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Glechoma longituba phenol, and preparation method and application thereof

A technology of fenugreek and medicinal plants, which is applied in the field of fenugreek and its preparation, can solve the problem that a certain compound or several compounds with no specific anticoagulation and thrombolytic effects are given, and the variety of compounds can be enriched. , good anti-oxidation, wide range of effects

Inactive Publication Date: 2014-08-06
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, this patent document does not specifically provide a certain compound or several compounds with anticoagulant and thrombolytic effects

Method used

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  • Glechoma longituba phenol, and preparation method and application thereof
  • Glechoma longituba phenol, and preparation method and application thereof
  • Glechoma longituba phenol, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056]The extraction separation of embodiment 1 compound I, II, III

[0057] (1) Extraction and separation

[0058] 1) Take 16.0kg of the dried whole herb of the medicinal plant Styracis chinensis, and ultrasonically extract it twice with distilled water, each time for 30 minutes. Then the filter residue was reflux extracted with water for 1 h, all the extracts were combined, and concentrated by rotary evaporation to obtain the extract;

[0059] 2) Suspend the extract with distilled water, and then repeatedly extract with ethyl acetate and n-butanol, respectively, to obtain the ethyl acetate extraction part and n-butanol extraction part, which are concentrated under reduced pressure and dried to obtain the ethyl acetate extraction part respectively. extracts and n-butanol extracts;

[0060] 3) The ethyl acetate extract was subjected to silica gel column chromatography, and gradient elution was carried out with ethyl acetate and methanol (the volume ratio of ethyl acetate to ...

Embodiment 2

[0087] The antioxidant activity of embodiment 2 compound I, II, III

[0088] (1) Experimental samples: cyclamol A, cyclamol B, cyclamol C;

[0089] (2) Experimental instruments and reagents: constant temperature shaking incubator, microplate reader, Vc, 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH), Milli-Q water;

[0090] (3) Experimental method: DPPH method was used to measure the antioxidant activity of Dichryonol A-C, and Vc was used as a positive drug to investigate the established model.

[0091] Dissolve Limobilol A-C in DMSO, and dilute with Milli-Q water to a gradient concentration of 50 μmol / L, 25 μmol / L, 12.5 μmol / L, 6.25 μmol / L, 3.125 μmol / L, and 1 μmol / L. Set the test group, background group, and control group, among which,

[0092] Test group: sample 100μL+DPPH100μL;

[0093] Blank group: 100 μL of sample + 100 μL of ethanol;

[0094] Control group: water 100μL+DPPH100μL;

[0095] After mixing, shake and incubate at 37°C for 1 h, measure the OD value at 517 ...

Embodiment 3

[0099] Example 3 Compound I, II, III to H 2 o 2 Protection from H9c2 Cardiomyocyte Injury

[0100] (1) Experimental samples: cyclamol A, cyclamol B, cyclamol C;

[0101] (2) Experimental equipment and reagents: H9c2 cardiomyocytes, thiazolium blue (MTT), DMEM high-glucose medium, fetal bovine serum, double antibodies, DMSO, CO 2 Incubator, inverted microscope, ultra-clean bench, plate shaker;

[0102] (3) Experimental method: Count H9c2 cardiomyocytes and culture them in 96-well flat-bottom culture plates; Respectively pre-protected, and then cultured in a cell culture incubator for 12h; then with 450μmol / L H 2 o 2Treat for 4 hours to create a model of cell oxidative damage, and use the MTT method to measure cell viability to observe the effect of the sample on H 2 o 2 Protection against oxidative damage in cardiomyocytes. Test results such as Figure 23 As shown, the Control group in the figure represents the survival rate of cardiomyocytes without hydrogen peroxide ...

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Abstract

The invention discloses glechoma longituba phenol, and a preparation method and application thereof. The glechoma longituba phenol is compound I, compound II or compound III; and the structural formula is shown in a figure 24. The preparation method comprises the steps of putting glechoma longituba (Glechoma longituba (NaKai) Kupr.) into a solvent to extract, obtaining leach liquor; extracting the leach liquor; concentrating extract liquor to obtain extract; and separating and purifying to obtain the glechoma longituba phenol from the extract. The application is application of the glechoma longituba phenol in preparation of an antioxidant drug and an anti-inflammatory analgesic drug. Compared with the prior art, three structures of the glechoma longituba phenol disclosed by the invention are compounds which are found out for the first time; the existing compound variety is enriched; and the glechoma longituba phenol disclosed by the invention has good antioxidant activity and anti-inflammatory activity, can be used for preparing the antioxidant drug, the anti-inflammatory analgesic drug and the like, and is wide in use.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to a bibialol and its preparation method and application. Background technique [0002] Glechoma longituba (Nakai) Kupr. is a dry whole herb of the Lamiaceae Huoxuedan genus. It is a perennial creeping herb that mostly grows in fields, forest edges, roadsides, forest meadows, streamsides or riversides. In the damp grass beside the village, it is mainly produced in Jiangsu and Zhejiang. [0003] According to the first part of the "Pharmacopoeia" 2010 edition, Lianqiancao has the effects of promoting dampness and treating stranguria, clearing away heat and detoxification, dispelling blood stasis and reducing swelling, and is a commonly used traditional Chinese medicine among the people. Clinically, it is mainly used for diuresis and treatment of bladder stones, kidney stones, ureteral stones, nephritis, edema, damp-heat jaundice, cholecystitis, mumps, sores, carbuncles, swelling, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C39/15C07C37/70A61K31/05A61P39/06A61P29/00
Inventor 瞿海斌程翼宇王毅朱求方
Owner ZHEJIANG UNIV
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