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Method for processing microscope video image

A technology of video image and processing method, applied in the field of microscopic video image processing, to achieve the effect of uniform brightness

Inactive Publication Date: 2012-05-02
NINGBO UNIV
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Problems solved by technology

[0004] The technical problem to be solved by the patent of the present invention is to provide a microscopic video image processing method based on the above-mentioned prior art, which can compensate the uneven brightness and exposure problems of the optical microscope image formed by various light sources or underexposure of the camera, The exposure time of the microscopic video image can be dynamically adjusted during the video image acquisition process

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  • Method for processing microscope video image

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Embodiment Construction

[0029] The present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.

[0030] Such as figure 1 The exposure processing method of the shown digital microscopic image, it comprises the following steps:

[0031] Step 1. Acquisition of video images: the image acquisition device of the microscope collects a frame of video images;

[0032] Step 2, judging the exposure of the video image: judge whether the exposure of the video image collected above is normal according to the gray histogram of the collected video image: the abscissa of the gray histogram represents the gray level from 0 to 255, and the vertical coordinate Indicates the number of pixels, through which the image grayscale distribution information can be obtained simply and intuitively, and can directly reflect the exposure of the image. Since the grayscale histogram is the statistics of the frequency of image pixels in different grayscales, the exposure ...

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Abstract

The invention relates to a method for processing a microscope video image, in particular to an exposure processing method of a digital microscope image, which is characterized in that: for the characteristics that the illumination of an optical microscope digital image system is not uniform, an exposure situation is determined according to a brightness histogram so as to adjust the exposure time; and a brightness gain curve is drawn according to a pure background slice-free background image to obtain a brightness gain equation, and a brightness adjusting factor when the brightness of the pure background slice-free background image is adjusted to be uniform can be calculated through the equation. Under the same condition, a brightness value of a pixel point corresponding to a sliced image is adjusted through the brightness adjusting factor to obtain the brightness homogeneity of the image. Due to the adoption of the method, the homogeneity adjustment of the video image of the optical microscope digital image system can be precisely realized.

Description

technical field [0001] The invention relates to a method for processing microscopic video images. Background technique [0002] Microscopic images are easily affected by illumination and optical distortion of the system, resulting in uneven brightness of the image, which affects the analysis of microscopic images. For example, in biomedical images, the length of nerve fibers and the size of various cell particles need to be measured; in cell diagnosis, especially when cell identification is required, qualitative and quantitative results need to be obtained. When completing these tasks, due to the uneven brightness of the image, the processing accuracy is restricted. This requires adjusting the exposure of the image sensor according to different lighting environments during microscopic imaging, so that the exposure of the entire image is moderate and the brightness is uniform. [0003] The existing processing methods can be roughly divided into two categories: one is the tr...

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Application Information

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IPC IPC(8): H04N5/235H04N5/243
Inventor 王新坡杨鸣邱元芳蒋金涛邵华章湖王建宏过森君
Owner NINGBO UNIV
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