31results about How to "Change conformation" patented technology

The invention discloses a method for preparing a silk fibroin-alginate composite film, and belongs to the fields of composite materials and biotechnology. According to the method, brown algae extracted marine biomass sodium alginate serving as a substrate is mixed with waste silkworm cocoon extracted high-molecular-weight silk fibroin-alginate to prepare the dual-component composite film through tape casting by adopting supramolecular self-assembly and ionic crosslinking processes. The silk fibroin-alginate composite film has more intermolecular hydrogen-bond interactions, the comprehensive mechanical performance of the film can be improved, the moistening, water absorption and moisture absorption performance of the composite film can be remarkably improved due to the special interpenetrating mesh structure, and possibility is provided to loading of other sustained-release substances. The composite film is prepared from natural and green raw materials, and integrates excellent biocompatibility, non-toxicity, biodegradability and other properties of silk fibroin and sodium alginate, and can be applied to the industry of beautifying and skin-protecting masks. The preparation method is simple in preparation process, realizes solution recycling, is environmentally friendly, and has an excellent application prospect.

The invention discloses a preparation method of anti-bacterial silver-ion ceramic. The method comprises steps as follows: (1) ceramic clay serving as a ceramic preparation raw material is beat into slurry with a beater, the slurry is poured into a stirring machine to be stirred and mixed uniformly, then the slurry is injected into a ceramic mold, and a ceramic clay adobe is prepared; (2) an oxide containing silver is added to ceramic glaze and mixed uniformly, and silver-ion ceramic glaze containing an effective germicidal ingredient is prepared; (3) the ceramic glaze containing the silver ions is applied to the ceramic clay adobe, and a ceramic clay adobe coated with the silver-ion ceramic glaze containing the effective germicidal ingredient is prepared; (4) a photocatalyst with an anti-bacterial function is sprayed to the ceramic clay adobe coated with the silver-ion ceramic glaze containing the effective germicidal ingredient; (5) the ceramic clay adobe coated with the ceramic glaze and prepared in the step (4) is put into a kiln to be fired, and the anti-bacterial silver-ion ceramic is prepared. The ceramic prepared with the method has good anti-bacterial performance and can effectively inhibit bacterium breeding.

The invention relates to a viscosity reduction high efficiency water-reducing polycarboxylate superplasticizer and a preparation method thereof. The superplasticizer is prepared by intermittent dropwise adding of macromonomers like TPEG monomer, unsaturated hydroxy acid monomer or its derivative, or unsaturated acid with a trans-structure for free radical polymerization reaction under the action of a reductant, an oxidant and a chain transfer agent. The anchoring group and side chain in the superplasticizer are molecular structures in block distribution, and can give full play to electrostaticrepulsion and steric-hinerance effect. In addition, the hydroxyl, carboxyl and other polar groups introduced by the unsaturated hydroxy acid monomer or its derivative monomer increase the proportionof hydrophilic groups and reduce the surface tension of the cement pore solution. In addition, in terms of the trans-structural body represented by fumaric acid and dihydroxyfumaric acid derivatives,because of the special structure, i.e. the stiffness of the monomer itself and the distribution of two carboxyl groups on the opposite side of the side chain, a stretching structure easily exhibits inspace to reduce the possibility of molecular chain entanglement and significantly increase the adsorption effect of the polycarboxylate superplasticizer on the cement surface, thereby enhancing the viscosity reduction effect of the polycarboxylate superplasticizer.

The invention discloses a self-assembled collagen-stimulated microsphere with a naturally constituted shell-core structure and a preparation method thereof. The electrostatic interaction between polycationic polymers and sodium hyaluronate (HA) is used to change the conformation of HA molecule and reduce the viscosity of HA neutral solution. The formation of micron-sized HA microspheres in emulsion system is promoted. The precipitation of microspheres is promoted by the electrostatic interaction between polycationic natural polymers and HA and by the condensation crosslinking reaction of amines and acids. The method skillfully solves the problem of high viscosity of high molecular weight HA neutral solution, and has the advantages of simple procedure and environmental protection. The prepared collagen-stimulated microspheres have a shell. The self-assembled structure of the nucleus is composed of natural macromolecules, which can be rapidly degraded by specific biological enzymes whenside reactions occur, thus ensuring the safety of injection products. At the same time, the microspheres have good mechanical strength and long degradation time, can effectively stimulate the secretion of collagen for a long time, and have good application prospects in the field of soft tissue filling, especially in the field of medical cosmetic filling.

