Preparation method and application of monascus mother starter
A technology of Monascus mother and Aspergillus mother, which is applied in the field of red yeast rice fermentation to achieve the effects of increasing the number of spores, improving equipment utilization, and improving quality
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[0033] A preparation method of Monascus mother koji, comprising the following steps:
[0034] Step S1, strain activation
[0035] Take 50 μL of the Monascus spore aqueous solution stored at 4°C and spread it on a fresh medium, cultivate at a temperature of 30°C, and cultivate for 6-8 days to obtain activated spores;
[0036] Step S2, seed solution cultivation
[0037] Wash the activated spores in step S1 with sterile water, and filter through 6 layers of gauze to make a concentration of 1×10 4 -10 6 The spore suspension per mL is inoculated into the liquid medium according to the inoculation amount of 8% to 10% (v / v), the culture temperature is 28-30° C., and the culture time is 2-3 days to obtain an activated seed liquid;
[0038] Step S3, the preparation of song kind
[0039] Put brown rice flour and coarse wheat bran into a container (specifically, a triangular flask), and mix the brown rice flour and coarse wheat bran at a mass ratio of 9:1 to form a koji medium, and a...
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