21 results about "Atypical mycobacterium" patented technology

Provided herein are method of treating a patient with nontuberculous mycobacteria by administering nitric oxide to the patient for at least 100 days to increase functional capacity and quality of life of the patient.

The present invention provides a primer set for differentiating Mycobacterium tuberculosis complex and non-tuberculous mycobacteria and its application. The primer set includes the nucleotide sequences shown in SEQ ID NO: 1-118. The present invention collects and analyzes the identification genes of Mycobacterium tuberculosis complex and non-tuberculous mycobacteria from the database, designs specific primers for the identification genes, combines multiplex PCR technology and targeted high-throughput sequencing technology, improves the detection sensitivity by ultra-multiplex PCR amplification and enrichment of the target, and improves the detection specificity based on the sequence reading of the gene sequencing platform, so as to achieve accurate and sensitive differentiation of mycobacteria. It has low detection cost, short detection cycle, high sensitivity, good specificity, and can effectively identify mixed infections, and has important application value in the detection of tuberculosis / NTM diseases.

A novel CHI3L1 inhibitor provided in the present invention can efficiently inhibit or treat disease progression of nontuberculous mycobacteria-infected lung diseases by inhibiting CHI3L1. In particular, it has been identified that K284-6111 can inhibit or treat disease progression of nontuberculous mycobacteria-infected lung diseases by inhibiting CHI3L1, and by newly synthesizing derivatives of K284-6111, novel compounds having a CHI3L1 inhibitory ability superior to that of K284-6111 have been identified. Therefore, the novel CHI3L1 inhibitors provided in the present invention can inhibit or treat disease progression of nontuberculous mycobacteria-infected lung diseases by inhibiting CHI3L1.

The application discloses a primer, a probe composition for nontuberculous mycobacteria typing and application thereof, and provides a PCR primer and a probe for rapid detection and typing of 13 common NTM in clinic based on genome big data for carrying out pan-genomics analysis, and the specific genes of the common NTM in clinic are screened by using a pan-genomics method, and then typing primers and probes are designed based on the genes, so that the problems of insufficient accuracy and specificity in NTM typing are solved; the various NTM specific sequences screened guarantee the accuracy and specificity; the high sensitivity of the PCR method and the specificity of the sequences guarantee that various interference substances in the clinical samples can be avoided, and the clinical samples can be directly analyzed; the PCR detection is low in cost and low in equipment requirement, and can be carried out in primary hospitals, thereby providing an efficient and economical solution for the precise diagnosis of nontuberculous mycobacteria, the control of disease prevalence and the large-scale disease screening.

The present disclosure provides bismuth-thiol (BT) compositions and methods for treating nontuberculous mycobacterium infections and associated conditions in a subject in need thereof.

The present invention relates to a composition for preventing, alleviating, or treating nontuberculous mycobacteria infection, the composition comprising a thiopeptide compound. A composition according to one aspect of the present invention can prevent, alleviate, or treat infections or infectious diseases caused by nontuberculous mycobacteria.

The chitinase 3-like 1 (CHI3L1) inhibitor, specifically kasugamycin, provided by the present invention, suppresses the expression or activation of chitinase 3-like 1 (CHI3L1), thereby effectively preventing or treating infections with acid-fast bacteria, particularly non-tuberculous mycobacteria and various diseases caused by infection with acid-fast bacteria, particularly nontuberculous mycobacteria. In addition, by measuring the expression level of chitinase 3-like 1 (CHI3L1) protein, when the expression level is higher than that of a control group, it is possible to effectively predict infection with acid-fast bacteria, particularly non-tuberculous mycobacteria, the onset of diseases caused by infection with acid-fast bacteria, particularly non-tuberculous mycobacteria, or the prognosis of diseases related to infection with acid fast bacteria, particularly nontuberculous mycobacteria.

Disclosed herein are compounds of Formula I and methods for using the same for treating or preventing an infection caused by a Mycobacterium in a subject in need thereof. The method comprises administering to the subject the compound of Formula I, or a pharmaceutically acceptable salt thereof, or a pharmaceutical composition comprising the compound of Formula I.

