Method for training tissue culture seedling of tomato

A technology of tissue culture seedlings and tomato, applied in the field of tomato cultivation, can solve the problems of large environmental changes, low survival rate of transplanting, unfavorable seedlings adapting to the new environment, etc., and achieve the effect of improving the survival rate

Inactive Publication Date: 2008-10-08
INST OF GARDENING ACAD OF AGRI OF SHANGHAI
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  • Summary
  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

The main defect is that the environment of the seedlings changes greatly, which is not conducive to the adaptation of the seedlings to the new environment, so the survival rate of transplanting is low
Therefore, people's needs cannot be met

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Adopt as Anhui Agricultural Sciences 2006,34 (13): 3020-3022 " establishment of tomato cotyledon regeneration system " document disclosed method, the tomato seed of yellow rose variety is cultivated and germinated in test tube, totally 100 strains, wait for tomato The roots of the test tube seedlings are stretched to 5cm, cover the test tube cover, then transfer the test tube to a greenhouse for adaptation for 1 day, remove the test tube cover, and add distilled water in the test tube. The amount of distilled water added is 1 / 4 of the volume of the test tube. Cover with non-woven fabric, and pour water on the non-woven fabric. After 3 days, the seedlings are taken out from the test tube, the culture medium at the root is washed, and transferred to a plug containing hardening medium to adapt for 7 days, and then planted in a medium containing hardening medium. The medium was adapted to the cultivation pot for 7 days, watered once a day, and the survival rate of the seedli...

Embodiment 2

[0021] Adopt as Anhui Agricultural Science 2006,34 (13): 3020-3022 " the establishment of tomato cotyledon regeneration system " document disclosed method, the tomato seed of Tianfu 501 kind is cultivated and germinated in test tube, altogether 100 plants, wait for tomato The roots of the test-tube seedlings are stretched to 6cm, cover the test tube cover, then transfer the test tube to a greenhouse to adapt to it for 2 days, remove the test tube cover, and add distilled water in the test tube. The amount of distilled water added is 1 / 3 of the test tube volume. Cover with non-woven fabric, and pour water on the non-woven fabric. After 2 days, take the seedlings out of the test tube, wash the medium of the roots, transfer them to the plugs containing the hardening medium and adapt to them for 7 days, and then plant them in the medium containing the hardening medium. The cultivation pot of the culture medium was adapted for 7 days, watered twice a day, and the survival rate of th...

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PUM

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Abstract

The invention discloses a culturing and refining seedling method of tomato tissue, including the following steps: tomato seedling is obtained by tissue culture, when the root of a tomato tube seedling grows to 5-6cm and the tube seedling grows to 6-7cm, the entire tube is firstly transferred into a greenhouse for adaptation for one day, then the tube is opened and filled with 0.5cm of distilled water, non-woven fabrics is covered on the tube and sprayed with water for adaptation for 1-2 days, then the non-woven fabrics is removed for adaptation for one day, the seedling, together with the root, is carefully taken out from the tube, culture medium at the root is washed away, then the seedling is transplanted in an aperture disk which is loaded with a medium prepared by vermiculite, perlite, peaty soil and organic fertilizer according to the volume ratio of 2:2:1:1 for adaptation for 7days, then is transplanted in a nutrition bowel with the same medium for adaptation for 7 days, and finally is transplanted in the field; the grading seedling adaptation method successfully overcomes the hurdle of transplant survival, and the transplant survival rate in the experiments can reach 100%.

Description

technical field [0001] The invention relates to a tomato cultivation method, in particular to an artificial cultivation method for tomato seedlings. Background technique [0002] Tomato is an important crop, which is rich in nutrition and has health care functions. It is one of the most important vegetables in the world. [0003] In the tomato planting process, the survival rate of seedlings is very important. At present, the conventional method is "Establishment of Tomato Efficient Regeneration System", Volume 20, No. 4, Journal of Hainan University, Natural Science Edition, December 2002. The steps are: remove the sealing film, place the rooted seedlings in a room at room temperature to harden them, take out the seedlings after 3 days, wash off the medium adhered to the roots, and put them in flower pots filled with sandy loam until they grow to 15 cm. High time shifts people to colonize in the field. The main defect is that the environment of the seedlings changes great...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G31/00A01C1/02
Inventor 朱为民王曙霞万延慧朱龙英
Owner INST OF GARDENING ACAD OF AGRI OF SHANGHAI
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