74results about How to "Improve scientific research efficiency" patented technology

The invention discloses a transparent soil model test device for mining area pile foundation bearing force. The transparent soil model test device comprises a base, a sand box, a transparent soil model box, an electronic scale, a pile body, a loading device, a CCD industrial camera and a laser linear generator. The sand box is arranged on the base. An open hole is formed in the bottom of the sand box. The electronic scale is arranged below the open hole and used for measuring the weight of outflow sand. The transparent soil model box is embedded in the upper portion of the sand box. The bottom face of the transparent soil box is a layer of emulsion film and used for isolating transparent soil from sand soil. The pile body is arranged in the transparent soil. A pile cap is arranged at the pile top. The load is applied to the pile cap. The pile body is provided with marking points for monitoring displacement of the pile body. The laser linear generator is arranged on one side of the test device. By the adoption of the transparent soil model test device, mining subsidence caused by coal mining is simulated through a sand leakage method, so that the mining area earth surface soil mass subsidence process is simulated; and meanwhile, the method can be used for earth surface soil mass horizontal movement or vertical movement caused by similar human engineering activities by changing the sand leakage position and the pore size.

The invention discloses a visual saturated dynamic water-bearing sand bed permeation grouting test device and method. The visual saturated dynamic water-bearing sand bed permeation grouting test device comprises a visual model box, a grouting pump, a sprinkling plate, an oil pump, a constant head water tank, a laser sheet source transmitter, an industrial camera and a data collection system, wherein slurry is injected into the visual model box by the grouting pump through a slurry delivery pipe; openings are uniformly formed in a bottom plate; a layer of filter net covers the bottom plate; an oil receiving tank is arranged at the bottom of the visual model box and connected with the oil pump through an oil delivery pipe; the oil pump is connected with the constant head water tank; a locating water tank is also connected with the sprinkling plate connected to the top of the visual model box through the oil delivery pipe; the laser sheet source transmitter is arranged at one side of the visual model box; laser light emitted from the laser sheet source transmitter penetrates through transparent soil through the visual model box to form a uniform speckle field; the shooting direction of the industrial camera is located in a normal direction of a laser plane; and the camera is connected with the data collection system and records the change of the speckle field in real time.

The invention relates to a testing device for simulating a submarine landslide and a testing method thereof. The invention aims to provide the testing device for simulating the submarine landslide which is simple in structure and convenient in operation and the testing method thereof. Through the technical scheme, the testing device for simulating the submarine landslide is characterized in that the testing device is provided with a model casing; the model casing is provided with a glass face convenient to observe the internal situation of the model casing; the model casing is internally provided with a slantly arranged slide rail; the lower end of the slide rail is rotatably connected with a supporting plate; the supporting plate is fixed to the model casing; the upper end of the slide rail is connected to the model casing through a slide rail inclination adjusting mechanism; the upper surface of the slide rail is covered with a cushion layer; a mudflow generating mechanism for releasing mud is arranged above the upper part of the slide rail; and a monitoring mechanism for monitoring the state of the mud on the slide rail is arranged corresponding to the slide rail. The testing device is applicable for the field of ocean engineering research.

The invention relates to a method for preparing a rare earth ion co-doped optical fiber preform. The method comprises the following steps: respectively preparing an aqueous solution of a codoping agent of a plurality of rare earth ions and aluminum ions; selecting a quartz base tube and connecting into an MCVD lathe, and sequentially depositing an isolating layer and a loose core layer after high-temperature polishing; fixing a hollow preform body containing the loose core layer on a lifting device, and allowing a preform body part to be soaked in the solution; changing the co-doping ratios and concentrations of various ions in the solution according to the co-doping ratio distribution and concentration distribution of ions of a pre-designed preform core in the axial direction, and simultaneously adjusting the soaked length of the preform body through the lifting device; allowing the soaked preform body to be re-connected into the MCVD lathe, and vitrifying the loose core layer after drying and dehydrating, and finally, collapsing the preform body into a solid preform. The method can be used for flexibly and precisely controlling the distribution of co-doping ratios and doping concentrations of ions of the preform core part in the axial direction, and a very efficient and simple method is provided for finding out the best ion co-doping ratio and doping concentration for different types of rare earth ion co-doped optical fibers.

