Culture medium for producing spores, and optimizing process and application thereof

Abstract

The invention provides a culture medium for producing spores, and the optimizing process and application thereof, which are particularly applied to marine bacillus which hardly generates spores in a common liquid medium. The culture medium comprises the following components in percentage by weight: 0.5 to 3 percent of cornmeal, 0.1 to 1 percent of bran, 0.1 to 0.5 percent of soybean cake meal, 0.01 to 0.1 percent of potassium dihydrogen phosphate dodecahydrate, 0.05 to 0.3 percent of calcium carbonate anhydrous and the balance of water. The culture medium has the advantages of readily available raw materials, low cost and simple preparation, and can obviously improve the spore formation rate and total active spores of marine bacillus 9912 and marine bacillus subtilis 3728. By the culture medium and the application of the culture medium, essential conditions are provided for developing bacillus licheniformis 9912 active spore preparations and other related active spore preparations.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and more specifically relates to a culture medium for high-yield active spores of marine bacillus optimized by response surface method and its application, especially a kind of medium for producing high active spores of marine bacillus licheniformis which is not easy to produce spores culture medium and its application. Background technique [0002] Fungal diseases have brought huge losses to agriculture and forestry. At present, the control of crop diseases mainly depends on the use of broad-spectrum chemical pesticides. However, the extensive use of chemical pesticides has led to increasing resistance of pests and diseases, high pesticide residues and serious pollution in agricultural and livestock products, food and water sources have been seriously polluted, and human health and the ecological environment have been seriously threatened. Therefore, it is very necessary and urgent to grad...

AI Technical Summary

Problems solved by technology

However, marine Bacillus licheniformis 9912 is not easy to produce spores under conventional culture conditions, which seriously hinders its development into an active spore preparation that is highly efficient, non-toxic, low-cost, easy to store and transport, and has a long shelf life. The limiting factors of spore culture, and further optimize its fermentation conditions to increase the rate of spore production and the total number of spores

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