The invention relates to the technical field of flexible electrodes, and relates to a full-flexible transparent film electrode as well as a preparation method and application thereof. The full-flexible transparent film electrode and is formed by blending a water-soluble polymer serving as a dopant and a conductive polymer. The preparation method comprises the following steps of: dissolving a water-soluble polymer in a polar organic solvent, heating and stirring the water-soluble polymer and the polar organic solvent, uniformly stirring and mixing an obtained mixed solution with a PEDOT: PSS solution to obtain a full-flexible transparent film solution, dispensing the full-flexible transparent film solution on a substrate, and preparing a full-flexible transparent film on the substrate through spin coating, blade coating or screen printing; and annealing and drying the full-flexible transparent film and the substrate, then transferring the full-flexible transparent film and the substrate into water, separating the full-flexible transparent film from the substrate through ultrasonic treatment, thereby obtaining the full-flexible transparent film electrode. According to the full-flexible transparent film electrode provided by the invention, the prepared full-flexible transparent film electrode has good conductivity and transparency, also has good mechanical properties, and can bear a certain degree of strain.

The invention discloses a urine sample RNA stabilizing solution and a preparation method thereof. The urine sample RNA stabilizing solution is prepared from 0.01 to 2M of dithiothreitol, 2M to 6M of guanidinium isothiocyanate, 1 to 200 mM of ethylenediamine tetraacetic acid, 0.05 to 0.5 mM of diethyl pyrocarbonate and ultrapure water. The preparation method comprises the following steps: adding acertain amount of the chemical reagent into ultrapure water, adjusting the pH value to 4-4.2 with hydrochloric acid, fixing the volume, and carrying out sealed storage. The urine sample RNA stabilizing solution provided by the invention can effectively maintain the stability of RNA in a urine sample, can realize preservation and transportation of large-volume urine, and is suitable for subsequentRNA extraction and fluorescent RT-PCR.

The invention discloses a preparation method for a rice milk beverage with pleasant flavor. The preparation method comprises the following steps that rice is pretreated and then placed into a water bath vibrator, alkaline protease is added for enzymolysis, inactivation is conduced, and the system pH value is adjusted to be neutral; micronization treatment is conducted, dried yeast is added for fermentation, sterilization and spray drying are conducted, and then a first material is obtained; water is added into the first material, the system pH value is adjusted to be 6.4-6.7, gelatinization treatment is conducted, and then a second material is obtained; and maltose, sodium alginate and sodium carboxymethylcellulose are added into the second material and then are stirred to be uniform, olive oil is added to conduct emulsification treatment, then a mixture is sent to be subjected to homogenization treatment, sterilization is conducted, and then the rice milk beverage with the pleasant flavor is obtained. According to the preparation method, the obtained rice milk beverage is smooth and rich in tasting, rich in fragrance, extremely rich in nutrient substance and low in cost.

The invention discloses a method for preparing a rice protein functional drink by using rice dregs, and belongs to the technical field of rice deep processing. In the present invention, the rice dregs are soaked in warm water and then subjected to ultrasonic pretreatment, followed by two high-pressure homogeneous treatments under the conditions of 30-55 MPa instantaneously to obtain the upper light phase material and the lower layer heavy phase starch liquid, and the upper layer light phase material to maintain Keep the pressure at 90-100MPa for 20 minutes, concentrate to obtain heavy-phase protein slurry; concentrate and dry the lower-layer heavy-phase starch liquid to obtain starch; add purified water to the protein slurry to adjust the pH value; add starch, heat up to 80-85°C, and gelatinize at 0.5 Hours; add white sugar, thickener, stabilizer, stir well; add vegetable oil containing spaghetti, and emulsify in the mixing pot for 10 minutes; add flavoring agent, stir evenly, keep warm at 75-85°C, and sterilize and pack. The invention improves the extraction rate and purity of the rice protein and enhances the extracted protein quantity, and the rice protein is made into a cereal beverage, which is nutritious and healthy, and has low cost.