The invention discloses a mining area subsidence singe pile static load model test device and a test method. The device comprises a model slot of which the front of peripheral surface is transparent at least, wherein the slot bottom serves as a bearing plate; a sand leakage hole is formed in the slot bottom; a blocking orifice plate is arranged below the slot bottom and is used for blocking the sand leakage hole; the blocking orifice plate is supported by using a supporting column; a sand layer, a polyethylene film and a clay layer are sequentially paved in the model slot from the bottom upwards; a model pile is embedded in the clay layer; soil pressure boxes are embedded at the bottom and in the periphery of the model pile; a strain gage is arranged on the pile body; a loading device is arranged on the model top; the soil pressure boxes and the strain gage are connected to a data acquisition unit outside the model slot; and dial indicators are respectively arranged on the tops of the model pile and the clay layer. According to the test method disclosed by the invention, surface soil movement deformation rules in multiple mining areas can be accurately simulated, and the mining area surface deformation process under multiple geological conditions and different curvature change processes can be simulated.

The invention discloses an identification and measurement method for a high-temperature evaporation-type spray schlieren image. Aiming at a morphological structure that the high-temperature evaporation image is comparatively complicated, a partition processing strategy is adopted; the identification and measurement method comprises the following steps: firstly, detecting a spray liquid phase region and a mixing region by adopting an improved background subtraction edge method; then, detecting a spray steam region and a mixing region by an inter-frame operation edge method; finally, performing post-processing steps of fusion, morphology and the like on the identified regions to obtain the complete outline of a spray evaporation region; subsequently, performing spray characteristic measurement calculation to obtain the quantitative indicator of a spray region. The identification and measurement method disclosed by the invention is capable of identifying and automatically measuring the high-temperature evaporation-type spray image of a premixed combustion-type constant volume bomb, and has the advantages that a threshold value is automatically determined, the processing speed is high, and the accuracy is high, and the like.

The invention discloses an experimental method used for research of thin film water migration laws. The experimental method comprises following steps: experimental soil sample is delivered into a soil column in layers; ionized water with a certain concentration is delivered into a water supply device, and a small amount of plant oil is added for oil seal of the ionized water; a certain amount of water is added into the water supply device every a period of time so as to ensure that the water level is higher than a buffer layer of the soil column; in experimental processes, data acquired by a humidity temperature sensor is sent to a data acceptance and analysis system via a wireless data sent terminal for analysis; and a certain period of time later, the soil is sampled for another time so as to measure water content and ion concentration of soil of different depth. The experimental method is capable of realizing accurate research on thin film water migration rate under different soil conditions, so that reliable experimental base is provided for research of water migration of soil in arid areas. The invention also discloses an experimental device used for the experimental method which is used for research of thin film water migration.

The invention discloses a device for researching pile-soil interaction under horizontal load through transparent soil and a test method of the device. The device comprises a supporting framework and amodel slot placing plate, wherein two slide rails are arranged on a slide rail fixing plate fixed on the supporting framework; the lower surface of the model slot placing plate is fixedly equipped with slide wheels which are matched with the slide rails; a model slot and a limiting device are arranged on the upper surface of the model slot placing plate; a model pile fixing plate is connected onto the supporting framework above the model slot; a mounting hole is formed in the model pile fixing plate; a vibration wire type sensor is mounted on the side surface, approximately perpendicular to the slide rails, of the model pile; a lifting jack for pushing the model slot to slide along the slide rails is mounted on the slide rail fixing plate; a laser light source system is placed on the opposite side of the lifting jack; an image acquisition device for acquiring an image in the model slot is arranged at the perpendicular side of the laser light source system; and the image acquisition device, a helium-neon laser unit, the lifting jack and the vibration wire type sensor are connected with a controller.

The invention belongs to the field of artificial intelligence, and particularly relates to a mouse embryo organ recognition and scoring method and a system. The method comprises the following steps: collecting original images of mouse embryos in different periods, and carrying out the manual marking; inputting the mouse embryo original image and the annotation file into a Mask-RCNN network for training to obtain an organ recognition model; intercepting organs in the marked image from the original image, taking the organs and corresponding scores as training set data, and training different convolutional neural networks to obtain an image scoring model capable of scoring development of each organ; inputting a to-be-recognized mouse embryo original image into the organ recognition model, and outputting all organs in the image; intercepting all organs from the original image, and respectively inputting the organs into an image scoring model to obtain a development score of each organ; and obtaining a total score. According to the method, the organs in the mouse embryo can be quickly and accurately recognized, and the current development stage of each organ can be judged.

