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Method and kit for detecting mycobacterium tuberculosis and nontuberculous mycobacterium

一种结核分枝杆菌、分枝杆菌的技术，应用在基于微生物的方法、生物化学设备和方法、微生物的测定/检验等方向，能够解决无法充分支应经费、诊断限制等问题

Inactive Publication Date: 2009-10-21
ASIAGEN CORP
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AI Technical Summary

Problems solved by technology

Routine diagnosis of Mycobacterium tuberculosis with LC instruments is still limited due to the inability to fully support the funds required for instrument setup and maintenance
[0016] Korean patent application KR20030075315 discloses a method for simultaneous and rapid detection of Tubercle Bacillus (TB) and nontuberculous Mycobacteria (NTM) using multiple-nested PCR. Although this method successfully increases its Amplifies the specificity of DNA fragments, but requires more knowledge of the target sequence to achieve

Method used

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  • Method and kit for detecting mycobacterium tuberculosis and nontuberculous mycobacterium
  • Method and kit for detecting mycobacterium tuberculosis and nontuberculous mycobacterium
  • Method and kit for detecting mycobacterium tuberculosis and nontuberculous mycobacterium

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Embodiment Construction

[0070] However, the following descriptions are only preferred embodiments of the present invention, and are not used to limit the scope of the present invention. All features and spirits of the present invention should be included in the claims of the present invention.

[0071] Materials and methods

[0072] 1. Primers and probes:

[0073] (1) The primer pair of Tbc1 and TbcR5-TB bacteria.

[0074] (2) The primer pair of NTM-M5 and NTM-RM3-NTM bacteria.

[0075] (3) TB probe-probe of Mycobacterium tuberculosis.

[0076] (4) MAC probe-probe of Mycobacterium avium.

[0077] (5) MK probe-probe of Mycobacterium kansasii.

[0078] (6) RGM probe-probe of Rapid Growers Mycobacterium.

[0079] 2. Clinical samples:

[0080] (1) Mycobacterium tuberculosis (TB)

[0081] (2) Mycobacterium avium (MAC)

[0082] (3) Mycobacterium kansasii (MK)

[0083] (4) Rapid Growers Mycobacterium (RGM)

[0084] 3. Purification of DNA from clinical samples:

[0085] (1) Take 1ml of one-fold ph...

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Abstract

The present invention relates to a method and a kit for detecting Mycobacterium tuberculosis (MTB) and Nontuberculous mycobacterium (NTM). The invention relates to the method and the kit for detecting Mycobacterium tuberculosis (MTB) and Nontuberculous mycobacterium (NTM) through a nested polymerase chain reaction (nested PCR).

Description

technical field [0001] The invention relates to a method for distinguishing mycobacterium tuberculosis and non-tuberculosis mycobacteria by using nested polymerase chain reaction (nested PCR) and a detection kit. Background technique [0002] Mycobacterium tuberculosis, a member of the genus mycobacterium among Gram-positive bacteria, nontuberculous mycobacterium, or atypical mycobacterium; nontuberculous mycobacterium Mycobacterium other than tuberculosis is a general term for bacteria in the genus Mycobacterium except Mycobacterium tuberculosis. Among them, Mycobacterium aviumcomplex was widely isolated from the specimens of AIDS patients, and non-tuberculous mycobacteria were paid attention to. [0003] Tuberculosis is the main fatal infectious disease in the world. At present, one-third of the population suffers from tuberculosis, of which 20 million people are active tuberculosis, and more than 50 million people are infected by multi-drug resistant tuberculosis bacteri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11C12R1/32C12R1/325
CPCC12Q1/689C12Q2600/16
Inventor 周锦生
Owner ASIAGEN CORP
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