Method for culturing dental pulp cells and method for transporting extracted tooth for preservation

A technology of dental pulp cells and isolated teeth, which is applied in the field of transport of isolated teeth, and can solve problems such as the pain of the collection object

Inactive Publication Date: 2014-01-08
GENE TECH SCI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, when stem cells are collected from the above-mentioned tissues, the recipient often has to bear risks such as pain. For example, bone marrow needs to be harvested by bone marrow aspiration, and the harvest from fat tissue needs to be accompanied by liposuction.
In addition, although the collection of umbilical cord blood is almost painless, it requires equipment that meets strict collection conditions, and the timing of collection is limited to the time of delivery

Method used

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  • Method for culturing dental pulp cells and method for transporting extracted tooth for preservation
  • Method for culturing dental pulp cells and method for transporting extracted tooth for preservation
  • Method for culturing dental pulp cells and method for transporting extracted tooth for preservation

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Embodiment

[0064] Examples of the present invention are shown below, but the present invention is not limited to these examples.

[0065] First, an example of the transfer (preservation) of isolated teeth (primary teeth, permanent teeth) according to the method of the present invention will be shown.

[0066]

[0067] deciduous teeth

[0068] According to the dental method, the shaking deciduous teeth without caries and forming more than 2 / 3 of the roots of the successor permanent teeth, or the non-shaking deciduous teeth with caries that have not reached the enamel and only dentine and formed more than 2 / 3 of the roots of the successor permanent teeth. After unplugging, put it into a sterile test tube filled with α-MEM medium, refrigerate at low temperature (4°C), and store for 24 hours including the delivery time.

[0069] permanent teeth

[0070] Extraction of impacted teeth, supernumerary teeth or convenient extraction teeth without caries, untreated pulp according to the dental ...

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Abstract

Provided are a method for culturing dental pulp cells without compromising the in vivo function of the dental pulp cells, and a method for transporting an extracted tooth for preservation. The method for obtaining dental pulp cells includes: a step for forming a linear groove in the surface of an extracted tooth; a step for dividing the extracted tooth along the groove and exposing the dental pulp; and a step for soaking the extracted tooth in a culture medium and conveying the soaked tooth in a state maintained at a temperature appropriate for cell storage.

Description

[0001] related application [0002] This application claims the priority of Japanese Patent Application No. 2009-289090 filed in Japan on December 21, 2009, and the contents thereof are incorporated into this application. technical field [0003] The present invention relates to a method for transferring an isolated tooth for culturing and preserving dental pulp cells, and particularly relates to improving the preservation of the cells in transferring an isolated tooth having a dental pulp containing dental pulp cells. Background technique [0004] Since ancient times, research and development of regenerative medicine has been active in regenerating tissues or organs lost due to trauma, disease, accident, etc., using collected cells, as is known by blood transfusion. In recent years, the specific process of regenerative medicine has targeted the following process: collecting undifferentiated stem cells, culturing them in vitro, differentiating them depending on the situation...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/00C12N5/0775A61L27/00A61L27/38
CPCC12N5/0654A01N1/02A61L27/00C12N5/00C12N5/0602
Inventor 斋藤一郎大久保亮大友宏一
Owner GENE TECH SCI CO LTD
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