The invention discloses a shield tunnel segment mould capable of manufacturing multiform tenons. The shield tunnel segment mould comprises a standard block mould, a first adjacent block mould, a second adjacent block mould and a capping block mould. The standard block mould, the first adjacent block mould, the second adjacent block mould and the capping block mould separately comprise base plates,end head plates correspondingly connected to the two ends of the base plates in a matched mode, lateral plates and top plates, and mould cavities used for pouring segments are enclosed by the base plates, the end head plates, the lateral plates and the top plates. The base plates are provided with longitudinal joint steel moulds and circumferential joint steel moulds, the lateral plates are connected with the end portions, far away from the base plates, of the longitudinal joint steel moulds, and the end head plates are connected with the end portions, far away from the base plates, of the circumferential joint steel moulds. Tenon changing blocks are arranged on the lateral plates, and location holes are formed in the end head plates. The shield tunnel segment mould capable of manufacturing the multiform tenons can produce different forms of tenons and different forms of circumferential joints and longitudinal joints, and through arbitrary combinations of the different forms of tenonsand the different forms of circumferential joints and the longitudinal joints, so that a plurality of different forms of segments are formed. The shield tunnel segment mould capable of manufacturingthe multiform tenons has high operability.

The invention relates to a method for dealing with literature information. The method includes the steps of obtaining the kernel keywords of the literature, and carrying out similar aggregation on the obtained kernel keywords; constructing the logical structure relation between the kernel keywords in one piece of literature, and forming a logic interconnection system of all the kernel keywords in different literature pieces; establishing indexes between the kernel keywords and correlation network node information and the corresponding literature pieces; according to attention kernel keywords input by users, querying and displaying direct correlation information and indirect correlation information corresponding to the kernel keywords. According to the technical scheme, the use efficiency of the literature can be improved.

The invention discloses a grit matrix cleaning device and relates to the technical field of sand culture. The grit matrix cleaning device comprises a bracket, a driving device and a filter box. The bracket is movably connected to a stirring container, a stirring shaft is arranged in the stirring container, stirring blades are arranged on the stirring shaft, the top of the stirring container is opened, and a movable screen is arranged on the top opening of the stirring container; the driving device is arranged on the bracket, and the output end of the driving device is connected to one end of the stirring shaft; the filter box comprises a first box body and a second box body, the first box body can be placed in the second box body, and a water outlet hole is formed in the side wall of the first box body. The invention also discloses a grit matrix cleaning method of the grit matrix cleaning device. According to the grit matrix cleaning device and the grit matrix cleaning method, large grit can be removed and soluble nutrients attached to the surface are cleaned, so that a cleaned grit soil matrix meets demands on scientific research tests such as nutrient stress and grade size uniformity of a culture matrix, and therefore, the scientific research efficiency is increased greatly.

The invention discloses an anchor plate pull-out test device, system and method based on transparent soil, belongs to the technical field of anchor plates, and aims to not only reduce disturbance to asoil body when an anchor rod is mounted in a model test, but also achieve an effect of predicting a three-dimensional deformation field of the soil body by observing any tangent plane in a three-dimensional deformation process of the soil body, and there is a condition of observing the displacement deformation of the soil body between the plates in the drawing process of the horizontal double anchors arranged at the same height. The device comprises a soil filling model box, a soil baffle, an anchor rod, an anchor plate and a sealing plug, wherein one side face of the soil filling model box is an open side, the soil baffle is installed on the open side of the soil filling model box in a sliding mode and the anchor rod penetrates through the soil baffle to enter the soil filling model box.The anchor plate is installed in the soil filling model box in the opening side, the anchor rod penetrates through the anchor plate, the soil baffle is provided with a plurality of open holes, and the open holes can be sealed through sealing plugs; and two side surfaces of the filling model box are transparent.

The invention belongs to the technical field of determination of gaseous hydrocarbon hydrogen isotope composition in a mineral inclusion, and particularly discloses a device and a method for extracting gaseous hydrocarbon hydrogen isotope composition in a mineral inclusion. According to each path of helium, a water trap filled with magnesium perchlorate is used for absorbing and drying trace waterpossibly existing in the helium, so that the influence of the trace water in carrier gas on a gaseous hydrocarbon hydrogen isotope composition test result is avoided. On-line continuous flow mode isadopted, and a mineral inclusion bursting device, a methane oxidation and product water collection device and a stable isotope mass spectrometer are connected; online heating burst of minerals in a helium flow environment, online oxidation of methane, online collection of oxidation product water and electric heating release of water enter a stable isotope mass spectrometer to be tested, and compared with a traditional offline method, the sample amount is greatly reduced, so that the beneficiation workload and the scientific research cost are effectively reduced, and the scientific research efficiency is improved.

