A kind of pcr instrument hot start method and pcr instrument with hot start function

Abstract

The invention solves the problem of providing a warm start method of a PCR (Polymerase Chain Reaction) instrument and a PCR instrument with a warm start function. The warm start method of the PCR instrument comprises the following steps of: setting an experimental reaction procedure to be tested of the PCR instrument; selecting a working manner of the PCR instrument, and selecting the following actions: room-temperature start and warm start; if the warm start is selected, setting the preheating temperature, and transmitting a heating signal through a processor so as to heat an aluminum base; judging whether the temperature of the aluminum base is heated to a preheating temperature set value, if not, continuing heating, after the aluminum base reaches the preheating temperature, placing a PCR reaction system prepared on ice in the aluminum base through an experimenter, and clicking to affirm to start the experiment; and operating the warm start PCR reaction. The invention has the advantages that: owing to adoption of the technical scheme, the warm start function is added into the PCR instrument, so that the PCR instrument is convenient and fast to operate; non-specific amplification of the PCR experiment is effectively reduced, and the PCR reaction is optimized.

Description

technical field [0001] The invention relates to the technical field of PCR instruments, in particular to a method for hot-starting a PCR instrument and a PCR instrument with a hot-start function. Background technique [0002] In the traditional PCR reaction, each component of the reaction system is added at one time and enters the cycle. Although the optimal extension temperature of TaqDNA polymerase is 72°C, the polymerase is still active at room temperature. Therefore, during the preparation of the PCR reaction, and at the beginning of the thermal cycle, when the incubation temperature is lower than the annealing temperature, primer mismatch and dimer formation can occur, which in turn leads to the amplification of non-specific products. [0003] Hot start is a necessary component of PCR reaction controlled by various physical and chemical methods, until the reaction mixture is heated to a temperature that can prevent non-specific guidance and primer polymerization, and th...

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Problems solved by technology

Add one less PCR reaction component, TaqDNA enzyme, primer, deoxynucleotide triphosphate (dNTP) or template DNA, and then add the component after the temperature reaches the reaction temperature. Throughput applications; the method of chemically modifying reaction components such as TaqDNA enzymes, primers, and deoxyribonucleotide triphosphates (dNTPs) is expensive to develop and use; a classic hot-start method is to prepare PCR reaction solutions on ice. When the DNA polymerase has no activity, it is placed in a preheated PCR instrument to start the reaction. This method is simple, cheap, and easy to popularize. However, the preheating operation of the existing PCR instrument is inaccurate, requires artificial monitoring and measurement, and is very cumbersome

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