This invention relates to combinations for treating nontuberculous mycobacterial diseases. Specifically, it relates to combinations of bedaquiline, macrolides (e.g., clarithromycin), and optionally ethambutol for use in the treatment of diseases associated with nontuberculous mycobacteria (NTM).

Provided herein are methods for preventing or treating an infection caused by a Nontuberculous mycobacterium (NTM) species in a subject comprising administering to the subject an effective amount of an antibody or an antigen-binding fragment thereof that binds to a tip region of a DNABII peptide. Also provided in are methods for sensitizing a biofilm to an antibiotic agent, wherein the biofilm comprises a Nontuberculous mycobacterium (NTM) species, the method comprising contacting the biofilm with an antibody or an antigen-binding fragment thereof that binds to a tip region of a DNABII peptide.

The present invention relates to an immunogenic composition comprising a malonate semialdehyde decarboxylase (MSAD) protein or a polynucleotide encoding same, and a vaccine composition and a pharmaceutical composition for preventing or treating nontuberculous mycobacteria infection, which comprise same. The vaccine composition and the pharmaceutical composition, which comprise the MSAD protein or the polynucleotide encoding same, of the present invention, have excellent preventive and therapeutic efficacy against nontuberculous mycobacteria infection. In addition, the vaccine composition and the pharmaceutical composition, which comprise the MSAD protein or the polynucleotide encoding same, of the present invention, have a greater effect of preventing and treating nontuberculous mycobacteria infection when administered intranasally, and have a greater effect of preventing and treating nontuberculous mycobacteria infection when used in combination with an immune adjuvant.

The invention provides a specific primer probe combination for identifying nontuberculous mycobacteria and a detection method. In the specific primer probe combination, a first round of amplification substance combination comprises at least one pair of SEQ ID NO.1 and 2, SEQ ID NO.4 and 5, SEQ ID NO.7 and 8, SEQ ID NO.10 and 11, SEQ ID NO.13 and 14, and SEQ ID NO.16 and 17, and a second round of amplification substance combination comprises SEQ ID NO.19 and 20 and a probe corresponding to the first round of amplification substance combination. According to the invention, nontuberculous mycobacteria are detected by an asymmetric PCR probe melting curve method. The detection method disclosed by the invention has the characteristics of simplicity in operation, high sensitivity, good repeatability and high specificity.

The present invention relates to a biomarker for diagnosing nontuberculous mycobacteria-infected lung disease and determining a treatment initiation time. In addition, the present invention relates to a method for diagnosing nontuberculous mycobacteria-infected lung disease and determining a treatment initiation time by using a chitinase 3-like 1 (CHI3L1) protein marker in blood. The present invention provides chitinase 3-like 1 (CHI3L1) as a biomarker for diagnosing nontuberculous mycobacteria-infected lung disease and determining a treatment initiation time. The biomarker of the present invention is remarkably useful in diagnosing nontuberculous mycobacteria-infected lung disease and determining a treatment initiation time, and thus is expected to be widely used in the field of health and medicine.

Disclosed herein are compounds of formula I and methods of using same to treat or prevent infections caused by mycobacteria in a subject in need thereof, comprising administering to the subject a compound of formula I, or a pharmaceutically acceptable salt thereof, or a pharmaceutical composition comprising a compound of formula I. JPEG2025536245000185.jpg61150

The present invention relates to a novel febuxostat derivative compound and to a composition for preventing or treating a nontuberculous mycobacterial infection, comprising same as an active ingredient. The compound of the present invention efficiently kills nontuberculous mycobacteria, including Mycobacterium shinjukuense and Mycobacterium kansasii, which exhibit low responsiveness to conventional standard therapies and a high frequency of resistance mutation occurrence, via host-directed therapy mediated by host macrophages, and thus can be effectively used as a fundamental therapeutic composition for various complications caused by nontuberculous mycobacteria infections.

