The invention relates to a moisture-preserving, freshness-retaining and color-retaining fresh flower specimen making method. The moisture-preserving, freshness-retaining and color-retaining fresh flower specimen making method includes the following steps that S1, newly-picked whole fresh flowers are completely soaked into first stationary liquid, stationary color retaining is conducted for 5 h to 7 h, the obtained flowers are taken out and washed with flowing water for 8 min to 12 min, after washing is completed, residual washing water on surfaces are blown to be removed, and the obtained flowers are ready for use; S2, the fresh flowers ready for use in S1 are completely soaked into second stationary liquid, stationary color retaining is conducted for 3 h to 5 h, the obtained flowers are taken out and washed with flowing water for 4 min to 6 min, after washing is completed, residual washing water on the surfaces are blown to removed again, and the obtained flowers are ready for use; the fresh flowers ready for use in S2 are soaked with preserving liquid or embedded with high molecular resin, and specimens are obtained. The moisture-preserving, freshness-retaining and color-retaining fresh flower specimen making method has the advantages that the obtained fresh flower specimens have the characteristics of moisture preserving, freshness retaining and color retaining, it is avoided that fresh flowers are dried with air and dried beside or over a fire, and the made fresh flower specimens have soft tissue and are not rotted, subjected to color changing and deformed; the fidelity of the made fresh flower specimens is higher, the color and the shape of the specimens can be kept for a long time, and the technology is initiative at home and abroad.