3results about How to "Change conformation" patented technology

This invention provides a fully premixed PCR kit and its Taq DNA polymerase variant. The kit includes primers for PCR amplification, a PCR amplification buffer, and a Taq DNA polymerase mutant. The Taq DNA polymerase mutant is obtained by replacing the amino acid sequence of the 5'–3' exonuclease domain of wild-type Taq DNA polymerase with that of SEQ ID NO.2. The Taq DNA polymerase variant of this invention integrates the endonuclease 1 domain of *Thermococcus barbata*, endowing Tb-Taq with selective 5'→3' exonuclease activity. It has no cleavage activity at the 5' end of blunt-ended double-stranded DNA, while maintaining its hydrolytic ability to TaqMan probes during PCR amplification. This effectively solves the problem of probe cleavage and degradation during long-term storage of fully premixed kits, thereby avoiding phenomena such as increased fluorescence background, decreased fluorescence intensity of the amplification curve, Ct value lag, and significant decrease in detection sensitivity.

The present application relates to the technical field of biological medicine, in particular to an anti-tumor grifola frondosa water-soluble protein complex preparation and a preparation method thereof.The preparation raw materials of the complex preparation include the following components in parts by weight: grifola frondosa water-soluble protein 2-3 parts, fermentation preparation 0.8-1.2 parts, rosemary acid 0.1-0.2 parts.

The invention discloses a PVDF / Ca-POM composite fiber membrane, a preparation method thereof and application of the PVDF / Ca-POM composite fiber membrane in preparation of a wearable flexible piezoelectric sensor, and belongs to the technical field of functional materials. The composite fiber membrane is prepared from PVDF (Polyvinylidene Fluoride) and Ca-POM (Polyoxymethylene) through electrochemical spinning. PVDF has piezoelectricity, and Ca-POM has high flexibility and a high length-diameter ratio; double networks can be formed between the PVDF and the Ca-POM, and the existence of the Ca-POM restrains the movement of a PVDF chain, so that the mechanical property of the composite material is further improved; meanwhile, due to the high dipole moment effect between Ca-POM and PVDF, the conformation of a PVDF chain can be changed, the relative content of a beta phase can be increased, the piezoelectric property of the composite material is effectively improved, the sensitivity of the composite material serving as a wearable flexible piezoelectric sensor is guaranteed, and therefore the composite material has important application prospects in the field of wearable flexible piezoelectric sensors.