An experimental probe for measuring physical rock parameters of water saturated mudstone under a high-pressure drainage condition adopts the technical scheme as follows: a hole is formed in the center of the upper end face of a probe base; a groove is formed in the bottom surface of the probe base and corresponds to the hole in position, the diameter of the groove is larger than that of the hole, and the groove is communicated with the hole; one end of a high-pressure resistant drainage rod is inserted and fixed in the hole, a supporting pad is mounted in the groove and is clung to the end inserted in the hole, of the high-pressure resistance drainage rod; a filter net is arranged under the supporting pad; a filter net threaded cover plate is arranged on the bottom surface of the groove, the supporting pad and the filter net are connected through screw extrusion of the filter net threaded cover plate; holes are formed in centers of the supporting pad and the filter net threaded cover plate, the diameters of the holes are equal to the inner diameter of the high-pressure resistant drainage rod, and the supporting pad, the filter net threaded cover plate and the inner diameter of the high-pressure resistant drainage rod are positioned on one line.

The invention discloses a digestive juice for cartilage tissues. The digestive juice comprises the following components: 1-3 mg / ml of type II collagenase; 1-3 mg / ml of type IV collagenase; 5 ml of neutral protease; 12 units / ml of DNA enzyme; 10% of FBS (V / V); and 0.053 mg / L of CaCl2. The beneficial effects are as follows: a cell acquisition rate is high, cells have relatively high activity, a clustering rate is low, the damage to the cells is relatively small, the transcriptome change is low, and the subsequent culture or single-cell transcriptome sequencing can be performed.

The present invention discloses a multifunctional cloning vector, wherein the construction method comprises adopting a resistance gene expression self-saving manner and directly adopting the conventional antibiotic to screen positive clones, and specifically comprises: designing a pair of primer sequences with 5' terminal phosphorylation, adopting PUC19 plasmid as a template, and adopting high-fidelity PCR amplification to obtain the multifunctional cloning vector. With the vector prepared by using the method, various PCR products can be efficiently cloned, efficient cloning efficiency of the blunt-ended PCR product and the general Taq enzyme amplification product can be achieved, and the vector of the present invention can be adopted to completely replace the blunt-ended cloning vector and the TA cloning T vector. The method for preparing the multifunctional cloning vector has advantages of general positive rate of greater than 90%, low false positive rate, low test cost, less experiment operation steps, convenience and rapidness. The invention further discloses the use method of the multifunctional cloning vector.

The invention provides an iconography scientific research project scanning intelligent reservation system and a use method, and the system comprises a scientific research project submitting module which is used for enabling a scientific research applicant to submit a scientific research project needing to be scanned in a preset time period, and automatically calculating the score of the scientific research project based on a preset calculation rule; a project statistics module which sequentially accumulates scores from the scientific research projects with high scores to the scientific research projects with low scores based on experimental subjects of the scientific research projects, wherein when the accumulated scores reach a preset threshold value, the scientific research projects corresponding to the accumulated scores are counted and defined as selected projects; and a scanning reservation module which is used for calculating the number of the experiment objects capable of being reserved in each selected item in a reservation period, and releasing the reservation time of the experiment objects capable of being reserved in the selected item in the reservation period at specified time according to a preset reservation rule. The scientific research efficiency and the machine room utilization rate can be improved, and the arrangement of scientific research projects can be flexibly and reasonably adjusted.

The invention relates to an infrared low-light-level dual-channel image fusion distortion correction target plate, Black target, white target, A temperature control device and a fixing bracket, the shape of the target plate is black-and-white grid alternating, The black target can be heated and the white target cannot be heated. Temperature measuring elements are installed on both the black and white targets. The temperature difference between the black and white targets is controlled by temperature control device, so that the black and white contrast images presented by infrared detectors canbe obtained. At that same time, the contrast image of black and white change can also be present due to the different colors of the black and white target when the low-light-level detector observe. After infrared and low-light-level dual-channel image fusion, the distortion correction of infrared low-light-level dual-channel image fusion can be realized by analyzing the target mismatch and correcting it.

