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Making method of rapid detection kit of dibutyl phthalate, and detection method of rapid detection kit of dibutyl phthalate

A technology of dibutyl phthalate and a kit, which is applied in the field of analysis and detection, can solve the problem of less phthalates, and achieve the effects of stable detection recovery rate, good promotion prospects, and accurate results

Inactive Publication Date: 2013-07-31
BEIJING PRIMEBIOTEK COMPANY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology allows quicker identification of DBP without having too much trouble or inconvenience from previous methods that require long hours before results are obtained. It also has several benefits over existing techniques such as accuracy, stability, ease of use and low costs.

Problems solved by technology

This patented technical problem addressed in this patent relates to developing a quick assay toolkit for identifying both chemical hazmatocytcs called dimer acid cyanide ester (DLCA) and diacrimonyltriphenamidium chlorophyll (DNCT)). Current methods require complicated procedures involving multiple steps including filtrative column separation, resealing, dissolution, and measurement under controlled conditions.

Method used

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  • Making method of rapid detection kit of dibutyl phthalate, and detection method of rapid detection kit of dibutyl phthalate
  • Making method of rapid detection kit of dibutyl phthalate, and detection method of rapid detection kit of dibutyl phthalate
  • Making method of rapid detection kit of dibutyl phthalate, and detection method of rapid detection kit of dibutyl phthalate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0081] Preparation of a rapid detection of dibutyl phthalate (DBP) ELISA kit

[0082] 1. Prepare dibutyl phthalate (DBP) standard solution:

[0083] Prepared with dibutyl phthalate (DBP) standard product, diluted with ultrapure water containing 10% methanol to six concentrations of 0ppm, 0.09ppm, 0.27ppm, 0.81ppm, 2.43ppm, and 7.29ppm, and divided into 6 bottle, stored at low temperature and protected from light.

[0084] 2. Pre-coat microplates with dibutyl phthalate (DBP) complete antigen:

[0085] 1) coated

[0086] Mix 0.05 mol / L coating solution and dibutyl phthalate (DBP) complete antigen in an appropriate ratio, load the mixed solution on the carrier, and let it pass through the solution at 4°C (about 16h).

[0087] 2) washing plate

[0088] Wash twice with phosphate buffer saline containing 0.5% Tween20 (volume).

[0089] 3) closed protection

[0090] Add 1% bovine serum albumin blocking protection solution to the washed solid phase carrier, 200 μL per well, and ...

Embodiment 2

[0106] Use the ELISA kit prepared by the embodiment of the present invention 1 to draw the DBP working curve

[0107] 1. Coating step

[0108] Mix 0.05 mol / L coating solution and dibutyl phthalate (DBP) complete antigen in an appropriate ratio, load the mixed solution on the carrier, and let it pass through the solution at 4°C (about 16h).

[0109] 2. Closure step

[0110] Add 1% bovine serum albumin blocking protection solution to the washed solid phase carrier, 200 μL per well, and let stand at 37° C. for 1 hour. Shake it into the protective solution, pat it dry with absorbent paper, and put it in a drying oven at 37°C for 1 hour. Put the dried ELISA plate into the aluminum foil bag of the kit, put a desiccant in each bag, and vacuum pack and seal.

[0111] 3. Competition step

[0112] Add 50 μL LDBP standard solution, 50 μL LDBP antibody and 50 μL enzyme-labeled secondary antibody to each well to make a competition reaction. After incubating at 37°C for 40 minutes, tak...

Embodiment 3

[0120] The method for detecting dibutyl phthalate (DBP) using the enzyme-linked immunosorbent assay kit prepared in Example 1 of the present invention and the standard curve drawn in Example 2.

[0121] 1. Sample pretreatment

[0122] 1.1 Bottled or barreled drinking water

[0123] Add concentrated sample diluent to dilute the sample to 1.5 times, which is the solution to be tested.

[0124] 1.2 Bottled or canned beverages and alcohol

[0125] Take 5ml of the sample and add 2ml of chromatographically pure n-hexane to mix well and then stand still. Take 1ml of the supernatant and evaporate it to dryness, and redissolve it with 1ml of 35% methanol, that is, dilute it to 0.4 times the test solution.

[0126] 2. Use the rapid detection dibutyl phthalate (DBP) ELISA kit to detect the content of dibutyl phthalate (DBP) in the sample.

[0127] 2.1 Take the kit out from 4°C, return to room temperature, add dibutyl phthalate (DBP) standard solution or test solution 50 μL / well, DBP a...

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Abstract

The invention discloses an enzyme-linked immunosorbent assay determination kit for detecting dibutyl phthalate (DBP). The kit comprises a DBP standard solution, a solid phase carrier, an enzyme marker, an anti-DBP reagent, a substrate coloring solution, a coating solution, an enzyme dilution operating solution, a stopping solution and a concentrated washing solution. The invention also discloses a making method of the enzyme-linked immunosorbent assay determination kit for detecting the DBP, and a detection method of the enzyme-linked immunosorbent assay determination kit for detecting the DBP. The DBP kit made in the invention has the characteristics of simple operation, high sensitivity, high sensitivity, good specificity, good linearity and the like.

Description

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Claims

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Application Information

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Owner BEIJING PRIMEBIOTEK COMPANY
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