According to the invention, systematic MIC determination is carried out on fast-growing and slow-growing nontuberculous mycobacteria, and it is found that TBA-7371 has stable low MIC in nontuberculous mycobacteria of different pedigree, so that it is technically ensured that TBA-7371 can reach an effective inhibitory concentration in vivo more easily and has broad-spectrum applicability. The invention further explores the combined medication effect of the TBA-7371 and a plurality of first-line anti-mycobacterium tuberculosis drugs, and finds that when the TBA-7371 is combined with other anti-tuberculosis drugs, the required concentrations of the other anti-tuberculosis drugs can be reduced.

The present invention aims to provide a method for rapidly, simply, and reliably identifying patients with nontuberculous mycobacterial lung disease (pulmonary NTM disease) compared to lung cancer patients, healthy controls, or chronic airway inflammatory diseases other than pulmonary NTM disease, as well as a marker to be used in this method. [Solution] According to the present invention, it is possible to provide a biomarker consisting of the SLC26A4 gene, the SLC26A4 protein, or the SLC26A4-AS1 gene for distinguishing between subjects with pulmonary NTM disease and lung cancer patients or healthy controls or chronic airway inflammatory diseases other than pulmonary NTM disease, a method of identification using the gene or protein, and a kit containing the gene.

This invention discloses the use of a pyridine derivative. Specifically, it discloses the use of substance X in the preparation of drugs for inhibiting nontuberculous mycobacteria, drugs for treating and / or preventing nontuberculous mycobacterial infections, nontuberculous mycobacterial inhibitors, or nontuberculous mycobacterial antibacterial agents, wherein substance X is a compound represented by Formula I or a pharmaceutically acceptable salt thereof. The pyridine derivative of this invention exhibits good inhibitory activity against nontuberculous mycobacteria, and has low cytotoxicity and good safety.

The invention provides a real-time fluorescent PCR (polymerase chain reaction) detection method aiming at nontuberculous mycobacterium detection and identification, and relates to the technical field of biological detection, the method comprises the following operation steps: S1, extracting DNA (deoxyribonucleic acid) of a to-be-detected sample; S2, carrying out first PCR amplification: designing primers: designing primers Mp1 and Mp2 by using a 16S rRNA gene 3'end and a 23S rRNA gene 5 'end of mycobacterium; taking the extracted DNA of the sample to be detected as a template, and carrying out primary PCR amplification by using primers Mp1 and Mp2; s3, carrying out real-time fluorescent PCR detection and identification on the nontuberculous mycobacterium species by taking a first PCR amplification product as a real-time fluorescent PCR template; a plurality of groups of specific detection primers and probes are designed and are used for detecting and identifying the nontuberculous mycobacteria; and carrying out real-time fluorescent PCR detection and second detection and identification by using the specific detection primer and the probe. Real-time fluorescent PCR detection and identification are carried out by using the specific detection primer and the probe, the detection sensitivity is improved while the specificity of the nontuberculous mycobacterium species is maintained, and the kit is suitable for blood specimen detection and is also suitable for other clinical specimens such as sputum specimens, culture isolates and the like.

To provide a novel compound with antibacterial activity against drug-resistant nontuberculous mycobacteria, and a method for producing the same.SOLUTION: The invention provides a compound represented by the formula in the figure, or a salt thereof. (In the formula, one of X1 and X2 represents a group represented by -A-B-D, while the other represents -OH; A is a sugar chain derived from a pentose, hexose, or a polymer of one of them; B is an optionally substituted divalent hydrocarbon group; and D is -N3 or an optionally substituted triazolyl group.)SELECTED DRAWING: Figure 3

To provide a combination in treatment of nontuberculous mycobacterial diseases.SOLUTION: The present invention relates to a combination of bedaquiline, a macrolide (e.g. clarithromycin) and, optionally, ethambutol for use in the treatment of a disease associated with nontuberculous mycobacteria (NTM).SELECTED DRAWING: None