The invention discloses a mutual calling method based on MATLAB and ABAQUS. The method is based on an operation mechanism of MATLAB and ABAQUS. The advantages of MATLAB and ABAQUS are utilized, MATLABis used as a main program, the structural boundary dimension, material parameters, iteration conditions and the like are set in the main program, a data result of ABAQUS analysis is automatically called through the MATLAB main program, and automation of optimization design operation iteration is achieved. According to the method, the advantages of MATLAB in the aspects of programming, operation,data processing and graphic processing can be organically fused with the authority of ABAQUS in the strong finite element analysis aspect, the operation efficiency can be effectively improved, the automation of optimization design operation iteration is achieved, and a new design approach is provided for structure optimization design.

The invention relates to the technical field of simulation systems of new energy grid-connected power generation, in particular to a photovoltaic grid-connected power generation semi-real object simulation system; the photovoltaic grid-connected power generation semi-physical simulation system comprises a simulation program integration execution platform, a simulation machine, a real object part,a signal acquisition system of the real object part and a simulation photovoltaic power supply, wherein the simulation program integration execution platform is used for executing a program and transmitting a signal to the simulation machine and the signal is converted into an electrical signal for running the real object part through the simulation machine; the simulation photovoltaic power supply is used for providing a grid-connected required energy source for the real object part; and the signal acquisition system is used for collecting the electrical signals of the real object part and feeding the electrical signals to the simulation program integration execution platform. According to the system, the cost of a new energy power generation photovoltaic array and an electricity storagesystem thereof can be reduced, and the performance of a new energy grid-connected system can be integrally mastered. According to the system, the problem of instability caused by inaccurate tracking of the maximum power point of the new energy power generation is fundamentally solved, and the phenomenon of energy waste caused by oversaturation of the photovoltaic array under the actual working state is avoided.

The invention discloses a control structure for automatically changing samples in a closed high-pressure reaction chamber and an automatic sample changing method. The control structure comprises a worktable lifting mechanism, a bearing plate rotating mechanism and a bearing plate positioning mechanism, wherein the worktable lifting mechanism comprises a lifting rod; a supporting seat is arranged at the top of the lifting rod; the lifting rod is connected with a driving motor; the bearing plate rotating mechanism comprises bearing plates distributed in multiple layers, a rotating shaft framework and a rotating shaft control motor; each bearing plate is arranged on the rotating shaft framework; the bearing plates can rotate around the rotating shaft framework and the rotating shaft; the rotating shaft framework is fixed together with the rotating shaft and is connected with the control motor through the rotating shaft; a plurality of round holes and one opening are distributed in each bearing plate along the external circumference; each round hole is equipped with a bearing platform for placing a sample; positioning holes corresponding to the plurality of round holes are formed along the internal circumference of each bearing plate; the bearing plate positioning mechanism comprises a first positioning rod fixed at the top of a pressure chamber and a second positioning rod fixed at the bottom of the pressure chamber. According to the control structure and the automatic sample changing method disclosed by the invention, on the premise of not interrupting and not influencing a water-rock action in the pressure chamber, rock samples are automatically changed for a single-shaft or rheological compression test.

The invention discloses a method for evaluating the suitable degassing time of an XLPE flat plate sample or a cable. The method comprises the following steps: (1) preparing / selecting a sample; (2) performing sample pretreatment; (3) degassing treatment; (4) constructing an evaluation model; and (5) evaluating the degassing time. According to the method, the weight change rule of the samples is obtained through the degassing weight loss ratios of the multiple samples of the same specification, the weight loss change rate parameters are calculated with the average weight loss ratio as the reference, then the suitable degassing time is obtained, the method can accurately evaluate the suitable degassing time of various different XLPE formula materials, and the test method is simple, convenientto operate and high in universality. According to the method, the time for researchers to carry out heat treatment on the XLPE sample can be greatly shortened, the scientific research efficiency is improved, the degassing time cost of a cable manufacturer can also be reduced, and a scientific and effective analysis means is provided for XLPE material analysis and cable production in the aspect ofavoiding the influence of cross-linking by-products.