The invention discloses a preparation method of high tenacity silk fibroin gel. Ordinary silk fibroin solution undergoes crystallization-inducing treatment to obtain crystalline silk fibroin solution, then mixes it with ordinary silk fibroin solution, and finally obtains high-tenacity silk fibroin gel under the action of self-assembly. This technology can control the gelation time, toughness and other properties of hydrogel by changing the concentration of silk fibroin solution, the size and content of crystalline silk fibroin, self-assembly temperature and other parameters. The preparation process of the present invention is mild, no toxic organic reagents such as cross-linking agents are needed, the preparation conditions are green and controllable, and there is no waste residue, which provides a new method for the preparation of functional hydrogels and can be used in biomedicine and tissue engineering and other fields.

The invention discloses a self-assembled collagen stimulating microsphere with a natural shell-core structure and a preparation method thereof. Utilize the electrostatic interaction between polyamino polycationic natural polymer and sodium hyaluronate (HA) to change the conformation of HA molecules, reduce the viscosity of high molecular weight HA neutral solution, and promote the formation of micron-sized HA microspheres in the emulsification system . Utilize the electrostatic interaction between polyamino polycationic natural polymer and HA and the condensation and cross-linking reaction of acid amine to promote the precipitation of microspheres. The method skillfully solves the problem of high viscosity of high molecular weight HA neutral solution, and has simple steps and environmentally friendly technical route. The prepared collagen-stimulated microspheres have a shell-core self-assembly structure, and the components are all natural polymers, which can be rapidly degraded by specific biological enzymes when side reactions occur, ensuring the safety of product injections; at the same time, the microspheres have good Excellent mechanical strength and long degradation time can effectively stimulate collagen secretion for a long time, and have a good application prospect in the field of soft tissue filling, especially medical cosmetic filling.

A viscosity-reducing high-efficiency water-reducing polycarboxylate water-reducer and its preparation method, the water-reducer is composed of macromonomers such as isopentenol polyoxyethylene ether monomers, unsaturated hydroxy acid monomers or derivatives thereof, Or the unsaturated acid with trans structure is prepared by free radical polymerization under the action of reducing agent, oxidizing agent and chain transfer agent by intermittent dropping. The anchor groups and side chains in the water reducer of the present invention are block-distributed molecular structures, which can give full play to the electrostatic repulsion and steric hindrance effects; in addition, the hydroxyl groups introduced by unsaturated hydroxyacid monomers or their derivative monomers Polar groups such as carboxyl and carboxyl groups increase the proportion of hydrophilic groups and reduce the surface tension of the cement pore solution; in addition, the trans structure represented by fumaric acid and dihydroxy fumaric acid derivatives, due to its special The structure, that is, the rigidity of the monomer itself and its two carboxyl groups are distributed on the opposite side of the side chain, which makes it easy to show a stretched structure in space, reduces the possibility of molecular chain entanglement, and significantly increases the polycarboxylate superplasticizer. Adsorption on the surface of cement, thereby improving the viscosity-reducing effect of polycarboxylate superplasticizer.

The invention discloses a multiple-effect polyclone antibody for animals, prepared according to the following method with steps of: A, adopting grouped poly immunogen polypeptide as immunogen, which at least contains the sequence of the immunogen polypeptides as shown in SEQ ID NO:1, SEQ ID NO:2 and SEQ ID NO:3, or a sequence with immunogenicity derived from the sequences of SEQ ID NO:1, SEQ ID NO:2 and SEQ ID NO:3 by substituting, deleting, and / or adding one or more amino acid; B, immunizing animals: immunizing a target animal with the immunogen polypeptide of the step A and obtaining antiserum; and C, obtaining polyclone antibody: separating and purifying the antiserum immunoglobulin obtained in the step B to obtain the polyclone antibody. The polyclone antibody disclosed by the invention has extensive application in preparing integrate disease control drugs and immunopotentiating drugs for livestock.

