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Podophyllum hexandrum tissue culture seedling and exercising seedling transplanting acclimatization method

A technique for tissue culture seedlings of Peach chinensis, applied in horticultural methods, botanical equipment and methods, cultivation, etc., can solve the problems of slow research progress, no systematic research on seedling hardening and transplanting and domestication technology, and achieve The effect of expanding the population, facilitating management after transplanting, and improving the survival rate

Active Publication Date: 2013-09-04
GANSU ACAD OF SCI INST OF BIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The research on the tissue culture technology of Taoerqi began in the 1980s, and the research progress was slow, and there was no systematic research on the technology of hardening seedlings and transplanting domestication.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Taoer seven tissue cultured seedlings hardened seedlings

[0032] 1) Seedling hardening in the bottle: Select the seedlings of Taoerqi tissue culture with more than 2 true leaves, stems, and roots, remove the sealing film of the tissue culture bottle, and continue to cultivate in the cultivation room for 5 days. 26℃, 17~21℃ at night, photoperiod: 10 h / d, light intensity: 1000~1500Lux, humidity: 70~90%.

[0033] 2) Transplanting and hardening seedlings outside the bottle: Take out the seedlings of Taoerqi from the bottle, wash the root and rhizome culture medium with warm water, and then transplant them into the transplanting medium that is sterilized by dry heat at 160°C for 2~3 hours. The culture conditions are the same as that of hardening seedlings with openings in the bottle. The transplanting substrate is a mixture of peat soil and perlite at a ratio of 2:1; after transplanting, pour 1500 times of carbendazim solution and place it in the cultivation ro...

Embodiment 2

[0034] Example 2 Transplanting and Acclimatization of Tissue Cultured Seedlings of Taoerqi

[0035] 3) Greenhouse transplanting and post-transplanting management: transplant the Taoerqi seedlings transplanted into the matrix in the cultivation room of Example 1, and move them to the greenhouse in October to November. The conditions in the greenhouse are: the average minimum temperature is 10°C, the average maximum temperature is 36°C, the average minimum humidity is 70%, and the average maximum humidity is 98%. After transplanting, the seedlings are watered twice a week, and after 20 days, they will pass through the slow seedling stage, and the new leaves will gradually unfold, thicken and become dark green, and the transplanting survival rate is 98.1%. In the coldest month of January, after the aboveground parts wither, water once every two weeks. After the new buds emerge from March to April, they will be transplanted step by step. The transplanting substrate is peat soil....

Embodiment 3

[0037] Example 3 Transplanting and Acclimatization of Tissue Cultured Seedlings of Taoerqi

[0038] 3) Greenhouse transplanting and post-transplanting management: transplant the Taoerqi seedlings in the substrate in the cultivation room of Example 1, and move them to the greenhouse in March to April. The temperature and humidity conditions in the greenhouse are: the average minimum The temperature is 10°C, the average maximum temperature is 27°C, the average minimum humidity is 60%, and the average maximum humidity is 98%. After transplanting, the seedlings were watered 2 times a week. The transplanting survival rate was 95.0%. After new buds and new roots grow out, expand the transplanting step by step, and the transplanting substrate is peat soil;

[0039] 4) Field domestication: Transplant the surviving seedlings in the above-mentioned greenhouses, move them out of the greenhouse from May to June and place them under natural shade and ventilation conditions of woody tre...

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Abstract

The invention discloses a podophyllum hexandrum tissue culture seedling and exercising seedling transplanting acclimatization method. The podophyllum hexandrum tissue culture seedling and exercising seedling transplanting acclimatization method comprises the steps of bottle-opening seedling exercising of podophyllum hexandrum tissue culture seedlings and young seedlings in a cultivation chamber, transplanting matrix seedling exercising, greenhouse transplanting, management after transplanting, field acclimatization and the like. The method can improve transplanting survival rate of the podophyllum hexandrum tissue culture seedlings by above 15%, podophyllum hexandrum plants survived after field acclimatization grow robustly, and main leaves can reach 4-5. Compared with field artificial planting plants with the same growth age limit, the main leaves are 2-3 more than those of the field artificial planting plants, roots are thick and solid, and the number of the roots are more.

Description

technical field [0001] The invention relates to a method for cultivating and transplanting seedlings of Taoerqi. Background technique [0002] peach seven ( Sinopodophyllum hexandrum Royle) is a herbaceous plant of the genus Peach of the family Berberidaceae, a rare medicinal plant in the world, and has been included in the "List of Rare and Endangered Plants in China", a national third-level protected plant. The podophyllotoxin contained in its roots and rhizomes is the starting material for the synthesis of various anticancer drugs. Under natural conditions, Taoerqi mainly reproduces by seeds, the seeds have a long dormant period (6-8 months), and grow very slowly after germination. Compared with some other perennial medicinal plants, its aerial part has a very short life cycle , the annual effective growth days are only 90-120 days, and it takes 5-6 years to grow into commercial medicinal materials. [0003] The distribution of Taoerqi is at an altitude of 1500-4300m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G31/00
Inventor 郭琪杨晖周剑平王治业张军杨涛王沛雅张建军赵小锋
Owner GANSU ACAD OF SCI INST OF BIOLOGY
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