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External rapid detecting method for protamine usage amount and protamine gradient ACT cup

A rapid detection technology for protamine, which is applied in the field of determination of protamine usage, can solve the problems of increased bleeding, endangering the life of patients, and long blood clotting time, etc., and achieves the effect of simple operation and rapid detection

Inactive Publication Date: 2008-06-11
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the above-mentioned operation of neutralizing heparin, the method for determining the amount of protamine used is not scientifically sound, which often results in too little or too much protamine, so that repeated use of protamine
Too little or too much protamine used to neutralize heparin can cause blood clotting time to be too long, resulting in increased bleeding in patients. Repeated use of protamine can cause anaphylactic shock to patients, and sometimes even endanger the lives of patients

Method used

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  • External rapid detecting method for protamine usage amount and protamine gradient ACT cup

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The protamine gradient ACT cup in this embodiment is as figure 1 As shown, it includes a cup body 1, a mixing rod 2, a fixed frame 3 and a cover plate 5; the cup wall of the cup body 1 is provided with a blood injection scale line 4, and the cup body 1 is filled with kaolin or diatomaceous earth and milt. Protein 6; cover plate 5 is installed in the cup body, and is located on kaolin or diatomaceous earth and protamine 6, below the blood injection scale line 4; the lower end of the mixing rod 2 passes through the cover plate 5 and is inserted into the cup The lower part of the body and the upper end are used to connect with the ACT instrument; the fixing frame 3 is installed on the upper part of the cup body 1 and is fixedly connected with the cup body.

Embodiment 2

[0026] The concrete steps of assay method described in the present embodiment are as follows:

[0027] (1) Prepare protamine gradient ACT cup

[0028] The protamine gradient ACT cup used in this example is to add protamine to the existing ACT cup, and prepare ten protamine gradient ACT cups, and the cup numbers of each protamine gradient ACT cup are A, B, C, D, E, F, G, H, I, J. The dose of self-clay in each protamine gradient ACT cup is equal and the same as that of kaolin in the existing ACT cup. The amount of protamine is gradually increased by the gradient of 2.5 μg, and the protamine is a water preparation. The dose of protamine contained in each protamine gradient ACT cup is shown in Table 1:

[0029] Table 1 The dose of protamine contained in each protamine gradient ACT cup (unit μg)

[0030] cup number

A

B

C

D

E

F

G

H

I

J

Protamine Dosage in a Cup

2.5

5

7.5

10

1...

Embodiment 3

[0039] In order to verify the accuracy of the assay method of the present invention, the assay method of the present invention was used experimentally on three postoperative patients who needed to neutralize heparin.

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Abstract

A fast mensuration in vitro on the usage amount of the nucleoprotamine is provided, whose steps are that: first, prepare the nucleoprotamine grads ACT cup, which is a testing container filled with porcellanite or diatomite and the nucleoprotamine. At least two such cups are needed in one test; second, inject the whole blood of the patient into the nucleoprotamine grads ACT cup, whose amount is in the limit of meeting the requirement of the nucleoprotamine grads ACT cup; third, test the ACT value by the ACT instrument; fourth, calculate the dosage of the nucleoprotamine according to the nucleoprotamine amount in the nucleoprotamine grads ACT cup corresponding to the appropriate ACT value, and the dosage of the nucleoprotamine of the patient is equal to the a / b is multiplied by blood volume per kilogram of body weight and multiplied by the patient's weight, wherein, the a is the nucleoprotamine dosage in the nucleoprotamine grads ACT cup corresponding to the appropriate ACT value, while b is the whole blood amount of the patient injected into the nucleoprotamine grads ACT cup corresponding to the appropriate ACT value.

Description

technical field [0001] The invention belongs to a method for measuring the amount of protamine used, in particular to a method for measuring the amount of protamine used to neutralize heparin after surgery. Background technique [0002] Before cardiovascular, liver and kidney transplantation and other operations, due to the needs of the operation, a certain dose of heparin must be administered to the patient. After surgery, heparin must be neutralized with protamine. At present, neutralizing heparin is usually based on the heparin dose used by the patient before surgery, neutralizing the heparin with an equal dose of protamine, and then according to the activated whole blood coagulation time (ACT value for short) measured before the patient's heparinization, in the subsequent description Directly use "ACT value" to represent "activated whole blood coagulation time") and the activated whole blood coagulation time (ACT value) or coagulation status measured after neutralizing ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/86G01N33/50
Inventor 李刚杜磊贾梦醒刘进
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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