The invention relates to an absorbable orthopedic apparatus material having antibiosis and bone growth promotion functions, and a preparation method thereof. The orthopedic apparatus material is obtained by compounding medical pure magnesium or magnesium alloy with an absorbable high-molecular polymer, the surface of the pure magnesium or magnesium alloy has a porous ceramic layer, the surface of the porous ceramic layer is a functional load layer loaded with bone morphogenetic proteins and copper ions with B-type gelatin as a carrier by adopting an electrophoresis-electrodeposition process, the volume fraction of the pure magnesium or magnesium alloy is 1-99%, the micropore aperture and the thickness of the porous ceramic layer on the surface of the pure magnesium or magnesium alloy are 1-100[mu]m and 1-100[mu]m respectively, and the surface of the porous ceramic layer is the functional load layer loaded with bone morphogenetic proteins and copper ions with B-type gelatin as the carrier by adopting the electrophoresis-electrodeposition process. An orthopedic apparatus can slowly release copper ions having a sterilizing effect and bone morphogenetic proteins having a bone growth promotion effect in the use process, can be applied to restore and fix various bone injuries, and has a wide application prospect.

The invention discloses a preparation method of silk fibroin thixotropic gel. Firstly, common silk fibroin solution and crystallized silk fibroin particle solution are prepared, then the crystallized silk fibroin particle solution is mixed with common silk fibroin solution of specific concentration, and left standing to obtain silk fibroin thixotropic gel. This technology can control the gelation time and light transmittance of hydrogel by changing the concentration of silk fibroin solution, the concentration of crystallized silk fibroin particle solution, the mixing ratio of the two, and the treatment temperature. The preparation process of the present invention is mild, no toxic organic reagents such as cross-linking agents are needed, the preparation conditions are green and controllable, it is convenient for large-scale preparation, there is no waste residue, green and pollution-free, and it is easy for industrialization.

The invention discloses a multiple-effect polyclone antibody for animals, prepared according to the following method with steps of: A, adopting grouped poly immunogen polypeptide as immunogen, which at least contains the sequence of the immunogen polypeptides as shown in SEQ ID NO:1, SEQ ID NO:2 and SEQ ID NO:3, or a sequence with immunogenicity derived from the sequences of SEQ ID NO:1, SEQ ID NO:2 and SEQ ID NO:3 by substituting, deleting, and / or adding one or more amino acid; B, immunizing animals: immunizing a target animal with the immunogen polypeptide of the step A and obtaining antiserum; and C, obtaining polyclone antibody: separating and purifying the antiserum immunoglobulin obtained in the step B to obtain the polyclone antibody. The polyclone antibody disclosed by the invention has extensive application in preparing integrate disease control drugs and immunopotentiating drugs for livestock.

The invention discloses a method for realizing efficient co-production of malt syrup and rice protein by adopting rice processing by-products. Broken rice and rice bran are adopted as the raw materials. The method comprises the following processing steps: (1) carrying out mechanical pretreatment; (2) carrying out ultrasonic treatment; (3) carrying out mild biological activating; (4) carrying out degreasing pretreatment; (5) carrying out hydrolysis and liquefying by adopting alpha-amylase; (6) carrying out separating, namely, carrying out centrifugalizing on the liquefied liquid obtained in the step (3) at 3200r / min, thus obtaining the supernate and sediment; (7) preparing broken rice and rice brain protein; (8) carrying out modified treatment on saccharifying enzyme; and (9) preparing the broken rice and rice bran malt syrup. With the adoption of the method provided by the invention, the malt syrup and rice protein by-products with good quality can be prepared, and the utilization value of the rice processing by-products is favorably improved.

