensity of the amplification curve, Ct value lag, and significant decrease in detection sensitivity.

The present application relates to the technical field of biological medicine, in particular to an anti-tumor grifola frondosa water-soluble protein complex preparation and a preparation method thereof.The preparation raw materials of the complex preparation include the following components in parts by weight: grifola frondosa water-soluble protein 2-3 parts, fermentation preparation 0.8-1.2 parts, rosemary acid 0.1-0.2 parts.