The invention discloses an unmanned ship formation deep towing operation system and method, and belongs to the field of ocean exploration equipment, the unmanned ship formation deep towing operation system comprises an overwater scientific investigation ship, an unmanned ship formation and an underwater deep towing system, and the unmanned ship formation is provided with a winch, a GNSS positioning system, an underwater sound positioning system, wireless communication equipment and a deep towing system. A scientific investigation ship carries a plurality of unmanned ship formations equipped with deep towing systems to jointly arrive at a measurement area, and the scientific investigation ship issues tasks and navigation information through an inter-ship communication system; the unmanned ship formation performs operation according to the scientific investigation ship task instruction; and the unmanned ship formation transmits the measured data to a scientific investigation ship laboratory in real time for data calculation and analysis. The problems that when a scientific investigation ship executes a submarine topography exploration task, the scientific investigation ship is single in execution task and low in working efficiency are solved, and rapid deep towing system operation of an unmanned ship formation on an inner sea area within the water depth of 4000 m is achieved.

The invention relates to the field of geotechnical engineering, and particularly provides an experimental device and method for simulating a multi-anchor coupling effect. The experimental device for simulating the multi-anchor coupling effect comprises a transparent model box, wherein a plurality of anchor rods which are parallel on the same vertical plane and are obliquely arranged relative to the edge of the model box are arranged in the transparent model box; The experimental device further comprises an arc-shaped perforated strip and an external loading device, wherein a plurality of openholes are formed in the arc-shaped perforated strip in the arc direction, the anchor rod body penetrates through the open holes through a steel strand to be connected with the external loading device,and the external loading device controls the steel strand to contract. The experimental device also comprises a transmission light source and a camera. The problem that in the prior art, the influence of deformation and displacement of an anchor rod on surrounding soil is simplified into plane strain, and the real influence of the anchor rod on the surrounding soil cannot be accurately reflectedis solved.

The invention discloses a bituminous mixture performance testing method and system based on triaxial numerical experimentation. The bituminous mixture performance testing method comprises the steps that experimental parameters are obtained; the experimental parameters comprise aggregate density, a mineral aggregate gradation, bituminous mixture density and a bituminous mixture porosity factor; simulated bituminous mixtures are generated; an initial bituminous mixture triaxial numerical experiment model is generated according to the experimental parameters and the bituminous mixtures; a mechanical model between the bituminous mixtures in the initial bituminous mixture triaxial numerical experiment model is built, and a bituminous mixture triaxial numerical experiment model is generated; andthe triaxial numerical experimentation is carried out for the bituminous mixture triaxial numerical experiment model, the shearing strength of the bituminous mixtures is calculated, and the testing of the bituminous mixture performance is finished. According to the bituminous mixture performance testing method and system based on the triaxial numerical experimentation, the testing of the bituminous mixture performance can be finished rapidly and accurately.

The invention discloses an elevating apparatus for an underwater detector. The elevating apparatus comprises an elevating rod (9) capable of moving relative to a fixed rack (1), a linear driving device used for driving the elevating rod (9) to move, a sealing device (7) used for dynamically sealing the elevating rod (9), a valve used for making the elevating rod (9) pass through and enter water, and a control device (7) used for controlling the linear driving device; and the sealing device (7) and the valve are sequentially connected from top to bottom, the linear driving device is connected with the elevating rod (9), and the elevating rod (9) and the sealing device (7) form dynamic seal. The apparatus fills the industry gap, and realize the rapid transfer between an underwater medium and an overwater medium, so the scientific research efficiency is increased.

The invention discloses construction of a cargo plasmid in an anabaena 7120 triparental transformation system and application method of the cargo plasmid. The plasmid pPT27 is formed by recombining nucleic acid elements in one step through a sequence and linkage independent cloning (SLIC) method. After a target nucleic acid sequence is connected, the cargo plasmid is generated, and the cargo plasmid and helping and combination plasmids are used for transferring an exogenous DNA (Deoxyribonucleic Acid) sequence into a host cell; a target mutant strain is screened through a marker gene. The invention further discloses the application method of the pPT27 plasmid, and green fluorescent protein (GFP) and fused protein (FdnifK) are transferred in. The cargo plasmid (pPT27) constructed by the invention can be used for functional analysis and protein expression of a target DNA segment; the cargo plasmid can be rapidly and efficiently constructed in a time-saving manner by applying the constructed plasmid and limitation on a DNA sequence can be avoided; the cargo plasmid has very important meanings in the aspect of researches of gene functions.