The invention belongs to the technical field of agricultural product processing and storage, and provides an invention that improves the foamability of egg white liquid and prolongs its shelf life. Prepare fresh egg white liquid, add compound protease in a certain proportion, and enzymatically hydrolyze after proper ultrasonic treatment. After the enzymatic hydrolysis is completed, the egg white liquid is deenzyme-killed and sterilized by ultrasound, and finally pasteurized properly by raising the temperature again. The foaming power of the obtained egg white liquid is increased by 3 times, the foam stability has no significant difference, and the storage period is extended by 4 times. This process effectively solves the problems of foamability and shelf life, meets the needs of enterprises, has high utilization rate of equipment, and is conducive to improving the benefits of egg white liquid enterprises.

The invention discloses a method for efficiently co-producing maltodextrin and rice protein through rice processing byproducts. The method is characterized in that broken rice and rice bran are used as the raw materials. The method comprises the following processing steps: (1) mechanically crushing; (2) ultrasonically processing; (3) lightly biologically activating; (4) debranching and preprocessing; (5) hydrolyzing and liquidating with alpha-amylase; (6) separating; (7) preparing broken rice and rice bran protein; (8) preparing broken rice and rice bran maltodextrin. With the adoption of the method, high-quality broken rice and rice bran maltodextrin and rice protein byproducts can be prepared, so that the utilization value of the rice processing byproducts can be further increased.

The invention discloses a blood collection tube with the function of protecting and stabilizing the precursor of gastrin releasing peptide in serum or plasma, comprising a test tube, a sealing rubber plug and a plastic outer cap are arranged at the opening of the test tube, and the test tube is prefilled with cheese Protectant formulated with protein, sodium lauryl sulfate and aprotinin. Because the protective agent is pre-filled in the blood collection tube, the storage time of the ProGRP sample is greatly extended (the sample can be stored stably for 7 days at 2-8°C). On the one hand, it is convenient for clinical operation and application. Retesting can be carried out, which reduces the probability of false negative test results and improves the accuracy and clinical application value of the test results of this item.

The invention provides a production method of photo-induced energy-storage fluorescent drawings. Photo-induced energy-storage noctilucent powder, natural pigment and an adhesive are used as raw materials to produce a base material, and spun silk, paper and cloth are used as substrates for drawing, wherein a method for preparing the base material comprises the following steps that 1, an anionic surfactant is dissolved in n-butyl alcohol to form an oil phase solution; 2, cow glue is added into water, and through stirring, a collagen solution is obtained; 3, the photo-induced energy-storage noctilucent powder and the natural pigment are added into the collagen solution in the step 2, and through stirring, a pigment stock solution is obtained; 4, the pigment stock solution obtained in the step3 is added into the oil phase solution, and through ultrasonic concussion, a base material stock solution is obtained; 5, the base material stock solution obtained in the step 4 is subjected to spraydrying to obtain the base material. According to the production method of the photo-induced energy-storage fluorescent drawings, the anionic surfactant is used for preparing the oil phase solution, the prepared pigment stock solution is added into the oil phase solution, through ultrasonic concussion, oil phase molecules are broken, and then the pigment stock solution is evenly dispersed in the oil phase molecules to improve the water resistance of the pigment stock solution.

The invention provides an artificial ligament which comprises an inner core and an outer layer from inside to outside in sequence. The inner core is made of ozonized polyethylene glycol terephthalate, and the outer layer is made of polysaccharide or polymer polymerized from hydrophilic group contained allyl monomer and is grafted on a free radical locus of the inner core. A preparation method of the artificial ligament includes: subjecting a ligament of a polyethylene glycol terephthalate material to reaction with ozone so as to obtain the polyethylene glycol terephthalate with a free radical; under protection of inert gas, subjecting a solution containing monomer molecules and the polyethylene glycol terephthalate containing the free radical to sealed polymerization reaction so as to obtain the artificial ligament. The artificial ligament is good in biocompatibility, and capable of restraining bacterial infection, changing protein conformation adhered on surface of a material, reducing inflammation and promoting adhering, growing and fibroblast of fibroblast after being planted in a human body. Collagen protein synthesis on the surface of the artificial ligament is improved, and infiltration of new ligament tissue is facilitated.